Phage displayed 6-mer mimotopes with a consensus proline absent in the minimized linear wild-type epitope
Identifieur interne : 000198 ( Istex/Corpus ); précédent : 000197; suivant : 000199Phage displayed 6-mer mimotopes with a consensus proline absent in the minimized linear wild-type epitope
Auteurs : Sabrina Deroo ; Philippe Fournier ; Dietmar Theisen ; Nicolas H. C. Brons ; Hans Deckmyn ; Claude P. MullerSource :
- Letters in Peptide Science [ 0929-5666 ] ; 1998-05-01.
English descriptors
Abstract
Abstract: Phage displayed random 6-mer libraries were screened with a monoclonal antibody specific for a minimized ‘linear’ 7-mer epitope of the measles virus hemagglutinin protein. No clone with the wild-type sequence was selected and most clones contained a sequence motif not found in the wild-type sequence. Two mimotopes (LYMPQLS, SEMPQLP) were synthesized which inhibited binding to the measles virus 95–135 times better than a wild-type peptide. Sequence comparison of proteins with known 3D-structure indicates that the epitope corresponds to an α-helix, while the best mimotopes have no predicted helix propensity. The proline is thought to be required for inducing a turn necessary for mimicking part of the α-helix. The higher intrinsic stability of such a mimotope may explain its improved binding and may be more suitable in immunogenicity experiments.
Url:
DOI: 10.1023/A:1008893218958
Links to Exploration step
ISTEX:D88406C219295F9B543D7A8FB2E4AA19AABEF3D5Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Phage displayed 6-mer mimotopes with a consensus proline absent in the minimized linear wild-type epitope</title><author><name sortKey="Deroo, Sabrina" sort="Deroo, Sabrina" uniqKey="Deroo S" first="Sabrina" last="Deroo">Sabrina Deroo</name><affiliation><mods:affiliation>Laboratoire National de Santé, P.O. Box 1102, L-1011, Luxembourg, Luxembourg</mods:affiliation></affiliation></author><author><name sortKey="Fournier, Philippe" sort="Fournier, Philippe" uniqKey="Fournier P" first="Philippe" last="Fournier">Philippe Fournier</name><affiliation><mods:affiliation>Laboratoire National de Santé, P.O. Box 1102, L-1011, Luxembourg, Luxembourg</mods:affiliation></affiliation></author><author><name sortKey="Theisen, Dietmar" sort="Theisen, Dietmar" uniqKey="Theisen D" first="Dietmar" last="Theisen">Dietmar Theisen</name><affiliation><mods:affiliation>Laboratoire National de Santé, P.O. Box 1102, L-1011, Luxembourg, Luxembourg</mods:affiliation></affiliation></author><author><name sortKey="Brons, Nicolas H C" sort="Brons, Nicolas H C" uniqKey="Brons N" first="Nicolas H. C." last="Brons">Nicolas H. C. Brons</name><affiliation><mods:affiliation>Laboratoire National de Santé, P.O. Box 1102, L-1011, Luxembourg, Luxembourg</mods:affiliation></affiliation></author><author><name sortKey="Deckmyn, Hans" sort="Deckmyn, Hans" uniqKey="Deckmyn H" first="Hans" last="Deckmyn">Hans Deckmyn</name><affiliation><mods:affiliation>Laboratory for Thrombosis Research, Interdisciplinary Research Center, KU Leuven Campus Kortrijk, B-8500, Kortrijk, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Muller, Claude P" sort="Muller, Claude P" uniqKey="Muller C" first="Claude P." last="Muller">Claude P. Muller</name><affiliation><mods:affiliation>Laboratoire National de Santé, P.O. Box 1102, L-1011, Luxembourg, Luxembourg</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:D88406C219295F9B543D7A8FB2E4AA19AABEF3D5</idno><date when="1998" year="1998">1998</date><idno type="doi">10.1023/A:1008893218958</idno><idno type="url">https://api.istex.fr/ark:/67375/VQC-JZ08CJ3Q-4/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000198</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000198</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Phage displayed 6-mer mimotopes with a consensus proline absent in the minimized linear wild-type epitope</title><author><name sortKey="Deroo, Sabrina" sort="Deroo, Sabrina" uniqKey="Deroo S" first="Sabrina" last="Deroo">Sabrina Deroo</name><affiliation><mods:affiliation>Laboratoire National de Santé, P.O. Box 1102, L-1011, Luxembourg, Luxembourg</mods:affiliation></affiliation></author><author><name sortKey="Fournier, Philippe" sort="Fournier, Philippe" uniqKey="Fournier P" first="Philippe" last="Fournier">Philippe Fournier</name><affiliation><mods:affiliation>Laboratoire National de Santé, P.O. Box 1102, L-1011, Luxembourg, Luxembourg</mods:affiliation></affiliation></author><author><name sortKey="Theisen, Dietmar" sort="Theisen, Dietmar" uniqKey="Theisen D" first="Dietmar" last="Theisen">Dietmar Theisen</name><affiliation><mods:affiliation>Laboratoire National de Santé, P.O. Box 1102, L-1011, Luxembourg, Luxembourg</mods:affiliation></affiliation></author><author><name sortKey="Brons, Nicolas H C" sort="Brons, Nicolas H C" uniqKey="Brons N" first="Nicolas H. C." last="Brons">Nicolas H. C. Brons</name><affiliation><mods:affiliation>Laboratoire National de Santé, P.O. Box 1102, L-1011, Luxembourg, Luxembourg</mods:affiliation></affiliation></author><author><name sortKey="Deckmyn, Hans" sort="Deckmyn, Hans" uniqKey="Deckmyn H" first="Hans" last="Deckmyn">Hans Deckmyn</name><affiliation><mods:affiliation>Laboratory for Thrombosis Research, Interdisciplinary Research Center, KU Leuven Campus Kortrijk, B-8500, Kortrijk, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Muller, Claude P" sort="Muller, Claude P" uniqKey="Muller C" first="Claude P." last="Muller">Claude P. Muller</name><affiliation><mods:affiliation>Laboratoire National de Santé, P.O. Box 1102, L-1011, Luxembourg, Luxembourg</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Letters in Peptide Science</title><title level="j" type="abbrev">Letters in Peptide Science</title><idno type="ISSN">0929-5666</idno><idno type="eISSN">1573-496X</idno><imprint><publisher>Kluwer Academic Publishers</publisher><pubPlace>Dordrecht</pubPlace><date type="published" when="1998-05-01">1998-05-01</date><biblScope unit="volume">5</biblScope><biblScope unit="issue">2-3</biblScope><biblScope unit="page" from="159">159</biblScope><biblScope unit="page" to="162">162</biblScope></imprint><idno type="ISSN">0929-5666</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0929-5666</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>measles virus</term><term>monoclonal antibody</term><term>peptides</term><term>phage display libraries</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Phage displayed random 6-mer libraries were screened with a monoclonal antibody specific for a minimized ‘linear’ 7-mer epitope of the measles virus hemagglutinin protein. No clone with the wild-type sequence was selected and most clones contained a sequence motif not found in the wild-type sequence. Two mimotopes (LYMPQLS, SEMPQLP) were synthesized which inhibited binding to the measles virus 95–135 times better than a wild-type peptide. Sequence comparison of proteins with known 3D-structure indicates that the epitope corresponds to an α-helix, while the best mimotopes have no predicted helix propensity. The proline is thought to be required for inducing a turn necessary for mimicking part of the α-helix. The higher intrinsic stability of such a mimotope may explain its improved binding and may be more suitable in immunogenicity experiments.</div></front></TEI><istex><corpusName>springer-journals</corpusName><author><json:item><name>Sabrina Deroo</name><affiliations><json:string>Laboratoire National de Santé, P.O. Box 1102, L-1011, Luxembourg, Luxembourg</json:string></affiliations></json:item><json:item><name>Philippe Fournier</name><affiliations><json:string>Laboratoire National de Santé, P.O. Box 1102, L-1011, Luxembourg, Luxembourg</json:string></affiliations></json:item><json:item><name>Dietmar Theisen</name><affiliations><json:string>Laboratoire National de Santé, P.O. Box 1102, L-1011, Luxembourg, Luxembourg</json:string></affiliations></json:item><json:item><name>Nicolas H.C. Brons</name><affiliations><json:string>Laboratoire National de Santé, P.O. Box 1102, L-1011, Luxembourg, Luxembourg</json:string></affiliations></json:item><json:item><name>Hans Deckmyn</name><affiliations><json:string>Laboratory for Thrombosis Research, Interdisciplinary Research Center, KU Leuven Campus Kortrijk, B-8500, Kortrijk, Belgium</json:string></affiliations></json:item><json:item><name>Claude P. Muller</name><affiliations><json:string>Laboratoire National de Santé, P.O. Box 1102, L-1011, Luxembourg, Luxembourg</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>measles virus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>monoclonal antibody</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>peptides</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>phage display libraries</value></json:item></subject><articleId><json:string>146100</json:string><json:string>Art24</json:string></articleId><arkIstex>ark:/67375/VQC-JZ08CJ3Q-4</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Abstract: Phage displayed random 6-mer libraries were screened with a monoclonal antibody specific for a minimized ‘linear’ 7-mer epitope of the measles virus hemagglutinin protein. No clone with the wild-type sequence was selected and most clones contained a sequence motif not found in the wild-type sequence. Two mimotopes (LYMPQLS, SEMPQLP) were synthesized which inhibited binding to the measles virus 95–135 times better than a wild-type peptide. Sequence comparison of proteins with known 3D-structure indicates that the epitope corresponds to an α-helix, while the best mimotopes have no predicted helix propensity. The proline is thought to be required for inducing a turn necessary for mimicking part of the α-helix. The higher intrinsic stability of such a mimotope may explain its improved binding and may be more suitable in immunogenicity experiments.</abstract><qualityIndicators><score>4.603</score><pdfWordCount>1055</pdfWordCount><pdfCharCount>6583</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>4</pdfPageCount><pdfPageSize>595 x 742 pts</pdfPageSize><refBibsNative>false</refBibsNative><abstractWordCount>129</abstractWordCount><abstractCharCount>865</abstractCharCount><keywordCount>4</keywordCount></qualityIndicators><title>Phage displayed 6-mer mimotopes with a consensus proline absent in the minimized linear wild-type epitope</title><genre><json:string>research-article</json:string></genre><host><title>Letters in Peptide Science</title><language><json:string>unknown</json:string></language><publicationDate>1998</publicationDate><copyrightDate>1998</copyrightDate><issn><json:string>0929-5666</json:string></issn><eissn><json:string>1573-496X</json:string></eissn><journalId><json:string>10989</json:string></journalId><volume>5</volume><issue>2-3</issue><pages><first>159</first><last>162</last></pages><genre><json:string>journal</json:string></genre><subject><json:item><value>Polymer Sciences</value></json:item><json:item><value>Biochemistry, general</value></json:item><json:item><value>Animal Anatomy / Morphology / Histology</value></json:item></subject></host><ark><json:string>ark:/67375/VQC-JZ08CJ3Q-4</json:string></ark><publicationDate>1998</publicationDate><copyrightDate>1998</copyrightDate><doi><json:string>10.1023/A:1008893218958</json:string></doi><id>D88406C219295F9B543D7A8FB2E4AA19AABEF3D5</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-JZ08CJ3Q-4/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-JZ08CJ3Q-4/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/VQC-JZ08CJ3Q-4/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Phage displayed 6-mer mimotopes with a consensus proline absent in the minimized linear wild-type epitope</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">Kluwer Academic Publishers</publisher><pubPlace>Dordrecht</pubPlace><availability><licence><p>Kluwer Academic Publishers, 1998</p></licence><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</p></availability><date>1998</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Phage displayed 6-mer mimotopes with a consensus proline absent in the minimized linear wild-type epitope</title><author xml:id="author-0000"><persName><forename type="first">Sabrina</forename><surname>Deroo</surname></persName><affiliation>Laboratoire National de Santé, P.O. Box 1102, L-1011, Luxembourg, Luxembourg</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Philippe</forename><surname>Fournier</surname></persName><affiliation>Laboratoire National de Santé, P.O. Box 1102, L-1011, Luxembourg, Luxembourg</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Dietmar</forename><surname>Theisen</surname></persName><affiliation>Laboratoire National de Santé, P.O. Box 1102, L-1011, Luxembourg, Luxembourg</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Nicolas</forename><surname>Brons</surname></persName><affiliation>Laboratoire National de Santé, P.O. Box 1102, L-1011, Luxembourg, Luxembourg</affiliation></author><author xml:id="author-0004"><persName><forename type="first">Hans</forename><surname>Deckmyn</surname></persName><affiliation>Laboratory for Thrombosis Research, Interdisciplinary Research Center, KU Leuven Campus Kortrijk, B-8500, Kortrijk, Belgium</affiliation></author><author xml:id="author-0005" corresp="yes"><persName><forename type="first">Claude</forename><surname>Muller</surname></persName><affiliation>Laboratoire National de Santé, P.O. Box 1102, L-1011, Luxembourg, Luxembourg</affiliation></author><idno type="istex">D88406C219295F9B543D7A8FB2E4AA19AABEF3D5</idno><idno type="ark">ark:/67375/VQC-JZ08CJ3Q-4</idno><idno type="DOI">10.1023/A:1008893218958</idno><idno type="article-id">146100</idno><idno type="article-id">Art24</idno></analytic><monogr><title level="j">Letters in Peptide Science</title><title level="j" type="abbrev">Letters in Peptide Science</title><idno type="pISSN">0929-5666</idno><idno type="eISSN">1573-496X</idno><idno type="journal-ID">true</idno><idno type="issue-article-count">39</idno><idno type="volume-issue-count">6</idno><imprint><publisher>Kluwer Academic Publishers</publisher><pubPlace>Dordrecht</pubPlace><date type="published" when="1998-05-01"></date><biblScope unit="volume">5</biblScope><biblScope unit="issue">2-3</biblScope><biblScope unit="page" from="159">159</biblScope><biblScope unit="page" to="162">162</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1998</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Phage displayed random 6-mer libraries were screened with a monoclonal antibody specific for a minimized ‘linear’ 7-mer epitope of the measles virus hemagglutinin protein. No clone with the wild-type sequence was selected and most clones contained a sequence motif not found in the wild-type sequence. Two mimotopes (LYMPQLS, SEMPQLP) were synthesized which inhibited binding to the measles virus 95–135 times better than a wild-type peptide. Sequence comparison of proteins with known 3D-structure indicates that the epitope corresponds to an α-helix, while the best mimotopes have no predicted helix propensity. The proline is thought to be required for inducing a turn necessary for mimicking part of the α-helix. The higher intrinsic stability of such a mimotope may explain its improved binding and may be more suitable in immunogenicity experiments.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><item><term>measles virus</term></item><item><term>monoclonal antibody</term></item><item><term>peptides</term></item><item><term>phage display libraries</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>Life Sciences</head><item><term>Polymer Sciences</term></item><item><term>Biochemistry, general</term></item><item><term>Animal Anatomy / Morphology / Histology</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1998-05-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-JZ08CJ3Q-4/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus springer-journals not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" name="istex:docType"></istex:docType><istex:document><Publisher><PublisherInfo><PublisherName>Kluwer Academic Publishers</PublisherName><PublisherLocation>Dordrecht</PublisherLocation></PublisherInfo><Journal><JournalInfo JournalProductType="ArchiveJournal" NumberingStyle="Unnumbered"><JournalID>10989</JournalID><JournalPrintISSN>0929-5666</JournalPrintISSN><JournalElectronicISSN>1573-496X</JournalElectronicISSN><JournalTitle>Letters in Peptide Science</JournalTitle><JournalAbbreviatedTitle>Letters in Peptide Science</JournalAbbreviatedTitle><JournalSubjectGroup><JournalSubject Type="Primary">Life Sciences</JournalSubject><JournalSubject Type="Secondary">Polymer Sciences</JournalSubject><JournalSubject Type="Secondary">Biochemistry, general</JournalSubject><JournalSubject Type="Secondary">Animal Anatomy / Morphology / Histology</JournalSubject></JournalSubjectGroup></JournalInfo><Volume><VolumeInfo VolumeType="Regular" TocLevels="0"><VolumeIDStart>5</VolumeIDStart><VolumeIDEnd>5</VolumeIDEnd><VolumeIssueCount>6</VolumeIssueCount></VolumeInfo><Issue IssueType="Combined"><IssueInfo TocLevels="0"><IssueIDStart>2</IssueIDStart><IssueIDEnd>3</IssueIDEnd><IssueArticleCount>39</IssueArticleCount><IssueHistory><CoverDate><Year>1998</Year><Month>5</Month></CoverDate></IssueHistory><IssueCopyright><CopyrightHolderName>Kluwer Academic Publishers</CopyrightHolderName><CopyrightYear>1998</CopyrightYear></IssueCopyright></IssueInfo><Article ID="Art24"><ArticleInfo Language="En" ArticleType="OriginalPaper" NumberingStyle="Unnumbered" TocLevels="0" ContainsESM="No"><ArticleID>146100</ArticleID><ArticleDOI>10.1023/A:1008893218958</ArticleDOI><ArticleSequenceNumber>24</ArticleSequenceNumber><ArticleTitle Language="En">Phage displayed 6-mer mimotopes with a consensus proline absent in the minimized linear wild-type epitope</ArticleTitle><ArticleFirstPage>159</ArticleFirstPage><ArticleLastPage>162</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2004</Year><Month>10</Month><Day>15</Day></RegistrationDate></ArticleHistory><ArticleCopyright><CopyrightHolderName>Kluwer Academic Publishers</CopyrightHolderName><CopyrightYear>1998</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants><ArticleContext><JournalID>10989</JournalID><VolumeIDStart>5</VolumeIDStart><VolumeIDEnd>5</VolumeIDEnd><IssueIDStart>2</IssueIDStart><IssueIDEnd>3</IssueIDEnd></ArticleContext></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Sabrina</GivenName><FamilyName>Deroo</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Philippe</GivenName><FamilyName>Fournier</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Dietmar</GivenName><FamilyName>Theisen</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Nicolas</GivenName><GivenName>H.C.</GivenName><FamilyName>Brons</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Hans</GivenName><FamilyName>Deckmyn</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Claude</GivenName><GivenName>P.</GivenName><FamilyName>Muller</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgName>Laboratoire National de Santé</OrgName><OrgAddress><Postbox>P.O. Box 1102</Postbox><Postcode>L-1011</Postcode><City>Luxembourg</City><Country>Luxembourg</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgName>Laboratory for Thrombosis Research, Interdisciplinary Research Center</OrgName><OrgAddress><Street>KU Leuven Campus Kortrijk</Street><Postcode>B-8500</Postcode><City>Kortrijk</City><Country>Belgium</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading><Para>Phage displayed random 6-mer libraries were screened with a monoclonal antibody specific for a minimized ‘linear’ 7-mer epitope of the measles virus hemagglutinin protein. No clone with the wild-type sequence was selected and most clones contained a sequence motif not found in the wild-type sequence. Two mimotopes (LYMPQLS, SEMPQLP) were synthesized which inhibited binding to the measles virus 95–135 times better than a wild-type peptide. Sequence comparison of proteins with known 3D-structure indicates that the epitope corresponds to an α-helix, while the best mimotopes have no predicted helix propensity. The proline is thought to be required for inducing a turn necessary for mimicking part of the α-helix. The higher intrinsic stability of such a mimotope may explain its improved binding and may be more suitable in immunogenicity experiments.</Para></Abstract><KeywordGroup Language="En"><Keyword>measles virus</Keyword><Keyword>monoclonal antibody</Keyword><Keyword>peptides</Keyword><Keyword>phage display libraries</Keyword></KeywordGroup></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Phage displayed 6-mer mimotopes with a consensus proline absent in the minimized linear wild-type epitope</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Phage displayed 6-mer mimotopes with a consensus proline absent in the minimized linear wild-type epitope</title></titleInfo><name type="personal"><namePart type="given">Sabrina</namePart><namePart type="family">Deroo</namePart><affiliation>Laboratoire National de Santé, P.O. Box 1102, L-1011, Luxembourg, Luxembourg</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Philippe</namePart><namePart type="family">Fournier</namePart><affiliation>Laboratoire National de Santé, P.O. Box 1102, L-1011, Luxembourg, Luxembourg</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Dietmar</namePart><namePart type="family">Theisen</namePart><affiliation>Laboratoire National de Santé, P.O. Box 1102, L-1011, Luxembourg, Luxembourg</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Nicolas</namePart><namePart type="given">H.C.</namePart><namePart type="family">Brons</namePart><affiliation>Laboratoire National de Santé, P.O. Box 1102, L-1011, Luxembourg, Luxembourg</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Hans</namePart><namePart type="family">Deckmyn</namePart><affiliation>Laboratory for Thrombosis Research, Interdisciplinary Research Center, KU Leuven Campus Kortrijk, B-8500, Kortrijk, Belgium</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Claude</namePart><namePart type="given">P.</namePart><namePart type="family">Muller</namePart><affiliation>Laboratoire National de Santé, P.O. Box 1102, L-1011, Luxembourg, Luxembourg</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Kluwer Academic Publishers</publisher><place><placeTerm type="text">Dordrecht</placeTerm></place><dateIssued encoding="w3cdtf">1998-05-01</dateIssued><copyrightDate encoding="w3cdtf">1998</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: Phage displayed random 6-mer libraries were screened with a monoclonal antibody specific for a minimized ‘linear’ 7-mer epitope of the measles virus hemagglutinin protein. No clone with the wild-type sequence was selected and most clones contained a sequence motif not found in the wild-type sequence. Two mimotopes (LYMPQLS, SEMPQLP) were synthesized which inhibited binding to the measles virus 95–135 times better than a wild-type peptide. Sequence comparison of proteins with known 3D-structure indicates that the epitope corresponds to an α-helix, while the best mimotopes have no predicted helix propensity. The proline is thought to be required for inducing a turn necessary for mimicking part of the α-helix. The higher intrinsic stability of such a mimotope may explain its improved binding and may be more suitable in immunogenicity experiments.</abstract><subject lang="en"><topic>measles virus</topic><topic>monoclonal antibody</topic><topic>peptides</topic><topic>phage display libraries</topic></subject><relatedItem type="host"><titleInfo><title>Letters in Peptide Science</title></titleInfo><titleInfo type="abbreviated"><title>Letters in Peptide Science</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">1998-05-01</dateIssued><copyrightDate encoding="w3cdtf">1998</copyrightDate></originInfo><subject><genre>Life Sciences</genre><topic>Polymer Sciences</topic><topic>Biochemistry, general</topic><topic>Animal Anatomy / Morphology / Histology</topic></subject><identifier type="ISSN">0929-5666</identifier><identifier type="eISSN">1573-496X</identifier><identifier type="JournalID">10989</identifier><identifier type="IssueArticleCount">39</identifier><identifier type="VolumeIssueCount">6</identifier><part><date>1998</date><detail type="volume"><number>5</number><caption>vol.</caption></detail><detail type="issue"><number>2-3</number><caption>no.</caption></detail><extent unit="pages"><start>159</start><end>162</end></extent></part><recordInfo><recordOrigin>Kluwer Academic Publishers, 1998</recordOrigin></recordInfo></relatedItem><identifier type="istex">D88406C219295F9B543D7A8FB2E4AA19AABEF3D5</identifier><identifier type="ark">ark:/67375/VQC-JZ08CJ3Q-4</identifier><identifier type="DOI">10.1023/A:1008893218958</identifier><identifier type="ArticleID">146100</identifier><identifier type="ArticleID">Art24</identifier><accessCondition type="use and reproduction" contentType="copyright">Kluwer Academic Publishers, 1998</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Kluwer Academic Publishers, 1998</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-JZ08CJ3Q-4/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000198 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000198 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:D88406C219295F9B543D7A8FB2E4AA19AABEF3D5
|texte= Phage displayed 6-mer mimotopes with a consensus proline absent in the minimized linear wild-type epitope
}}
| This area was generated with Dilib version V0.6.33. |  |