Single‐step nested polymerase chain reaction for detection of different genotypes of hepatitis C virus
Identifieur interne : 000583 ( Istex/Corpus ); précédent : 000582; suivant : 000584Single‐step nested polymerase chain reaction for detection of different genotypes of hepatitis C virus
Auteurs : Ji-Su Li ; Shu-Ping Tong ; Ludmila Vitvitski ; Christian TrépoSource :
- Journal of Medical Virology [ 0146-6615 ] ; 1995-02.
Abstract
Hepatitis C virus (HCV) exhibits considerable sequence variability and circulates in the blood at extremely low levels. Current methods for detecting HCV RNA are based mostly on nested polymerase chain reaction (PCR), in which part of the first amplification product is reamplified in the second tube by an internal primer pair. A novel nested PCR method was developed in which the two successive amplification processes are carried out in the same tube with a single step of physical manipulation. Careful selection of highly conserved sequences of the 5′ noncoding region as primers enabled successful detection of all three major genotypes circulating in France, including the one with variation in this region. Retaining high sensitivity of the conventional nested PCR, the novel method reduced greatly the risk of carry‐over contaminations. It was also cost‐ and time‐saving. The one‐step nested PCR method is especially suitable for routine diagnosis of HCV infection in clinical laboratories. © 1995 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jmv.1890450207
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Current methods for detecting HCV RNA are based mostly on nested polymerase chain reaction (PCR), in which part of the first amplification product is reamplified in the second tube by an internal primer pair. A novel nested PCR method was developed in which the two successive amplification processes are carried out in the same tube with a single step of physical manipulation. Careful selection of highly conserved sequences of the 5′ noncoding region as primers enabled successful detection of all three major genotypes circulating in France, including the one with variation in this region. Retaining high sensitivity of the conventional nested PCR, the novel method reduced greatly the risk of carry‐over contaminations. It was also cost‐ and time‐saving. 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Thomas, 69424 Lyon cedex 03, France===</json:string></affiliations></json:item><json:item><name>Shu‐Ping Tong</name><affiliations><json:string>Unité de Recherche sur les Hépatites INSERM U271, Lyon, France</json:string><json:string>Current Address: Molecular Hepatology Laboratory, Massachusetts General Hospital Cancer Center, Harvard Medical School, Charlestown, MA 02129</json:string></affiliations></json:item><json:item><name>Ludmila Vitvitski</name><affiliations><json:string>Unité de Recherche sur les Hépatites INSERM U271, Lyon, France</json:string></affiliations></json:item><json:item><name>Christian Trépo</name><affiliations><json:string>Unité de Recherche sur les Hépatites INSERM U271, Lyon, France</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>non‐A</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>non‐B hepatitis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>enzymatic amplification</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>clinical diagnosis, contamination, methodology</value></json:item></subject><articleId><json:string>JMV1890450207</json:string></articleId><arkIstex>ark:/67375/WNG-67GG3H3B-J</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Hepatitis C virus (HCV) exhibits considerable sequence variability and circulates in the blood at extremely low levels. Current methods for detecting HCV RNA are based mostly on nested polymerase chain reaction (PCR), in which part of the first amplification product is reamplified in the second tube by an internal primer pair. A novel nested PCR method was developed in which the two successive amplification processes are carried out in the same tube with a single step of physical manipulation. Careful selection of highly conserved sequences of the 5′ noncoding region as primers enabled successful detection of all three major genotypes circulating in France, including the one with variation in this region. Retaining high sensitivity of the conventional nested PCR, the novel method reduced greatly the risk of carry‐over contaminations. It was also cost‐ and time‐saving. 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[1992]</json:string><json:string>Dusheiko et al., 19941</json:string><json:string>Okamoto et al., 1992</json:string><json:string>Li et al., 19911</json:string><json:string>Weiner et al., 19901</json:string><json:string>Cristiano et al., 1991</json:string><json:string>Li et al., 1991, 19941</json:string><json:string>Li et al.</json:string><json:string>Hu et al., 19943</json:string><json:string>Chan et al., 1992</json:string><json:string>Li et al., 19921</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/WNG-67GG3H3B-J</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - virology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - virology</json:string></scienceMetrix><scopus><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - Infectious 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Virol.</title></titleGroup></publicationMeta><publicationMeta level="part" position="20"><doi origin="wiley" registered="yes">10.1002/jmv.v45:2</doi><numberingGroup><numbering type="journalVolume" number="45">45</numbering><numbering type="journalIssue">2</numbering></numberingGroup><coverDate startDate="1995-02">February 1995</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="7" status="forIssue"><doi origin="wiley" registered="yes">10.1002/jmv.1890450207</doi><idGroup><id type="unit" value="JMV1890450207"></id></idGroup><countGroup><count type="pageTotal" number="5"></count></countGroup><titleGroup><title type="articleCategory">Article</title><title type="tocHeading1">Articles</title></titleGroup><copyright ownership="publisher">Copyright © 1995 Wiley‐Liss, Inc., A Wiley Company</copyright><eventGroup><event type="manuscriptAccepted" date="1994-07-21"></event><event type="firstOnline" date="2005-12-07"></event><event type="publishedOnlineFinalForm" date="2005-12-07"></event><event type="xmlConverted" agent="Converter:JWSART34_TO_WML3G version:2.3.2 mode:FullText source:HeaderRef result:HeaderRef" date="2010-03-15"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:4.0.1" date="2014-03-20"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-30"></event></eventGroup><numberingGroup><numbering type="pageFirst">151</numbering><numbering type="pageLast">155</numbering></numberingGroup><correspondenceTo>INSERM U271, 151 Cours A. Thomas, 69424 Lyon cedex 03, France===</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JMV.JMV1890450207.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="2"></count><count type="tableTotal" number="1"></count><count type="referenceTotal" number="22"></count></countGroup><titleGroup><title type="main" xml:lang="en">Single‐step nested polymerase chain reaction for detection of different genotypes of hepatitis C virus</title><title type="short" xml:lang="en">Modified Nested PCR for HCV RNA</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1" currentRef="#curr1" corresponding="yes"><personName><givenNames>Ji‐Su</givenNames><familyName>Li</familyName></personName></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#af1" currentRef="#curr1"><personName><givenNames>Shu‐Ping</givenNames><familyName>Tong</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Ludmila</givenNames><familyName>Vitvitski</familyName></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Christian</givenNames><familyName>Trépo</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="af1" countryCode="FR" type="organization"><unparsedAffiliation>Unité de Recherche sur les Hépatites INSERM U271, Lyon, France</unparsedAffiliation></affiliation><affiliation xml:id="curr1" countryCode="US"><unparsedAffiliation>Molecular Hepatology Laboratory, Massachusetts General Hospital Cancer Center, Harvard Medical School, Charlestown, MA 02129</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">non‐A</keyword><keyword xml:id="kwd2">non‐B hepatitis</keyword><keyword xml:id="kwd3">enzymatic amplification</keyword><keyword xml:id="kwd4">clinical diagnosis, contamination, methodology</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Hepatitis C virus (HCV) exhibits considerable sequence variability and circulates in the blood at extremely low levels. Current methods for detecting HCV RNA are based mostly on nested polymerase chain reaction (PCR), in which part of the first amplification product is reamplified in the second tube by an internal primer pair. A novel nested PCR method was developed in which the two successive amplification processes are carried out in the same tube with a single step of physical manipulation. Careful selection of highly conserved sequences of the 5′ noncoding region as primers enabled successful detection of all three major genotypes circulating in France, including the one with variation in this region. Retaining high sensitivity of the conventional nested PCR, the novel method reduced greatly the risk of carry‐over contaminations. It was also cost‐ and time‐saving. The one‐step nested PCR method is especially suitable for routine diagnosis of HCV infection in clinical laboratories. © 1995 Wiley‐Liss, Inc.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Single‐step nested polymerase chain reaction for detection of different genotypes of hepatitis C virus</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Modified Nested PCR for HCV RNA</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Single‐step nested polymerase chain reaction for detection of different genotypes of hepatitis C virus</title></titleInfo><name type="personal"><namePart type="given">Ji‐Su</namePart><namePart type="family">Li</namePart><affiliation>Unité de Recherche sur les Hépatites INSERM U271, Lyon, France</affiliation><affiliation>Current Address: Molecular Hepatology Laboratory, Massachusetts General Hospital Cancer Center, Harvard Medical School, Charlestown, MA 02129</affiliation><affiliation>Correspondence address: INSERM U271, 151 Cours A. Thomas, 69424 Lyon cedex 03, France===</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Shu‐Ping</namePart><namePart type="family">Tong</namePart><affiliation>Unité de Recherche sur les Hépatites INSERM U271, Lyon, France</affiliation><affiliation>Current Address: Molecular Hepatology Laboratory, Massachusetts General Hospital Cancer Center, Harvard Medical School, Charlestown, MA 02129</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ludmila</namePart><namePart type="family">Vitvitski</namePart><affiliation>Unité de Recherche sur les Hépatites INSERM U271, Lyon, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Christian</namePart><namePart type="family">Trépo</namePart><affiliation>Unité de Recherche sur les Hépatites INSERM U271, Lyon, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><place><placeTerm type="text">New York</placeTerm></place><dateIssued encoding="w3cdtf">1995-02</dateIssued><dateValid encoding="w3cdtf">1994-07-21</dateValid><copyrightDate encoding="w3cdtf">1995</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><extent unit="figures">2</extent><extent unit="tables">1</extent><extent unit="references">22</extent></physicalDescription><abstract lang="en">Hepatitis C virus (HCV) exhibits considerable sequence variability and circulates in the blood at extremely low levels. Current methods for detecting HCV RNA are based mostly on nested polymerase chain reaction (PCR), in which part of the first amplification product is reamplified in the second tube by an internal primer pair. A novel nested PCR method was developed in which the two successive amplification processes are carried out in the same tube with a single step of physical manipulation. Careful selection of highly conserved sequences of the 5′ noncoding region as primers enabled successful detection of all three major genotypes circulating in France, including the one with variation in this region. Retaining high sensitivity of the conventional nested PCR, the novel method reduced greatly the risk of carry‐over contaminations. It was also cost‐ and time‐saving. The one‐step nested PCR method is especially suitable for routine diagnosis of HCV infection in clinical laboratories. © 1995 Wiley‐Liss, Inc.</abstract><subject lang="en"><genre>keywords</genre><topic>non‐A</topic><topic>non‐B hepatitis</topic><topic>enzymatic amplification</topic><topic>clinical diagnosis, contamination, methodology</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Medical Virology</title></titleInfo><titleInfo type="abbreviated"><title>J. Med. 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Journal of Clinical Microbiology 31: 882–886.</note><part><date>1993</date><detail type="volume"><caption>vol.</caption><number>31</number></detail><extent unit="pages"><start>882</start><end>886</end></extent></part><relatedItem type="host"><titleInfo><title>Journal of Clinical Microbiology</title></titleInfo><part><date>1993</date><detail type="volume"><caption>vol.</caption><number>31</number></detail><extent unit="pages"><start>882</start><end>886</end></extent></part></relatedItem></relatedItem><identifier type="istex">92260B2629F9E5C793E467E5C939F167795C934B</identifier><identifier type="ark">ark:/67375/WNG-67GG3H3B-J</identifier><identifier type="DOI">10.1002/jmv.1890450207</identifier><identifier type="ArticleID">JMV1890450207</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 1995 Wiley‐Liss, Inc., A Wiley Company</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-L0C46X92-X">wiley</recordContentSource><recordOrigin>Converted from (version ) to MODS version 3.6.</recordOrigin><recordCreationDate encoding="w3cdtf">2019-11-15</recordCreationDate></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-67GG3H3B-J/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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|texte= Single‐step nested polymerase chain reaction for detection of different genotypes of hepatitis C virus
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