Sequences of Tobacco Rattle Viruses from Potato
Identifieur interne : 000760 ( Istex/Corpus ); précédent : 000759; suivant : 000761Sequences of Tobacco Rattle Viruses from Potato
Auteurs : C. Heinze ; S. Von Bargen ; M. Sadowska-Rybak ; P. Willingmann ; G. AdamSource :
- Journal of Phytopathology [ 0931-1785 ] ; 2000-09.
Abstract
The RNA2 of the tobacco rattle virus (TRV) isolate ‘Rostock’ (TRV‐R), and the coat protein gene (CP) of TRV isolate ‘Mirow’ (TRV‐M) were sequenced. Both were isolated from potato. Sequence analysis revealed a 5′ noncoding‐region (NCR) and a CP gene, which are among the shortest of any tobravirus RNA2 sequenced so far. Downstream of the CP open reading frame (ORF) there was an ORF for a 8 k protein, that is probably a truncated 9 k protein, followed by an ORF for a 16 k protein and the 3′ NCR. The 16 k protein and the 3′ NCR showed a considerable sequence identity with the 3′ end of TRV RNA1 and RNA2 from other TRV isolates. The high identity in the amino acid sequences of the CPs from TRV‐R and TRV‐M as well as other TRV isolates suggested that they are related to the TCM group of the genus Tobravirus and belong serologically to the RQ‐serotype. This could be confirmed using a rabbit antiserum that was prepared against recombinant TRV‐R CP expressed in bacteria. This serum was found to be suitable for differentiation of TRV‐isolates.
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DOI: 10.1046/j.1439-0434.2000.00559.x
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Sadowska-Rybak</name><affiliation><mods:affiliation>Authors' address: Institut für Angewandte Botanik, Abteilung Pflanzenschutz, Universität Hamburg, Marseillerstr. 7, 20355 Hamburg, Germany</mods:affiliation></affiliation></author><author><name sortKey="Willingmann, P" sort="Willingmann, P" uniqKey="Willingmann P" first="P." last="Willingmann">P. Willingmann</name><affiliation><mods:affiliation>Authors' address: Institut für Angewandte Botanik, Abteilung Pflanzenschutz, Universität Hamburg, Marseillerstr. 7, 20355 Hamburg, Germany</mods:affiliation></affiliation></author><author><name sortKey="Adam, G" sort="Adam, G" uniqKey="Adam G" first="G." last="Adam">G. Adam</name><affiliation><mods:affiliation>Authors' address: Institut für Angewandte Botanik, Abteilung Pflanzenschutz, Universität Hamburg, Marseillerstr. 7, 20355 Hamburg, Germany</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:8D2D7FAE44B975A1524B52997623B09FD8126380</idno><date when="2000" year="2000">2000</date><idno type="doi">10.1046/j.1439-0434.2000.00559.x</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-VSM7MXB3-0/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000760</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000760</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Sequences of Tobacco Rattle Viruses from Potato</title><author><name sortKey="Heinze, C" sort="Heinze, C" uniqKey="Heinze C" first="C." last="Heinze">C. Heinze</name><affiliation><mods:affiliation>Authors' address: Institut für Angewandte Botanik, Abteilung Pflanzenschutz, Universität Hamburg, Marseillerstr. 7, 20355 Hamburg, Germany</mods:affiliation></affiliation></author><author><name sortKey="Von Bargen, S" sort="Von Bargen, S" uniqKey="Von Bargen S" first="S." last="Von Bargen">S. Von Bargen</name><affiliation><mods:affiliation>Authors' address: Institut für Angewandte Botanik, Abteilung Pflanzenschutz, Universität Hamburg, Marseillerstr. 7, 20355 Hamburg, Germany</mods:affiliation></affiliation></author><author><name sortKey="Sadowska Ybak, M" sort="Sadowska Ybak, M" uniqKey="Sadowska Ybak M" first="M." last="Sadowska-Rybak">M. 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Adam</name><affiliation><mods:affiliation>Authors' address: Institut für Angewandte Botanik, Abteilung Pflanzenschutz, Universität Hamburg, Marseillerstr. 7, 20355 Hamburg, Germany</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Phytopathology</title><title level="j" type="alt">JOURNAL OF PHYTOPATHOLOGY</title><idno type="ISSN">0931-1785</idno><idno type="eISSN">1439-0434</idno><imprint><biblScope unit="vol">148</biblScope><biblScope unit="issue">9‐10</biblScope><biblScope unit="page" from="547">547</biblScope><biblScope unit="page" to="554">554</biblScope><publisher>Blackwell Science Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2000-09">2000-09</date></imprint><idno type="ISSN">0931-1785</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0931-1785</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The RNA2 of the tobacco rattle virus (TRV) isolate ‘Rostock’ (TRV‐R), and the coat protein gene (CP) of TRV isolate ‘Mirow’ (TRV‐M) were sequenced. Both were isolated from potato. Sequence analysis revealed a 5′ noncoding‐region (NCR) and a CP gene, which are among the shortest of any tobravirus RNA2 sequenced so far. Downstream of the CP open reading frame (ORF) there was an ORF for a 8 k protein, that is probably a truncated 9 k protein, followed by an ORF for a 16 k protein and the 3′ NCR. The 16 k protein and the 3′ NCR showed a considerable sequence identity with the 3′ end of TRV RNA1 and RNA2 from other TRV isolates. The high identity in the amino acid sequences of the CPs from TRV‐R and TRV‐M as well as other TRV isolates suggested that they are related to the TCM group of the genus Tobravirus and belong serologically to the RQ‐serotype. This could be confirmed using a rabbit antiserum that was prepared against recombinant TRV‐R CP expressed in bacteria. 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<ref target="http://www.tei-c.org/">We use TEI</ref></desc></application></appInfo></encodingDesc><profileDesc><abstract xml:lang="en" style="main"><p>The RNA2 of the tobacco rattle virus (TRV) isolate ‘Rostock’ (TRV‐R), and the coat protein gene (CP) of TRV isolate ‘Mirow’ (TRV‐M) were sequenced. Both were isolated from potato. Sequence analysis revealed a 5′ noncoding‐region (NCR) and a CP gene, which are among the shortest of any tobravirus RNA2 sequenced so far. Downstream of the CP open reading frame (ORF) there was an ORF for a 8 k protein, that is probably a truncated 9 k protein, followed by an ORF for a 16 k protein and the 3′ NCR. The 16 k protein and the 3′ NCR showed a considerable sequence identity with the 3′ end of TRV RNA1 and RNA2 from other TRV isolates. The high identity in the amino acid sequences of the CPs from TRV‐R and TRV‐M as well as other TRV isolates suggested that they are related to the TCM group of the genus <hi rend="italic">Tobravirus</hi> and belong serologically to the RQ‐serotype. This could be confirmed using a rabbit antiserum that was prepared against recombinant TRV‐R CP expressed in bacteria. This serum was found to be suitable for differentiation of TRV‐isolates.</p></abstract><textClass><keywords rend="tocHeading1"><term>Original Articles</term></keywords></textClass><langUsage><language ident="en"></language></langUsage></profileDesc><revisionDesc><change when="2019-12-20" who="#istex" xml:id="pub2tei">formatting</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-VSM7MXB3-0/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley component found"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Science Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1439-0434</doi><issn type="print">0931-1785</issn><issn type="electronic">1439-0434</issn><idGroup><id type="product" value="JPH"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="JOURNAL OF PHYTOPATHOLOGY">Journal of Phytopathology</title></titleGroup></publicationMeta><publicationMeta level="part" position="09009"><doi origin="wiley">10.1111/jph.2000.148.issue-9-10</doi><numberingGroup><numbering type="journalVolume" number="148">148</numbering><numbering type="journalIssue">9‐10</numbering></numberingGroup><coverDate startDate="2000-09">September 2000</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="0054700" status="forIssue"><doi origin="wiley">10.1046/j.1439-0434.2000.00559.x</doi><idGroup><id type="unit" value="JPH559"></id></idGroup><titleGroup><title type="tocHeading1">Original Articles</title></titleGroup><eventGroup><event type="firstOnline" date="2001-12-25"></event><event type="publishedOnlineFinalForm" date="2001-12-25"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.2 mode:FullText source:Header result:Header" date="2010-03-10"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:4.0.1" date="2014-03-20"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-30"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="547">547</numbering><numbering type="pageLast" number="554">554</numbering></numberingGroup><linkGroup><link type="toTypesetVersion" href="file:JPH.JPH559.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="0"></count><count type="tableTotal" number="0"></count><count type="referenceTotal" number="0"></count><count type="linksPubMed" number="0"></count><count type="linksCrossRef" number="0"></count></countGroup><titleGroup><title type="main">Sequences of Tobacco Rattle Viruses from Potato</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#aff-1-1"><personName><givenNames>C.</givenNames><familyName>Heinze</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#aff-1-1"><personName><givenNames>S.</givenNames><familyName>Von Bargen</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#aff-1-1"><personName><givenNames>M.</givenNames><familyName>Sadowska‐Rybak</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#aff-1-1"><personName><givenNames>P.</givenNames><familyName>Willingmann</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#aff-1-1"><personName><givenNames>G.</givenNames><familyName>Adam</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="aff-1-1" countryCode="DE"><unparsedAffiliation>Authors' address: Institut für Angewandte Botanik, Abteilung Pflanzenschutz, Universität Hamburg, Marseillerstr. 7, 20355 Hamburg, Germany</unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><p>The RNA2 of the tobacco rattle virus (TRV) isolate ‘Rostock’ (TRV‐R), and the coat protein gene (CP) of TRV isolate ‘Mirow’ (TRV‐M) were sequenced. Both were isolated from potato. Sequence analysis revealed a 5′ noncoding‐region (NCR) and a CP gene, which are among the shortest of any tobravirus RNA2 sequenced so far. Downstream of the CP open reading frame (ORF) there was an ORF for a 8 k protein, that is probably a truncated 9 k protein, followed by an ORF for a 16 k protein and the 3′ NCR. The 16 k protein and the 3′ NCR showed a considerable sequence identity with the 3′ end of TRV RNA1 and RNA2 from other TRV isolates. The high identity in the amino acid sequences of the CPs from TRV‐R and TRV‐M as well as other TRV isolates suggested that they are related to the TCM group of the genus <i>Tobravirus</i> and belong serologically to the RQ‐serotype. This could be confirmed using a rabbit antiserum that was prepared against recombinant TRV‐R CP expressed in bacteria. This serum was found to be suitable for differentiation of TRV‐isolates.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Sequences of Tobacco Rattle Viruses from Potato</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Sequences of Tobacco Rattle Viruses from Potato</title></titleInfo><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Heinze</namePart><affiliation>Authors' address: Institut für Angewandte Botanik, Abteilung Pflanzenschutz, Universität Hamburg, Marseillerstr. 7, 20355 Hamburg, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Von Bargen</namePart><affiliation>Authors' address: Institut für Angewandte Botanik, Abteilung Pflanzenschutz, Universität Hamburg, Marseillerstr. 7, 20355 Hamburg, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Sadowska‐Rybak</namePart><affiliation>Authors' address: Institut für Angewandte Botanik, Abteilung Pflanzenschutz, Universität Hamburg, Marseillerstr. 7, 20355 Hamburg, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Willingmann</namePart><affiliation>Authors' address: Institut für Angewandte Botanik, Abteilung Pflanzenschutz, Universität Hamburg, Marseillerstr. 7, 20355 Hamburg, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="family">Adam</namePart><affiliation>Authors' address: Institut für Angewandte Botanik, Abteilung Pflanzenschutz, Universität Hamburg, Marseillerstr. 7, 20355 Hamburg, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>Blackwell Science Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2000-09</dateIssued><copyrightDate encoding="w3cdtf">2000</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><extent unit="figures">0</extent><extent unit="tables">0</extent><extent unit="references">0</extent><extent unit="linksCrossRef">0</extent></physicalDescription><abstract lang="en">The RNA2 of the tobacco rattle virus (TRV) isolate ‘Rostock’ (TRV‐R), and the coat protein gene (CP) of TRV isolate ‘Mirow’ (TRV‐M) were sequenced. Both were isolated from potato. Sequence analysis revealed a 5′ noncoding‐region (NCR) and a CP gene, which are among the shortest of any tobravirus RNA2 sequenced so far. Downstream of the CP open reading frame (ORF) there was an ORF for a 8 k protein, that is probably a truncated 9 k protein, followed by an ORF for a 16 k protein and the 3′ NCR. The 16 k protein and the 3′ NCR showed a considerable sequence identity with the 3′ end of TRV RNA1 and RNA2 from other TRV isolates. The high identity in the amino acid sequences of the CPs from TRV‐R and TRV‐M as well as other TRV isolates suggested that they are related to the TCM group of the genus Tobravirus and belong serologically to the RQ‐serotype. This could be confirmed using a rabbit antiserum that was prepared against recombinant TRV‐R CP expressed in bacteria. This serum was found to be suitable for differentiation of TRV‐isolates.</abstract><relatedItem type="host"><titleInfo><title>Journal of Phytopathology</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><identifier type="ISSN">0931-1785</identifier><identifier type="eISSN">1439-0434</identifier><identifier type="DOI">10.1111/(ISSN)1439-0434</identifier><identifier type="PublisherID">JPH</identifier><part><date>2000</date><detail type="volume"><caption>vol.</caption><number>148</number></detail><detail type="issue"><caption>no.</caption><number>9‐10</number></detail><extent unit="pages"><start>547</start><end>554</end></extent></part></relatedItem><identifier type="istex">8D2D7FAE44B975A1524B52997623B09FD8126380</identifier><identifier type="ark">ark:/67375/WNG-VSM7MXB3-0</identifier><identifier type="DOI">10.1046/j.1439-0434.2000.00559.x</identifier><identifier type="ArticleID">JPH559</identifier><accessCondition type="use and reproduction" contentType="copyright">© Wiley. 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