Molecular cloning and expression analysis of IFN-β promoter stimulator 1 in Tibetan pigs
Identifieur interne : 000C36 ( Istex/Corpus ); précédent : 000C35; suivant : 000C37Molecular cloning and expression analysis of IFN-β promoter stimulator 1 in Tibetan pigs
Auteurs : Ye Zhao ; Bing Yu ; Xiangbing Mao ; Guoquan Han ; Qian Mao ; Zhiqing Huang ; Daiwen ChenSource :
- Molecular Biology Reports [ 0301-4851 ] ; 2012-06-01.
English descriptors
- KwdEn :
Abstract
Abstract: IFN-β promoter stimulator 1 (IPS-1) is an important adaptor protein linking RIG-I/MDA5 to the downstream signaling molecules and plays the pivotal role in type I interferons induction. In this study, we cloned and characterized Tibetan porcine IPS-1, investigated the tissue distribution, compared different messenger RNA expression for IPS-1 between Tibetan and Crossbred (Duroc × Yorkshire × Landrace) pigs (DLY). The Tibetan porcine IPS-1 gene was first cloned from spleen. The entire open reading frame (ORF) of the IPS-1 is 1,575 bp and encodes for 524 amino acid residues, has 1 putative transmembrane domains, with a higher degree of sequence similarity with common pig (99.37%) and cattle (81.23%) than with human (70.20%) or mouse (63.44%). Real-time quantitative PCR analysis indicated that Tibetan porcine IPS-1 mRNA was most abundant in the liver and kidney. The expression of IPS-1 of Tibetan pigs in most tissues was higher than DLY pigs.
Url:
DOI: 10.1007/s11033-012-1531-1
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<front><div type="abstract" xml:lang="en">Abstract: IFN-β promoter stimulator 1 (IPS-1) is an important adaptor protein linking RIG-I/MDA5 to the downstream signaling molecules and plays the pivotal role in type I interferons induction. In this study, we cloned and characterized Tibetan porcine IPS-1, investigated the tissue distribution, compared different messenger RNA expression for IPS-1 between Tibetan and Crossbred (Duroc × Yorkshire × Landrace) pigs (DLY). The Tibetan porcine IPS-1 gene was first cloned from spleen. The entire open reading frame (ORF) of the IPS-1 is 1,575 bp and encodes for 524 amino acid residues, has 1 putative transmembrane domains, with a higher degree of sequence similarity with common pig (99.37%) and cattle (81.23%) than with human (70.20%) or mouse (63.44%). Real-time quantitative PCR analysis indicated that Tibetan porcine IPS-1 mRNA was most abundant in the liver and kidney. The expression of IPS-1 of Tibetan pigs in most tissues was higher than DLY pigs.</div>
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<ArticleTitle Language="En" OutputMedium="All">Molecular cloning and expression analysis of IFN-β promoter stimulator 1 in Tibetan pigs</ArticleTitle>
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<ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1 Aff2"><AuthorName DisplayOrder="Western"><GivenName>Ye</GivenName>
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<FamilyName>Huang</FamilyName>
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<Author AffiliationIDS="Aff1 Aff2" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Daiwen</GivenName>
<FamilyName>Chen</FamilyName>
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<Contact><Phone>+86-835-2882088</Phone>
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<Email>chendwz@sicau.edu.cn</Email>
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<Affiliation ID="Aff1"><OrgDivision>Institute of Animal Nutrition</OrgDivision>
<OrgName>Sichuan Agricultural University</OrgName>
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<Country Code="CN">People’s Republic of China</Country>
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<Para>IFN-β promoter stimulator 1 (IPS-1) is an important adaptor protein linking RIG-I/MDA5 to the downstream signaling molecules and plays the pivotal role in type I interferons induction. In this study, we cloned and characterized Tibetan porcine IPS-1, investigated the tissue distribution, compared different messenger RNA expression for IPS-1 between Tibetan and Crossbred (Duroc × Yorkshire × Landrace) pigs (DLY). The Tibetan porcine IPS-1 gene was first cloned from spleen. The entire open reading frame (ORF) of the IPS-1 is 1,575 bp and encodes for 524 amino acid residues, has 1 putative transmembrane domains, with a higher degree of sequence similarity with common pig (99.37%) and cattle (81.23%) than with human (70.20%) or mouse (63.44%). Real-time quantitative PCR analysis indicated that Tibetan porcine IPS-1 mRNA was most abundant in the liver and kidney. The expression of IPS-1 of Tibetan pigs in most tissues was higher than DLY pigs.</Para>
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<Keyword>Tibetan porcine</Keyword>
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<Keyword>Cloning</Keyword>
<Keyword>Gene expression</Keyword>
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<mods version="3.6"><titleInfo lang="en"><title>Molecular cloning and expression analysis of IFN-β promoter stimulator 1 in Tibetan pigs</title>
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<affiliation>Institute of Animal Nutrition, Sichuan Agricultural University, 625014, Ya’an, Sichuan, People’s Republic of China</affiliation>
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<name type="personal"><namePart type="given">Bing</namePart>
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<name type="personal"><namePart type="given">GuoQuan</namePart>
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<name type="personal"><namePart type="given">Qian</namePart>
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<affiliation>Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, 625014, Ya’an, Sichuan, People’s Republic of China</affiliation>
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<name type="personal"><namePart type="given">Zhiqing</namePart>
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<abstract lang="en">Abstract: IFN-β promoter stimulator 1 (IPS-1) is an important adaptor protein linking RIG-I/MDA5 to the downstream signaling molecules and plays the pivotal role in type I interferons induction. In this study, we cloned and characterized Tibetan porcine IPS-1, investigated the tissue distribution, compared different messenger RNA expression for IPS-1 between Tibetan and Crossbred (Duroc × Yorkshire × Landrace) pigs (DLY). The Tibetan porcine IPS-1 gene was first cloned from spleen. The entire open reading frame (ORF) of the IPS-1 is 1,575 bp and encodes for 524 amino acid residues, has 1 putative transmembrane domains, with a higher degree of sequence similarity with common pig (99.37%) and cattle (81.23%) than with human (70.20%) or mouse (63.44%). Real-time quantitative PCR analysis indicated that Tibetan porcine IPS-1 mRNA was most abundant in the liver and kidney. The expression of IPS-1 of Tibetan pigs in most tissues was higher than DLY pigs.</abstract>
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