Oligomerization of Recombinant Penton Base of Adenovirus Type 2 and Its Assembly with Fiber in Baculovirus-Infected Cells
Identifieur interne : 000826 ( Istex/Corpus ); précédent : 000825; suivant : 000827Oligomerization of Recombinant Penton Base of Adenovirus Type 2 and Its Assembly with Fiber in Baculovirus-Infected Cells
Auteurs : Lucie Karayan ; Bernard Gay ; Jacqueline Gerfaux ; Pierre A. BoulangerSource :
- Virology [ 0042-6822 ] ; 1994.
Abstract
Abstract: Full-length Ad2 penton base (PbFL571) and amino-terminal (PbAT69) and carboxy-terminal deletion mutants (PbCT550 and PbCT531) were expressed at high levels in recombinant baculovirus-infected insect cells. PbFL571, and with a lesser efficiency PbAT69, assembled penton base capsomers (9S pentamers), whereas pentamer assembly was abolished with the deletion of the 21 C-terminal amino acids (as in PbCT550). A discrete population of penton base capsomers in PbFL571 and PbAT69 was found to occur as 12S decamers. PbFL571 and PbAT69 penton base were able to bind to full-length (but not to N-terminal deletion mutants of) fiber trimer to form authentic penton capsomer when coexpressed in trans in double-infected cells. Penton base monomers accumulated by PbCT550 and PbCT531 were capable of interacting with both fiber trimers and monomers. This suggested that mutual binding sites exist in the fiber and penton base subunits, and that the fiber-binding domain is located between residues 70 and 531 in the penton base, with its complementary domain situated at the N-terminus of the fiber. Intranuclear inclusions of recombinant protein were observed with the three deletion mutants PbAT69, PbCT550, and PbCT531, implying the existence of karyophilic signal(s) in the central domain of penton base.
Url:
DOI: 10.1006/viro.1994.1400
Links to Exploration step
ISTEX:14549E3ADBFA9B8B8122B30B17D8993C1B55792FLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Oligomerization of Recombinant Penton Base of Adenovirus Type 2 and Its Assembly with Fiber in Baculovirus-Infected Cells</title><author><name sortKey="Karayan, Lucie" sort="Karayan, Lucie" uniqKey="Karayan L" first="Lucie" last="Karayan">Lucie Karayan</name><affiliation><mods:affiliation>Laboratoire de Virologie et Pathogénèse Moléculaires (CNRS URA 1487), Faculté de Médecine, Boulevard Henri IV, 34060 Montpellier, France</mods:affiliation></affiliation></author><author><name sortKey="Gay, Bernard" sort="Gay, Bernard" uniqKey="Gay B" first="Bernard" last="Gay">Bernard Gay</name><affiliation><mods:affiliation>Laboratoire de Virologie et Pathogénèse Moléculaires (CNRS URA 1487), Faculté de Médecine, Boulevard Henri IV, 34060 Montpellier, France</mods:affiliation></affiliation></author><author><name sortKey="Gerfaux, Jacqueline" sort="Gerfaux, Jacqueline" uniqKey="Gerfaux J" first="Jacqueline" last="Gerfaux">Jacqueline Gerfaux</name><affiliation><mods:affiliation>Laboratoire de Virologie et Pathogénèse Moléculaires (CNRS URA 1487), Faculté de Médecine, Boulevard Henri IV, 34060 Montpellier, France</mods:affiliation></affiliation></author><author><name sortKey="Boulanger, Pierre A" sort="Boulanger, Pierre A" uniqKey="Boulanger P" first="Pierre A." last="Boulanger">Pierre A. Boulanger</name><affiliation><mods:affiliation>Laboratoire de Virologie et Pathogénèse Moléculaires (CNRS URA 1487), Faculté de Médecine, Boulevard Henri IV, 34060 Montpellier, France</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:14549E3ADBFA9B8B8122B30B17D8993C1B55792F</idno><date when="1994" year="1994">1994</date><idno type="doi">10.1006/viro.1994.1400</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-LF7Q1R6G-R/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000826</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000826</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Oligomerization of Recombinant Penton Base of Adenovirus Type 2 and Its Assembly with Fiber in Baculovirus-Infected Cells</title><author><name sortKey="Karayan, Lucie" sort="Karayan, Lucie" uniqKey="Karayan L" first="Lucie" last="Karayan">Lucie Karayan</name><affiliation><mods:affiliation>Laboratoire de Virologie et Pathogénèse Moléculaires (CNRS URA 1487), Faculté de Médecine, Boulevard Henri IV, 34060 Montpellier, France</mods:affiliation></affiliation></author><author><name sortKey="Gay, Bernard" sort="Gay, Bernard" uniqKey="Gay B" first="Bernard" last="Gay">Bernard Gay</name><affiliation><mods:affiliation>Laboratoire de Virologie et Pathogénèse Moléculaires (CNRS URA 1487), Faculté de Médecine, Boulevard Henri IV, 34060 Montpellier, France</mods:affiliation></affiliation></author><author><name sortKey="Gerfaux, Jacqueline" sort="Gerfaux, Jacqueline" uniqKey="Gerfaux J" first="Jacqueline" last="Gerfaux">Jacqueline Gerfaux</name><affiliation><mods:affiliation>Laboratoire de Virologie et Pathogénèse Moléculaires (CNRS URA 1487), Faculté de Médecine, Boulevard Henri IV, 34060 Montpellier, France</mods:affiliation></affiliation></author><author><name sortKey="Boulanger, Pierre A" sort="Boulanger, Pierre A" uniqKey="Boulanger P" first="Pierre A." last="Boulanger">Pierre A. Boulanger</name><affiliation><mods:affiliation>Laboratoire de Virologie et Pathogénèse Moléculaires (CNRS URA 1487), Faculté de Médecine, Boulevard Henri IV, 34060 Montpellier, France</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994">1994</date><biblScope unit="volume">202</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="782">782</biblScope><biblScope unit="page" to="795">795</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Full-length Ad2 penton base (PbFL571) and amino-terminal (PbAT69) and carboxy-terminal deletion mutants (PbCT550 and PbCT531) were expressed at high levels in recombinant baculovirus-infected insect cells. PbFL571, and with a lesser efficiency PbAT69, assembled penton base capsomers (9S pentamers), whereas pentamer assembly was abolished with the deletion of the 21 C-terminal amino acids (as in PbCT550). A discrete population of penton base capsomers in PbFL571 and PbAT69 was found to occur as 12S decamers. PbFL571 and PbAT69 penton base were able to bind to full-length (but not to N-terminal deletion mutants of) fiber trimer to form authentic penton capsomer when coexpressed in trans in double-infected cells. Penton base monomers accumulated by PbCT550 and PbCT531 were capable of interacting with both fiber trimers and monomers. This suggested that mutual binding sites exist in the fiber and penton base subunits, and that the fiber-binding domain is located between residues 70 and 531 in the penton base, with its complementary domain situated at the N-terminus of the fiber. Intranuclear inclusions of recombinant protein were observed with the three deletion mutants PbAT69, PbCT550, and PbCT531, implying the existence of karyophilic signal(s) in the central domain of penton base.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Lucie Karayan</name><affiliations><json:string>Laboratoire de Virologie et Pathogénèse Moléculaires (CNRS URA 1487), Faculté de Médecine, Boulevard Henri IV, 34060 Montpellier, France</json:string></affiliations></json:item><json:item><name>Bernard Gay</name><affiliations><json:string>Laboratoire de Virologie et Pathogénèse Moléculaires (CNRS URA 1487), Faculté de Médecine, Boulevard Henri IV, 34060 Montpellier, France</json:string></affiliations></json:item><json:item><name>Jacqueline Gerfaux</name><affiliations><json:string>Laboratoire de Virologie et Pathogénèse Moléculaires (CNRS URA 1487), Faculté de Médecine, Boulevard Henri IV, 34060 Montpellier, France</json:string></affiliations></json:item><json:item><name>Pierre A. Boulanger</name><affiliations><json:string>Laboratoire de Virologie et Pathogénèse Moléculaires (CNRS URA 1487), Faculté de Médecine, Boulevard Henri IV, 34060 Montpellier, France</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-LF7Q1R6G-R</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: Full-length Ad2 penton base (PbFL571) and amino-terminal (PbAT69) and carboxy-terminal deletion mutants (PbCT550 and PbCT531) were expressed at high levels in recombinant baculovirus-infected insect cells. PbFL571, and with a lesser efficiency PbAT69, assembled penton base capsomers (9S pentamers), whereas pentamer assembly was abolished with the deletion of the 21 C-terminal amino acids (as in PbCT550). A discrete population of penton base capsomers in PbFL571 and PbAT69 was found to occur as 12S decamers. PbFL571 and PbAT69 penton base were able to bind to full-length (but not to N-terminal deletion mutants of) fiber trimer to form authentic penton capsomer when coexpressed in trans in double-infected cells. Penton base monomers accumulated by PbCT550 and PbCT531 were capable of interacting with both fiber trimers and monomers. This suggested that mutual binding sites exist in the fiber and penton base subunits, and that the fiber-binding domain is located between residues 70 and 531 in the penton base, with its complementary domain situated at the N-terminus of the fiber. Intranuclear inclusions of recombinant protein were observed with the three deletion mutants PbAT69, PbCT550, and PbCT531, implying the existence of karyophilic signal(s) in the central domain of penton base.</abstract><qualityIndicators><score>4.378</score><pdfWordCount>0</pdfWordCount><pdfCharCount>0</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>14</pdfPageCount><pdfPageSize>610 x 801 pts</pdfPageSize><refBibsNative>false</refBibsNative><abstractWordCount>194</abstractWordCount><abstractCharCount>1310</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Oligomerization of Recombinant Penton Base of Adenovirus Type 2 and Its Assembly with Fiber in Baculovirus-Infected Cells</title><pmid><json:string>8030241</json:string></pmid><pii><json:string>S0042-6822(84)71400-0</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>Virology</title><language><json:string>unknown</json:string></language><publicationDate>1994</publicationDate><issn><json:string>0042-6822</json:string></issn><pii><json:string>S0042-6822(00)X0162-6</json:string></pii><volume>202</volume><issue>2</issue><pages><first>782</first><last>795</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date></date><geogName></geogName><orgName></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName></persName><placeName></placeName><ref_url></ref_url><ref_bibl></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-LF7Q1R6G-R</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - virology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - virology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Virology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string><json:string>4 - biotechnologie</json:string></inist></categories><publicationDate>1994</publicationDate><copyrightDate>1994</copyrightDate><doi><json:string>10.1006/viro.1994.1400</json:string></doi><id>14549E3ADBFA9B8B8122B30B17D8993C1B55792F</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-LF7Q1R6G-R/fulltext.pdf</uri></json:item><json:item><extension>ocr</extension><original>false</original><mimetype>text/ocr</mimetype><quality><totalToken>9577</totalToken><correct>8044</correct><rate>79.3223266847214</rate><misspelled>1496</misspelled></quality><langDetect><reliable>true</reliable><languages><json:item><score>875</score><code>en</code><name>ENGLISH</name><percent>99</percent></json:item><json:item><score>444</score><code>ro</code><name>ROMANIAN</name><percent>0</percent></json:item></languages></langDetect><uri>https://api.istex.fr/ark:/67375/6H6-LF7Q1R6G-R/fulltext.ocr</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-LF7Q1R6G-R/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-LF7Q1R6G-R/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Oligomerization of Recombinant Penton Base of Adenovirus Type 2 and Its Assembly with Fiber in Baculovirus-Infected Cells</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>©1994 Academic Press</p></licence><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</p></availability><date>1994</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note type="content">Section title: Regular Article</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Oligomerization of Recombinant Penton Base of Adenovirus Type 2 and Its Assembly with Fiber in Baculovirus-Infected Cells</title><author xml:id="author-0000"><persName><forename type="first">Lucie</forename><surname>Karayan</surname></persName><affiliation>Laboratoire de Virologie et Pathogénèse Moléculaires (CNRS URA 1487), Faculté de Médecine, Boulevard Henri IV, 34060 Montpellier, France</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Bernard</forename><surname>Gay</surname></persName><affiliation>Laboratoire de Virologie et Pathogénèse Moléculaires (CNRS URA 1487), Faculté de Médecine, Boulevard Henri IV, 34060 Montpellier, France</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Jacqueline</forename><surname>Gerfaux</surname></persName><affiliation>Laboratoire de Virologie et Pathogénèse Moléculaires (CNRS URA 1487), Faculté de Médecine, Boulevard Henri IV, 34060 Montpellier, France</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Pierre A.</forename><surname>Boulanger</surname></persName><affiliation>Laboratoire de Virologie et Pathogénèse Moléculaires (CNRS URA 1487), Faculté de Médecine, Boulevard Henri IV, 34060 Montpellier, France</affiliation></author><idno type="istex">14549E3ADBFA9B8B8122B30B17D8993C1B55792F</idno><idno type="ark">ark:/67375/6H6-LF7Q1R6G-R</idno><idno type="DOI">10.1006/viro.1994.1400</idno><idno type="PII">S0042-6822(84)71400-0</idno></analytic><monogr><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="pISSN">0042-6822</idno><idno type="PII">S0042-6822(00)X0162-6</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994"></date><biblScope unit="volume">202</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="782">782</biblScope><biblScope unit="page" to="795">795</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1994</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Full-length Ad2 penton base (PbFL571) and amino-terminal (PbAT69) and carboxy-terminal deletion mutants (PbCT550 and PbCT531) were expressed at high levels in recombinant baculovirus-infected insect cells. PbFL571, and with a lesser efficiency PbAT69, assembled penton base capsomers (9S pentamers), whereas pentamer assembly was abolished with the deletion of the 21 C-terminal amino acids (as in PbCT550). A discrete population of penton base capsomers in PbFL571 and PbAT69 was found to occur as 12S decamers. PbFL571 and PbAT69 penton base were able to bind to full-length (but not to N-terminal deletion mutants of) fiber trimer to form authentic penton capsomer when coexpressed in trans in double-infected cells. Penton base monomers accumulated by PbCT550 and PbCT531 were capable of interacting with both fiber trimers and monomers. This suggested that mutual binding sites exist in the fiber and penton base subunits, and that the fiber-binding domain is located between residues 70 and 531 in the penton base, with its complementary domain situated at the N-terminus of the fiber. Intranuclear inclusions of recombinant protein were observed with the three deletion mutants PbAT69, PbCT550, and PbCT531, implying the existence of karyophilic signal(s) in the central domain of penton base.</p></abstract></profileDesc><revisionDesc><change when="1994">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-LF7Q1R6G-R/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier converted-article found"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>YVIRO</jid><aid>71400</aid><ce:pii>S0042-6822(84)71400-0</ce:pii><ce:doi>10.1006/viro.1994.1400</ce:doi><ce:copyright type="full-transfer" year="1994">Academic Press</ce:copyright></item-info><head><ce:dochead><ce:textfn>Regular Article</ce:textfn></ce:dochead><ce:title>Oligomerization of Recombinant Penton Base of Adenovirus Type 2 and Its Assembly with Fiber in Baculovirus-Infected Cells</ce:title><ce:author-group><ce:author><ce:given-name>Lucie</ce:given-name><ce:surname>Karayan</ce:surname></ce:author><ce:author><ce:given-name>Bernard</ce:given-name><ce:surname>Gay</ce:surname></ce:author><ce:author><ce:given-name>Jacqueline</ce:given-name><ce:surname>Gerfaux</ce:surname></ce:author><ce:author><ce:given-name>Pierre A.</ce:given-name><ce:surname>Boulanger</ce:surname></ce:author><ce:affiliation><ce:textfn>Laboratoire de Virologie et Pathogénèse Moléculaires (CNRS URA 1487), Faculté de Médecine, Boulevard Henri IV, 34060 Montpellier, France</ce:textfn></ce:affiliation></ce:author-group><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Full-length Ad2 penton base (PbFL571) and amino-terminal (PbAT69) and carboxy-terminal deletion mutants (PbCT550 and PbCT531) were expressed at high levels in recombinant baculovirus-infected insect cells. PbFL571, and with a lesser efficiency PbAT69, assembled penton base capsomers (9S pentamers), whereas pentamer assembly was abolished with the deletion of the 21 C-terminal amino acids (as in PbCT550). A discrete population of penton base capsomers in PbFL571 and PbAT69 was found to occur as 12S decamers. PbFL571 and PbAT69 penton base were able to bind to full-length (but not to N-terminal deletion mutants of) fiber trimer to form authentic penton capsomer when coexpressed in <ce:italic>trans</ce:italic> in double-infected cells. Penton base monomers accumulated by PbCT550 and PbCT531 were capable of interacting with both fiber trimers and monomers. This suggested that mutual binding sites exist in the fiber and penton base subunits, and that the fiber-binding domain is located between residues 70 and 531 in the penton base, with its complementary domain situated at the N-terminus of the fiber. Intranuclear inclusions of recombinant protein were observed with the three deletion mutants PbAT69, PbCT550, and PbCT531, implying the existence of karyophilic signal(s) in the central domain of penton base.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Oligomerization of Recombinant Penton Base of Adenovirus Type 2 and Its Assembly with Fiber in Baculovirus-Infected Cells</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Oligomerization of Recombinant Penton Base of Adenovirus Type 2 and Its Assembly with Fiber in Baculovirus-Infected Cells</title></titleInfo><name type="personal"><namePart type="given">Lucie</namePart><namePart type="family">Karayan</namePart><affiliation>Laboratoire de Virologie et Pathogénèse Moléculaires (CNRS URA 1487), Faculté de Médecine, Boulevard Henri IV, 34060 Montpellier, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Bernard</namePart><namePart type="family">Gay</namePart><affiliation>Laboratoire de Virologie et Pathogénèse Moléculaires (CNRS URA 1487), Faculté de Médecine, Boulevard Henri IV, 34060 Montpellier, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jacqueline</namePart><namePart type="family">Gerfaux</namePart><affiliation>Laboratoire de Virologie et Pathogénèse Moléculaires (CNRS URA 1487), Faculté de Médecine, Boulevard Henri IV, 34060 Montpellier, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Pierre A.</namePart><namePart type="family">Boulanger</namePart><affiliation>Laboratoire de Virologie et Pathogénèse Moléculaires (CNRS URA 1487), Faculté de Médecine, Boulevard Henri IV, 34060 Montpellier, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1994</dateIssued><copyrightDate encoding="w3cdtf">1994</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Full-length Ad2 penton base (PbFL571) and amino-terminal (PbAT69) and carboxy-terminal deletion mutants (PbCT550 and PbCT531) were expressed at high levels in recombinant baculovirus-infected insect cells. PbFL571, and with a lesser efficiency PbAT69, assembled penton base capsomers (9S pentamers), whereas pentamer assembly was abolished with the deletion of the 21 C-terminal amino acids (as in PbCT550). A discrete population of penton base capsomers in PbFL571 and PbAT69 was found to occur as 12S decamers. PbFL571 and PbAT69 penton base were able to bind to full-length (but not to N-terminal deletion mutants of) fiber trimer to form authentic penton capsomer when coexpressed in trans in double-infected cells. Penton base monomers accumulated by PbCT550 and PbCT531 were capable of interacting with both fiber trimers and monomers. This suggested that mutual binding sites exist in the fiber and penton base subunits, and that the fiber-binding domain is located between residues 70 and 531 in the penton base, with its complementary domain situated at the N-terminus of the fiber. Intranuclear inclusions of recombinant protein were observed with the three deletion mutants PbAT69, PbCT550, and PbCT531, implying the existence of karyophilic signal(s) in the central domain of penton base.</abstract><note type="content">Section title: Regular Article</note><relatedItem type="host"><titleInfo><title>Virology</title></titleInfo><titleInfo type="abbreviated"><title>YVIRO</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1994</dateIssued></originInfo><identifier type="ISSN">0042-6822</identifier><identifier type="PII">S0042-6822(00)X0162-6</identifier><part><date>1994</date><detail type="volume"><number>202</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue-pages"><start>521</start><end>1098</end></extent><extent unit="pages"><start>782</start><end>795</end></extent></part></relatedItem><identifier type="istex">14549E3ADBFA9B8B8122B30B17D8993C1B55792F</identifier><identifier type="ark">ark:/67375/6H6-LF7Q1R6G-R</identifier><identifier type="DOI">10.1006/viro.1994.1400</identifier><identifier type="PII">S0042-6822(84)71400-0</identifier><accessCondition type="use and reproduction" contentType="copyright">©1994 Academic Press</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource><recordOrigin>Academic Press, ©1994</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-LF7Q1R6G-R/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000826 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000826 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:14549E3ADBFA9B8B8122B30B17D8993C1B55792F
|texte= Oligomerization of Recombinant Penton Base of Adenovirus Type 2 and Its Assembly with Fiber in Baculovirus-Infected Cells
}}
| This area was generated with Dilib version V0.6.33. |  |