Molecular cloning and analysis of a cDNA coding for the bifunctional dihydrofolate reductase-thymidylate synthase of Daucus carota
Identifieur interne : 001C49 ( Istex/Checkpoint ); précédent : 001C48; suivant : 001C50Molecular cloning and analysis of a cDNA coding for the bifunctional dihydrofolate reductase-thymidylate synthase of Daucus carota
Auteurs : Meizhong Luo [Italie] ; Pietro Piffanelli [Italie] ; Luca Rastelli [Italie] ; Rino Cella [Italie]Source :
- Plant Molecular Biology [ 0167-4412 ] ; 1993-06-01.
English descriptors
- KwdEn :
- Teeft :
- Acad, Amino, Amino acid sequence, Amino acid sequences, Arch biochem biophys, Bifunctional, Bifunctional dihydrofolate synthase, Bifunctional protein, Bifunctional thymidylate reductase, Biol, Biol chem, Carota, Carrot, Carrot cells, Carrot protein, Cdna coding, Celia, Daucus, Daucus carota, Dihydrofolate, Dihydrofolate reductase, Dihydrofolate synthase, Escherichia coli, Folate, Gene content, Gene encoding, High level, Higher degree, Homoserine dehydrogenase activities, Leishmania tropica, Limited proteolysis, Monofunctional, Natl, Nucleotide sequence, Open reading frame, Other sources, Phage, Photoaffinity labelling, Plant physiol, Polypeptide, Polypeptide regions, Primary structure, Proc, Proc natl acad, Random hexanucleotides, Recombinant phages, Reconstructed experiment, Reductase, Saccharomyces cerevisiae, Sequence analysis, Several copies, Several reasons, Solid line, Soybean seedlings, Specific activity, Synthase, Thymidylate, Thymidylate synthase, Tropica, Vertebrate.
Abstract
Abstract: Molecular cloning of dihydrofolate reductase-thymidylate synthase (DHFR-TS) of Daucus carota was achieved by immunoscreening of a cDNA library obtaining a 2 kbp clone which contains an open reading frame of 1528 bp. Comparison of the deduced amino acid sequence with those from other sources revealed the presence of motifs typical of DHFR and TS thus confirming the bifunctional nature of the carrot protein. As in other organisms, a higher degree of conservation was observed in the TS domain. Analysis of the dhfr-ts gene content in carrot revealed the presence of several copies per diploid genome.
Url:
DOI: 10.1007/BF00015973
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
ISTEX:17560010A72691E930A0CAD4976B533F8B2F418DLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular cloning and analysis of a cDNA coding for the bifunctional dihydrofolate reductase-thymidylate synthase of Daucus carota</title>
<author><name sortKey="Luo, Meizhong" sort="Luo, Meizhong" uniqKey="Luo M" first="Meizhong" last="Luo">Meizhong Luo</name>
</author>
<author><name sortKey="Piffanelli, Pietro" sort="Piffanelli, Pietro" uniqKey="Piffanelli P" first="Pietro" last="Piffanelli">Pietro Piffanelli</name>
</author>
<author><name sortKey="Rastelli, Luca" sort="Rastelli, Luca" uniqKey="Rastelli L" first="Luca" last="Rastelli">Luca Rastelli</name>
</author>
<author><name sortKey="Cella, Rino" sort="Cella, Rino" uniqKey="Cella R" first="Rino" last="Cella">Rino Cella</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:17560010A72691E930A0CAD4976B533F8B2F418D</idno>
<date when="1993" year="1993">1993</date>
<idno type="doi">10.1007/BF00015973</idno>
<idno type="url">https://api.istex.fr/ark:/67375/1BB-THB643SG-3/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001107</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001107</idno>
<idno type="wicri:Area/Istex/Curation">001107</idno>
<idno type="wicri:Area/Istex/Checkpoint">001C49</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001C49</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Molecular cloning and analysis of a cDNA coding for the bifunctional dihydrofolate reductase-thymidylate synthase of Daucus carota</title>
<author><name sortKey="Luo, Meizhong" sort="Luo, Meizhong" uniqKey="Luo M" first="Meizhong" last="Luo">Meizhong Luo</name>
<affiliation wicri:level="1"><country xml:lang="fr">Italie</country>
<wicri:regionArea>Dipartimento di Genetica e Microbiologia, Sezione di Microbiologia e Fisiologia Vegetale, Università di Pavia, via Abbiategrasso 207, 27100, Pavia</wicri:regionArea>
<wicri:noRegion>Pavia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Piffanelli, Pietro" sort="Piffanelli, Pietro" uniqKey="Piffanelli P" first="Pietro" last="Piffanelli">Pietro Piffanelli</name>
<affiliation wicri:level="1"><country xml:lang="fr">Italie</country>
<wicri:regionArea>Dipartimento di Genetica e Microbiologia, Sezione di Microbiologia e Fisiologia Vegetale, Università di Pavia, via Abbiategrasso 207, 27100, Pavia</wicri:regionArea>
<wicri:noRegion>Pavia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Rastelli, Luca" sort="Rastelli, Luca" uniqKey="Rastelli L" first="Luca" last="Rastelli">Luca Rastelli</name>
<affiliation wicri:level="1"><country xml:lang="fr">Italie</country>
<wicri:regionArea>Dipartimento di Genetica e Microbiologia, Sezione di Microbiologia e Fisiologia Vegetale, Università di Pavia, via Abbiategrasso 207, 27100, Pavia</wicri:regionArea>
<wicri:noRegion>Pavia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cella, Rino" sort="Cella, Rino" uniqKey="Cella R" first="Rino" last="Cella">Rino Cella</name>
<affiliation wicri:level="1"><country xml:lang="fr">Italie</country>
<wicri:regionArea>Dipartimento di Genetica e Microbiologia, Sezione di Microbiologia e Fisiologia Vegetale, Università di Pavia, via Abbiategrasso 207, 27100, Pavia</wicri:regionArea>
<wicri:noRegion>Pavia</wicri:noRegion>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Plant Molecular Biology</title>
<title level="j" type="sub">An International Journal on Molecular Biology, Molecular Genetics and Biochemistry</title>
<title level="j" type="abbrev">Plant Mol Biol</title>
<idno type="ISSN">0167-4412</idno>
<idno type="eISSN">1573-5028</idno>
<imprint><publisher>Kluwer Academic Publishers</publisher>
<pubPlace>Dordrecht</pubPlace>
<date type="published" when="1993-06-01">1993-06-01</date>
<biblScope unit="volume">22</biblScope>
<biblScope unit="issue">3</biblScope>
<biblScope unit="page" from="427">427</biblScope>
<biblScope unit="page" to="435">435</biblScope>
</imprint>
<idno type="ISSN">0167-4412</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0167-4412</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Daucus carota</term>
<term>amino acid sequence</term>
<term>bifunctional protein</term>
<term>cDNA cloning</term>
<term>dihydrofolate</term>
<term>immunoscreening</term>
<term>reductase-thymidylate synthase</term>
</keywords>
<keywords scheme="Teeft" xml:lang="en"><term>Acad</term>
<term>Amino</term>
<term>Amino acid sequence</term>
<term>Amino acid sequences</term>
<term>Arch biochem biophys</term>
<term>Bifunctional</term>
<term>Bifunctional dihydrofolate synthase</term>
<term>Bifunctional protein</term>
<term>Bifunctional thymidylate reductase</term>
<term>Biol</term>
<term>Biol chem</term>
<term>Carota</term>
<term>Carrot</term>
<term>Carrot cells</term>
<term>Carrot protein</term>
<term>Cdna coding</term>
<term>Celia</term>
<term>Daucus</term>
<term>Daucus carota</term>
<term>Dihydrofolate</term>
<term>Dihydrofolate reductase</term>
<term>Dihydrofolate synthase</term>
<term>Escherichia coli</term>
<term>Folate</term>
<term>Gene content</term>
<term>Gene encoding</term>
<term>High level</term>
<term>Higher degree</term>
<term>Homoserine dehydrogenase activities</term>
<term>Leishmania tropica</term>
<term>Limited proteolysis</term>
<term>Monofunctional</term>
<term>Natl</term>
<term>Nucleotide sequence</term>
<term>Open reading frame</term>
<term>Other sources</term>
<term>Phage</term>
<term>Photoaffinity labelling</term>
<term>Plant physiol</term>
<term>Polypeptide</term>
<term>Polypeptide regions</term>
<term>Primary structure</term>
<term>Proc</term>
<term>Proc natl acad</term>
<term>Random hexanucleotides</term>
<term>Recombinant phages</term>
<term>Reconstructed experiment</term>
<term>Reductase</term>
<term>Saccharomyces cerevisiae</term>
<term>Sequence analysis</term>
<term>Several copies</term>
<term>Several reasons</term>
<term>Solid line</term>
<term>Soybean seedlings</term>
<term>Specific activity</term>
<term>Synthase</term>
<term>Thymidylate</term>
<term>Thymidylate synthase</term>
<term>Tropica</term>
<term>Vertebrate</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: Molecular cloning of dihydrofolate reductase-thymidylate synthase (DHFR-TS) of Daucus carota was achieved by immunoscreening of a cDNA library obtaining a 2 kbp clone which contains an open reading frame of 1528 bp. Comparison of the deduced amino acid sequence with those from other sources revealed the presence of motifs typical of DHFR and TS thus confirming the bifunctional nature of the carrot protein. As in other organisms, a higher degree of conservation was observed in the TS domain. Analysis of the dhfr-ts gene content in carrot revealed the presence of several copies per diploid genome.</div>
</front>
</TEI>
<affiliations><list><country><li>Italie</li>
</country>
</list>
<tree><country name="Italie"><noRegion><name sortKey="Luo, Meizhong" sort="Luo, Meizhong" uniqKey="Luo M" first="Meizhong" last="Luo">Meizhong Luo</name>
</noRegion>
<name sortKey="Cella, Rino" sort="Cella, Rino" uniqKey="Cella R" first="Rino" last="Cella">Rino Cella</name>
<name sortKey="Piffanelli, Pietro" sort="Piffanelli, Pietro" uniqKey="Piffanelli P" first="Pietro" last="Piffanelli">Pietro Piffanelli</name>
<name sortKey="Rastelli, Luca" sort="Rastelli, Luca" uniqKey="Rastelli L" first="Luca" last="Rastelli">Luca Rastelli</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001C49 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Checkpoint/biblio.hfd -nk 001C49 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Istex |étape= Checkpoint |type= RBID |clé= ISTEX:17560010A72691E930A0CAD4976B533F8B2F418D |texte= Molecular cloning and analysis of a cDNA coding for the bifunctional dihydrofolate reductase-thymidylate synthase of Daucus carota }}
![]() | This area was generated with Dilib version V0.6.33. | ![]() |