Characterization of the replication-competent porcine endogenous retrovirus class B molecular clone originated from Korean domestic pig
Identifieur interne : 000D16 ( Istex/Corpus ); précédent : 000D15; suivant : 000D17Characterization of the replication-competent porcine endogenous retrovirus class B molecular clone originated from Korean domestic pig
Auteurs : Na Young Kim ; Donghee Lee ; Jungeun Lee ; Eung Woo Park ; Woon-Won Jung ; Jai Myung Yang ; Young Bong KimSource :
- Virus Genes [ 0920-8569 ] ; 2009-10-01.
English descriptors
Abstract
Abstract: Xenotransplantation from pigs offers an opportunity to resolve the shortage of human organs. The porcine endogenous retrovirus (PERV) cannot be eliminated because of its presence in the germline DNA. Three subgroups of the replication-competent PERV (PERV-A, PERV-B, and PERV-C) have been identified in pigs. We constructed a molecular clone of PERV-B from a Korean domestic pig BAC clone containing PERV genomes, and its replication competency was characterized in human cells. The pol region of PERV-B was detected in the genomic DNA of 293T cells transfected with PERV-B (465D1) and in the genomic DNA of 293T cells infected with PERV-B (465D1) viruses. The 293T cells transfected with PERV-B (465D1) were maintained for 140 days. PERV-B (465D1) showed the stationary replication competence with no toxicity in the cell growth.
Url:
DOI: 10.1007/s11262-009-0377-7
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ISTEX:39D32A39FACA88D2117D2BF9361CA1F06DE3461ALe document en format XML
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Korea</mods:affiliation></affiliation></author><author><name sortKey="Yang, Jai Myung" sort="Yang, Jai Myung" uniqKey="Yang J" first="Jai Myung" last="Yang">Jai Myung Yang</name><affiliation><mods:affiliation>Department of Life Science, Sogang University, 121-742, Seoul, South Korea</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: jaimyang@sogang.ac.kr</mods:affiliation></affiliation></author><author><name sortKey="Kim, Young Bong" sort="Kim, Young Bong" uniqKey="Kim Y" first="Young Bong" last="Kim">Young Bong Kim</name><affiliation><mods:affiliation>Department of Animal Biotechnology, College of Animal Bioscience & Technology, Konkuk University, 143-701, Seoul, South Korea</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: kimera@konkuk.ac.kr</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Virus Genes</title><title level="j" type="abbrev">Virus Genes</title><idno type="ISSN">0920-8569</idno><idno type="eISSN">1572-994X</idno><imprint><publisher>Springer US; http://www.springer-ny.com</publisher><pubPlace>Boston</pubPlace><date type="published" when="2009-10-01">2009-10-01</date><biblScope unit="volume">39</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="210">210</biblScope><biblScope unit="page" to="216">216</biblScope></imprint><idno type="ISSN">0920-8569</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0920-8569</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Molecular clone</term><term>PERV</term><term>Replication competency</term><term>Xenotransplantation</term><term>Zoonosis</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Xenotransplantation from pigs offers an opportunity to resolve the shortage of human organs. The porcine endogenous retrovirus (PERV) cannot be eliminated because of its presence in the germline DNA. Three subgroups of the replication-competent PERV (PERV-A, PERV-B, and PERV-C) have been identified in pigs. We constructed a molecular clone of PERV-B from a Korean domestic pig BAC clone containing PERV genomes, and its replication competency was characterized in human cells. The pol region of PERV-B was detected in the genomic DNA of 293T cells transfected with PERV-B (465D1) and in the genomic DNA of 293T cells infected with PERV-B (465D1) viruses. The 293T cells transfected with PERV-B (465D1) were maintained for 140 days. PERV-B (465D1) showed the stationary replication competence with no toxicity in the cell growth.</div></front></TEI><istex><corpusName>springer-journals</corpusName><author><json:item><name>Na Young Kim</name><affiliations><json:string>Department of Life Science, Sogang University, 121-742, Seoul, South Korea</json:string><json:string>Department of Animal Biotechnology, College of Animal Bioscience & Technology, Konkuk University, 143-701, Seoul, South Korea</json:string></affiliations></json:item><json:item><name>Donghee Lee</name><affiliations><json:string>Department of Animal Biotechnology, College of Animal Bioscience & Technology, Konkuk University, 143-701, Seoul, South Korea</json:string></affiliations></json:item><json:item><name>Jungeun Lee</name><affiliations><json:string>Department of Animal Biotechnology, College of Animal Bioscience & Technology, Konkuk University, 143-701, Seoul, South Korea</json:string></affiliations></json:item><json:item><name>Eung Woo Park</name><affiliations><json:string>National Livestock Research Institute, Suwon, South Korea</json:string></affiliations></json:item><json:item><name>Woon-Won Jung</name><affiliations><json:string>My-Gene Bioscience Institute, Seoul, South Korea</json:string></affiliations></json:item><json:item><name>Jai Myung Yang</name><affiliations><json:string>Department of Life Science, Sogang University, 121-742, Seoul, South Korea</json:string><json:string>E-mail: jaimyang@sogang.ac.kr</json:string></affiliations></json:item><json:item><name>Young Bong Kim</name><affiliations><json:string>Department of Animal Biotechnology, College of Animal Bioscience & Technology, Konkuk University, 143-701, Seoul, South Korea</json:string><json:string>E-mail: kimera@konkuk.ac.kr</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Xenotransplantation</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Zoonosis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PERV</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Molecular clone</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Replication competency</value></json:item></subject><articleId><json:string>377</json:string><json:string>s11262-009-0377-7</json:string></articleId><arkIstex>ark:/67375/VQC-N5XLXSTW-C</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Abstract: Xenotransplantation from pigs offers an opportunity to resolve the shortage of human organs. The porcine endogenous retrovirus (PERV) cannot be eliminated because of its presence in the germline DNA. Three subgroups of the replication-competent PERV (PERV-A, PERV-B, and PERV-C) have been identified in pigs. We constructed a molecular clone of PERV-B from a Korean domestic pig BAC clone containing PERV genomes, and its replication competency was characterized in human cells. The pol region of PERV-B was detected in the genomic DNA of 293T cells transfected with PERV-B (465D1) and in the genomic DNA of 293T cells infected with PERV-B (465D1) viruses. The 293T cells transfected with PERV-B (465D1) were maintained for 140 days. 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The porcine endogenous retrovirus (PERV) cannot be eliminated because of its presence in the germline DNA. Three subgroups of the replication-competent PERV (PERV-A, PERV-B, and PERV-C) have been identified in pigs. We constructed a molecular clone of PERV-B from a Korean domestic pig BAC clone containing PERV genomes, and its replication competency was characterized in human cells. The pol region of PERV-B was detected in the genomic DNA of 293T cells transfected with PERV-B (465D1) and in the genomic DNA of 293T cells infected with PERV-B (465D1) viruses. The 293T cells transfected with PERV-B (465D1) were maintained for 140 days. PERV-B (465D1) showed the stationary replication competence with no toxicity in the cell growth.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Xenotransplantation</term></item><item><term>Zoonosis</term></item><item><term>PERV</term></item><item><term>Molecular clone</term></item><item><term>Replication competency</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>Biomedicine</head><item><term>Plant Sciences</term></item><item><term>Virology</term></item><item><term>Medical Microbiology</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2008-11-11">Created</change><change 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molecular clone originated from Korean domestic pig</ArticleTitle><ArticleFirstPage>210</ArticleFirstPage><ArticleLastPage>216</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2009</Year><Month>6</Month><Day>6</Day></RegistrationDate><Received><Year>2008</Year><Month>11</Month><Day>11</Day></Received><Accepted><Year>2009</Year><Month>6</Month><Day>6</Day></Accepted><OnlineDate><Year>2009</Year><Month>6</Month><Day>19</Day></OnlineDate></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer Science+Business Media, LLC</CopyrightHolderName><CopyrightYear>2009</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1 Aff2"><AuthorName DisplayOrder="Western"><GivenName>Na</GivenName><GivenName>Young</GivenName><FamilyName>Kim</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Donghee</GivenName><FamilyName>Lee</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Jungeun</GivenName><FamilyName>Lee</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff3"><AuthorName DisplayOrder="Western"><GivenName>Eung</GivenName><GivenName>Woo</GivenName><FamilyName>Park</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff4"><AuthorName DisplayOrder="Western"><GivenName>Woon-Won</GivenName><FamilyName>Jung</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Jai</GivenName><GivenName>Myung</GivenName><FamilyName>Yang</FamilyName></AuthorName><Contact><Email>jaimyang@sogang.ac.kr</Email></Contact></Author><Author AffiliationIDS="Aff2" CorrespondingAffiliationID="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Young</GivenName><GivenName>Bong</GivenName><FamilyName>Kim</FamilyName></AuthorName><Contact><Phone>+82-2-4504208</Phone><Fax>+82-2-4551044</Fax><Email>kimera@konkuk.ac.kr</Email></Contact></Author><Affiliation ID="Aff1"><OrgDivision>Department of Life Science</OrgDivision><OrgName>Sogang University</OrgName><OrgAddress><City>Seoul</City><Postcode>121-742</Postcode><Country Code="KR">South Korea</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>Department of Animal Biotechnology, College of Animal Bioscience & Technology</OrgDivision><OrgName>Konkuk University</OrgName><OrgAddress><City>Seoul</City><Postcode>143-701</Postcode><Country Code="KR">South Korea</Country></OrgAddress></Affiliation><Affiliation ID="Aff3"><OrgName>National Livestock Research Institute</OrgName><OrgAddress><City>Suwon</City><Country Code="KR">South Korea</Country></OrgAddress></Affiliation><Affiliation ID="Aff4"><OrgName>My-Gene Bioscience Institute</OrgName><OrgAddress><City>Seoul</City><Country Code="KR">South Korea</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En" OutputMedium="All"><Heading>Abstract</Heading><Para TextBreak="No">Xenotransplantation from pigs offers an opportunity to resolve the shortage of human organs. The porcine endogenous retrovirus (PERV) cannot be eliminated because of its presence in the germline DNA. Three subgroups of the replication-competent PERV (PERV-A, PERV-B, and PERV-C) have been identified in pigs. We constructed a molecular clone of PERV-B from a Korean domestic pig BAC clone containing PERV genomes, and its replication competency was characterized in human cells. The <Emphasis Type="Italic">pol</Emphasis> region of PERV-B was detected in the genomic DNA of 293T cells transfected with PERV-B (465D1) and in the genomic DNA of 293T cells infected with PERV-B (465D1) viruses. The 293T cells transfected with PERV-B (465D1) were maintained for 140 days. PERV-B (465D1) showed the stationary replication competence with no toxicity in the cell growth.</Para></Abstract><KeywordGroup Language="En" OutputMedium="All"><Heading>Keywords</Heading><Keyword>Xenotransplantation</Keyword><Keyword>Zoonosis</Keyword><Keyword>PERV</Keyword><Keyword>Molecular clone</Keyword><Keyword>Replication competency</Keyword></KeywordGroup><ArticleNote Type="ESMHint"><Heading>Electronic supplementary material</Heading><SimplePara>The online version of this article (doi:<ExternalRef><RefSource>10.1007/s11262-009-0377-7</RefSource><RefTarget Address="10.1007/s11262-009-0377-7" TargetType="DOI"></RefTarget></ExternalRef>) contains supplementary material, which is available to authorized users.</SimplePara></ArticleNote></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Characterization of the replication-competent porcine endogenous retrovirus class B molecular clone originated from Korean domestic pig</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Characterization of the replication-competent porcine endogenous retrovirus class B molecular clone originated from Korean domestic pig</title></titleInfo><name type="personal"><namePart type="given">Na</namePart><namePart type="given">Young</namePart><namePart type="family">Kim</namePart><affiliation>Department of Life Science, Sogang University, 121-742, Seoul, South Korea</affiliation><affiliation>Department of Animal Biotechnology, College of Animal Bioscience & Technology, Konkuk University, 143-701, Seoul, South Korea</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Donghee</namePart><namePart type="family">Lee</namePart><affiliation>Department of Animal Biotechnology, College of Animal Bioscience & Technology, Konkuk University, 143-701, Seoul, South Korea</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jungeun</namePart><namePart type="family">Lee</namePart><affiliation>Department of Animal Biotechnology, College of Animal Bioscience & Technology, Konkuk University, 143-701, Seoul, South Korea</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Eung</namePart><namePart type="given">Woo</namePart><namePart type="family">Park</namePart><affiliation>National Livestock Research Institute, Suwon, South Korea</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Woon-Won</namePart><namePart type="family">Jung</namePart><affiliation>My-Gene Bioscience Institute, Seoul, South Korea</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Jai</namePart><namePart type="given">Myung</namePart><namePart type="family">Yang</namePart><affiliation>Department of Life Science, Sogang University, 121-742, Seoul, South Korea</affiliation><affiliation>E-mail: jaimyang@sogang.ac.kr</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Young</namePart><namePart type="given">Bong</namePart><namePart type="family">Kim</namePart><affiliation>Department of Animal Biotechnology, College of Animal Bioscience & Technology, Konkuk University, 143-701, Seoul, South Korea</affiliation><affiliation>E-mail: kimera@konkuk.ac.kr</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer US; http://www.springer-ny.com</publisher><place><placeTerm type="text">Boston</placeTerm></place><dateCreated encoding="w3cdtf">2008-11-11</dateCreated><dateIssued encoding="w3cdtf">2009-10-01</dateIssued><copyrightDate encoding="w3cdtf">2009</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: Xenotransplantation from pigs offers an opportunity to resolve the shortage of human organs. The porcine endogenous retrovirus (PERV) cannot be eliminated because of its presence in the germline DNA. Three subgroups of the replication-competent PERV (PERV-A, PERV-B, and PERV-C) have been identified in pigs. We constructed a molecular clone of PERV-B from a Korean domestic pig BAC clone containing PERV genomes, and its replication competency was characterized in human cells. The pol region of PERV-B was detected in the genomic DNA of 293T cells transfected with PERV-B (465D1) and in the genomic DNA of 293T cells infected with PERV-B (465D1) viruses. The 293T cells transfected with PERV-B (465D1) were maintained for 140 days. PERV-B (465D1) showed the stationary replication competence with no toxicity in the cell growth.</abstract><subject lang="en"><genre>Keywords</genre><topic>Xenotransplantation</topic><topic>Zoonosis</topic><topic>PERV</topic><topic>Molecular clone</topic><topic>Replication competency</topic></subject><relatedItem type="host"><titleInfo><title>Virus Genes</title></titleInfo><titleInfo type="abbreviated"><title>Virus Genes</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">2009-08-12</dateIssued><copyrightDate encoding="w3cdtf">2009</copyrightDate></originInfo><subject><genre>Biomedicine</genre><topic>Plant Sciences</topic><topic>Virology</topic><topic>Medical Microbiology</topic></subject><identifier type="ISSN">0920-8569</identifier><identifier type="eISSN">1572-994X</identifier><identifier type="JournalID">11262</identifier><identifier type="IssueArticleCount">16</identifier><identifier type="VolumeIssueCount">3</identifier><part><date>2009</date><detail type="volume"><number>39</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="pages"><start>210</start><end>216</end></extent></part><recordInfo><recordOrigin>Springer Science+Business Media, LLC, 2009</recordOrigin></recordInfo></relatedItem><identifier type="istex">39D32A39FACA88D2117D2BF9361CA1F06DE3461A</identifier><identifier type="ark">ark:/67375/VQC-N5XLXSTW-C</identifier><identifier type="DOI">10.1007/s11262-009-0377-7</identifier><identifier type="ArticleID">377</identifier><identifier type="ArticleID">s11262-009-0377-7</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer Science+Business Media, LLC, 2009</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Springer Science+Business Media, LLC, 2009</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-N5XLXSTW-C/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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