Measurement of Noncovalent Complexes with High m/z by Electrospray Time-of-Flight Mass Spectrometry
Identifieur interne : 000903 ( Istex/Corpus ); précédent : 000902; suivant : 000904Measurement of Noncovalent Complexes with High m/z by Electrospray Time-of-Flight Mass Spectrometry
Auteurs : I. V. Chernushevich ; W. Ens ; K. G. StandingSource :
- NATO ASI Series [ 1389-2185 ]
Abstract
Abstract: In the last few years the electrospray (ES) ionization technique has proved to be gentle enough to obtain information on the higher-order structure of biomolecules [1]. However, most ES-MS studies with proteins have been carried out in acidic solutions. Such acidic solutions, particularly in combination with organic solvents, denature biopolymers, which then acquire sufficient charge to be detected within the limited m/z range of most commercial quadrupole mass spectrometers. These conditions are usually sufficiently severe so as to destroy any higher order structure, which must be examined closer to physiological conditions. There the charge acquired may be much lower, resulting in m/z values beyond the range of normal quadrupole instruments (see for example [2]).
Url:
DOI: 10.1007/978-94-015-9046-4_8
Links to Exploration step
ISTEX:14CDBCD173F9D463E751FEE12F04EB8E35641A68Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Measurement of Noncovalent Complexes with High m/z by Electrospray Time-of-Flight Mass Spectrometry</title><author><name sortKey="Chernushevich, I V" sort="Chernushevich, I V" uniqKey="Chernushevich I" first="I. V." last="Chernushevich">I. V. Chernushevich</name><affiliation><mods:affiliation>Physics Department, University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada</mods:affiliation></affiliation></author><author><name sortKey="Ens, W" sort="Ens, W" uniqKey="Ens W" first="W." last="Ens">W. Ens</name><affiliation><mods:affiliation>Physics Department, University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada</mods:affiliation></affiliation></author><author><name sortKey="Standing, K G" sort="Standing, K G" uniqKey="Standing K" first="K. G." last="Standing">K. G. Standing</name><affiliation><mods:affiliation>Physics Department, University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:14CDBCD173F9D463E751FEE12F04EB8E35641A68</idno><date when="1998" year="1998">1998</date><idno type="doi">10.1007/978-94-015-9046-4_8</idno><idno type="url">https://api.istex.fr/ark:/67375/HCB-RZZ54D6F-F/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000903</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000903</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Measurement of Noncovalent Complexes with High m/z by Electrospray Time-of-Flight Mass Spectrometry</title><author><name sortKey="Chernushevich, I V" sort="Chernushevich, I V" uniqKey="Chernushevich I" first="I. V." last="Chernushevich">I. V. Chernushevich</name><affiliation><mods:affiliation>Physics Department, University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada</mods:affiliation></affiliation></author><author><name sortKey="Ens, W" sort="Ens, W" uniqKey="Ens W" first="W." last="Ens">W. Ens</name><affiliation><mods:affiliation>Physics Department, University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada</mods:affiliation></affiliation></author><author><name sortKey="Standing, K G" sort="Standing, K G" uniqKey="Standing K" first="K. G." last="Standing">K. G. Standing</name><affiliation><mods:affiliation>Physics Department, University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="s" type="main" xml:lang="en">NATO ASI Series</title><idno type="ISSN">1389-2185</idno><idno type="ISSN">1389-2185</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1389-2185</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: In the last few years the electrospray (ES) ionization technique has proved to be gentle enough to obtain information on the higher-order structure of biomolecules [1]. However, most ES-MS studies with proteins have been carried out in acidic solutions. Such acidic solutions, particularly in combination with organic solvents, denature biopolymers, which then acquire sufficient charge to be detected within the limited m/z range of most commercial quadrupole mass spectrometers. These conditions are usually sufficiently severe so as to destroy any higher order structure, which must be examined closer to physiological conditions. There the charge acquired may be much lower, resulting in m/z values beyond the range of normal quadrupole instruments (see for example [2]).</div></front></TEI><istex><corpusName>springer-ebooks</corpusName><author><json:item><name>I. V. Chernushevich</name><affiliations><json:string>Physics Department, University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada</json:string></affiliations></json:item><json:item><name>W. Ens</name><affiliations><json:string>Physics Department, University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada</json:string></affiliations></json:item><json:item><name>K. G. Standing</name><affiliations><json:string>Physics Department, University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada</json:string></affiliations></json:item></author><arkIstex>ark:/67375/HCB-RZZ54D6F-F</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Abstract: In the last few years the electrospray (ES) ionization technique has proved to be gentle enough to obtain information on the higher-order structure of biomolecules [1]. However, most ES-MS studies with proteins have been carried out in acidic solutions. Such acidic solutions, particularly in combination with organic solvents, denature biopolymers, which then acquire sufficient charge to be detected within the limited m/z range of most commercial quadrupole mass spectrometers. These conditions are usually sufficiently severe so as to destroy any higher order structure, which must be examined closer to physiological conditions. There the charge acquired may be much lower, resulting in m/z values beyond the range of normal quadrupole instruments (see for example [2]).</abstract><qualityIndicators><score>6.783</score><pdfWordCount>3403</pdfWordCount><pdfCharCount>21044</pdfCharCount><pdfVersion>1.4</pdfVersion><pdfPageCount>16</pdfPageCount><pdfPageSize>439.37 x 666.142 pts</pdfPageSize><refBibsNative>false</refBibsNative><abstractWordCount>115</abstractWordCount><abstractCharCount>785</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Measurement of Noncovalent Complexes with High m/z by Electrospray Time-of-Flight Mass Spectrometry</title><chapterId><json:string>8</json:string><json:string>Chap8</json:string></chapterId><genre><json:string>research-article</json:string></genre><serie><title>NATO ASI Series</title><language><json:string>unknown</json:string></language><copyrightDate>1998</copyrightDate><issn><json:string>1389-2185</json:string></issn></serie><host><title>New Methods for the Study of Biomolecular Complexes</title><language><json:string>unknown</json:string></language><copyrightDate>1998</copyrightDate><doi><json:string>10.1007/978-94-015-9046-4</json:string></doi><issn><json:string>1389-2185</json:string></issn><eisbn><json:string>978-94-015-9046-4</json:string></eisbn><bookId><json:string>978-94-015-9046-4</json:string></bookId><isbn><json:string>978-90-481-5017-5</json:string></isbn><volume>510</volume><pages><first>101</first><last>116</last></pages><genre><json:string>book-series</json:string></genre><editor><json:item><name>Werner Ens</name><affiliations><json:string>Dept. of Physics, The University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada</json:string><json:string>E-mail: W_Ens@UManitoba.CA</json:string></affiliations></json:item><json:item><name>Kenneth G. Standing</name><affiliations><json:string>Dept. of Physics, The University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada</json:string><json:string>E-mail: standin@cc.umanitoba.ca</json:string></affiliations></json:item><json:item><name>Igor V. Chernushevich</name><affiliations><json:string>Dept. of Physics, The University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada</json:string><json:string>PE SCIEX, Concord, Ontario, Canada</json:string><json:string>E-mail: chernus@cc.umanitoba.ca</json:string></affiliations></json:item></editor><subject><json:item><value>Physics and Astronomy</value></json:item><json:item><value>Life Sciences</value></json:item><json:item><value>Biochemistry, general</value></json:item><json:item><value>Analytical Chemistry</value></json:item></subject></host><ark><json:string>ark:/67375/HCB-RZZ54D6F-F</json:string></ark><publicationDate>1998</publicationDate><copyrightDate>1998</copyrightDate><doi><json:string>10.1007/978-94-015-9046-4_8</json:string></doi><id>14CDBCD173F9D463E751FEE12F04EB8E35641A68</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/HCB-RZZ54D6F-F/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/HCB-RZZ54D6F-F/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/HCB-RZZ54D6F-F/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Measurement of Noncovalent Complexes with High m/z by Electrospray Time-of-Flight Mass Spectrometry</title></titleStmt><publicationStmt><authority>ISTEX</authority><availability><licence>Springer Science+Business Media Dordrecht</licence></availability><date when="1998">1998</date></publicationStmt><notesStmt><note type="content-type" subtype="research-article" source="OriginalPaper" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="publication-type" subtype="book-series" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0G6R5W5T-Z">book-series</note></notesStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Measurement of Noncovalent Complexes with High m/z by Electrospray Time-of-Flight Mass Spectrometry</title><author><persName><forename type="first">I.</forename><forename type="first">V.</forename><surname>Chernushevich</surname></persName><affiliation><orgName type="department">Physics Department</orgName><orgName type="institution">University of Manitoba</orgName><address><settlement>Winnipeg</settlement><region>Manitoba</region><postCode>R3T 2N2</postCode><country key="CA">CANADA</country></address></affiliation></author><author><persName><forename type="first">W.</forename><surname>Ens</surname></persName><affiliation><orgName type="department">Physics Department</orgName><orgName type="institution">University of Manitoba</orgName><address><settlement>Winnipeg</settlement><region>Manitoba</region><postCode>R3T 2N2</postCode><country key="CA">CANADA</country></address></affiliation></author><author><persName><forename type="first">K.</forename><forename type="first">G.</forename><surname>Standing</surname></persName><affiliation><orgName type="department">Physics Department</orgName><orgName type="institution">University of Manitoba</orgName><address><settlement>Winnipeg</settlement><region>Manitoba</region><postCode>R3T 2N2</postCode><country key="CA">CANADA</country></address></affiliation></author><idno type="istex">14CDBCD173F9D463E751FEE12F04EB8E35641A68</idno><idno type="ark">ark:/67375/HCB-RZZ54D6F-F</idno><idno type="DOI">10.1007/978-94-015-9046-4_8</idno></analytic><monogr><title level="m" type="main">New Methods for the Study of Biomolecular Complexes</title><title level="m" type="part">Electrospray Ionization Mass Spectrometry</title><idno type="DOI">10.1007/978-94-015-9046-4</idno><idno type="book-id">978-94-015-9046-4</idno><idno type="ISBN">978-90-481-5017-5</idno><idno type="eISBN">978-94-015-9046-4</idno><idno type="chapter-id">Chap8</idno><idno type="part-id">Part2</idno><editor><persName><forename type="first">Werner</forename><surname>Ens</surname></persName><email>W_Ens@UManitoba.CA</email><affiliation><orgName type="department">Dept. of Physics</orgName><orgName type="institution">The University of Manitoba</orgName><address><postCode>R3T 2N2</postCode><settlement>Winnipeg</settlement><region>Manitoba</region><country key="CA">CANADA</country></address></affiliation></editor><editor><persName><forename type="first">Kenneth</forename><forename type="first">G.</forename><surname>Standing</surname></persName><email>standin@cc.umanitoba.ca</email><affiliation><orgName type="department">Dept. of Physics</orgName><orgName type="institution">The University of Manitoba</orgName><address><postCode>R3T 2N2</postCode><settlement>Winnipeg</settlement><region>Manitoba</region><country key="CA">CANADA</country></address></affiliation></editor><editor><persName><forename type="first">Igor</forename><forename type="first">V.</forename><surname>Chernushevich</surname></persName><email>chernus@cc.umanitoba.ca</email><affiliation><orgName type="department">Dept. of Physics</orgName><orgName type="institution">The University of Manitoba</orgName><address><postCode>R3T 2N2</postCode><settlement>Winnipeg</settlement><region>Manitoba</region><country key="CA">CANADA</country></address></affiliation><affiliation><orgName type="institution">PE SCIEX</orgName><address><settlement>Concord</settlement><region>Ontario</region><country key="CA">CANADA</country></address></affiliation></editor><imprint><biblScope unit="vol">510</biblScope><biblScope unit="page" from="101">101</biblScope><biblScope unit="page" to="116">116</biblScope><biblScope unit="chapter-count">25</biblScope><biblScope unit="part-chapter-count">8</biblScope></imprint></monogr><series><title level="s" type="main" xml:lang="en">NATO ASI Series</title><idno type="pISSN">1389-2185</idno><idno type="seriesID">5658</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><abstract xml:lang="en"><head>Abstract</head><p>In the last few years the electrospray (ES) ionization technique has proved to be gentle enough to obtain information on the higher-order structure of biomolecules [1]. However, most ES-MS studies with proteins have been carried out in acidic solutions. Such acidic solutions, particularly in combination with organic solvents, denature biopolymers, which then acquire sufficient charge to be detected within the limited <hi rend="italic">m</hi>/<hi rend="italic">z</hi> range of most commercial quadrupole mass spectrometers. These conditions are usually sufficiently severe so as to destroy any higher order structure, which must be examined closer to physiological conditions. There the charge acquired may be much lower, resulting in <hi rend="italic">m</hi>/<hi rend="italic">z</hi> values beyond the range of normal quadrupole instruments (see for example [2]).</p></abstract><textClass ana="subject"><keywords scheme="book-subject-collection"><list><label>SUCO11651</label><item><term>Physics and Astronomy</term></item></list></keywords></textClass><textClass ana="subject"><keywords scheme="book-subject"><list><label>SCL</label><item><term type="Primary">Life Sciences</term></item><label>SCL14005</label><item><term type="Secondary" subtype="priority-1">Biochemistry, general</term></item><label>SCC11006</label><item><term type="Secondary" subtype="priority-2">Analytical Chemistry</term></item></list></keywords></textClass><langUsage><language ident="EN"></language></langUsage></profileDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/HCB-RZZ54D6F-F/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus springer-ebooks not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" name="istex:docType"></istex:docType><istex:document><Publisher><PublisherInfo><PublisherName>Springer Netherlands</PublisherName><PublisherLocation>Dordrecht</PublisherLocation><PublisherImprintName>Springer</PublisherImprintName></PublisherInfo><Series><SeriesInfo SeriesType="Series" TocLevels="0"><SeriesID>5658</SeriesID><SeriesPrintISSN>1389-2185</SeriesPrintISSN><SeriesTitle Language="En">NATO ASI Series</SeriesTitle><SeriesSubTitle Language="En">Series C: Mathematical and Physical Sciences</SeriesSubTitle></SeriesInfo><Book Language="En"><BookInfo BookProductType="Contributed volume" ContainsESM="No" Language="En" MediaType="eBook" NumberingStyle="Unnumbered" OutputMedium="All" TocLevels="0"><BookID>978-94-015-9046-4</BookID><BookTitle>New Methods for the Study of Biomolecular Complexes</BookTitle><BookVolumeNumber>510</BookVolumeNumber><BookSequenceNumber>518</BookSequenceNumber><BookDOI>10.1007/978-94-015-9046-4</BookDOI><BookTitleID>94834</BookTitleID><BookPrintISBN>978-90-481-5017-5</BookPrintISBN><BookElectronicISBN>978-94-015-9046-4</BookElectronicISBN><BookChapterCount>25</BookChapterCount><BookCopyright><CopyrightHolderName>Springer Science+Business Media B.V.</CopyrightHolderName><CopyrightYear>1998</CopyrightYear></BookCopyright><BookSubjectGroup><BookSubject Code="SCL" Type="Primary">Life Sciences</BookSubject><BookSubject Code="SCL14005" Priority="1" Type="Secondary">Biochemistry, general</BookSubject><BookSubject Code="SCC11006" Priority="2" Type="Secondary">Analytical Chemistry</BookSubject><SubjectCollection Code="SUCO11651">Physics and Astronomy</SubjectCollection></BookSubjectGroup><BookContext><SeriesID>5658</SeriesID></BookContext></BookInfo><BookHeader><EditorGroup><Editor AffiliationIDS="Aff1"><EditorName DisplayOrder="Western"><GivenName>Werner</GivenName><FamilyName>Ens</FamilyName></EditorName><Contact><Phone>204 474 6178</Phone><Fax>204 269 8489</Fax><Email>W_Ens@UManitoba.CA</Email></Contact></Editor><Editor AffiliationIDS="Aff1"><EditorName DisplayOrder="Western"><GivenName>Kenneth</GivenName><GivenName>G.</GivenName><FamilyName>Standing</FamilyName></EditorName><Contact><Phone>204 474 9358</Phone><Fax>204 269 8489</Fax><Email>standin@cc.umanitoba.ca</Email></Contact></Editor><Editor AffiliationIDS="Aff1 Aff2"><EditorName DisplayOrder="Western"><GivenName>Igor</GivenName><GivenName>V.</GivenName><FamilyName>Chernushevich</FamilyName></EditorName><Contact><Phone>204 474 6184</Phone><Fax>204 269 8489</Fax><Email>chernus@cc.umanitoba.ca</Email></Contact></Editor><Affiliation ID="Aff1"><OrgDivision>Dept. of Physics</OrgDivision><OrgName>The University of Manitoba</OrgName><OrgAddress><Postcode>R3T 2N2</Postcode><City>Winnipeg</City><State>Manitoba</State><Country>Canada</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgName>PE SCIEX</OrgName><OrgAddress><City>Concord</City><State>Ontario</State><Country>Canada</Country></OrgAddress></Affiliation></EditorGroup></BookHeader><Part ID="Part2"><PartInfo TocLevels="0"><PartID>2</PartID><PartSequenceNumber>2</PartSequenceNumber><PartTitle>Electrospray Ionization Mass Spectrometry</PartTitle><PartChapterCount>8</PartChapterCount><PartContext><SeriesID>5658</SeriesID><BookID>978-94-015-9046-4</BookID><BookTitle>New Methods for the Study of Biomolecular Complexes</BookTitle></PartContext></PartInfo><Chapter ID="Chap8" Language="En"><ChapterInfo ChapterType="OriginalPaper" ContainsESM="No" Language="En" NumberingStyle="Unnumbered" OutputMedium="All" TocLevels="0"><ChapterID>8</ChapterID><ChapterDOI>10.1007/978-94-015-9046-4_8</ChapterDOI><ChapterSequenceNumber>8</ChapterSequenceNumber><ChapterTitle Language="En">Measurement of Noncovalent Complexes with High m/z by Electrospray Time-of-Flight Mass Spectrometry</ChapterTitle><ChapterFirstPage>101</ChapterFirstPage><ChapterLastPage>116</ChapterLastPage><ChapterCopyright><CopyrightHolderName>Springer Science+Business Media Dordrecht</CopyrightHolderName><CopyrightYear>1998</CopyrightYear></ChapterCopyright><ChapterHistory><RegistrationDate><Year>2012</Year><Month>11</Month><Day>17</Day></RegistrationDate></ChapterHistory><ChapterGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ChapterGrants><ChapterContext><SeriesID>5658</SeriesID><PartID>2</PartID><BookID>978-94-015-9046-4</BookID><BookTitle>New Methods for the Study of Biomolecular Complexes</BookTitle></ChapterContext></ChapterInfo><ChapterHeader><AuthorGroup><Author AffiliationIDS="Aff3"><AuthorName DisplayOrder="Western"><GivenName>I.</GivenName><GivenName>V.</GivenName><FamilyName>Chernushevich</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff3"><AuthorName DisplayOrder="Western"><GivenName>W.</GivenName><FamilyName>Ens</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff3"><AuthorName DisplayOrder="Western"><GivenName>K.</GivenName><GivenName>G.</GivenName><FamilyName>Standing</FamilyName></AuthorName></Author><Affiliation ID="Aff3"><OrgDivision>Physics Department</OrgDivision><OrgName>University of Manitoba</OrgName><OrgAddress><City>Winnipeg</City><State>Manitoba</State><Postcode>R3T 2N2</Postcode><Country>Canada</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En" OutputMedium="Online"><Heading>Abstract</Heading><Para>In the last few years the electrospray (ES) ionization technique has proved to be gentle enough to obtain information on the higher-order structure of biomolecules [1]. However, most ES-MS studies with proteins have been carried out in acidic solutions. Such acidic solutions, particularly in combination with organic solvents, denature biopolymers, which then acquire sufficient charge to be detected within the limited <Emphasis Type="Italic">m</Emphasis>/<Emphasis Type="Italic">z</Emphasis> range of most commercial quadrupole mass spectrometers. These conditions are usually sufficiently severe so as to destroy any higher order structure, which must be examined closer to physiological conditions. There the charge acquired may be much lower, resulting in <Emphasis Type="Italic">m</Emphasis>/<Emphasis Type="Italic">z</Emphasis> values beyond the range of normal quadrupole instruments (see for example [2]).</Para></Abstract></ChapterHeader><NoBody></NoBody></Chapter></Part></Book></Series></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Measurement of Noncovalent Complexes with High m/z by Electrospray Time-of-Flight Mass Spectrometry</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Measurement of Noncovalent Complexes with High m/z by Electrospray Time-of-Flight Mass Spectrometry</title></titleInfo><name type="personal"><namePart type="given">I.</namePart><namePart type="given">V.</namePart><namePart type="family">Chernushevich</namePart><affiliation>Physics Department, University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">W.</namePart><namePart type="family">Ens</namePart><affiliation>Physics Department, University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.</namePart><namePart type="given">G.</namePart><namePart type="family">Standing</namePart><affiliation>Physics Department, University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" type="research-article" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer Netherlands</publisher><place><placeTerm type="text">Dordrecht</placeTerm></place><dateIssued encoding="w3cdtf">1998</dateIssued><copyrightDate encoding="w3cdtf">1998</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: In the last few years the electrospray (ES) ionization technique has proved to be gentle enough to obtain information on the higher-order structure of biomolecules [1]. However, most ES-MS studies with proteins have been carried out in acidic solutions. Such acidic solutions, particularly in combination with organic solvents, denature biopolymers, which then acquire sufficient charge to be detected within the limited m/z range of most commercial quadrupole mass spectrometers. These conditions are usually sufficiently severe so as to destroy any higher order structure, which must be examined closer to physiological conditions. There the charge acquired may be much lower, resulting in m/z values beyond the range of normal quadrupole instruments (see for example [2]).</abstract><relatedItem type="host"><titleInfo><title>New Methods for the Study of Biomolecular Complexes</title></titleInfo><name type="personal"><namePart type="given">Werner</namePart><namePart type="family">Ens</namePart><affiliation>Dept. of Physics, The University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada</affiliation><affiliation>E-mail: W_Ens@UManitoba.CA</affiliation><role><roleTerm type="text">editor</roleTerm></role></name><name type="personal"><namePart type="given">Kenneth</namePart><namePart type="given">G.</namePart><namePart type="family">Standing</namePart><affiliation>Dept. of Physics, The University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada</affiliation><affiliation>E-mail: standin@cc.umanitoba.ca</affiliation><role><roleTerm type="text">editor</roleTerm></role></name><name type="personal"><namePart type="given">Igor</namePart><namePart type="given">V.</namePart><namePart type="family">Chernushevich</namePart><affiliation>Dept. of Physics, The University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada</affiliation><affiliation>PE SCIEX, Concord, Ontario, Canada</affiliation><affiliation>E-mail: chernus@cc.umanitoba.ca</affiliation><role><roleTerm type="text">editor</roleTerm></role></name><genre type="book-series" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0G6R5W5T-Z">book-series</genre><originInfo><publisher>Springer</publisher><copyrightDate encoding="w3cdtf">1998</copyrightDate><issuance>monographic</issuance></originInfo><subject><genre>Book-Subject-Collection</genre><topic authority="SpringerSubjectCodes" authorityURI="SUCO11651">Physics and Astronomy</topic></subject><subject><genre>Book-Subject-Group</genre><topic authority="SpringerSubjectCodes" authorityURI="SCL">Life Sciences</topic><topic authority="SpringerSubjectCodes" authorityURI="SCL14005">Biochemistry, general</topic><topic authority="SpringerSubjectCodes" authorityURI="SCC11006">Analytical Chemistry</topic></subject><identifier type="DOI">10.1007/978-94-015-9046-4</identifier><identifier type="ISBN">978-90-481-5017-5</identifier><identifier type="eISBN">978-94-015-9046-4</identifier><identifier type="ISSN">1389-2185</identifier><identifier type="BookTitleID">94834</identifier><identifier type="BookID">978-94-015-9046-4</identifier><identifier type="BookChapterCount">25</identifier><identifier type="BookVolumeNumber">510</identifier><identifier type="BookSequenceNumber">518</identifier><identifier type="PartChapterCount">8</identifier><part><date>1998</date><detail type="part"><title>Electrospray Ionization Mass Spectrometry</title></detail><detail type="volume"><number>510</number><caption>vol.</caption></detail><extent unit="pages"><start>101</start><end>116</end></extent></part><recordInfo><recordOrigin>Springer Science+Business Media B.V., 1998</recordOrigin></recordInfo></relatedItem><relatedItem type="series"><titleInfo><title>NATO ASI Series</title><subTitle>Series C: Mathematical and Physical Sciences</subTitle></titleInfo><originInfo><publisher>Springer</publisher><copyrightDate encoding="w3cdtf">1998</copyrightDate><issuance>serial</issuance></originInfo><identifier type="ISSN">1389-2185</identifier><identifier type="SeriesID">5658</identifier><recordInfo><recordOrigin>Springer Science+Business Media B.V., 1998</recordOrigin></recordInfo></relatedItem><identifier type="istex">14CDBCD173F9D463E751FEE12F04EB8E35641A68</identifier><identifier type="ark">ark:/67375/HCB-RZZ54D6F-F</identifier><identifier type="DOI">10.1007/978-94-015-9046-4_8</identifier><identifier type="ChapterID">8</identifier><identifier type="ChapterID">Chap8</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer Science+Business Media B.V., 1998</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-RLRX46XW-4">springer</recordContentSource><recordOrigin>Springer Science+Business Media Dordrecht, 1998</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/HCB-RZZ54D6F-F/record.json</uri></json:item></metadata><annexes><json:item><extension>xml</extension><original>true</original><mimetype>application/xml</mimetype><uri>https://api.istex.fr/ark:/67375/HCB-RZZ54D6F-F/annexes.xml</uri></json:item></annexes></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000903 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000903 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:14CDBCD173F9D463E751FEE12F04EB8E35641A68
|texte= Measurement of Noncovalent Complexes with High m/z by Electrospray Time-of-Flight Mass Spectrometry
}}
| This area was generated with Dilib version V0.6.33. |  |