Antisense oligonucleotides: gene regulation and chemotherapy of AIDS
Identifieur interne : 000398 ( Istex/Corpus ); précédent : 000397; suivant : 000399Antisense oligonucleotides: gene regulation and chemotherapy of AIDS
Auteurs : Sudhir Agrawal ; Prem S. SarinSource :
- Advanced Drug Delivery Reviews [ 0169-409X ] ; 1991.
English descriptors
- Teeft :
- Acad, Acceptor, Agrawal, Analogue, Antisense, Antisense oligomers, Antisense oligonucleotides, Antisense sequence, Assay, Biochemistry, Body weight, Cell culture, Chem, Chemotherapy, Cholesterol residue, Cytopathic, Cytopathic effect, Duplex, Gene regulation, Gene regulationand chemotherapyof aids, Higher concentration, Human immunodeficiency virus, Ids0, Inhibition, Internucleotide linkage, Lett, Linkage, Methylphospho, Methylphosphonate, Methylphosphonate linkage, Methylphosphonate linkages, Mrna, Natl, Nuclease, Nucleic, Nucleic acids, Oligodeoxynucleotide, Oligomer, Oligomers, Oligonucleotide, Oligonucleotide methylphospho triester, Oligonucleotide phosphorothioates, Oligonucleotides, Phosphoroselenoate, Phosphorothioate, Phosphorothioate analogue, Phosphorothioate oligomer, Phosphorothioate oligomers, Proc, Protein synthesis, Replication, Sarin, Similar results, Splice, Splice acceptor site, Splice donor site, Syncytia formation, Syncytium, Tissue culture, Toxicity, Transcriptase, Transcriptase assay, Transcription, Triester, Trna, Unmodified, Unmodified oligomer, Unmodified oligonucleotide, Unmodified oligonucleotides, Viral, Virus replication, Zamecnik.
Abstract
Abstract: The use of antisense oligonucleotides in control of gene regulation and inhibition of virus replication is a unique approach designed to interfere with cell and virus replication, transcription and translation machinery at the molecular level. Extensive studies ave been carried out to regulate replication of human immunodeficiency virus by synthetic oligonucleotides and their several sugar phosphate backbone modified analogues. Thus far, studies have been carried out in tissue culture, and inhibition of HIV expression has been monitored by several parameters. Oligonucleotide phosphorothioates are effective in inhibiting HIV expression in tissue culture at a concentration of 1·10−7 M, which translates into a dose of approximately 0.6 mg/kg bodyweight. A non-toxic dose of 100 mg/kg, in preliminary acute toxicity experiments, represents an exploitable therapeutic window for ‘oligonucleotide therapy’.
Url:
DOI: 10.1016/0169-409X(91)90020-D
Links to Exploration step
ISTEX:50A1BA6B6CFCE21A80880ECCEB083AC5D2C8CFC8Le document en format XML
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Sarin</name><affiliation><mods:affiliation>Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, MD, U.S.A.</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Advanced Drug Delivery Reviews</title><title level="j" type="abbrev">ADR</title><idno type="ISSN">0169-409X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1991">1991</date><biblScope unit="volume">6</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="251">251</biblScope><biblScope unit="page" to="270">270</biblScope></imprint><idno type="ISSN">0169-409X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0169-409X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acad</term><term>Acceptor</term><term>Agrawal</term><term>Analogue</term><term>Antisense</term><term>Antisense oligomers</term><term>Antisense oligonucleotides</term><term>Antisense sequence</term><term>Assay</term><term>Biochemistry</term><term>Body weight</term><term>Cell culture</term><term>Chem</term><term>Chemotherapy</term><term>Cholesterol residue</term><term>Cytopathic</term><term>Cytopathic effect</term><term>Duplex</term><term>Gene regulation</term><term>Gene regulationand chemotherapyof aids</term><term>Higher concentration</term><term>Human immunodeficiency virus</term><term>Ids0</term><term>Inhibition</term><term>Internucleotide linkage</term><term>Lett</term><term>Linkage</term><term>Methylphospho</term><term>Methylphosphonate</term><term>Methylphosphonate linkage</term><term>Methylphosphonate linkages</term><term>Mrna</term><term>Natl</term><term>Nuclease</term><term>Nucleic</term><term>Nucleic acids</term><term>Oligodeoxynucleotide</term><term>Oligomer</term><term>Oligomers</term><term>Oligonucleotide</term><term>Oligonucleotide methylphospho triester</term><term>Oligonucleotide phosphorothioates</term><term>Oligonucleotides</term><term>Phosphoroselenoate</term><term>Phosphorothioate</term><term>Phosphorothioate analogue</term><term>Phosphorothioate oligomer</term><term>Phosphorothioate oligomers</term><term>Proc</term><term>Protein synthesis</term><term>Replication</term><term>Sarin</term><term>Similar results</term><term>Splice</term><term>Splice acceptor site</term><term>Splice donor site</term><term>Syncytia formation</term><term>Syncytium</term><term>Tissue culture</term><term>Toxicity</term><term>Transcriptase</term><term>Transcriptase assay</term><term>Transcription</term><term>Triester</term><term>Trna</term><term>Unmodified</term><term>Unmodified oligomer</term><term>Unmodified oligonucleotide</term><term>Unmodified oligonucleotides</term><term>Viral</term><term>Virus replication</term><term>Zamecnik</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The use of antisense oligonucleotides in control of gene regulation and inhibition of virus replication is a unique approach designed to interfere with cell and virus replication, transcription and translation machinery at the molecular level. Extensive studies ave been carried out to regulate replication of human immunodeficiency virus by synthetic oligonucleotides and their several sugar phosphate backbone modified analogues. Thus far, studies have been carried out in tissue culture, and inhibition of HIV expression has been monitored by several parameters. Oligonucleotide phosphorothioates are effective in inhibiting HIV expression in tissue culture at a concentration of 1·10−7 M, which translates into a dose of approximately 0.6 mg/kg bodyweight. 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Extensive studies ave been carried out to regulate replication of human immunodeficiency virus by synthetic oligonucleotides and their several sugar phosphate backbone modified analogues. Thus far, studies have been carried out in tissue culture, and inhibition of HIV expression has been monitored by several parameters. Oligonucleotide phosphorothioates are effective in inhibiting HIV expression in tissue culture at a concentration of 1·10−7 M, which translates into a dose of approximately 0.6 mg/kg bodyweight. 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Agrawal, Worcester Foundation for Experimental Biology, 222 Maple Avenue, Shrewsbury, MA 01545, U.S.A.</p></note><affiliation>Worcester Foundation for Experimental Biology, Shrewsbury, MA, U.S.A.</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Prem S.</forename><surname>Sarin</surname></persName><affiliation>Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, MD, U.S.A.</affiliation></author><idno type="istex">50A1BA6B6CFCE21A80880ECCEB083AC5D2C8CFC8</idno><idno type="ark">ark:/67375/6H6-DR5LG84V-0</idno><idno type="DOI">10.1016/0169-409X(91)90020-D</idno><idno type="PII">0169-409X(91)90020-D</idno></analytic><monogr><title level="j">Advanced Drug Delivery Reviews</title><title level="j" type="abbrev">ADR</title><idno type="pISSN">0169-409X</idno><idno type="PII">S0169-409X(00)X0101-9</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1991"></date><biblScope unit="volume">6</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="251">251</biblScope><biblScope unit="page" to="270">270</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1991</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: The use of antisense oligonucleotides in control of gene regulation and inhibition of virus replication is a unique approach designed to interfere with cell and virus replication, transcription and translation machinery at the molecular level. Extensive studies ave been carried out to regulate replication of human immunodeficiency virus by synthetic oligonucleotides and their several sugar phosphate backbone modified analogues. Thus far, studies have been carried out in tissue culture, and inhibition of HIV expression has been monitored by several parameters. Oligonucleotide phosphorothioates are effective in inhibiting HIV expression in tissue culture at a concentration of 1·10−7 M, which translates into a dose of approximately 0.6 mg/kg bodyweight. A non-toxic dose of 100 mg/kg, in preliminary acute toxicity experiments, represents an exploitable therapeutic window for ‘oligonucleotide therapy’.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Antisense oligonucleotide</term></item><item><term>Human immunodeficiency virus</term></item><item><term>Chemotherapy of AIDS</term></item><item><term>Phosphorothioate</term></item><item><term>Methylphosphonate</term></item><item><term>Phosphoramidate</term></item><item><term>Inhibition of human immunodeficiency virus</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list><head>Abbreviations</head><item><term>AUG, initiation codon</term></item><item><term>AZT, zidovudine</term></item><item><term>DMF, dimethylformamide</term></item><item><term>Fmoc, 9-fluorenylmethoxycarbonyl</term></item><item><term>HIV, human immunodeficiency virus</term></item><item><term>nef or NEF, negative regulation factor</term></item><item><term>PBS, primer binding site</term></item><item><term>RIPA, radioimmunoprecipitation assay</term></item><item><term>TAR, transactivator responsive region</term></item><item><term>tRNA, transfer RNA</term></item><item><term>vif or VIF, viral infectivity factor</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1991">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-DR5LG84V-0/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="rev"><item-info><jid>ADR</jid><aid>9190020D</aid><ce:pii>0169-409X(91)90020-D</ce:pii><ce:doi>10.1016/0169-409X(91)90020-D</ce:doi><ce:copyright type="unknown" year="1991"></ce:copyright></item-info><head><ce:title>Antisense oligonucleotides: gene regulation and chemotherapy of AIDS</ce:title><ce:author-group><ce:author><ce:given-name>Sudhir</ce:given-name><ce:surname>Agrawal</ce:surname><ce:cross-ref refid="COR1"><ce:sup>∗</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Prem S.</ce:given-name><ce:surname>Sarin</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Worcester Foundation for Experimental Biology, Shrewsbury, MA, U.S.A.</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, MD, U.S.A.</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>∗</ce:label><ce:text>Correspondence: S. Agrawal, Worcester Foundation for Experimental Biology, 222 Maple Avenue, Shrewsbury, MA 01545, U.S.A.</ce:text></ce:correspondence></ce:author-group><ce:date-received day="24" month="7" year="1990"></ce:date-received><ce:date-accepted day="1" month="10" year="1990"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>The use of antisense oligonucleotides in control of gene regulation and inhibition of virus replication is a unique approach designed to interfere with cell and virus replication, transcription and translation machinery at the molecular level. Extensive studies ave been carried out to regulate replication of human immunodeficiency virus by synthetic oligonucleotides and their several sugar phosphate backbone modified analogues. Thus far, studies have been carried out in tissue culture, and inhibition of HIV expression has been monitored by several parameters. Oligonucleotide phosphorothioates are effective in inhibiting HIV expression in tissue culture at a concentration of 1·10<ce:sup>−7</ce:sup> M, which translates into a dose of approximately 0.6 mg/kg bodyweight. A non-toxic dose of 100 mg/kg, in preliminary acute toxicity experiments, represents an exploitable therapeutic window for ‘oligonucleotide therapy’.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Antisense oligonucleotide</ce:text></ce:keyword><ce:keyword><ce:text>Human immunodeficiency virus</ce:text></ce:keyword><ce:keyword><ce:text>Chemotherapy of AIDS</ce:text></ce:keyword><ce:keyword><ce:text>Phosphorothioate</ce:text></ce:keyword><ce:keyword><ce:text>Methylphosphonate</ce:text></ce:keyword><ce:keyword><ce:text>Phosphoramidate</ce:text></ce:keyword><ce:keyword><ce:text>Inhibition of human immunodeficiency virus</ce:text></ce:keyword></ce:keywords><ce:keywords class="abr"><ce:section-title>Abbreviations</ce:section-title><ce:keyword><ce:text>AUG, initiation codon</ce:text></ce:keyword><ce:keyword><ce:text>AZT, zidovudine</ce:text></ce:keyword><ce:keyword><ce:text>DMF, dimethylformamide</ce:text></ce:keyword><ce:keyword><ce:text>Fmoc, 9-fluorenylmethoxycarbonyl</ce:text></ce:keyword><ce:keyword><ce:text>HIV, human immunodeficiency virus</ce:text></ce:keyword><ce:keyword><ce:text>nef or NEF, negative regulation factor</ce:text></ce:keyword><ce:keyword><ce:text>PBS, primer binding site</ce:text></ce:keyword><ce:keyword><ce:text>RIPA, radioimmunoprecipitation assay</ce:text></ce:keyword><ce:keyword><ce:text>TAR, transactivator responsive region</ce:text></ce:keyword><ce:keyword><ce:text>tRNA, transfer RNA</ce:text></ce:keyword><ce:keyword><ce:text>vif or VIF, viral infectivity factor</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Antisense oligonucleotides: gene regulation and chemotherapy of AIDS</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Antisense oligonucleotides: gene regulation and chemotherapy of AIDS</title></titleInfo><name type="personal"><namePart type="given">Sudhir</namePart><namePart type="family">Agrawal</namePart><affiliation>Worcester Foundation for Experimental Biology, Shrewsbury, MA, U.S.A.</affiliation><description>Correspondence: S. Agrawal, Worcester Foundation for Experimental Biology, 222 Maple Avenue, Shrewsbury, MA 01545, U.S.A.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Prem S.</namePart><namePart type="family">Sarin</namePart><affiliation>Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, MD, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="review-article" displayLabel="Review article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-L5L7X3NF-P">review-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1991</dateIssued><copyrightDate encoding="w3cdtf">1991</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: The use of antisense oligonucleotides in control of gene regulation and inhibition of virus replication is a unique approach designed to interfere with cell and virus replication, transcription and translation machinery at the molecular level. Extensive studies ave been carried out to regulate replication of human immunodeficiency virus by synthetic oligonucleotides and their several sugar phosphate backbone modified analogues. Thus far, studies have been carried out in tissue culture, and inhibition of HIV expression has been monitored by several parameters. Oligonucleotide phosphorothioates are effective in inhibiting HIV expression in tissue culture at a concentration of 1·10−7 M, which translates into a dose of approximately 0.6 mg/kg bodyweight. A non-toxic dose of 100 mg/kg, in preliminary acute toxicity experiments, represents an exploitable therapeutic window for ‘oligonucleotide therapy’.</abstract><subject><genre>Keywords</genre><topic>Antisense oligonucleotide</topic><topic>Human immunodeficiency virus</topic><topic>Chemotherapy of AIDS</topic><topic>Phosphorothioate</topic><topic>Methylphosphonate</topic><topic>Phosphoramidate</topic><topic>Inhibition of human immunodeficiency virus</topic></subject><subject><genre>Abbreviations</genre><topic>AUG, initiation codon</topic><topic>AZT, zidovudine</topic><topic>DMF, dimethylformamide</topic><topic>Fmoc, 9-fluorenylmethoxycarbonyl</topic><topic>HIV, human immunodeficiency virus</topic><topic>nef or NEF, negative regulation factor</topic><topic>PBS, primer binding site</topic><topic>RIPA, radioimmunoprecipitation assay</topic><topic>TAR, transactivator responsive region</topic><topic>tRNA, transfer RNA</topic><topic>vif or VIF, viral infectivity factor</topic></subject><relatedItem type="host"><titleInfo><title>Advanced Drug Delivery Reviews</title></titleInfo><titleInfo type="abbreviated"><title>ADR</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1991</dateIssued></originInfo><identifier type="ISSN">0169-409X</identifier><identifier type="PII">S0169-409X(00)X0101-9</identifier><part><date>1991</date><detail type="issue"><title>Modified Therapeutic Proteins </title></detail><detail type="volume"><number>6</number><caption>vol.</caption></detail><detail type="issue"><number>3</number><caption>no.</caption></detail><extent unit="issue-pages"><start>235</start><end>322</end></extent><extent unit="pages"><start>251</start><end>270</end></extent></part></relatedItem><identifier type="istex">50A1BA6B6CFCE21A80880ECCEB083AC5D2C8CFC8</identifier><identifier type="ark">ark:/67375/6H6-DR5LG84V-0</identifier><identifier type="DOI">10.1016/0169-409X(91)90020-D</identifier><identifier type="PII">0169-409X(91)90020-D</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-DR5LG84V-0/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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