Polyribosomes in protoplasts isolated from tobacco leaves
Identifieur interne : 000101 ( Istex/Corpus ); précédent : 000100; suivant : 000102Polyribosomes in protoplasts isolated from tobacco leaves
Auteurs : P. Ruzicska ; R. Mettrie ; Y. L. Dorokhov ; G. Premecz ; T. Oláh ; G. L. FarkasSource :
- Planta [ 0032-0935 ] ; 1979-01-01.
English descriptors
- KwdEn :
- Teeft :
- Callus, Extraction medium, Farkas, Larkins, Leaf tissue, Leaf tissues, Osmotic stress, Physiol, Plant physiol, Plant protoplasts, Polyribosomes, Polysome, Polysome classes, Polysome preparations, Polysome profiles, Polysomes, Premecz, Protein synthesis, Protoplast, Ribosomal subunits, Rnase, Sucrose density gradient profiles, Tobacco leaf protoplasts, Tobacco protoplasts.
Abstract
Abstract: Polyribosomes (polysomes), active in an amino acid incorporation system in vitro, were isolated from tobacco leaf protoplasts. A comparison of polysome profiles indicated that the polysome/monosome ratio is greatly decreased in isolated protoplasts as compared to the intact leaf. In isolated protoplasts, a marked accumulation of ribosomal subunits was also found. The division of protoplasts, as investigated in the 8-cell and callus stages, was associated with a(n) (at least) partial regeneration of polysome profiles characteristic for leaves. Plasmolysis of leaves attached to the plant had no great effect on the polysome profile. However, leaf excision per se resulted in a dramatic loss of polysomes, even when the leaf tissue was floated on water. It is concluded that the isolation of the cell from its normal environment, and not the osmotic stress and associated increase in RNase activity, is the most important factor responsible for the loss of polysomes in isolated protoplasts.
Url:
DOI: 10.1007/BF00388718
Links to Exploration step
ISTEX:623043FE171838BB5C87856E84711244AFF848E9Le document en format XML
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Dorokhov</name><affiliation><mods:affiliation>Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary</mods:affiliation></affiliation></author><author><name sortKey="Premecz, G" sort="Premecz, G" uniqKey="Premecz G" first="G." last="Premecz">G. Premecz</name><affiliation><mods:affiliation>Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary</mods:affiliation></affiliation></author><author><name sortKey="Olah, T" sort="Olah, T" uniqKey="Olah T" first="T." last="Oláh">T. Oláh</name><affiliation><mods:affiliation>Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary</mods:affiliation></affiliation></author><author><name sortKey="Farkas, G L" sort="Farkas, G L" uniqKey="Farkas G" first="G. L." last="Farkas">G. L. 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Ruzicska</name><affiliation><mods:affiliation>Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary</mods:affiliation></affiliation></author><author><name sortKey="Mettrie, R" sort="Mettrie, R" uniqKey="Mettrie R" first="R." last="Mettrie">R. Mettrie</name><affiliation><mods:affiliation>Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary</mods:affiliation></affiliation></author><author><name sortKey="Dorokhov, Y L" sort="Dorokhov, Y L" uniqKey="Dorokhov Y" first="Y. L." last="Dorokhov">Y. L. Dorokhov</name><affiliation><mods:affiliation>Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary</mods:affiliation></affiliation></author><author><name sortKey="Premecz, G" sort="Premecz, G" uniqKey="Premecz G" first="G." last="Premecz">G. Premecz</name><affiliation><mods:affiliation>Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary</mods:affiliation></affiliation></author><author><name sortKey="Olah, T" sort="Olah, T" uniqKey="Olah T" first="T." last="Oláh">T. Oláh</name><affiliation><mods:affiliation>Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary</mods:affiliation></affiliation></author><author><name sortKey="Farkas, G L" sort="Farkas, G L" uniqKey="Farkas G" first="G. L." last="Farkas">G. L. Farkas</name><affiliation><mods:affiliation>Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Planta</title><title level="j" type="sub">An International Journal of Plant Biology</title><title level="j" type="abbrev">Planta</title><idno type="ISSN">0032-0935</idno><idno type="eISSN">1432-2048</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1979-01-01">1979-01-01</date><biblScope unit="volume">145</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="199">199</biblScope><biblScope unit="page" to="203">203</biblScope></imprint><idno type="ISSN">0032-0935</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0032-0935</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Nicotiana</term><term>Polysomes</term><term>Protein synthesis</term><term>Protoplasts</term><term>Ribonuclease</term><term>Ribosomes</term><term>Stress</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Callus</term><term>Extraction medium</term><term>Farkas</term><term>Larkins</term><term>Leaf tissue</term><term>Leaf tissues</term><term>Osmotic stress</term><term>Physiol</term><term>Plant physiol</term><term>Plant protoplasts</term><term>Polyribosomes</term><term>Polysome</term><term>Polysome classes</term><term>Polysome preparations</term><term>Polysome profiles</term><term>Polysomes</term><term>Premecz</term><term>Protein synthesis</term><term>Protoplast</term><term>Ribosomal subunits</term><term>Rnase</term><term>Sucrose density gradient profiles</term><term>Tobacco leaf protoplasts</term><term>Tobacco protoplasts</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Polyribosomes (polysomes), active in an amino acid incorporation system in vitro, were isolated from tobacco leaf protoplasts. A comparison of polysome profiles indicated that the polysome/monosome ratio is greatly decreased in isolated protoplasts as compared to the intact leaf. In isolated protoplasts, a marked accumulation of ribosomal subunits was also found. The division of protoplasts, as investigated in the 8-cell and callus stages, was associated with a(n) (at least) partial regeneration of polysome profiles characteristic for leaves. Plasmolysis of leaves attached to the plant had no great effect on the polysome profile. However, leaf excision per se resulted in a dramatic loss of polysomes, even when the leaf tissue was floated on water. It is concluded that the isolation of the cell from its normal environment, and not the osmotic stress and associated increase in RNase activity, is the most important factor responsible for the loss of polysomes in isolated protoplasts.</div></front></TEI><istex><corpusName>springer-journals</corpusName><keywords><teeft><json:string>protoplast</json:string><json:string>polysome</json:string><json:string>polysomes</json:string><json:string>polyribosomes</json:string><json:string>rnase</json:string><json:string>physiol</json:string><json:string>premecz</json:string><json:string>plant physiol</json:string><json:string>polysome profiles</json:string><json:string>callus</json:string><json:string>osmotic stress</json:string><json:string>farkas</json:string><json:string>protein synthesis</json:string><json:string>tobacco leaf protoplasts</json:string><json:string>polysome preparations</json:string><json:string>larkins</json:string><json:string>plant protoplasts</json:string><json:string>extraction medium</json:string><json:string>sucrose density gradient profiles</json:string><json:string>polysome classes</json:string><json:string>leaf tissues</json:string><json:string>leaf tissue</json:string><json:string>ribosomal subunits</json:string><json:string>tobacco protoplasts</json:string></teeft></keywords><author><json:item><name>P. Ruzicska</name><affiliations><json:string>Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary</json:string></affiliations></json:item><json:item><name>R. Mettrie</name><affiliations><json:string>Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary</json:string></affiliations></json:item><json:item><name>Y. L. Dorokhov</name><affiliations><json:string>Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary</json:string></affiliations></json:item><json:item><name>G. Premecz</name><affiliations><json:string>Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary</json:string></affiliations></json:item><json:item><name>T. Oláh</name><affiliations><json:string>Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary</json:string></affiliations></json:item><json:item><name>G. L. Farkas</name><affiliations><json:string>Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Nicotiana</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Polysomes</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Protein synthesis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Protoplasts</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Ribonuclease</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Ribosomes</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Stress</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>EGTA : ethylene glycol bis (2-aminoethyl ether)-tetraacetic acid</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>HEPES : N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>mRNA : messenger ribonucleic acid</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>RNase : ribonuclease</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Tris : tris(hydroxymethyl)aminomethane</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>TCA : trichloroacetic acid</value></json:item></subject><articleId><json:string>BF00388718</json:string><json:string>Art13</json:string></articleId><arkIstex>ark:/67375/1BB-FDX4JMVK-K</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Abstract: Polyribosomes (polysomes), active in an amino acid incorporation system in vitro, were isolated from tobacco leaf protoplasts. A comparison of polysome profiles indicated that the polysome/monosome ratio is greatly decreased in isolated protoplasts as compared to the intact leaf. In isolated protoplasts, a marked accumulation of ribosomal subunits was also found. The division of protoplasts, as investigated in the 8-cell and callus stages, was associated with a(n) (at least) partial regeneration of polysome profiles characteristic for leaves. Plasmolysis of leaves attached to the plant had no great effect on the polysome profile. However, leaf excision per se resulted in a dramatic loss of polysomes, even when the leaf tissue was floated on water. It is concluded that the isolation of the cell from its normal environment, and not the osmotic stress and associated increase in RNase activity, is the most important factor responsible for the loss of polysomes in isolated protoplasts.</abstract><qualityIndicators><score>7.197</score><pdfWordCount>3373</pdfWordCount><pdfCharCount>18594</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>5</pdfPageCount><pdfPageSize>594 x 792 pts</pdfPageSize><refBibsNative>false</refBibsNative><abstractWordCount>152</abstractWordCount><abstractCharCount>1005</abstractCharCount><keywordCount>13</keywordCount></qualityIndicators><title>Polyribosomes in protoplasts isolated from tobacco leaves</title><pmid><json:string>24317677</json:string></pmid><genre><json:string>research-article</json:string></genre><host><title>Planta</title><language><json:string>unknown</json:string></language><publicationDate>1979</publicationDate><copyrightDate>1979</copyrightDate><issn><json:string>0032-0935</json:string></issn><eissn><json:string>1432-2048</json:string></eissn><journalId><json:string>425</json:string></journalId><volume>145</volume><issue>2</issue><pages><first>199</first><last>203</last></pages><genre><json:string>journal</json:string></genre><subject><json:item><value>Agriculture</value></json:item><json:item><value>Ecology</value></json:item><json:item><value>Forestry</value></json:item><json:item><value>Plant Sciences</value></json:item></subject></host><namedEntities><unitex><date></date><geogName></geogName><orgName></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName></persName><placeName></placeName><ref_url></ref_url><ref_bibl></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/1BB-FDX4JMVK-K</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - plant sciences</json:string></wos><scienceMetrix><json:string>1 - natural sciences</json:string><json:string>2 - biology</json:string><json:string>3 - plant biology & botany</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Agricultural and Biological Sciences</json:string><json:string>3 - Plant Science</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Genetics</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string><json:string>4 - psychologie. psychophysiologie</json:string></inist></categories><publicationDate>1979</publicationDate><copyrightDate>1979</copyrightDate><doi><json:string>10.1007/BF00388718</json:string></doi><id>623043FE171838BB5C87856E84711244AFF848E9</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/1BB-FDX4JMVK-K/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/1BB-FDX4JMVK-K/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/1BB-FDX4JMVK-K/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Polyribosomes in protoplasts isolated from tobacco leaves</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><availability><licence><p>Springer-Verlag, 1979</p></licence><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</p></availability><date>1978-11-09</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Polyribosomes in protoplasts isolated from tobacco leaves</title><author xml:id="author-0000"><persName><forename type="first">P.</forename><surname>Ruzicska</surname></persName><affiliation>Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary</affiliation></author><author xml:id="author-0001"><persName><forename type="first">R.</forename><surname>Mettrie</surname></persName><note type="biography">UNDP Research Fellow</note><affiliation>UNDP Research Fellow</affiliation><affiliation>Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Y.</forename><surname>Dorokhov</surname></persName><affiliation>Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary</affiliation></author><author xml:id="author-0003"><persName><forename type="first">G.</forename><surname>Premecz</surname></persName><affiliation>Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary</affiliation></author><author xml:id="author-0004"><persName><forename type="first">T.</forename><surname>Oláh</surname></persName><affiliation>Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary</affiliation></author><author xml:id="author-0005"><persName><forename type="first">G.</forename><surname>Farkas</surname></persName><affiliation>Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary</affiliation></author><idno type="istex">623043FE171838BB5C87856E84711244AFF848E9</idno><idno type="ark">ark:/67375/1BB-FDX4JMVK-K</idno><idno type="DOI">10.1007/BF00388718</idno><idno type="article-id">BF00388718</idno><idno type="article-id">Art13</idno></analytic><monogr><title level="j">Planta</title><title level="j" type="sub">An International Journal of Plant Biology</title><title level="j" type="abbrev">Planta</title><idno type="pISSN">0032-0935</idno><idno type="eISSN">1432-2048</idno><idno type="journal-ID">true</idno><idno type="issue-article-count">15</idno><idno type="volume-issue-count">5</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1979-01-01"></date><biblScope unit="volume">145</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="199">199</biblScope><biblScope unit="page" to="203">203</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1978-11-09</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Polyribosomes (polysomes), active in an amino acid incorporation system in vitro, were isolated from tobacco leaf protoplasts. A comparison of polysome profiles indicated that the polysome/monosome ratio is greatly decreased in isolated protoplasts as compared to the intact leaf. In isolated protoplasts, a marked accumulation of ribosomal subunits was also found. The division of protoplasts, as investigated in the 8-cell and callus stages, was associated with a(n) (at least) partial regeneration of polysome profiles characteristic for leaves. Plasmolysis of leaves attached to the plant had no great effect on the polysome profile. However, leaf excision per se resulted in a dramatic loss of polysomes, even when the leaf tissue was floated on water. It is concluded that the isolation of the cell from its normal environment, and not the osmotic stress and associated increase in RNase activity, is the most important factor responsible for the loss of polysomes in isolated protoplasts.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Key words</head><item><term>Nicotiana</term></item><item><term>Polysomes</term></item><item><term>Protein synthesis</term></item><item><term>Protoplasts</term></item><item><term>Ribonuclease</term></item><item><term>Ribosomes</term></item><item><term>Stress</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list><head>Abbreviations</head><item><term>EGTA</term><term>ethylene glycol bis (2-aminoethyl ether)-tetraacetic acid</term></item><item><term>HEPES</term><term>N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid</term></item><item><term>mRNA</term><term>messenger ribonucleic acid</term></item><item><term>RNase</term><term>ribonuclease</term></item><item><term>Tris</term><term>tris(hydroxymethyl)aminomethane</term></item><item><term>TCA</term><term>trichloroacetic acid</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>Life Sciences</head><item><term>Agriculture</term></item><item><term>Ecology</term></item><item><term>Forestry</term></item><item><term>Plant Sciences</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1978-11-09">Created</change><change when="1979-01-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/1BB-FDX4JMVK-K/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus springer-journals not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" name="istex:docType"></istex:docType><istex:document><Publisher><PublisherInfo><PublisherName>Springer-Verlag</PublisherName><PublisherLocation>Berlin/Heidelberg</PublisherLocation></PublisherInfo><Journal><JournalInfo JournalProductType="ArchiveJournal" NumberingStyle="Unnumbered"><JournalID>425</JournalID><JournalPrintISSN>0032-0935</JournalPrintISSN><JournalElectronicISSN>1432-2048</JournalElectronicISSN><JournalTitle>Planta</JournalTitle><JournalSubTitle>An International Journal of Plant Biology</JournalSubTitle><JournalAbbreviatedTitle>Planta</JournalAbbreviatedTitle><JournalSubjectGroup><JournalSubject Type="Primary">Life Sciences</JournalSubject><JournalSubject Type="Secondary">Agriculture</JournalSubject><JournalSubject Type="Secondary">Ecology</JournalSubject><JournalSubject Type="Secondary">Forestry</JournalSubject><JournalSubject Type="Secondary">Plant Sciences</JournalSubject></JournalSubjectGroup></JournalInfo><Volume><VolumeInfo VolumeType="Regular" TocLevels="0"><VolumeIDStart>145</VolumeIDStart><VolumeIDEnd>145</VolumeIDEnd><VolumeIssueCount>5</VolumeIssueCount></VolumeInfo><Issue IssueType="Regular"><IssueInfo TocLevels="0"><IssueIDStart>2</IssueIDStart><IssueIDEnd>2</IssueIDEnd><IssueArticleCount>15</IssueArticleCount><IssueHistory><CoverDate><DateString>22. III. 1979</DateString><Year>1979</Year><Month>1</Month></CoverDate></IssueHistory><IssueCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>1979</CopyrightYear></IssueCopyright></IssueInfo><Article ID="Art13"><ArticleInfo Language="En" ArticleType="OriginalPaper" NumberingStyle="Unnumbered" TocLevels="0" ContainsESM="No"><ArticleID>BF00388718</ArticleID><ArticleDOI>10.1007/BF00388718</ArticleDOI><ArticleSequenceNumber>13</ArticleSequenceNumber><ArticleTitle Language="En">Polyribosomes in protoplasts isolated from tobacco leaves</ArticleTitle><ArticleFirstPage>199</ArticleFirstPage><ArticleLastPage>203</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2004</Year><Month>9</Month><Day>14</Day></RegistrationDate><Received><Year>1978</Year><Month>11</Month><Day>9</Day></Received><Accepted><Year>1978</Year><Month>12</Month><Day>10</Day></Accepted></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>1979</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants><ArticleContext><JournalID>425</JournalID><VolumeIDStart>145</VolumeIDStart><VolumeIDEnd>145</VolumeIDEnd><IssueIDStart>2</IssueIDStart><IssueIDEnd>2</IssueIDEnd></ArticleContext></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>P.</GivenName><FamilyName>Ruzicska</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1" PresentAffiliationID="Aff2"><AuthorName DisplayOrder="Western"><GivenName>R.</GivenName><FamilyName>Mettrie</FamilyName></AuthorName><Role>UNDP Research Fellow</Role></Author><Author AffiliationIDS="Aff1" PresentAffiliationID="Aff3"><AuthorName DisplayOrder="Western"><GivenName>Y.</GivenName><GivenName>L.</GivenName><FamilyName>Dorokhov</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>G.</GivenName><FamilyName>Premecz</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>T.</GivenName><FamilyName>Oláh</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>G.</GivenName><GivenName>L.</GivenName><FamilyName>Farkas</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgDivision>Institute of Plant Physiology, Biological Research Center</OrgDivision><OrgName>Hungarian Academy of Sciences</OrgName><OrgAddress><Postbox>P.O. Box 521</Postbox><Postcode>H-6701</Postcode><City>Szeged</City><Country>Hungary</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>Department of Cell Biology</OrgDivision><OrgName>University of Antwerp</OrgName><OrgAddress><Street>Universiteitsplain 1</Street><Postcode>B-2610</Postcode><City>Wilrijk</City><Country>Belgium</Country></OrgAddress></Affiliation><Affiliation ID="Aff3"><OrgDivision>A.N. Belozersky Laboratory of Bioorganic Chemistry and Molecular Biology</OrgDivision><OrgName>Moscow State University</OrgName><OrgAddress><City>Moscow</City><Country>USSR</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading><Para>Polyribosomes (polysomes), active in an amino acid incorporation system in vitro, were isolated from tobacco leaf protoplasts. A comparison of polysome profiles indicated that the polysome/monosome ratio is greatly decreased in isolated protoplasts as compared to the intact leaf. In isolated protoplasts, a marked accumulation of ribosomal subunits was also found. The division of protoplasts, as investigated in the 8-cell and callus stages, was associated with a(n) (at least) partial regeneration of polysome profiles characteristic for leaves. Plasmolysis of leaves attached to the plant had no great effect on the polysome profile. However, leaf excision per se resulted in a dramatic loss of polysomes, even when the leaf tissue was floated on water. It is concluded that the isolation of the cell from its normal environment, and not the osmotic stress and associated increase in RNase activity, is the most important factor responsible for the loss of polysomes in isolated protoplasts.</Para></Abstract><KeywordGroup Language="En"><Heading>Key words</Heading><Keyword><Emphasis Type="Italic">Nicotiana</Emphasis></Keyword><Keyword>Polysomes</Keyword><Keyword>Protein synthesis</Keyword><Keyword>Protoplasts</Keyword><Keyword>Ribonuclease</Keyword><Keyword>Ribosomes</Keyword><Keyword>Stress</Keyword></KeywordGroup><AbbreviationGroup><Heading>Abbreviations</Heading><DefinitionList><DefinitionListEntry><Term>EGTA</Term><Description><Para>ethylene glycol bis (2-aminoethyl ether)-tetraacetic acid</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>HEPES</Term><Description><Para>N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>mRNA</Term><Description><Para>messenger ribonucleic acid</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>RNase</Term><Description><Para>ribonuclease</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>Tris</Term><Description><Para>tris(hydroxymethyl)aminomethane</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>TCA</Term><Description><Para>trichloroacetic acid</Para></Description></DefinitionListEntry></DefinitionList></AbbreviationGroup></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Polyribosomes in protoplasts isolated from tobacco leaves</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Polyribosomes in protoplasts isolated from tobacco leaves</title></titleInfo><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Ruzicska</namePart><affiliation>Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R.</namePart><namePart type="family">Mettrie</namePart><affiliation>Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary</affiliation><role><roleTerm type="text">author</roleTerm></role><description>UNDP Research Fellow</description></name><name type="personal"><namePart type="given">Y.</namePart><namePart type="given">L.</namePart><namePart type="family">Dorokhov</namePart><affiliation>Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="family">Premecz</namePart><affiliation>Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">T.</namePart><namePart type="family">Oláh</namePart><affiliation>Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="given">L.</namePart><namePart type="family">Farkas</namePart><affiliation>Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer-Verlag</publisher><place><placeTerm type="text">Berlin/Heidelberg</placeTerm></place><dateCreated encoding="w3cdtf">1978-11-09</dateCreated><dateIssued encoding="w3cdtf">1979-01-01</dateIssued><copyrightDate encoding="w3cdtf">1979</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: Polyribosomes (polysomes), active in an amino acid incorporation system in vitro, were isolated from tobacco leaf protoplasts. A comparison of polysome profiles indicated that the polysome/monosome ratio is greatly decreased in isolated protoplasts as compared to the intact leaf. In isolated protoplasts, a marked accumulation of ribosomal subunits was also found. The division of protoplasts, as investigated in the 8-cell and callus stages, was associated with a(n) (at least) partial regeneration of polysome profiles characteristic for leaves. Plasmolysis of leaves attached to the plant had no great effect on the polysome profile. However, leaf excision per se resulted in a dramatic loss of polysomes, even when the leaf tissue was floated on water. It is concluded that the isolation of the cell from its normal environment, and not the osmotic stress and associated increase in RNase activity, is the most important factor responsible for the loss of polysomes in isolated protoplasts.</abstract><subject lang="en"><genre>Key words</genre><topic>Nicotiana</topic><topic>Polysomes</topic><topic>Protein synthesis</topic><topic>Protoplasts</topic><topic>Ribonuclease</topic><topic>Ribosomes</topic><topic>Stress</topic></subject><subject><genre>Abbreviations</genre><topic>EGTA : ethylene glycol bis (2-aminoethyl ether)-tetraacetic acid</topic><topic>HEPES : N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid</topic><topic>mRNA : messenger ribonucleic acid</topic><topic>RNase : ribonuclease</topic><topic>Tris : tris(hydroxymethyl)aminomethane</topic><topic>TCA : trichloroacetic acid</topic></subject><relatedItem type="host"><titleInfo><title>Planta</title><subTitle>An International Journal of Plant Biology</subTitle></titleInfo><titleInfo type="abbreviated"><title>Planta</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">1979-01-01</dateIssued><copyrightDate encoding="w3cdtf">1979</copyrightDate></originInfo><subject><genre>Life Sciences</genre><topic>Agriculture</topic><topic>Ecology</topic><topic>Forestry</topic><topic>Plant Sciences</topic></subject><identifier type="ISSN">0032-0935</identifier><identifier type="eISSN">1432-2048</identifier><identifier type="JournalID">425</identifier><identifier type="IssueArticleCount">15</identifier><identifier type="VolumeIssueCount">5</identifier><part><date>1979</date><detail type="volume"><number>145</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="pages"><start>199</start><end>203</end></extent></part><recordInfo><recordOrigin>Springer-Verlag, 1979</recordOrigin></recordInfo></relatedItem><identifier type="istex">623043FE171838BB5C87856E84711244AFF848E9</identifier><identifier type="ark">ark:/67375/1BB-FDX4JMVK-K</identifier><identifier type="DOI">10.1007/BF00388718</identifier><identifier type="ArticleID">BF00388718</identifier><identifier type="ArticleID">Art13</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer-Verlag, 1979</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Springer-Verlag, 1979</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/1BB-FDX4JMVK-K/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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