Three Dimensional Reconstructions of Spherical Viruses by Fourier Synthesis from Electron Micrographs
Identifieur interne : 002633 ( Istex/Checkpoint ); précédent : 002632; suivant : 002634Three Dimensional Reconstructions of Spherical Viruses by Fourier Synthesis from Electron Micrographs
Auteurs : R. A. Crowther [États-Unis] ; Linda A. Amos [États-Unis] ; J. T. Finch [États-Unis] ; D. J. De Rosier ; A. Klug [États-Unis]Source :
- Nature [ 0028-0836 ] ; 1970-05-02.
Abstract
Methods are developed for computing three dimensional density maps from transmission electron micrographs using Fourier transforms. The reconstructions objectively combine data from several different views of one or more particles. When applied to negatively stained spherical viruses, the technique gives clear reconstructions of their surface structures.
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DOI: 10.1038/226421a0
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Crowther</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Texas</region></placeName><wicri:cityArea>MRC Laboratory of Molecular Biology, Hills Road, CambridgePresent address: Department of Chemistry, University of Texas, Austin</wicri:cityArea></affiliation></author><author><name sortKey="Amos, Linda A" sort="Amos, Linda A" uniqKey="Amos L" first="Linda A." last="Amos">Linda A. Amos</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Texas</region></placeName><wicri:cityArea>MRC Laboratory of Molecular Biology, Hills Road, CambridgePresent address: Department of Chemistry, University of Texas, Austin</wicri:cityArea></affiliation></author><author><name sortKey="Finch, J T" sort="Finch, J T" uniqKey="Finch J" first="J. T." last="Finch">J. T. Finch</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Texas</region></placeName><wicri:cityArea>MRC Laboratory of Molecular Biology, Hills Road, CambridgePresent address: Department of Chemistry, University of Texas, Austin</wicri:cityArea></affiliation></author><author><name sortKey="De Rosier, D J" sort="De Rosier, D J" uniqKey="De Rosier D" first="D. J." last="De Rosier">D. J. De Rosier</name></author><author><name sortKey="Klug, A" sort="Klug, A" uniqKey="Klug A" first="A." last="Klug">A. Klug</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Texas</region></placeName><wicri:cityArea>MRC Laboratory of Molecular Biology, Hills Road, CambridgePresent address: Department of Chemistry, University of Texas, Austin</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Nature</title><imprint><publisher>Nature Publishing Group</publisher><date when="1970-05-02">1970-05-02</date><biblScope unit="vol">226</biblScope><biblScope unit="issue">5244</biblScope><biblScope unit="page" from="421">421</biblScope><biblScope unit="page" to="425">425</biblScope><date type="Copyright" when="1970">1970</date></imprint><idno type="ISSN">0028-0836</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0028-0836</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">Methods are developed for computing three dimensional density maps from transmission electron micrographs using Fourier transforms. The reconstructions objectively combine data from several different views of one or more particles. When applied to negatively stained spherical viruses, the technique gives clear reconstructions of their surface structures.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Texas</li></region></list><tree><noCountry><name sortKey="De Rosier, D J" sort="De Rosier, D J" uniqKey="De Rosier D" first="D. J." last="De Rosier">D. J. De Rosier</name></noCountry><country name="États-Unis"><region name="Texas"><name sortKey="Crowther, R A" sort="Crowther, R A" uniqKey="Crowther R" first="R. A." last="Crowther">R. A. Crowther</name></region><name sortKey="Amos, Linda A" sort="Amos, Linda A" uniqKey="Amos L" first="Linda A." last="Amos">Linda A. Amos</name><name sortKey="Finch, J T" sort="Finch, J T" uniqKey="Finch J" first="J. T." last="Finch">J. T. Finch</name><name sortKey="Klug, A" sort="Klug, A" uniqKey="Klug A" first="A." last="Klug">A. 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