Demonstration of Circularization of Herpes Simplex Virus DNA Following infection Using Pulsed Field Gel Electrophoresis
Identifieur interne : 000C99 ( Istex/Corpus ); précédent : 000C98; suivant : 000D00Demonstration of Circularization of Herpes Simplex Virus DNA Following infection Using Pulsed Field Gel Electrophoresis
Auteurs : David A. Garber ; Stephen M. Beverley ; Donald M. CoenSource :
- Virology [ 0042-6822 ] ; 1993.
Abstract
Abstract: Studies of the configuration of intracellular herpes simplex virus DNA have been limited by the inability of restriction enzyme analyses to distinguish circular DNA from other configurations. To address this issue, we used pulsed-field gel electrophoresis of virus-infected, cycloheximide-treated cells and detected accumulation of viral DNA that failed to migrate out of the sample wells of the gels. This DNA lacked terminal restriction fragments and could be converted to unit-length linear species by γ-irradiation, demonstrating the circularization of viral DNA following infection.
Url:
DOI: 10.1006/viro.1993.1612
Links to Exploration step
ISTEX:56594F325E3DF7425FE1D616E0F8C07B05F945F3Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Demonstration of Circularization of Herpes Simplex Virus DNA Following infection Using Pulsed Field Gel Electrophoresis</title><author><name sortKey="Garber, David A" sort="Garber, David A" uniqKey="Garber D" first="David A." last="Garber">David A. Garber</name><affiliation><mods:affiliation>Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115</mods:affiliation></affiliation></author><author><name sortKey="Beverley, Stephen M" sort="Beverley, Stephen M" uniqKey="Beverley S" first="Stephen M." last="Beverley">Stephen M. Beverley</name><affiliation><mods:affiliation>Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115</mods:affiliation></affiliation></author><author><name sortKey="Coen, Donald M" sort="Coen, Donald M" uniqKey="Coen D" first="Donald M." last="Coen">Donald M. Coen</name><affiliation><mods:affiliation>Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:56594F325E3DF7425FE1D616E0F8C07B05F945F3</idno><date when="1993" year="1993">1993</date><idno type="doi">10.1006/viro.1993.1612</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-FBV19BHV-4/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000C99</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000C99</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Demonstration of Circularization of Herpes Simplex Virus DNA Following infection Using Pulsed Field Gel Electrophoresis</title><author><name sortKey="Garber, David A" sort="Garber, David A" uniqKey="Garber D" first="David A." last="Garber">David A. Garber</name><affiliation><mods:affiliation>Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115</mods:affiliation></affiliation></author><author><name sortKey="Beverley, Stephen M" sort="Beverley, Stephen M" uniqKey="Beverley S" first="Stephen M." last="Beverley">Stephen M. Beverley</name><affiliation><mods:affiliation>Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115</mods:affiliation></affiliation></author><author><name sortKey="Coen, Donald M" sort="Coen, Donald M" uniqKey="Coen D" first="Donald M." last="Coen">Donald M. Coen</name><affiliation><mods:affiliation>Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1993">1993</date><biblScope unit="volume">197</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="459">459</biblScope><biblScope unit="page" to="462">462</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Studies of the configuration of intracellular herpes simplex virus DNA have been limited by the inability of restriction enzyme analyses to distinguish circular DNA from other configurations. To address this issue, we used pulsed-field gel electrophoresis of virus-infected, cycloheximide-treated cells and detected accumulation of viral DNA that failed to migrate out of the sample wells of the gels. This DNA lacked terminal restriction fragments and could be converted to unit-length linear species by γ-irradiation, demonstrating the circularization of viral DNA following infection.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>David A. Garber</name><affiliations><json:string>Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115</json:string></affiliations></json:item><json:item><name>Stephen M. Beverley</name><affiliations><json:string>Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115</json:string></affiliations></json:item><json:item><name>Donald M. Coen</name><affiliations><json:string>Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-FBV19BHV-4</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Short communication</json:string></originalGenre><abstract>Abstract: Studies of the configuration of intracellular herpes simplex virus DNA have been limited by the inability of restriction enzyme analyses to distinguish circular DNA from other configurations. To address this issue, we used pulsed-field gel electrophoresis of virus-infected, cycloheximide-treated cells and detected accumulation of viral DNA that failed to migrate out of the sample wells of the gels. This DNA lacked terminal restriction fragments and could be converted to unit-length linear species by γ-irradiation, demonstrating the circularization of viral DNA following infection.</abstract><qualityIndicators><score>3.046</score><pdfWordCount>0</pdfWordCount><pdfCharCount>0</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>4</pdfPageCount><pdfPageSize>610 x 801 pts</pdfPageSize><refBibsNative>false</refBibsNative><abstractWordCount>83</abstractWordCount><abstractCharCount>597</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Demonstration of Circularization of Herpes Simplex Virus DNA Following infection Using Pulsed Field Gel Electrophoresis</title><pmid><json:string>8212585</json:string></pmid><pii><json:string>S0042-6822(83)71612-0</json:string></pii><genre><json:string>brief-communication</json:string></genre><host><title>Virology</title><language><json:string>unknown</json:string></language><publicationDate>1993</publicationDate><issn><json:string>0042-6822</json:string></issn><pii><json:string>S0042-6822(00)X0180-8</json:string></pii><volume>197</volume><issue>1</issue><pages><first>459</first><last>462</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date></date><geogName></geogName><orgName></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName></persName><placeName></placeName><ref_url></ref_url><ref_bibl></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-FBV19BHV-4</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - virology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - virology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Virology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string></inist></categories><publicationDate>1993</publicationDate><copyrightDate>1993</copyrightDate><doi><json:string>10.1006/viro.1993.1612</json:string></doi><id>56594F325E3DF7425FE1D616E0F8C07B05F945F3</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-FBV19BHV-4/fulltext.pdf</uri></json:item><json:item><extension>ocr</extension><original>false</original><mimetype>text/ocr</mimetype><quality><totalToken>2689</totalToken><correct>2377</correct><rate>78.68639310155247</rate><misspelled>310</misspelled></quality><langDetect><reliable>true</reliable><languages><json:item><score>1011</score><code>en</code><name>ENGLISH</name><percent>99</percent></json:item></languages></langDetect><uri>https://api.istex.fr/ark:/67375/6H6-FBV19BHV-4/fulltext.ocr</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-FBV19BHV-4/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-FBV19BHV-4/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Demonstration of Circularization of Herpes Simplex Virus DNA Following infection Using Pulsed Field Gel Electrophoresis</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>©1993 Academic Press</p></licence><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</p></availability><date>1993</date></publicationStmt><notesStmt><note type="brief-communication" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-S9SX2MFS-0">brief-communication</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note type="content">Section title: Short Communications</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Demonstration of Circularization of Herpes Simplex Virus DNA Following infection Using Pulsed Field Gel Electrophoresis</title><author xml:id="author-0000"><persName><forename type="first">David A.</forename><surname>Garber</surname></persName><affiliation>Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Stephen M.</forename><surname>Beverley</surname></persName><affiliation>Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Donald M.</forename><surname>Coen</surname></persName><affiliation>Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115</affiliation></author><idno type="istex">56594F325E3DF7425FE1D616E0F8C07B05F945F3</idno><idno type="ark">ark:/67375/6H6-FBV19BHV-4</idno><idno type="DOI">10.1006/viro.1993.1612</idno><idno type="PII">S0042-6822(83)71612-0</idno></analytic><monogr><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="pISSN">0042-6822</idno><idno type="PII">S0042-6822(00)X0180-8</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1993"></date><biblScope unit="volume">197</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="459">459</biblScope><biblScope unit="page" to="462">462</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1993</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Studies of the configuration of intracellular herpes simplex virus DNA have been limited by the inability of restriction enzyme analyses to distinguish circular DNA from other configurations. To address this issue, we used pulsed-field gel electrophoresis of virus-infected, cycloheximide-treated cells and detected accumulation of viral DNA that failed to migrate out of the sample wells of the gels. This DNA lacked terminal restriction fragments and could be converted to unit-length linear species by γ-irradiation, demonstrating the circularization of viral DNA following infection.</p></abstract></profileDesc><revisionDesc><change when="1993">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-FBV19BHV-4/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier converted-article found"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="sco" xml:lang="en"><item-info><jid>YVIRO</jid><aid>71612</aid><ce:pii>S0042-6822(83)71612-0</ce:pii><ce:doi>10.1006/viro.1993.1612</ce:doi><ce:copyright type="full-transfer" year="1993">Academic Press</ce:copyright></item-info><head><ce:dochead><ce:textfn>Short Communications</ce:textfn></ce:dochead><ce:title>Demonstration of Circularization of Herpes Simplex Virus DNA Following infection Using Pulsed Field Gel Electrophoresis</ce:title><ce:author-group><ce:author><ce:given-name>David A.</ce:given-name><ce:surname>Garber</ce:surname></ce:author><ce:author><ce:given-name>Stephen M.</ce:given-name><ce:surname>Beverley</ce:surname></ce:author><ce:author><ce:given-name>Donald M.</ce:given-name><ce:surname>Coen</ce:surname></ce:author><ce:affiliation><ce:textfn>Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115</ce:textfn></ce:affiliation></ce:author-group><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Studies of the configuration of intracellular herpes simplex virus DNA have been limited by the inability of restriction enzyme analyses to distinguish circular DNA from other configurations. To address this issue, we used pulsed-field gel electrophoresis of virus-infected, cycloheximide-treated cells and detected accumulation of viral DNA that failed to migrate out of the sample wells of the gels. This DNA lacked terminal restriction fragments and could be converted to unit-length linear species by γ-irradiation, demonstrating the circularization of viral DNA following infection.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Demonstration of Circularization of Herpes Simplex Virus DNA Following infection Using Pulsed Field Gel Electrophoresis</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Demonstration of Circularization of Herpes Simplex Virus DNA Following infection Using Pulsed Field Gel Electrophoresis</title></titleInfo><name type="personal"><namePart type="given">David A.</namePart><namePart type="family">Garber</namePart><affiliation>Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Stephen M.</namePart><namePart type="family">Beverley</namePart><affiliation>Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Donald M.</namePart><namePart type="family">Coen</namePart><affiliation>Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="brief-communication" displayLabel="Short communication" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-S9SX2MFS-0">brief-communication</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1993</dateIssued><copyrightDate encoding="w3cdtf">1993</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Studies of the configuration of intracellular herpes simplex virus DNA have been limited by the inability of restriction enzyme analyses to distinguish circular DNA from other configurations. To address this issue, we used pulsed-field gel electrophoresis of virus-infected, cycloheximide-treated cells and detected accumulation of viral DNA that failed to migrate out of the sample wells of the gels. This DNA lacked terminal restriction fragments and could be converted to unit-length linear species by γ-irradiation, demonstrating the circularization of viral DNA following infection.</abstract><note type="content">Section title: Short Communications</note><relatedItem type="host"><titleInfo><title>Virology</title></titleInfo><titleInfo type="abbreviated"><title>YVIRO</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1993</dateIssued></originInfo><identifier type="ISSN">0042-6822</identifier><identifier type="PII">S0042-6822(00)X0180-8</identifier><part><date>1993</date><detail type="volume"><number>197</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue-pages"><start>1</start><end>510</end></extent><extent unit="pages"><start>459</start><end>462</end></extent></part></relatedItem><identifier type="istex">56594F325E3DF7425FE1D616E0F8C07B05F945F3</identifier><identifier type="ark">ark:/67375/6H6-FBV19BHV-4</identifier><identifier type="DOI">10.1006/viro.1993.1612</identifier><identifier type="PII">S0042-6822(83)71612-0</identifier><accessCondition type="use and reproduction" contentType="copyright">©1993 Academic Press</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource><recordOrigin>Academic Press, ©1993</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-FBV19BHV-4/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000C99 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000C99 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:56594F325E3DF7425FE1D616E0F8C07B05F945F3
|texte= Demonstration of Circularization of Herpes Simplex Virus DNA Following infection Using Pulsed Field Gel Electrophoresis
}}
| This area was generated with Dilib version V0.6.33. |  |