Identification of Spodoptera Litura Multicapsid Nucleopolyhedrovirus ORF97, A Novel Protein Associated with Envelope of Occlusion-derived Virus
Identifieur interne : 000966 ( Istex/Corpus ); précédent : 000965; suivant : 000967Identification of Spodoptera Litura Multicapsid Nucleopolyhedrovirus ORF97, A Novel Protein Associated with Envelope of Occlusion-derived Virus
Auteurs : Weichun Chen ; Zhaofei Li ; Sainan Li ; Lingling Li ; Kai Yang ; Yi PangSource :
- Virus Genes [ 0920-8569 ] ; 2006-02-01.
English descriptors
- KwdEn :
Abstract
Abstract: Open reading frame (ORF) 97 of Spodoptera litura multicapsid nucleopolyhedrovirus (Splt97) is a member of 11k gene family (InterPro database accession number: IPR009313) in insect viruses, potentially encoding 112 amino acids with a predicted molecular weight of 13.2 kDa. Sequence analysis indicated that Splt97 has 8–50% amino acid identity with the homologues of other known baculoviruses including Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) ORF108. Splt97 transcription in SpltMNPV-infected Spli-221 cells was detected from 6 to 96 h postinfection (p.i.) and the mRNA start site was mapped within a consensus baculovirus early promoter motif (ACATT). Time course of Splt97 expression in SpltMNPV-infected cells showed that Splt97 was expressed from 48 through 96 h p.i. as a 13 kDa protein. Western blot analysis revealed that Splt97 was present in occlusion-derived virus (ODV). Furthermore, when ODV was fractionated into nucleocapsid and envelope components, Splt97 was shown to be present only in the envelope. These results suggested that Splt97 was a baculovirus ODV-associated protein.
Url:
DOI: 10.1007/s11262-005-5848-x
Links to Exploration step
ISTEX:E911D8CD2D34DFF939706E8821FC5805F4998DF4Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification of Spodoptera Litura Multicapsid Nucleopolyhedrovirus ORF97, A Novel Protein Associated with Envelope of Occlusion-derived Virus</title><author><name sortKey="Chen, Weichun" sort="Chen, Weichun" uniqKey="Chen W" first="Weichun" last="Chen">Weichun Chen</name><affiliation><mods:affiliation>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</mods:affiliation></affiliation></author><author><name sortKey="Li, Zhaofei" sort="Li, Zhaofei" uniqKey="Li Z" first="Zhaofei" last="Li">Zhaofei Li</name><affiliation><mods:affiliation>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</mods:affiliation></affiliation></author><author><name sortKey="Li, Sainan" sort="Li, Sainan" uniqKey="Li S" first="Sainan" last="Li">Sainan Li</name><affiliation><mods:affiliation>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</mods:affiliation></affiliation></author><author><name sortKey="Li, Lingling" sort="Li, Lingling" uniqKey="Li L" first="Lingling" last="Li">Lingling Li</name><affiliation><mods:affiliation>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</mods:affiliation></affiliation></author><author><name sortKey="Yang, Kai" sort="Yang, Kai" uniqKey="Yang K" first="Kai" last="Yang">Kai Yang</name><affiliation><mods:affiliation>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</mods:affiliation></affiliation></author><author><name sortKey="Pang, Yi" sort="Pang, Yi" uniqKey="Pang Y" first="Yi" last="Pang">Yi Pang</name><affiliation><mods:affiliation>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: ls12@zsu.edu.cn</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:E911D8CD2D34DFF939706E8821FC5805F4998DF4</idno><date when="2006" year="2006">2006</date><idno type="doi">10.1007/s11262-005-5848-x</idno><idno type="url">https://api.istex.fr/ark:/67375/VQC-HLSQF612-3/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000966</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000966</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Identification of Spodoptera Litura Multicapsid Nucleopolyhedrovirus ORF97, A Novel Protein Associated with Envelope of Occlusion-derived Virus</title><author><name sortKey="Chen, Weichun" sort="Chen, Weichun" uniqKey="Chen W" first="Weichun" last="Chen">Weichun Chen</name><affiliation><mods:affiliation>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</mods:affiliation></affiliation></author><author><name sortKey="Li, Zhaofei" sort="Li, Zhaofei" uniqKey="Li Z" first="Zhaofei" last="Li">Zhaofei Li</name><affiliation><mods:affiliation>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</mods:affiliation></affiliation></author><author><name sortKey="Li, Sainan" sort="Li, Sainan" uniqKey="Li S" first="Sainan" last="Li">Sainan Li</name><affiliation><mods:affiliation>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</mods:affiliation></affiliation></author><author><name sortKey="Li, Lingling" sort="Li, Lingling" uniqKey="Li L" first="Lingling" last="Li">Lingling Li</name><affiliation><mods:affiliation>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</mods:affiliation></affiliation></author><author><name sortKey="Yang, Kai" sort="Yang, Kai" uniqKey="Yang K" first="Kai" last="Yang">Kai Yang</name><affiliation><mods:affiliation>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</mods:affiliation></affiliation></author><author><name sortKey="Pang, Yi" sort="Pang, Yi" uniqKey="Pang Y" first="Yi" last="Pang">Yi Pang</name><affiliation><mods:affiliation>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: ls12@zsu.edu.cn</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Virus Genes</title><title level="j" type="abbrev">Virus Genes</title><idno type="ISSN">0920-8569</idno><idno type="eISSN">1572-994X</idno><imprint><publisher>Kluwer Academic Publishers</publisher><pubPlace>Boston</pubPlace><date type="published" when="2006-02-01">2006-02-01</date><biblScope unit="volume">32</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="79">79</biblScope><biblScope unit="page" to="84">84</biblScope></imprint><idno type="ISSN">0920-8569</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0920-8569</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Baculovirus</term><term>ORF97</term><term>Occlusion-derived virus</term><term>Spodoptera litura</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Open reading frame (ORF) 97 of Spodoptera litura multicapsid nucleopolyhedrovirus (Splt97) is a member of 11k gene family (InterPro database accession number: IPR009313) in insect viruses, potentially encoding 112 amino acids with a predicted molecular weight of 13.2 kDa. Sequence analysis indicated that Splt97 has 8–50% amino acid identity with the homologues of other known baculoviruses including Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) ORF108. Splt97 transcription in SpltMNPV-infected Spli-221 cells was detected from 6 to 96 h postinfection (p.i.) and the mRNA start site was mapped within a consensus baculovirus early promoter motif (ACATT). Time course of Splt97 expression in SpltMNPV-infected cells showed that Splt97 was expressed from 48 through 96 h p.i. as a 13 kDa protein. Western blot analysis revealed that Splt97 was present in occlusion-derived virus (ODV). Furthermore, when ODV was fractionated into nucleocapsid and envelope components, Splt97 was shown to be present only in the envelope. These results suggested that Splt97 was a baculovirus ODV-associated protein.</div></front></TEI><istex><corpusName>springer-journals</corpusName><author><json:item><name>Weichun Chen</name><affiliations><json:string>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</json:string></affiliations></json:item><json:item><name>Zhaofei Li</name><affiliations><json:string>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</json:string></affiliations></json:item><json:item><name>Sainan Li</name><affiliations><json:string>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</json:string></affiliations></json:item><json:item><name>Lingling Li</name><affiliations><json:string>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</json:string></affiliations></json:item><json:item><name>Kai Yang</name><affiliations><json:string>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</json:string></affiliations></json:item><json:item><name>Yi Pang</name><affiliations><json:string>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</json:string><json:string>E-mail: ls12@zsu.edu.cn</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Spodoptera litura</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Baculovirus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Occlusion-derived virus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>ORF97</value></json:item></subject><articleId><json:string>5848</json:string><json:string>NO00005848</json:string></articleId><arkIstex>ark:/67375/VQC-HLSQF612-3</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Abstract: Open reading frame (ORF) 97 of Spodoptera litura multicapsid nucleopolyhedrovirus (Splt97) is a member of 11k gene family (InterPro database accession number: IPR009313) in insect viruses, potentially encoding 112 amino acids with a predicted molecular weight of 13.2 kDa. Sequence analysis indicated that Splt97 has 8–50% amino acid identity with the homologues of other known baculoviruses including Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) ORF108. Splt97 transcription in SpltMNPV-infected Spli-221 cells was detected from 6 to 96 h postinfection (p.i.) and the mRNA start site was mapped within a consensus baculovirus early promoter motif (ACATT). Time course of Splt97 expression in SpltMNPV-infected cells showed that Splt97 was expressed from 48 through 96 h p.i. as a 13 kDa protein. Western blot analysis revealed that Splt97 was present in occlusion-derived virus (ODV). Furthermore, when ODV was fractionated into nucleocapsid and envelope components, Splt97 was shown to be present only in the envelope. These results suggested that Splt97 was a baculovirus ODV-associated protein.</abstract><qualityIndicators><refBibsNative>false</refBibsNative><abstractWordCount>156</abstractWordCount><abstractCharCount>1128</abstractCharCount><keywordCount>4</keywordCount><score>6.585</score><pdfWordCount>2713</pdfWordCount><pdfCharCount>16778</pdfCharCount><pdfVersion>1.4</pdfVersion><pdfPageCount>6</pdfPageCount><pdfPageSize>595.276 x 785.197 pts</pdfPageSize></qualityIndicators><title>Identification of Spodoptera Litura Multicapsid Nucleopolyhedrovirus ORF97, A Novel Protein Associated with Envelope of Occlusion-derived Virus</title><genre><json:string>research-article</json:string></genre><host><title>Virus Genes</title><language><json:string>unknown</json:string></language><publicationDate>2006</publicationDate><copyrightDate>2006</copyrightDate><issn><json:string>0920-8569</json:string></issn><eissn><json:string>1572-994X</json:string></eissn><journalId><json:string>11262</json:string></journalId><volume>32</volume><issue>1</issue><pages><first>79</first><last>84</last></pages><genre><json:string>journal</json:string></genre><subject><json:item><value>Medical Microbiology</value></json:item><json:item><value>Virology</value></json:item><json:item><value>Plant Sciences</value></json:item></subject></host><ark><json:string>ark:/67375/VQC-HLSQF612-3</json:string></ark><publicationDate>2006</publicationDate><copyrightDate>2006</copyrightDate><doi><json:string>10.1007/s11262-005-5848-x</json:string></doi><id>E911D8CD2D34DFF939706E8821FC5805F4998DF4</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-HLSQF612-3/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-HLSQF612-3/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/VQC-HLSQF612-3/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Identification of Spodoptera Litura Multicapsid Nucleopolyhedrovirus ORF97, A Novel Protein Associated with Envelope of Occlusion-derived Virus</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">Kluwer Academic Publishers</publisher><pubPlace>Boston</pubPlace><availability><licence><p>Springer Science+Business Media, Inc., 2006</p></licence><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</p></availability><date>2005-03-21</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note>Original Article</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Identification of Spodoptera Litura Multicapsid Nucleopolyhedrovirus ORF97, A Novel Protein Associated with Envelope of Occlusion-derived Virus</title><author xml:id="author-0000"><persName><forename type="first">Weichun</forename><surname>Chen</surname></persName><affiliation>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Zhaofei</forename><surname>Li</surname></persName><affiliation>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Sainan</forename><surname>Li</surname></persName><affiliation>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Lingling</forename><surname>Li</surname></persName><affiliation>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</affiliation></author><author xml:id="author-0004"><persName><forename type="first">Kai</forename><surname>Yang</surname></persName><affiliation>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</affiliation></author><author xml:id="author-0005" corresp="yes"><persName><forename type="first">Yi</forename><surname>Pang</surname></persName><email>ls12@zsu.edu.cn</email><affiliation>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</affiliation></author><idno type="istex">E911D8CD2D34DFF939706E8821FC5805F4998DF4</idno><idno type="ark">ark:/67375/VQC-HLSQF612-3</idno><idno type="DOI">10.1007/s11262-005-5848-x</idno><idno type="article-id">5848</idno><idno type="article-id">NO00005848</idno></analytic><monogr><title level="j">Virus Genes</title><title level="j" type="abbrev">Virus Genes</title><idno type="pISSN">0920-8569</idno><idno type="eISSN">1572-994X</idno><idno type="journal-ID">true</idno><idno type="journal-SPIN">VIRU</idno><idno type="volume-issue-count">6</idno><imprint><publisher>Kluwer Academic Publishers</publisher><pubPlace>Boston</pubPlace><date type="published" when="2006-02-01"></date><biblScope unit="volume">32</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="79">79</biblScope><biblScope unit="page" to="84">84</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2005-03-21</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Open reading frame (ORF) 97 of Spodoptera litura multicapsid nucleopolyhedrovirus (Splt97) is a member of 11k gene family (InterPro database accession number: IPR009313) in insect viruses, potentially encoding 112 amino acids with a predicted molecular weight of 13.2 kDa. Sequence analysis indicated that Splt97 has 8–50% amino acid identity with the homologues of other known baculoviruses including Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) ORF108. Splt97 transcription in SpltMNPV-infected Spli-221 cells was detected from 6 to 96 h postinfection (p.i.) and the mRNA start site was mapped within a consensus baculovirus early promoter motif (ACATT). Time course of Splt97 expression in SpltMNPV-infected cells showed that Splt97 was expressed from 48 through 96 h p.i. as a 13 kDa protein. Western blot analysis revealed that Splt97 was present in occlusion-derived virus (ODV). Furthermore, when ODV was fractionated into nucleocapsid and envelope components, Splt97 was shown to be present only in the envelope. These results suggested that Splt97 was a baculovirus ODV-associated protein.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Spodoptera litura</term></item><item><term>Baculovirus</term></item><item><term>Occlusion-derived virus</term></item><item><term>ORF97</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>Biomedicine</head><item><term>Medical Microbiology</term></item><item><term>Virology</term></item><item><term>Plant Sciences</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2005-03-21">Created</change><change when="2006-02-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-HLSQF612-3/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus springer-journals not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" name="istex:docType"></istex:docType><istex:document><Publisher><PublisherInfo><PublisherName>Kluwer Academic Publishers</PublisherName><PublisherLocation>Boston</PublisherLocation></PublisherInfo><Journal><JournalInfo JournalProductType="ArchiveJournal" NumberingStyle="Unnumbered"><JournalID>11262</JournalID><JournalPrintISSN>0920-8569</JournalPrintISSN><JournalElectronicISSN>1572-994X</JournalElectronicISSN><JournalSPIN>VIRU</JournalSPIN><JournalTitle>Virus Genes</JournalTitle><JournalAbbreviatedTitle>Virus Genes</JournalAbbreviatedTitle><JournalSubjectGroup><JournalSubject Type="Primary">Biomedicine</JournalSubject><JournalSubject Type="Secondary">Medical Microbiology</JournalSubject><JournalSubject Type="Secondary">Virology</JournalSubject><JournalSubject Type="Secondary">Plant Sciences</JournalSubject></JournalSubjectGroup></JournalInfo><Volume><VolumeInfo TocLevels="0" VolumeType="Regular"><VolumeIDStart>32</VolumeIDStart><VolumeIDEnd>32</VolumeIDEnd><VolumeIssueCount>6</VolumeIssueCount></VolumeInfo><Issue IssueType="Regular"><IssueInfo TocLevels="0"><IssueIDStart>1</IssueIDStart><IssueIDEnd>1</IssueIDEnd><IssueHistory><CoverDate><Year>2006</Year><Month>02</Month></CoverDate></IssueHistory><IssueCopyright><CopyrightHolderName>Springer Science+Business Media, Inc.</CopyrightHolderName><CopyrightYear>2006</CopyrightYear></IssueCopyright></IssueInfo><Article ID="NO00005848"><ArticleInfo ArticleType="OriginalPaper" ContainsESM="No" Language="En" NumberingStyle="Unnumbered" TocLevels="0"><ArticleID>5848</ArticleID><ArticleDOI>10.1007/s11262-005-5848-x</ArticleDOI><ArticleSequenceNumber>10</ArticleSequenceNumber><ArticleTitle Language="En">Identification of <Emphasis Type="Italic">Spodoptera Litura</Emphasis> Multicapsid Nucleopolyhedrovirus ORF97, A Novel Protein Associated with Envelope of Occlusion-derived Virus</ArticleTitle><ArticleCategory>Original Article</ArticleCategory><ArticleFirstPage>79</ArticleFirstPage><ArticleLastPage>84</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2005</Year><Month>6</Month><Day>21</Day></RegistrationDate><Received><Year>2005</Year><Month>3</Month><Day>21</Day></Received><Revised><Year>2005</Year><Month>6</Month><Day>21</Day></Revised><Accepted><Year>2005</Year><Month>6</Month><Day>21</Day></Accepted></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer Science+Business Media, Inc.</CopyrightHolderName><CopyrightYear>2006</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Weichun</GivenName><FamilyName>Chen</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Zhaofei</GivenName><FamilyName>Li</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Sainan</GivenName><FamilyName>Li</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Lingling</GivenName><FamilyName>Li</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Kai</GivenName><FamilyName>Yang</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Yi</GivenName><FamilyName>Pang</FamilyName></AuthorName><Contact><Email>ls12@zsu.edu.cn</Email></Contact></Author><Affiliation ID="Aff1"><OrgDivision>State Key Laboratory for Biocontrol and Institute of Entomology</OrgDivision><OrgName>Zhongshan University</OrgName><OrgAddress><Postcode>510275</Postcode><City>Guangzhou</City><Country Code="CN">People’s Republic of China</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading><Para>Open reading frame (ORF) 97 of <Emphasis Type="Italic">Spodoptera</Emphasis> <Emphasis Type="Italic">litura</Emphasis> multicapsid nucleopolyhedrovirus (<Emphasis Type="Italic">Splt97</Emphasis>) is a member of 11k gene family (InterPro database accession number: IPR009313) in insect viruses, potentially encoding 112 amino acids with a predicted molecular weight of 13.2 kDa. Sequence analysis indicated that Splt97 has 8–50% amino acid identity with the homologues of other known baculoviruses including <Emphasis Type="Italic">Autographa californica</Emphasis> multicapsid nucleopolyhedrovirus (AcMNPV) ORF108. <Emphasis Type="Italic">Splt97</Emphasis> transcription in SpltMNPV-infected <Emphasis Type="Italic">Spli</Emphasis>-221 cells was detected from 6 to 96 h postinfection (p.i.) and the mRNA start site was mapped within a consensus baculovirus early promoter motif (ACATT). Time course of Splt97 expression in SpltMNPV-infected cells showed that <Emphasis Type="Italic">Splt97</Emphasis> was expressed from 48 through 96 h p.i. as a 13 kDa protein. Western blot analysis revealed that Splt97 was present in occlusion-derived virus (ODV). Furthermore, when ODV was fractionated into nucleocapsid and envelope components, Splt97 was shown to be present only in the envelope. These results suggested that Splt97 was a baculovirus ODV-associated protein.</Para></Abstract><KeywordGroup Language="En"><Heading>Keywords</Heading><Keyword><Emphasis Type="Italic">Spodoptera litura</Emphasis></Keyword><Keyword>Baculovirus</Keyword><Keyword>Occlusion-derived virus</Keyword><Keyword>ORF97</Keyword></KeywordGroup><ArticleNote Type="Misc"><SimplePara>These authors contributed equally to the work.</SimplePara></ArticleNote></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Identification of Spodoptera Litura Multicapsid Nucleopolyhedrovirus ORF97, A Novel Protein Associated with Envelope of Occlusion-derived Virus</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Identification of Spodoptera Litura Multicapsid Nucleopolyhedrovirus ORF97, A Novel Protein Associated with Envelope of Occlusion-derived Virus</title></titleInfo><name type="personal"><namePart type="given">Weichun</namePart><namePart type="family">Chen</namePart><affiliation>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Zhaofei</namePart><namePart type="family">Li</namePart><affiliation>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Sainan</namePart><namePart type="family">Li</namePart><affiliation>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Lingling</namePart><namePart type="family">Li</namePart><affiliation>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Kai</namePart><namePart type="family">Yang</namePart><affiliation>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Yi</namePart><namePart type="family">Pang</namePart><affiliation>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People’s Republic of China</affiliation><affiliation>E-mail: ls12@zsu.edu.cn</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Kluwer Academic Publishers</publisher><place><placeTerm type="text">Boston</placeTerm></place><dateCreated encoding="w3cdtf">2005-03-21</dateCreated><dateIssued encoding="w3cdtf">2006-02-01</dateIssued><copyrightDate encoding="w3cdtf">2006</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: Open reading frame (ORF) 97 of Spodoptera litura multicapsid nucleopolyhedrovirus (Splt97) is a member of 11k gene family (InterPro database accession number: IPR009313) in insect viruses, potentially encoding 112 amino acids with a predicted molecular weight of 13.2 kDa. Sequence analysis indicated that Splt97 has 8–50% amino acid identity with the homologues of other known baculoviruses including Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) ORF108. Splt97 transcription in SpltMNPV-infected Spli-221 cells was detected from 6 to 96 h postinfection (p.i.) and the mRNA start site was mapped within a consensus baculovirus early promoter motif (ACATT). Time course of Splt97 expression in SpltMNPV-infected cells showed that Splt97 was expressed from 48 through 96 h p.i. as a 13 kDa protein. Western blot analysis revealed that Splt97 was present in occlusion-derived virus (ODV). Furthermore, when ODV was fractionated into nucleocapsid and envelope components, Splt97 was shown to be present only in the envelope. These results suggested that Splt97 was a baculovirus ODV-associated protein.</abstract><note>Original Article</note><subject lang="en"><genre>Keywords</genre><topic>Spodoptera litura</topic><topic>Baculovirus</topic><topic>Occlusion-derived virus</topic><topic>ORF97</topic></subject><relatedItem type="host"><titleInfo><title>Virus Genes</title></titleInfo><titleInfo type="abbreviated"><title>Virus Genes</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">2006-02-01</dateIssued><copyrightDate encoding="w3cdtf">2006</copyrightDate></originInfo><subject><genre>Biomedicine</genre><topic>Medical Microbiology</topic><topic>Virology</topic><topic>Plant Sciences</topic></subject><identifier type="ISSN">0920-8569</identifier><identifier type="eISSN">1572-994X</identifier><identifier type="JournalID">11262</identifier><identifier type="JournalSPIN">VIRU</identifier><identifier type="VolumeIssueCount">6</identifier><part><date>2006</date><detail type="volume"><number>32</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="pages"><start>79</start><end>84</end></extent></part><recordInfo><recordOrigin>Springer Science+Business Media, Inc., 2006</recordOrigin></recordInfo></relatedItem><identifier type="istex">E911D8CD2D34DFF939706E8821FC5805F4998DF4</identifier><identifier type="ark">ark:/67375/VQC-HLSQF612-3</identifier><identifier type="DOI">10.1007/s11262-005-5848-x</identifier><identifier type="ArticleID">5848</identifier><identifier type="ArticleID">NO00005848</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer Science+Business Media, Inc., 2006</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Springer Science+Business Media, Inc., 2006</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-HLSQF612-3/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000966 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000966 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:E911D8CD2D34DFF939706E8821FC5805F4998DF4
|texte= Identification of Spodoptera Litura Multicapsid Nucleopolyhedrovirus ORF97, A Novel Protein Associated with Envelope of Occlusion-derived Virus
}}
| This area was generated with Dilib version V0.6.33. |  |