Molecular cloning and expression analysis of IFN-β promoter stimulator 1 in Tibetan pigs
Identifieur interne : 000C36 ( Istex/Corpus ); précédent : 000C35; suivant : 000C37Molecular cloning and expression analysis of IFN-β promoter stimulator 1 in Tibetan pigs
Auteurs : Ye Zhao ; Bing Yu ; Xiangbing Mao ; Guoquan Han ; Qian Mao ; Zhiqing Huang ; Daiwen ChenSource :
- Molecular Biology Reports [ 0301-4851 ] ; 2012-06-01.
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Abstract
Abstract: IFN-β promoter stimulator 1 (IPS-1) is an important adaptor protein linking RIG-I/MDA5 to the downstream signaling molecules and plays the pivotal role in type I interferons induction. In this study, we cloned and characterized Tibetan porcine IPS-1, investigated the tissue distribution, compared different messenger RNA expression for IPS-1 between Tibetan and Crossbred (Duroc × Yorkshire × Landrace) pigs (DLY). The Tibetan porcine IPS-1 gene was first cloned from spleen. The entire open reading frame (ORF) of the IPS-1 is 1,575 bp and encodes for 524 amino acid residues, has 1 putative transmembrane domains, with a higher degree of sequence similarity with common pig (99.37%) and cattle (81.23%) than with human (70.20%) or mouse (63.44%). Real-time quantitative PCR analysis indicated that Tibetan porcine IPS-1 mRNA was most abundant in the liver and kidney. The expression of IPS-1 of Tibetan pigs in most tissues was higher than DLY pigs.
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DOI: 10.1007/s11033-012-1531-1
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In this study, we cloned and characterized Tibetan porcine IPS-1, investigated the tissue distribution, compared different messenger RNA expression for IPS-1 between Tibetan and Crossbred (Duroc × Yorkshire × Landrace) pigs (DLY). The Tibetan porcine IPS-1 gene was first cloned from spleen. The entire open reading frame (ORF) of the IPS-1 is 1,575 bp and encodes for 524 amino acid residues, has 1 putative transmembrane domains, with a higher degree of sequence similarity with common pig (99.37%) and cattle (81.23%) than with human (70.20%) or mouse (63.44%). Real-time quantitative PCR analysis indicated that Tibetan porcine IPS-1 mRNA was most abundant in the liver and kidney. 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NumberingStyle="Unnumbered" TocLevels="0"><ArticleID>1531</ArticleID><ArticleDOI>10.1007/s11033-012-1531-1</ArticleDOI><ArticleSequenceNumber>72</ArticleSequenceNumber><ArticleTitle Language="En" OutputMedium="All">Molecular cloning and expression analysis of IFN-β promoter stimulator 1 in Tibetan pigs</ArticleTitle><ArticleFirstPage>7011</ArticleFirstPage><ArticleLastPage>7017</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2012</Year><Month>1</Month><Day>24</Day></RegistrationDate><Received><Year>2011</Year><Month>9</Month><Day>29</Day></Received><Accepted><Year>2012</Year><Month>1</Month><Day>24</Day></Accepted><OnlineDate><Year>2012</Year><Month>2</Month><Day>9</Day></OnlineDate></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer Science+Business Media B.V.</CopyrightHolderName><CopyrightYear>2012</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1 Aff2"><AuthorName DisplayOrder="Western"><GivenName>Ye</GivenName><FamilyName>Zhao</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff2"><AuthorName DisplayOrder="Western"><GivenName>Bing</GivenName><FamilyName>Yu</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>XiangBing</GivenName><FamilyName>Mao</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>GuoQuan</GivenName><FamilyName>Han</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff2"><AuthorName DisplayOrder="Western"><GivenName>Qian</GivenName><FamilyName>Mao</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Zhiqing</GivenName><FamilyName>Huang</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff2" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Daiwen</GivenName><FamilyName>Chen</FamilyName></AuthorName><Contact><Phone>+86-835-2882088</Phone><Fax>+86-835-2882088</Fax><Email>chendwz@sicau.edu.cn</Email></Contact></Author><Affiliation ID="Aff1"><OrgDivision>Institute of Animal Nutrition</OrgDivision><OrgName>Sichuan Agricultural University</OrgName><OrgAddress><City>Ya’an</City><State>Sichuan</State><Postcode>625014</Postcode><Country Code="CN">People’s Republic of China</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education</OrgDivision><OrgName>Sichuan Agricultural University</OrgName><OrgAddress><City>Ya’an</City><State>Sichuan</State><Postcode>625014</Postcode><Country Code="CN">People’s Republic of China</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En" OutputMedium="All"><Heading>Abstract</Heading><Para>IFN-β promoter stimulator 1 (IPS-1) is an important adaptor protein linking RIG-I/MDA5 to the downstream signaling molecules and plays the pivotal role in type I interferons induction. In this study, we cloned and characterized Tibetan porcine IPS-1, investigated the tissue distribution, compared different messenger RNA expression for IPS-1 between Tibetan and Crossbred (Duroc × Yorkshire × Landrace) pigs (DLY). The Tibetan porcine IPS-1 gene was first cloned from spleen. The entire open reading frame (ORF) of the IPS-1 is 1,575 bp and encodes for 524 amino acid residues, has 1 putative transmembrane domains, with a higher degree of sequence similarity with common pig (99.37%) and cattle (81.23%) than with human (70.20%) or mouse (63.44%). Real-time quantitative PCR analysis indicated that Tibetan porcine IPS-1 mRNA was most abundant in the liver and kidney. The expression of IPS-1 of Tibetan pigs in most tissues was higher than DLY pigs.</Para></Abstract><KeywordGroup Language="En" OutputMedium="All"><Heading>Keywords</Heading><Keyword>Tibetan porcine</Keyword><Keyword>IPS-1</Keyword><Keyword>Cloning</Keyword><Keyword>Gene expression</Keyword></KeywordGroup><ArticleNote Type="ESMHint"><Heading>Electronic supplementary material</Heading><SimplePara>The online version of this article (doi:<ExternalRef><RefSource>10.1007/s11033-012-1531-1</RefSource><RefTarget Address="10.1007/s11033-012-1531-1" TargetType="DOI"></RefTarget></ExternalRef>) contains supplementary material, which is available to authorized users.</SimplePara></ArticleNote></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Molecular cloning and expression analysis of IFN-β promoter stimulator 1 in Tibetan pigs</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Molecular cloning and expression analysis of IFN-β promoter stimulator 1 in Tibetan pigs</title></titleInfo><name type="personal"><namePart type="given">Ye</namePart><namePart type="family">Zhao</namePart><affiliation>Institute of Animal Nutrition, Sichuan Agricultural University, 625014, Ya’an, Sichuan, People’s Republic of China</affiliation><affiliation>Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, 625014, Ya’an, Sichuan, People’s Republic of China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Bing</namePart><namePart type="family">Yu</namePart><affiliation>Institute of Animal Nutrition, Sichuan Agricultural University, 625014, Ya’an, Sichuan, People’s Republic of China</affiliation><affiliation>Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, 625014, Ya’an, Sichuan, People’s Republic of China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">XiangBing</namePart><namePart type="family">Mao</namePart><affiliation>Institute of Animal Nutrition, Sichuan Agricultural University, 625014, Ya’an, Sichuan, People’s Republic of China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">GuoQuan</namePart><namePart type="family">Han</namePart><affiliation>Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, 625014, Ya’an, Sichuan, People’s Republic of China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Qian</namePart><namePart type="family">Mao</namePart><affiliation>Institute of Animal Nutrition, Sichuan Agricultural University, 625014, Ya’an, Sichuan, People’s Republic of China</affiliation><affiliation>Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, 625014, Ya’an, Sichuan, People’s Republic of China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Zhiqing</namePart><namePart type="family">Huang</namePart><affiliation>Institute of Animal Nutrition, Sichuan Agricultural University, 625014, Ya’an, Sichuan, People’s Republic of China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Daiwen</namePart><namePart type="family">Chen</namePart><affiliation>Institute of Animal Nutrition, Sichuan Agricultural University, 625014, Ya’an, Sichuan, People’s Republic of China</affiliation><affiliation>Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, 625014, Ya’an, Sichuan, People’s Republic of China</affiliation><affiliation>E-mail: chendwz@sicau.edu.cn</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer Netherlands</publisher><place><placeTerm type="text">Dordrecht</placeTerm></place><dateCreated encoding="w3cdtf">2011-09-29</dateCreated><dateIssued encoding="w3cdtf">2012-06-01</dateIssued><copyrightDate encoding="w3cdtf">2012</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: IFN-β promoter stimulator 1 (IPS-1) is an important adaptor protein linking RIG-I/MDA5 to the downstream signaling molecules and plays the pivotal role in type I interferons induction. In this study, we cloned and characterized Tibetan porcine IPS-1, investigated the tissue distribution, compared different messenger RNA expression for IPS-1 between Tibetan and Crossbred (Duroc × Yorkshire × Landrace) pigs (DLY). The Tibetan porcine IPS-1 gene was first cloned from spleen. The entire open reading frame (ORF) of the IPS-1 is 1,575 bp and encodes for 524 amino acid residues, has 1 putative transmembrane domains, with a higher degree of sequence similarity with common pig (99.37%) and cattle (81.23%) than with human (70.20%) or mouse (63.44%). Real-time quantitative PCR analysis indicated that Tibetan porcine IPS-1 mRNA was most abundant in the liver and kidney. The expression of IPS-1 of Tibetan pigs in most tissues was higher than DLY pigs.</abstract><subject lang="en"><genre>Keywords</genre><topic>Tibetan porcine</topic><topic>IPS-1</topic><topic>Cloning</topic><topic>Gene expression</topic></subject><relatedItem type="host"><titleInfo><title>Molecular Biology Reports</title><subTitle>An International Journal on Molecular and Cellular Biology</subTitle></titleInfo><titleInfo type="abbreviated"><title>Mol Biol Rep</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">2012-04-24</dateIssued><copyrightDate encoding="w3cdtf">2012</copyrightDate></originInfo><subject><genre>Life Sciences</genre><topic>Animal Anatomy / Morphology / Histology</topic><topic>Animal Biochemistry</topic></subject><identifier type="ISSN">0301-4851</identifier><identifier type="eISSN">1573-4978</identifier><identifier type="JournalID">11033</identifier><identifier type="IssueArticleCount">95</identifier><identifier type="VolumeIssueCount">12</identifier><part><date>2012</date><detail type="volume"><number>39</number><caption>vol.</caption></detail><detail type="issue"><number>6</number><caption>no.</caption></detail><extent unit="pages"><start>7011</start><end>7017</end></extent></part><recordInfo><recordOrigin>Springer Science+Business Media B.V., 2012</recordOrigin></recordInfo></relatedItem><identifier type="istex">089C04F89D6770045DB3039716724A74E6F82346</identifier><identifier type="ark">ark:/67375/VQC-5HHSBV3B-W</identifier><identifier type="DOI">10.1007/s11033-012-1531-1</identifier><identifier type="ArticleID">1531</identifier><identifier type="ArticleID">s11033-012-1531-1</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer Science+Business Media B.V., 2012</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Springer Science+Business Media B.V., 2012</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-5HHSBV3B-W/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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