Recombination in bacteriophage P2: recA dependent enhancement by ultraviolet irradiation and by transfection with mixed DNA dimers
Identifieur interne : 002430 ( Istex/Checkpoint ); précédent : 002429; suivant : 002431Recombination in bacteriophage P2: recA dependent enhancement by ultraviolet irradiation and by transfection with mixed DNA dimers
Auteurs : T. Hudnik-Plevnik [Suède] ; G. Bertani [Suède]Source :
- Molecular and General Genetics MGG [ 0026-8925 ] ; 1980-04-01.
English descriptors
- Teeft :
- Acad, Agar, Assay, Bacteriophage, Bertani, Biol, Burst size, Circular dimers, Circular monomers, Cjcd, Cjld, Clone, Cohesive ends, Coli, Confluent spot, Covalently, Daughter plaques, Derivative, Dimer, Escherichia, Escherichia coli, General recombination, Genetic recombination, Genotype, High frequency, Host bacteria, Host bacterium, Host recombination pathway, Indicator bacteria, Infective, Infective centers, Lambda, Latent period, Ligase, Ligated dimers, Lindahl, Linear dimers, Linear monomers, Main recombination pathway, Monomer, Monomer mixtures, Mutant, Mutation, Nac1, Natl, Neutral sucrose gradient, Parent phages, Parental, Parental types, Pathway, Phage, Phage particles, Phage progeny, Plaque, Plaque content analysis, Plating, Proc, Progeny, Progeny phage, Reca, Recombinant, Recombinant phage, Recombinant type, Recombination, Recombination experiments, Recombination frequency, Replication, Selective conditions, Soft agar, Spheroplasts, Spontaneous recombination, Streptomycin, Sucrose, Superinfecting phage, Transfected, Transfected spheroplasts, Transfection, Transfection assay, Transfections, Tsd4, Ultraviolet irradiation, Ultraviolet light, Upper part, Virology, Yielder.
Abstract
Summary: Bacteriophage P2 is known for its exceptionally low rate of spontaneous (non-integrative) recombination, which however may be stimulated by ultraviolet irradiation of the phage. We show here that ligated dimers, made in vitro from mixtures of DNAs of two P2 mutants, upon transfection of lysozyme-spheroplasts give origin to recombinants at high frequency. While spontaneous P2 recombination occurs independently of the main recombination pathway of the bacteria, P2 recombinant formation following either ultraviolet irradiation or transfection with DNA dimers requires at least some element of such a pathway, since it is absent or greatly reduced in recA - bacteria or spheroplasts. It would seem that, in the course of its lytic development, P2 deploys a mechanism that inhibits the main recombination pathway of the host cell, or assumes DNA configurations refractory to it.
Url:
DOI: 10.1007/BF00267221
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
ISTEX:0F6B9436EA92F48508347554B67E1E61759100E2Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Recombination in bacteriophage P2: recA dependent enhancement by ultraviolet irradiation and by transfection with mixed DNA dimers</title><author><name sortKey="Hudnik Plevnik, T" sort="Hudnik Plevnik, T" uniqKey="Hudnik Plevnik T" first="T." last="Hudnik-Plevnik">T. Hudnik-Plevnik</name></author><author><name sortKey="Bertani, G" sort="Bertani, G" uniqKey="Bertani G" first="G." last="Bertani">G. Bertani</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:0F6B9436EA92F48508347554B67E1E61759100E2</idno><date when="1980" year="1980">1980</date><idno type="doi">10.1007/BF00267221</idno><idno type="url">https://api.istex.fr/ark:/67375/1BB-XX71FT63-J/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">002273</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002273</idno><idno type="wicri:Area/Istex/Curation">002273</idno><idno type="wicri:Area/Istex/Checkpoint">002430</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">002430</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Recombination in bacteriophage P2: recA dependent enhancement by ultraviolet irradiation and by transfection with mixed DNA dimers</title><author><name sortKey="Hudnik Plevnik, T" sort="Hudnik Plevnik, T" uniqKey="Hudnik Plevnik T" first="T." last="Hudnik-Plevnik">T. Hudnik-Plevnik</name><affiliation wicri:level="3"><country xml:lang="fr">Suède</country><wicri:regionArea>Microbial Genetics Laboratory, Karolinska Institutet, S-10401, Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName></affiliation></author><author><name sortKey="Bertani, G" sort="Bertani, G" uniqKey="Bertani G" first="G." last="Bertani">G. Bertani</name><affiliation wicri:level="3"><country xml:lang="fr">Suède</country><wicri:regionArea>Microbial Genetics Laboratory, Karolinska Institutet, S-10401, Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Molecular and General Genetics MGG</title><title level="j" type="abbrev">Molec. Gen. Genet.</title><idno type="ISSN">0026-8925</idno><idno type="eISSN">1432-1874</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1980-04-01">1980-04-01</date><biblScope unit="volume">178</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="131">131</biblScope><biblScope unit="page" to="141">141</biblScope></imprint><idno type="ISSN">0026-8925</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0026-8925</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acad</term><term>Agar</term><term>Assay</term><term>Bacteriophage</term><term>Bertani</term><term>Biol</term><term>Burst size</term><term>Circular dimers</term><term>Circular monomers</term><term>Cjcd</term><term>Cjld</term><term>Clone</term><term>Cohesive ends</term><term>Coli</term><term>Confluent spot</term><term>Covalently</term><term>Daughter plaques</term><term>Derivative</term><term>Dimer</term><term>Escherichia</term><term>Escherichia coli</term><term>General recombination</term><term>Genetic recombination</term><term>Genotype</term><term>High frequency</term><term>Host bacteria</term><term>Host bacterium</term><term>Host recombination pathway</term><term>Indicator bacteria</term><term>Infective</term><term>Infective centers</term><term>Lambda</term><term>Latent period</term><term>Ligase</term><term>Ligated dimers</term><term>Lindahl</term><term>Linear dimers</term><term>Linear monomers</term><term>Main recombination pathway</term><term>Monomer</term><term>Monomer mixtures</term><term>Mutant</term><term>Mutation</term><term>Nac1</term><term>Natl</term><term>Neutral sucrose gradient</term><term>Parent phages</term><term>Parental</term><term>Parental types</term><term>Pathway</term><term>Phage</term><term>Phage particles</term><term>Phage progeny</term><term>Plaque</term><term>Plaque content analysis</term><term>Plating</term><term>Proc</term><term>Progeny</term><term>Progeny phage</term><term>Reca</term><term>Recombinant</term><term>Recombinant phage</term><term>Recombinant type</term><term>Recombination</term><term>Recombination experiments</term><term>Recombination frequency</term><term>Replication</term><term>Selective conditions</term><term>Soft agar</term><term>Spheroplasts</term><term>Spontaneous recombination</term><term>Streptomycin</term><term>Sucrose</term><term>Superinfecting phage</term><term>Transfected</term><term>Transfected spheroplasts</term><term>Transfection</term><term>Transfection assay</term><term>Transfections</term><term>Tsd4</term><term>Ultraviolet irradiation</term><term>Ultraviolet light</term><term>Upper part</term><term>Virology</term><term>Yielder</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: Bacteriophage P2 is known for its exceptionally low rate of spontaneous (non-integrative) recombination, which however may be stimulated by ultraviolet irradiation of the phage. We show here that ligated dimers, made in vitro from mixtures of DNAs of two P2 mutants, upon transfection of lysozyme-spheroplasts give origin to recombinants at high frequency. While spontaneous P2 recombination occurs independently of the main recombination pathway of the bacteria, P2 recombinant formation following either ultraviolet irradiation or transfection with DNA dimers requires at least some element of such a pathway, since it is absent or greatly reduced in recA - bacteria or spheroplasts. It would seem that, in the course of its lytic development, P2 deploys a mechanism that inhibits the main recombination pathway of the host cell, or assumes DNA configurations refractory to it.</div></front></TEI><affiliations><list><country><li>Suède</li></country><region><li>Svealand</li></region><settlement><li>Stockholm</li></settlement></list><tree><country name="Suède"><region name="Svealand"><name sortKey="Hudnik Plevnik, T" sort="Hudnik Plevnik, T" uniqKey="Hudnik Plevnik T" first="T." last="Hudnik-Plevnik">T. Hudnik-Plevnik</name></region><name sortKey="Bertani, G" sort="Bertani, G" uniqKey="Bertani G" first="G." last="Bertani">G. Bertani</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002430 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Checkpoint/biblio.hfd -nk 002430 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Istex
|étape= Checkpoint
|type= RBID
|clé= ISTEX:0F6B9436EA92F48508347554B67E1E61759100E2
|texte= Recombination in bacteriophage P2: recA dependent enhancement by ultraviolet irradiation and by transfection with mixed DNA dimers
}}
| This area was generated with Dilib version V0.6.33. |  |