MersV1, Istex, Corpus, bibRecord, 000099

Synthetic polyamines stimulate in vitro transcription by T7 RNA polymerase

Identifieur interne : 000099 ( Istex/Corpus ); précédent : 000098; suivant : 000100

Synthetic polyamines stimulate in vitro transcription by T7 RNA polymerase

Auteurs : Magali Frugier ; Catherine Florentz ; Mir Wais Hosseini ; Jean-Marie Lehn ; Richard Giegé

Source :

Nucleic Acids Research [ 0305-1048 ] ; 1994.

RBID : ISTEX:3257ECF5F1C7E888BB790311B53D71B9AAD8EF97

Abstract

The influence of nine synthetic polyamines on in vitro transcription with T7 RNA polymerase has been studied. The compounds used were linear or macrocyclic tetra- and hexaamine, varying in their size, shape and number of protonated groups. Their effect was tested on different types of templates, all presenting the T7 RNA promoter in a double-stranded form followed by sequences encoding short transcripts 25 to 35-mers) either on single- or double-stranded synthetic oligodeoxyribonucleotides. All polyamines used stimulate transcription of both types of templates at levels dependent on their size, shape, protonation degree, and concentration. For each compound, an optimal concentration could be defined; above this concentration, transcription inhibition occurred. Highest stimulation (up to 12-fold) was obtained by the largest cyclic compound called [38JN6C10.

Url:

https://api.istex.fr/ark:/67375/HXZ-2SJ12GLQ-0/fulltext.pdf

DOI: 10.1093/nar/22.14.2784

Links to Exploration step

ISTEX:3257ECF5F1C7E888BB790311B53D71B9AAD8EF97

Le document en format XML

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<issn pub-type="epub">1362-4962</issn>

<issn pub-type="ppub">0305-1048</issn>

<publisher>

<publisher-name>Oxford University Press</publisher-name>

</publisher>

</journal-meta>

<article-meta>

<article-id pub-id-type="publisher-id">22.14.2784</article-id>

<article-id pub-id-type="doi">10.1093/nar/22.14.2784</article-id>

<article-categories>

<subj-group>

<subject>MOLECULAR BIOLOGY</subject>

</subj-group>

</article-categories>

<title-group>

<article-title>Synthetic polyamines stimulate <italic>in vitro</italic>
 transcription by T7 RNA polymerase</article-title>

</title-group>

<contrib-group>

<contrib contrib-type="author">

<name><surname>Frugier</surname>
<given-names>Magali</given-names>
</name>

</contrib>

<contrib contrib-type="author">

<name><surname>Florentz</surname>
<given-names>Catherine</given-names>
</name>

</contrib>

<contrib contrib-type="author">

<name><surname>Hosseini</surname>
<given-names>Mir Wais</given-names>
</name>
<xref ref-type="aff" rid="au1"><sup>1</sup>
</xref>

</contrib>

<contrib contrib-type="author">

<name><surname>Lehn</surname>
<given-names>Jean-Marie</given-names>
</name>
<xref ref-type="aff" rid="au2"><sup>2</sup>
</xref>

</contrib>

<contrib contrib-type="author">

<name><surname>Giegé</surname>
<given-names>Richard</given-names>
</name>
<xref ref-type="corresp" rid="cor1"><sup>*</sup>
</xref>

</contrib>

<aff><institution>UPR 'Structure des Macromolécules Biologiques et Mécanismes de Reconnaissance' Institut de Biologie Moléculaire et Cellulaire du CNRS</institution>
 <addr-line>15 rue René Descartes, F-67084 Strasbourg Cedex</addr-line>
</aff>

<aff id="au1"><sup>1</sup>
<institution>Laboratoire de Chimie de Coordination Organique, Institut Le Bel</institution>
 <addr-line>4 rue Blaise Pascal, F-67000 Strasbourg Cedex, France</addr-line>
</aff>

<aff id="au2"><sup>2</sup>
<institution>Laboratoire de Chimie Supramoléculaire</institution>
 <addr-line>Institut Le Bel, 4 rue Blaise Pascal, F-67000 Strasbourg Cedex, France</addr-line>
</aff>

</contrib-group>

<author-notes>

<corresp id="cor1"><sup>*</sup>
To whom correspondence should be addressed</corresp>

</author-notes>

<pub-date pub-type="ppub">

<day>25</day>

<month>7</month>

<year>1994</year>

</pub-date>

<volume>22</volume>

<issue>14</issue>

<fpage>2784</fpage>

<lpage>2790</lpage>

<history>

<date date-type="received">

<day>13</day>

<month>4</month>

<year>1994</year>

</date>

<date date-type="rev-recd">

<day>22</day>

<month>6</month>

<year>1994</year>

</date>

<date date-type="accepted">

<day>22</day>

<month>6</month>

<year>1994</year>

</date>

</history>

<copyright-statement>© 1994 Oxford University Press</copyright-statement>

<copyright-year>1994</copyright-year>

<abstract>

<p>The influence of nine synthetic polyamines on <italic>in vitro</italic>
 transcription with T7 RNA polymerase has been studied. The compounds used were linear or macrocyclic tetra- and hexaamine, varying in their size, shape and number of protonated groups. Their effect was tested on different types of templates, all presenting the T7 RNA promoter in a double-stranded form followed by sequences encoding short transcripts 25 to 35-mers) either on single- or double-stranded synthetic oligodeoxyribonucleotides. All polyamines used stimulate transcription of both types of templates at levels dependent on their size, shape, protonation degree, and concentration. For each compound, an optimal concentration could be defined; above this concentration, transcription inhibition occurred. Highest stimulation (up to 12-fold) was obtained by the largest cyclic compound called [38JN6C10.</p>

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Synthetic polyamines stimulate in vitro transcription by T7 RNA polymerase

Synthetic polyamines stimulate in vitro transcription by T7 RNA polymerase

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