Gene interference using antisense oligodeoxynucleotides on whole chick embryos
Identifieur interne : 000694 ( Istex/Corpus ); précédent : 000693; suivant : 000695Gene interference using antisense oligodeoxynucleotides on whole chick embryos
Auteurs : Jonathan Cooke ; Alison IsaacSource :
- Molecular Biotechnology [ 1073-6085 ] ; 2000-07-01.
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Abstract
Abstract: Details are given of an advanced version of the ring method of chick embryo culture. This ensures good development from early blastoderm stages even when the culturing procedure is interrupted by the extended periods required for collecting matched embryo samples and for preparing antisense treatment. Antisense oligodeoxynucleotide treatment, in short-term incubation before return of blastoderms to their ring cultures, is then described. An alternative, roller-bottle, culture method for continued development after treatment is also described. Criteria for the validity and success of this gene interference method are given. While the toxt is meant to be of detailed prootioal help to these inexperienced in ombryo culture a preliminary reading, and familiarity with its sectional (Subheading) structure, is recommended before work is undertaken.
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DOI: 10.1385/MB:15:3:259
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This ensures good development from early blastoderm stages even when the culturing procedure is interrupted by the extended periods required for collecting matched embryo samples and for preparing antisense treatment. Antisense oligodeoxynucleotide treatment, in short-term incubation before return of blastoderms to their ring cultures, is then described. An alternative, roller-bottle, culture method for continued development after treatment is also described. Criteria for the validity and success of this gene interference method are given. While the toxt is meant to be of detailed prootioal help to these inexperienced in ombryo culture a preliminary reading, and familiarity with its sectional (Subheading) structure, is recommended before work is undertaken.</Para></Abstract><KeywordGroup Language="En"><Heading>Index Entries</Heading><Keyword>Antisense ogligodeoxynucleotides (ODNs)</Keyword><Keyword>gene interference</Keyword><Keyword>chick embryos</Keyword><Keyword>embryo culture techniques</Keyword></KeywordGroup></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Gene interference using antisense oligodeoxynucleotides on whole chick embryos</title><subTitle>Optimal ring and roller-bottle culture technique</subTitle></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Gene interference using antisense oligodeoxynucleotides on whole chick embryos</title></titleInfo><name type="personal" displayLabel="corresp"><namePart type="given">Jonathan</namePart><namePart type="family">Cooke</namePart><affiliation>National Institute for medical Research, The Ridgeway, Mill Hill, NW7 1AA, London, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Alison</namePart><namePart type="family">Isaac</namePart><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Humana Press</publisher><place><placeTerm type="text">Totowa</placeTerm></place><dateIssued encoding="w3cdtf">2000-07-01</dateIssued><copyrightDate encoding="w3cdtf">2000</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: Details are given of an advanced version of the ring method of chick embryo culture. This ensures good development from early blastoderm stages even when the culturing procedure is interrupted by the extended periods required for collecting matched embryo samples and for preparing antisense treatment. Antisense oligodeoxynucleotide treatment, in short-term incubation before return of blastoderms to their ring cultures, is then described. An alternative, roller-bottle, culture method for continued development after treatment is also described. Criteria for the validity and success of this gene interference method are given. 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