Estimation of the Sulfate Content of Hydrothermal Vent Bacterial Polysaccharides by Fourier Transform Infrared Spectroscopy
Identifieur interne : 000288 ( Istex/Corpus ); précédent : 000287; suivant : 000289Estimation of the Sulfate Content of Hydrothermal Vent Bacterial Polysaccharides by Fourier Transform Infrared Spectroscopy
Auteurs : Y. Lijour ; E. Gentric ; E. Deslandes ; J. GuezennecSource :
- Analytical Biochemistry [ 0003-2697 ] ; 1994.
Abstract
Abstract: Deep-sea hydrothermal vents are characterized by unusual chemical and physical parameters, including high pressure and temperature. In this extreme environment, unusual microorganisms of biotechnological importance survive. Polymer-producing bacteria have been specifically studied for several years with the aim of demonstrating their ability to produce unusual polysaccharides in terms of physical, chemical, and biological properties. Because sulfates play an important role in the biological properties of polysaccharides, it is very important to determine their content with accuracy. Fourier transform infrared spectroscopy (FT-IR) was used for sulfate analysis and the results were compared with those of other analytical techniques. We found a good correlation between FT-IR and other analytical techniques for sulfate concentrations ranging from 2.4 to 20%. The data indicated that this technique could be used to determine the chemical composition of the polymers along with a semiquantitative estimation of the sulfate content.
Url:
DOI: 10.1006/abio.1994.1334
Links to Exploration step
ISTEX:054FAEC8B4A918AE282C2549D5CF583ED02BAB6FLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Estimation of the Sulfate Content of Hydrothermal Vent Bacterial Polysaccharides by Fourier Transform Infrared Spectroscopy</title><author><name sortKey="Lijour, Y" sort="Lijour, Y" uniqKey="Lijour Y" first="Y." last="Lijour">Y. Lijour</name><affiliation><mods:affiliation>Ifremer, Ctr Brest, Dept Dro EP Lbmh, Biotechnol Lab, F 29280 Plouzane, France; Univ Brest, Spectrochim Molec Lab, F 29285 Brest, France and Univ Brest, Ecophysiol & Biochim Algues Marines Lab, F 29285 Brest, France</mods:affiliation></affiliation></author><author><name sortKey="Gentric, E" sort="Gentric, E" uniqKey="Gentric E" first="E." last="Gentric">E. Gentric</name><affiliation><mods:affiliation>Ifremer, Ctr Brest, Dept Dro EP Lbmh, Biotechnol Lab, F 29280 Plouzane, France; Univ Brest, Spectrochim Molec Lab, F 29285 Brest, France and Univ Brest, Ecophysiol & Biochim Algues Marines Lab, F 29285 Brest, France</mods:affiliation></affiliation></author><author><name sortKey="Deslandes, E" sort="Deslandes, E" uniqKey="Deslandes E" first="E." last="Deslandes">E. Deslandes</name><affiliation><mods:affiliation>Ifremer, Ctr Brest, Dept Dro EP Lbmh, Biotechnol Lab, F 29280 Plouzane, France; Univ Brest, Spectrochim Molec Lab, F 29285 Brest, France and Univ Brest, Ecophysiol & Biochim Algues Marines Lab, F 29285 Brest, France</mods:affiliation></affiliation></author><author><name sortKey="Guezennec, J" sort="Guezennec, J" uniqKey="Guezennec J" first="J." last="Guezennec">J. Guezennec</name><affiliation><mods:affiliation>Ifremer, Ctr Brest, Dept Dro EP Lbmh, Biotechnol Lab, F 29280 Plouzane, France; Univ Brest, Spectrochim Molec Lab, F 29285 Brest, France and Univ Brest, Ecophysiol & Biochim Algues Marines Lab, F 29285 Brest, France</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:054FAEC8B4A918AE282C2549D5CF583ED02BAB6F</idno><date when="1994" year="1994">1994</date><idno type="doi">10.1006/abio.1994.1334</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-K5QHD193-M/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000288</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000288</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Estimation of the Sulfate Content of Hydrothermal Vent Bacterial Polysaccharides by Fourier Transform Infrared Spectroscopy</title><author><name sortKey="Lijour, Y" sort="Lijour, Y" uniqKey="Lijour Y" first="Y." last="Lijour">Y. Lijour</name><affiliation><mods:affiliation>Ifremer, Ctr Brest, Dept Dro EP Lbmh, Biotechnol Lab, F 29280 Plouzane, France; Univ Brest, Spectrochim Molec Lab, F 29285 Brest, France and Univ Brest, Ecophysiol & Biochim Algues Marines Lab, F 29285 Brest, France</mods:affiliation></affiliation></author><author><name sortKey="Gentric, E" sort="Gentric, E" uniqKey="Gentric E" first="E." last="Gentric">E. Gentric</name><affiliation><mods:affiliation>Ifremer, Ctr Brest, Dept Dro EP Lbmh, Biotechnol Lab, F 29280 Plouzane, France; Univ Brest, Spectrochim Molec Lab, F 29285 Brest, France and Univ Brest, Ecophysiol & Biochim Algues Marines Lab, F 29285 Brest, France</mods:affiliation></affiliation></author><author><name sortKey="Deslandes, E" sort="Deslandes, E" uniqKey="Deslandes E" first="E." last="Deslandes">E. Deslandes</name><affiliation><mods:affiliation>Ifremer, Ctr Brest, Dept Dro EP Lbmh, Biotechnol Lab, F 29280 Plouzane, France; Univ Brest, Spectrochim Molec Lab, F 29285 Brest, France and Univ Brest, Ecophysiol & Biochim Algues Marines Lab, F 29285 Brest, France</mods:affiliation></affiliation></author><author><name sortKey="Guezennec, J" sort="Guezennec, J" uniqKey="Guezennec J" first="J." last="Guezennec">J. Guezennec</name><affiliation><mods:affiliation>Ifremer, Ctr Brest, Dept Dro EP Lbmh, Biotechnol Lab, F 29280 Plouzane, France; Univ Brest, Spectrochim Molec Lab, F 29285 Brest, France and Univ Brest, Ecophysiol & Biochim Algues Marines Lab, F 29285 Brest, France</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Analytical Biochemistry</title><title level="j" type="abbrev">YABIO</title><idno type="ISSN">0003-2697</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994">1994</date><biblScope unit="volume">220</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="244">244</biblScope><biblScope unit="page" to="248">248</biblScope></imprint><idno type="ISSN">0003-2697</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0003-2697</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Deep-sea hydrothermal vents are characterized by unusual chemical and physical parameters, including high pressure and temperature. In this extreme environment, unusual microorganisms of biotechnological importance survive. Polymer-producing bacteria have been specifically studied for several years with the aim of demonstrating their ability to produce unusual polysaccharides in terms of physical, chemical, and biological properties. Because sulfates play an important role in the biological properties of polysaccharides, it is very important to determine their content with accuracy. Fourier transform infrared spectroscopy (FT-IR) was used for sulfate analysis and the results were compared with those of other analytical techniques. We found a good correlation between FT-IR and other analytical techniques for sulfate concentrations ranging from 2.4 to 20%. The data indicated that this technique could be used to determine the chemical composition of the polymers along with a semiquantitative estimation of the sulfate content.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Y. Lijour</name><affiliations><json:string>Ifremer, Ctr Brest, Dept Dro EP Lbmh, Biotechnol Lab, F 29280 Plouzane, France; Univ Brest, Spectrochim Molec Lab, F 29285 Brest, France and Univ Brest, Ecophysiol & Biochim Algues Marines Lab, F 29285 Brest, France</json:string></affiliations></json:item><json:item><name>E. Gentric</name><affiliations><json:string>Ifremer, Ctr Brest, Dept Dro EP Lbmh, Biotechnol Lab, F 29280 Plouzane, France; Univ Brest, Spectrochim Molec Lab, F 29285 Brest, France and Univ Brest, Ecophysiol & Biochim Algues Marines Lab, F 29285 Brest, France</json:string></affiliations></json:item><json:item><name>E. Deslandes</name><affiliations><json:string>Ifremer, Ctr Brest, Dept Dro EP Lbmh, Biotechnol Lab, F 29280 Plouzane, France; Univ Brest, Spectrochim Molec Lab, F 29285 Brest, France and Univ Brest, Ecophysiol & Biochim Algues Marines Lab, F 29285 Brest, France</json:string></affiliations></json:item><json:item><name>J. Guezennec</name><affiliations><json:string>Ifremer, Ctr Brest, Dept Dro EP Lbmh, Biotechnol Lab, F 29280 Plouzane, France; Univ Brest, Spectrochim Molec Lab, F 29285 Brest, France and Univ Brest, Ecophysiol & Biochim Algues Marines Lab, F 29285 Brest, France</json:string></affiliations></json:item></author><articleId><json:string>71334</json:string></articleId><arkIstex>ark:/67375/6H6-K5QHD193-M</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: Deep-sea hydrothermal vents are characterized by unusual chemical and physical parameters, including high pressure and temperature. In this extreme environment, unusual microorganisms of biotechnological importance survive. Polymer-producing bacteria have been specifically studied for several years with the aim of demonstrating their ability to produce unusual polysaccharides in terms of physical, chemical, and biological properties. Because sulfates play an important role in the biological properties of polysaccharides, it is very important to determine their content with accuracy. Fourier transform infrared spectroscopy (FT-IR) was used for sulfate analysis and the results were compared with those of other analytical techniques. We found a good correlation between FT-IR and other analytical techniques for sulfate concentrations ranging from 2.4 to 20%. The data indicated that this technique could be used to determine the chemical composition of the polymers along with a semiquantitative estimation of the sulfate content.</abstract><qualityIndicators><score>3.766</score><pdfWordCount>0</pdfWordCount><pdfCharCount>0</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>5</pdfPageCount><pdfPageSize>566 x 775 pts</pdfPageSize><refBibsNative>false</refBibsNative><abstractWordCount>143</abstractWordCount><abstractCharCount>1048</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Estimation of the Sulfate Content of Hydrothermal Vent Bacterial Polysaccharides by Fourier Transform Infrared Spectroscopy</title><pmid><json:string>7978265</json:string></pmid><pii><json:string>S0003-2697(84)71334-0</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>Analytical Biochemistry</title><language><json:string>unknown</json:string></language><publicationDate>1994</publicationDate><issn><json:string>0003-2697</json:string></issn><pii><json:string>S0003-2697(00)X0162-5</json:string></pii><volume>220</volume><issue>2</issue><pages><first>244</first><last>248</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date></date><geogName></geogName><orgName></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName></persName><placeName></placeName><ref_url></ref_url><ref_bibl></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-K5QHD193-M</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - chemistry, analytical</json:string><json:string>2 - biochemistry & molecular biology</json:string><json:string>2 - biochemical research methods</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - biochemistry & molecular biology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Cell Biology</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Molecular Biology</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Biochemistry</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Biophysics</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string></inist></categories><publicationDate>1994</publicationDate><copyrightDate>1994</copyrightDate><doi><json:string>10.1006/abio.1994.1334</json:string></doi><id>054FAEC8B4A918AE282C2549D5CF583ED02BAB6F</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-K5QHD193-M/fulltext.pdf</uri></json:item><json:item><extension>ocr</extension><original>false</original><mimetype>text/ocr</mimetype><quality><totalToken>3376</totalToken><correct>3066</correct><rate>80.13583181686707</rate><misspelled>308</misspelled></quality><langDetect><reliable>true</reliable><languages><json:item><score>927</score><code>en</code><name>ENGLISH</name><percent>97</percent></json:item><json:item><score>703</score><code>fr</code><name>FRENCH</name><percent>0</percent></json:item></languages></langDetect><uri>https://api.istex.fr/ark:/67375/6H6-K5QHD193-M/fulltext.ocr</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-K5QHD193-M/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-K5QHD193-M/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Estimation of the Sulfate Content of Hydrothermal Vent Bacterial Polysaccharides by Fourier Transform Infrared Spectroscopy</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>©1994 Academic Press</p></licence><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</p></availability><date>1994</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note type="content">Section title: Regular Article</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Estimation of the Sulfate Content of Hydrothermal Vent Bacterial Polysaccharides by Fourier Transform Infrared Spectroscopy</title><author xml:id="author-0000"><persName><forename type="first">Y.</forename><surname>Lijour</surname></persName><affiliation>Ifremer, Ctr Brest, Dept Dro EP Lbmh, Biotechnol Lab, F 29280 Plouzane, France; Univ Brest, Spectrochim Molec Lab, F 29285 Brest, France and Univ Brest, Ecophysiol & Biochim Algues Marines Lab, F 29285 Brest, France</affiliation></author><author xml:id="author-0001"><persName><forename type="first">E.</forename><surname>Gentric</surname></persName><affiliation>Ifremer, Ctr Brest, Dept Dro EP Lbmh, Biotechnol Lab, F 29280 Plouzane, France; Univ Brest, Spectrochim Molec Lab, F 29285 Brest, France and Univ Brest, Ecophysiol & Biochim Algues Marines Lab, F 29285 Brest, France</affiliation></author><author xml:id="author-0002"><persName><forename type="first">E.</forename><surname>Deslandes</surname></persName><affiliation>Ifremer, Ctr Brest, Dept Dro EP Lbmh, Biotechnol Lab, F 29280 Plouzane, France; Univ Brest, Spectrochim Molec Lab, F 29285 Brest, France and Univ Brest, Ecophysiol & Biochim Algues Marines Lab, F 29285 Brest, France</affiliation></author><author xml:id="author-0003"><persName><forename type="first">J.</forename><surname>Guezennec</surname></persName><affiliation>Ifremer, Ctr Brest, Dept Dro EP Lbmh, Biotechnol Lab, F 29280 Plouzane, France; Univ Brest, Spectrochim Molec Lab, F 29285 Brest, France and Univ Brest, Ecophysiol & Biochim Algues Marines Lab, F 29285 Brest, France</affiliation></author><idno type="istex">054FAEC8B4A918AE282C2549D5CF583ED02BAB6F</idno><idno type="ark">ark:/67375/6H6-K5QHD193-M</idno><idno type="DOI">10.1006/abio.1994.1334</idno><idno type="PII">S0003-2697(84)71334-0</idno><idno type="article-id">71334</idno></analytic><monogr><title level="j">Analytical Biochemistry</title><title level="j" type="abbrev">YABIO</title><idno type="pISSN">0003-2697</idno><idno type="PII">S0003-2697(00)X0162-5</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994"></date><biblScope unit="volume">220</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="244">244</biblScope><biblScope unit="page" to="248">248</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1994</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Deep-sea hydrothermal vents are characterized by unusual chemical and physical parameters, including high pressure and temperature. In this extreme environment, unusual microorganisms of biotechnological importance survive. Polymer-producing bacteria have been specifically studied for several years with the aim of demonstrating their ability to produce unusual polysaccharides in terms of physical, chemical, and biological properties. Because sulfates play an important role in the biological properties of polysaccharides, it is very important to determine their content with accuracy. Fourier transform infrared spectroscopy (FT-IR) was used for sulfate analysis and the results were compared with those of other analytical techniques. We found a good correlation between FT-IR and other analytical techniques for sulfate concentrations ranging from 2.4 to 20%. The data indicated that this technique could be used to determine the chemical composition of the polymers along with a semiquantitative estimation of the sulfate content.</p></abstract></profileDesc><revisionDesc><change when="1994">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-K5QHD193-M/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier converted-article found"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>YABIO</jid><aid>71334</aid><ce:pii>S0003-2697(84)71334-0</ce:pii><ce:doi>10.1006/abio.1994.1334</ce:doi><ce:copyright type="full-transfer" year="1994">Academic Press</ce:copyright></item-info><head><ce:dochead><ce:textfn>Regular Article</ce:textfn></ce:dochead><ce:title>Estimation of the Sulfate Content of Hydrothermal Vent Bacterial Polysaccharides by Fourier Transform Infrared Spectroscopy</ce:title><ce:author-group><ce:author><ce:surname>Lijour</ce:surname><ce:given-name>Y.</ce:given-name></ce:author><ce:author><ce:surname>Gentric</ce:surname><ce:given-name>E.</ce:given-name></ce:author><ce:author><ce:surname>Deslandes</ce:surname><ce:given-name>E.</ce:given-name></ce:author><ce:author><ce:surname>Guezennec</ce:surname><ce:given-name>J.</ce:given-name></ce:author><ce:affiliation><ce:textfn>Ifremer, Ctr Brest, Dept Dro EP Lbmh, Biotechnol Lab, F 29280 Plouzane, France; Univ Brest, Spectrochim Molec Lab, F 29285 Brest, France and Univ Brest, Ecophysiol & Biochim Algues Marines Lab, F 29285 Brest, France</ce:textfn></ce:affiliation></ce:author-group><ce:abstract class="author"><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para view="all" id="simple-para.0010">Deep-sea hydrothermal vents are characterized by unusual chemical and physical parameters, including high pressure and temperature. In this extreme environment, unusual microorganisms of biotechnological importance survive. Polymer-producing bacteria have been specifically studied for several years with the aim of demonstrating their ability to produce unusual polysaccharides in terms of physical, chemical, and biological properties. Because sulfates play an important role in the biological properties of polysaccharides, it is very important to determine their content with accuracy. Fourier transform infrared spectroscopy (FT-IR) was used for sulfate analysis and the results were compared with those of other analytical techniques. We found a good correlation between FT-IR and other analytical techniques for sulfate concentrations ranging from 2.4 to 20%. The data indicated that this technique could be used to determine the chemical composition of the polymers along with a semiquantitative estimation of the sulfate content.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Estimation of the Sulfate Content of Hydrothermal Vent Bacterial Polysaccharides by Fourier Transform Infrared Spectroscopy</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Estimation of the Sulfate Content of Hydrothermal Vent Bacterial Polysaccharides by Fourier Transform Infrared Spectroscopy</title></titleInfo><name type="personal"><namePart type="given">Y.</namePart><namePart type="family">Lijour</namePart><affiliation>Ifremer, Ctr Brest, Dept Dro EP Lbmh, Biotechnol Lab, F 29280 Plouzane, France; Univ Brest, Spectrochim Molec Lab, F 29285 Brest, France and Univ Brest, Ecophysiol & Biochim Algues Marines Lab, F 29285 Brest, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Gentric</namePart><affiliation>Ifremer, Ctr Brest, Dept Dro EP Lbmh, Biotechnol Lab, F 29280 Plouzane, France; Univ Brest, Spectrochim Molec Lab, F 29285 Brest, France and Univ Brest, Ecophysiol & Biochim Algues Marines Lab, F 29285 Brest, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Deslandes</namePart><affiliation>Ifremer, Ctr Brest, Dept Dro EP Lbmh, Biotechnol Lab, F 29280 Plouzane, France; Univ Brest, Spectrochim Molec Lab, F 29285 Brest, France and Univ Brest, Ecophysiol & Biochim Algues Marines Lab, F 29285 Brest, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Guezennec</namePart><affiliation>Ifremer, Ctr Brest, Dept Dro EP Lbmh, Biotechnol Lab, F 29280 Plouzane, France; Univ Brest, Spectrochim Molec Lab, F 29285 Brest, France and Univ Brest, Ecophysiol & Biochim Algues Marines Lab, F 29285 Brest, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1994</dateIssued><copyrightDate encoding="w3cdtf">1994</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Deep-sea hydrothermal vents are characterized by unusual chemical and physical parameters, including high pressure and temperature. In this extreme environment, unusual microorganisms of biotechnological importance survive. Polymer-producing bacteria have been specifically studied for several years with the aim of demonstrating their ability to produce unusual polysaccharides in terms of physical, chemical, and biological properties. Because sulfates play an important role in the biological properties of polysaccharides, it is very important to determine their content with accuracy. Fourier transform infrared spectroscopy (FT-IR) was used for sulfate analysis and the results were compared with those of other analytical techniques. We found a good correlation between FT-IR and other analytical techniques for sulfate concentrations ranging from 2.4 to 20%. The data indicated that this technique could be used to determine the chemical composition of the polymers along with a semiquantitative estimation of the sulfate content.</abstract><note type="content">Section title: Regular Article</note><relatedItem type="host"><titleInfo><title>Analytical Biochemistry</title></titleInfo><titleInfo type="abbreviated"><title>YABIO</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1994</dateIssued></originInfo><identifier type="ISSN">0003-2697</identifier><identifier type="PII">S0003-2697(00)X0162-5</identifier><part><date>1994</date><detail type="volume"><number>220</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue-pages"><start>227</start><end>440</end></extent><extent unit="pages"><start>244</start><end>248</end></extent></part></relatedItem><identifier type="istex">054FAEC8B4A918AE282C2549D5CF583ED02BAB6F</identifier><identifier type="ark">ark:/67375/6H6-K5QHD193-M</identifier><identifier type="DOI">10.1006/abio.1994.1334</identifier><identifier type="PII">S0003-2697(84)71334-0</identifier><identifier type="ArticleID">71334</identifier><accessCondition type="use and reproduction" contentType="copyright">©1994 Academic Press</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource><recordOrigin>Academic Press, ©1994</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-K5QHD193-M/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000288 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000288 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:054FAEC8B4A918AE282C2549D5CF583ED02BAB6F
|texte= Estimation of the Sulfate Content of Hydrothermal Vent Bacterial Polysaccharides by Fourier Transform Infrared Spectroscopy
}}
| This area was generated with Dilib version V0.6.33. |  |