PEC‐A: An immortalized porcine aortic endothelial cell
Identifieur interne : 000F07 ( Istex/Corpus ); précédent : 000F06; suivant : 000F08PEC‐A: An immortalized porcine aortic endothelial cell
Auteurs : Mehran M. Khodadoust ; Francisco J. Candal ; Stephen E. Maher ; Allan G. Murray ; Jordan S. Pober ; William C. Davis ; Edwin W. Ades ; Alfred L. M. BothwellSource :
- Xenotransplantation [ 0908-665X ] ; 1995-05.
Abstract
Abstract: Cultured porcine aortic endothelium was transfected with sequences that encode the SV40 T antigen, resulting in an immortalized cell line that retains the differentiated properties of normal aortic endothelial cells. Specifically, these cells form cobblestone monolayers, synthesize von Willebrand factor, and endocytose acetylated LDL. These cells can be readily propagated in culture and have been passaged over a year in culture. The cells form tubular structures when grown on Matrigel. The cells in culture express class I but do not express MHC class II antigens. Both class I and class II expression can be induced by treatment with recombinant swine interferon‐γ. Expression of CD44 and several other cell surface antigens have been observed. Co‐culture of these cells with purified human CD4+ T cells resulted in a significant T‐cell proliferative response similar to that observed for primary porcine EC. Finally, the cells are readily susceptible to transfection and express exogenous genes. These cells should be valuable for study of human anti‐porcine endothelial responses.
Url:
DOI: 10.1111/j.1399-3089.1995.tb00070.x
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Specifically, these cells form cobblestone monolayers, synthesize von Willebrand factor, and endocytose acetylated LDL. These cells can be readily propagated in culture and have been passaged over a year in culture. The cells form tubular structures when grown on Matrigel. The cells in culture express class I but do not express MHC class II antigens. Both class I and class II expression can be induced by treatment with recombinant swine interferon‐γ. Expression of CD44 and several other cell surface antigens have been observed. Co‐culture of these cells with purified human CD4+ T cells resulted in a significant T‐cell proliferative response similar to that observed for primary porcine EC. Finally, the cells are readily susceptible to transfection and express exogenous genes. These cells should be valuable for study of human anti‐porcine endothelial responses.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Mehran M. 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number="79">79</numbering><numbering type="pageLast" number="87">87</numbering></numberingGroup><correspondenceTo>Section of Immunobiology, PO Box 208011, Yale University School of Medicine, 310 Cedar St. New Haven, CT 06520–8011, USA.</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:XEN.XEN79.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Accepted 2 February 1995.</unparsedEditorialHistory><countGroup><count type="referenceTotal" number="13"></count><count type="linksCrossRef" number="9"></count></countGroup><titleGroup><title type="main">PEC‐A: An immortalized porcine aortic endothelial cell</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1"><personName><givenNames>Mehran M.</givenNames><familyName>Khodadoust</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a2"><personName><givenNames>Francisco J.</givenNames><familyName>Candal</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1"><personName><givenNames>Stephen E.</givenNames><familyName>Maher</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a3"><personName><givenNames>Allan G.</givenNames><familyName>Murray</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a3"><personName><givenNames>Jordan S.</givenNames><familyName>Pober</familyName></personName></creator><creator creatorRole="author" xml:id="cr6" affiliationRef="#a4"><personName><givenNames>William C.</givenNames><familyName>Davis</familyName></personName></creator><creator creatorRole="author" xml:id="cr7" affiliationRef="#a2"><personName><givenNames>Edwin W.</givenNames><familyName>Ades</familyName></personName></creator><creator creatorRole="author" xml:id="cr8" affiliationRef="#a1" corresponding="yes"><personName><givenNames>Alfred L.M.</givenNames><familyName>Bothwell</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="US"><unparsedAffiliation>Section of Immunobiology and Department of Biology, Yale University School of Medicine, New Haven, CT, USA</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="US"><unparsedAffiliation>Biological Products Branch, National Center for Infectious Diseases, Centers for Disease Control, Atlanta, GA, USA</unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="US"><unparsedAffiliation>Molecular Cardiobiology Program, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, CT, USA</unparsedAffiliation></affiliation><affiliation xml:id="a4" countryCode="US"><unparsedAffiliation>Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA, USA</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">endothelium</keyword><keyword xml:id="k2">transformed cell</keyword><keyword xml:id="k3">porcine γ‐interferon</keyword><keyword xml:id="k4">porcine MHC</keyword><keyword xml:id="k5">porcine‐specific antibodies</keyword><keyword xml:id="k6">VWF expression</keyword><keyword xml:id="k7">tubule formation</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><p><b>Abstract: </b> Cultured porcine aortic endothelium was transfected with sequences that encode the SV40 T antigen, resulting in an immortalized cell line that retains the differentiated properties of normal aortic endothelial cells. Specifically, these cells form cobblestone monolayers, synthesize von Willebrand factor, and endocytose acetylated LDL. These cells can be readily propagated in culture and have been passaged over a year in culture. The cells form tubular structures when grown on Matrigel. The cells in culture express class I but do not express MHC class II antigens. Both class I and class II expression can be induced by treatment with recombinant swine interferon‐γ. Expression of CD44 and several other cell surface antigens have been observed. Co‐culture of these cells with purified human CD4+ T cells resulted in a significant T‐cell proliferative response similar to that observed for primary porcine EC. Finally, the cells are readily susceptible to transfection and express exogenous genes. These cells should be valuable for study of human anti‐porcine endothelial responses.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>PEC‐A: An immortalized porcine aortic endothelial cell</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>PEC‐A: An immortalized porcine aortic endothelial cell</title></titleInfo><name type="personal"><namePart type="given">Mehran M.</namePart><namePart type="family">Khodadoust</namePart><affiliation>Section of Immunobiology and Department of Biology, Yale University School of Medicine, New Haven, CT, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Francisco J.</namePart><namePart type="family">Candal</namePart><affiliation>Biological Products Branch, National Center for Infectious Diseases, Centers for Disease Control, Atlanta, GA, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Stephen E.</namePart><namePart type="family">Maher</namePart><affiliation>Section of Immunobiology and Department of Biology, Yale University School of Medicine, New Haven, CT, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Allan G.</namePart><namePart type="family">Murray</namePart><affiliation>Molecular Cardiobiology Program, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, CT, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jordan S.</namePart><namePart type="family">Pober</namePart><affiliation>Molecular Cardiobiology Program, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, CT, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">William C.</namePart><namePart type="family">Davis</namePart><affiliation>Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Edwin W.</namePart><namePart type="family">Ades</namePart><affiliation>Biological Products Branch, National Center for Infectious Diseases, Centers for Disease Control, Atlanta, GA, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Alfred L.M.</namePart><namePart type="family">Bothwell</namePart><affiliation>Section of Immunobiology and Department of Biology, Yale University School of Medicine, New Haven, CT, USA</affiliation><affiliation>Correspondence address: Section of Immunobiology, PO Box 208011, Yale University School of Medicine, 310 Cedar St. New Haven, CT 06520–8011, USA.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">1995-05</dateIssued><edition>Accepted 2 February 1995.</edition><copyrightDate encoding="w3cdtf">1995</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><extent unit="references">13</extent><extent unit="linksCrossRef">9</extent></physicalDescription><abstract lang="en">Abstract: Cultured porcine aortic endothelium was transfected with sequences that encode the SV40 T antigen, resulting in an immortalized cell line that retains the differentiated properties of normal aortic endothelial cells. Specifically, these cells form cobblestone monolayers, synthesize von Willebrand factor, and endocytose acetylated LDL. These cells can be readily propagated in culture and have been passaged over a year in culture. The cells form tubular structures when grown on Matrigel. The cells in culture express class I but do not express MHC class II antigens. Both class I and class II expression can be induced by treatment with recombinant swine interferon‐γ. Expression of CD44 and several other cell surface antigens have been observed. Co‐culture of these cells with purified human CD4+ T cells resulted in a significant T‐cell proliferative response similar to that observed for primary porcine EC. Finally, the cells are readily susceptible to transfection and express exogenous genes. These cells should be valuable for study of human anti‐porcine endothelial responses.</abstract><subject lang="en"><genre>keywords</genre><topic>endothelium</topic><topic>transformed cell</topic><topic>porcine γ‐interferon</topic><topic>porcine MHC</topic><topic>porcine‐specific antibodies</topic><topic>VWF expression</topic><topic>tubule formation</topic></subject><relatedItem type="host"><titleInfo><title>Xenotransplantation</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><identifier type="ISSN">0908-665X</identifier><identifier type="eISSN">1399-3089</identifier><identifier type="DOI">10.1111/(ISSN)1399-3089</identifier><identifier type="PublisherID">XEN</identifier><part><date>1995</date><detail type="volume"><caption>vol.</caption><number>2</number></detail><detail type="issue"><caption>no.</caption><number>2</number></detail><extent unit="pages"><start>79</start><end>87</end><total>9</total></extent></part></relatedItem><relatedItem type="references" displayLabel="cit1"><titleInfo><title>Stimulation of lymphocyte proliferation by non‐lymphoid porcine tissue cells</title></titleInfo><name type="personal"><namePart type="given">G</namePart><namePart type="family">Pawelec</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">HS</namePart><namePart type="family">Davies</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JD</namePart><namePart type="family">Pearson</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C</namePart><namePart type="family">Steele</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="family">Brons</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Pawelec G, Davies HS, Pearson JD, Steele C, Brons G. 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Transplantation 1994:57:1709.</note><part><date>1994</date><detail type="volume"><caption>vol.</caption><number>57</number></detail><extent unit="pages"><start>1709</start></extent></part><relatedItem type="host"><titleInfo><title>Transplantation</title></titleInfo><part><date>1994</date><detail type="volume"><caption>vol.</caption><number>57</number></detail><extent unit="pages"><start>1709</start></extent></part></relatedItem></relatedItem><identifier type="istex">B4E453E3E11D5F24A18227F0C5858A2C7C4D35FD</identifier><identifier type="ark">ark:/67375/WNG-J3F0GFQ4-C</identifier><identifier type="DOI">10.1111/j.1399-3089.1995.tb00070.x</identifier><identifier type="ArticleID">XEN79</identifier><accessCondition type="use and reproduction" contentType="copyright">© 1995 Munksgaard</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-L0C46X92-X">wiley</recordContentSource><recordOrigin>Converted from (version ) to MODS version 3.6.</recordOrigin><recordCreationDate encoding="w3cdtf">2019-11-16</recordCreationDate></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-J3F0GFQ4-C/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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