Polyribosomes in protoplasts isolated from tobacco leaves
Identifieur interne : 000101 ( Istex/Corpus ); précédent : 000100; suivant : 000102Polyribosomes in protoplasts isolated from tobacco leaves
Auteurs : P. Ruzicska ; R. Mettrie ; Y. L. Dorokhov ; G. Premecz ; T. Oláh ; G. L. FarkasSource :
- Planta [ 0032-0935 ] ; 1979-01-01.
English descriptors
- KwdEn :
- Teeft :
- Callus, Extraction medium, Farkas, Larkins, Leaf tissue, Leaf tissues, Osmotic stress, Physiol, Plant physiol, Plant protoplasts, Polyribosomes, Polysome, Polysome classes, Polysome preparations, Polysome profiles, Polysomes, Premecz, Protein synthesis, Protoplast, Ribosomal subunits, Rnase, Sucrose density gradient profiles, Tobacco leaf protoplasts, Tobacco protoplasts.
Abstract
Abstract: Polyribosomes (polysomes), active in an amino acid incorporation system in vitro, were isolated from tobacco leaf protoplasts. A comparison of polysome profiles indicated that the polysome/monosome ratio is greatly decreased in isolated protoplasts as compared to the intact leaf. In isolated protoplasts, a marked accumulation of ribosomal subunits was also found. The division of protoplasts, as investigated in the 8-cell and callus stages, was associated with a(n) (at least) partial regeneration of polysome profiles characteristic for leaves. Plasmolysis of leaves attached to the plant had no great effect on the polysome profile. However, leaf excision per se resulted in a dramatic loss of polysomes, even when the leaf tissue was floated on water. It is concluded that the isolation of the cell from its normal environment, and not the osmotic stress and associated increase in RNase activity, is the most important factor responsible for the loss of polysomes in isolated protoplasts.
Url:
DOI: 10.1007/BF00388718
Links to Exploration step
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<front><div type="abstract" xml:lang="en">Abstract: Polyribosomes (polysomes), active in an amino acid incorporation system in vitro, were isolated from tobacco leaf protoplasts. A comparison of polysome profiles indicated that the polysome/monosome ratio is greatly decreased in isolated protoplasts as compared to the intact leaf. In isolated protoplasts, a marked accumulation of ribosomal subunits was also found. The division of protoplasts, as investigated in the 8-cell and callus stages, was associated with a(n) (at least) partial regeneration of polysome profiles characteristic for leaves. Plasmolysis of leaves attached to the plant had no great effect on the polysome profile. However, leaf excision per se resulted in a dramatic loss of polysomes, even when the leaf tissue was floated on water. It is concluded that the isolation of the cell from its normal environment, and not the osmotic stress and associated increase in RNase activity, is the most important factor responsible for the loss of polysomes in isolated protoplasts.</div>
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<abstract xml:lang="en"><p>Abstract: Polyribosomes (polysomes), active in an amino acid incorporation system in vitro, were isolated from tobacco leaf protoplasts. A comparison of polysome profiles indicated that the polysome/monosome ratio is greatly decreased in isolated protoplasts as compared to the intact leaf. In isolated protoplasts, a marked accumulation of ribosomal subunits was also found. The division of protoplasts, as investigated in the 8-cell and callus stages, was associated with a(n) (at least) partial regeneration of polysome profiles characteristic for leaves. Plasmolysis of leaves attached to the plant had no great effect on the polysome profile. However, leaf excision per se resulted in a dramatic loss of polysomes, even when the leaf tissue was floated on water. It is concluded that the isolation of the cell from its normal environment, and not the osmotic stress and associated increase in RNase activity, is the most important factor responsible for the loss of polysomes in isolated protoplasts.</p>
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<ArticleTitle Language="En">Polyribosomes in protoplasts isolated from tobacco leaves</ArticleTitle>
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<City>Szeged</City>
<Country>Hungary</Country>
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<Affiliation ID="Aff2"><OrgDivision>Department of Cell Biology</OrgDivision>
<OrgName>University of Antwerp</OrgName>
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<Postcode>B-2610</Postcode>
<City>Wilrijk</City>
<Country>Belgium</Country>
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<Para>Polyribosomes (polysomes), active in an amino acid incorporation system in vitro, were isolated from tobacco leaf protoplasts. A comparison of polysome profiles indicated that the polysome/monosome ratio is greatly decreased in isolated protoplasts as compared to the intact leaf. In isolated protoplasts, a marked accumulation of ribosomal subunits was also found. The division of protoplasts, as investigated in the 8-cell and callus stages, was associated with a(n) (at least) partial regeneration of polysome profiles characteristic for leaves. Plasmolysis of leaves attached to the plant had no great effect on the polysome profile. However, leaf excision per se resulted in a dramatic loss of polysomes, even when the leaf tissue was floated on water. It is concluded that the isolation of the cell from its normal environment, and not the osmotic stress and associated increase in RNase activity, is the most important factor responsible for the loss of polysomes in isolated protoplasts.</Para>
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<Keyword>Protoplasts</Keyword>
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<Description><Para>ethylene glycol bis (2-aminoethyl ether)-tetraacetic acid</Para>
</Description>
</DefinitionListEntry>
<DefinitionListEntry><Term>HEPES</Term>
<Description><Para>N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid</Para>
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</DefinitionListEntry>
<DefinitionListEntry><Term>mRNA</Term>
<Description><Para>messenger ribonucleic acid</Para>
</Description>
</DefinitionListEntry>
<DefinitionListEntry><Term>RNase</Term>
<Description><Para>ribonuclease</Para>
</Description>
</DefinitionListEntry>
<DefinitionListEntry><Term>Tris</Term>
<Description><Para>tris(hydroxymethyl)aminomethane</Para>
</Description>
</DefinitionListEntry>
<DefinitionListEntry><Term>TCA</Term>
<Description><Para>trichloroacetic acid</Para>
</Description>
</DefinitionListEntry>
</DefinitionList>
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<abstract lang="en">Abstract: Polyribosomes (polysomes), active in an amino acid incorporation system in vitro, were isolated from tobacco leaf protoplasts. A comparison of polysome profiles indicated that the polysome/monosome ratio is greatly decreased in isolated protoplasts as compared to the intact leaf. In isolated protoplasts, a marked accumulation of ribosomal subunits was also found. The division of protoplasts, as investigated in the 8-cell and callus stages, was associated with a(n) (at least) partial regeneration of polysome profiles characteristic for leaves. Plasmolysis of leaves attached to the plant had no great effect on the polysome profile. However, leaf excision per se resulted in a dramatic loss of polysomes, even when the leaf tissue was floated on water. It is concluded that the isolation of the cell from its normal environment, and not the osmotic stress and associated increase in RNase activity, is the most important factor responsible for the loss of polysomes in isolated protoplasts.</abstract>
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<topic>Nicotiana</topic>
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<topic>Stress</topic>
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<topic>EGTA : ethylene glycol bis (2-aminoethyl ether)-tetraacetic acid</topic>
<topic>HEPES : N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid</topic>
<topic>mRNA : messenger ribonucleic acid</topic>
<topic>RNase : ribonuclease</topic>
<topic>Tris : tris(hydroxymethyl)aminomethane</topic>
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