Molecular cloning and analysis of a cDNA coding for the bifunctional dihydrofolate reductase-thymidylate synthase of Daucus carota
Identifieur interne : 001C49 ( Istex/Checkpoint ); précédent : 001C48; suivant : 001C50Molecular cloning and analysis of a cDNA coding for the bifunctional dihydrofolate reductase-thymidylate synthase of Daucus carota
Auteurs : Meizhong Luo [Italie] ; Pietro Piffanelli [Italie] ; Luca Rastelli [Italie] ; Rino Cella [Italie]Source :
- Plant Molecular Biology [ 0167-4412 ] ; 1993-06-01.
English descriptors
- KwdEn :
- Teeft :
- Acad, Amino, Amino acid sequence, Amino acid sequences, Arch biochem biophys, Bifunctional, Bifunctional dihydrofolate synthase, Bifunctional protein, Bifunctional thymidylate reductase, Biol, Biol chem, Carota, Carrot, Carrot cells, Carrot protein, Cdna coding, Celia, Daucus, Daucus carota, Dihydrofolate, Dihydrofolate reductase, Dihydrofolate synthase, Escherichia coli, Folate, Gene content, Gene encoding, High level, Higher degree, Homoserine dehydrogenase activities, Leishmania tropica, Limited proteolysis, Monofunctional, Natl, Nucleotide sequence, Open reading frame, Other sources, Phage, Photoaffinity labelling, Plant physiol, Polypeptide, Polypeptide regions, Primary structure, Proc, Proc natl acad, Random hexanucleotides, Recombinant phages, Reconstructed experiment, Reductase, Saccharomyces cerevisiae, Sequence analysis, Several copies, Several reasons, Solid line, Soybean seedlings, Specific activity, Synthase, Thymidylate, Thymidylate synthase, Tropica, Vertebrate.
Abstract
Abstract: Molecular cloning of dihydrofolate reductase-thymidylate synthase (DHFR-TS) of Daucus carota was achieved by immunoscreening of a cDNA library obtaining a 2 kbp clone which contains an open reading frame of 1528 bp. Comparison of the deduced amino acid sequence with those from other sources revealed the presence of motifs typical of DHFR and TS thus confirming the bifunctional nature of the carrot protein. As in other organisms, a higher degree of conservation was observed in the TS domain. Analysis of the dhfr-ts gene content in carrot revealed the presence of several copies per diploid genome.
Url:
DOI: 10.1007/BF00015973
Affiliations:
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unit="volume">22</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="427">427</biblScope><biblScope unit="page" to="435">435</biblScope></imprint><idno type="ISSN">0167-4412</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0167-4412</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Daucus carota</term><term>amino acid sequence</term><term>bifunctional protein</term><term>cDNA cloning</term><term>dihydrofolate</term><term>immunoscreening</term><term>reductase-thymidylate synthase</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Acad</term><term>Amino</term><term>Amino acid sequence</term><term>Amino acid sequences</term><term>Arch biochem biophys</term><term>Bifunctional</term><term>Bifunctional dihydrofolate synthase</term><term>Bifunctional protein</term><term>Bifunctional thymidylate reductase</term><term>Biol</term><term>Biol chem</term><term>Carota</term><term>Carrot</term><term>Carrot cells</term><term>Carrot protein</term><term>Cdna coding</term><term>Celia</term><term>Daucus</term><term>Daucus carota</term><term>Dihydrofolate</term><term>Dihydrofolate reductase</term><term>Dihydrofolate synthase</term><term>Escherichia coli</term><term>Folate</term><term>Gene content</term><term>Gene encoding</term><term>High level</term><term>Higher degree</term><term>Homoserine dehydrogenase activities</term><term>Leishmania tropica</term><term>Limited proteolysis</term><term>Monofunctional</term><term>Natl</term><term>Nucleotide sequence</term><term>Open reading frame</term><term>Other sources</term><term>Phage</term><term>Photoaffinity labelling</term><term>Plant physiol</term><term>Polypeptide</term><term>Polypeptide regions</term><term>Primary structure</term><term>Proc</term><term>Proc natl acad</term><term>Random hexanucleotides</term><term>Recombinant phages</term><term>Reconstructed experiment</term><term>Reductase</term><term>Saccharomyces cerevisiae</term><term>Sequence analysis</term><term>Several copies</term><term>Several reasons</term><term>Solid line</term><term>Soybean seedlings</term><term>Specific activity</term><term>Synthase</term><term>Thymidylate</term><term>Thymidylate synthase</term><term>Tropica</term><term>Vertebrate</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Molecular cloning of dihydrofolate reductase-thymidylate synthase (DHFR-TS) of Daucus carota was achieved by immunoscreening of a cDNA library obtaining a 2 kbp clone which contains an open reading frame of 1528 bp. Comparison of the deduced amino acid sequence with those from other sources revealed the presence of motifs typical of DHFR and TS thus confirming the bifunctional nature of the carrot protein. As in other organisms, a higher degree of conservation was observed in the TS domain. Analysis of the dhfr-ts gene content in carrot revealed the presence of several copies per diploid genome.</div></front></TEI><affiliations><list><country><li>Italie</li></country></list><tree><country name="Italie"><noRegion><name sortKey="Luo, Meizhong" sort="Luo, Meizhong" uniqKey="Luo M" first="Meizhong" last="Luo">Meizhong Luo</name></noRegion><name sortKey="Cella, Rino" sort="Cella, Rino" uniqKey="Cella R" first="Rino" last="Cella">Rino Cella</name><name sortKey="Piffanelli, Pietro" sort="Piffanelli, Pietro" uniqKey="Piffanelli P" first="Pietro" last="Piffanelli">Pietro Piffanelli</name><name sortKey="Rastelli, Luca" sort="Rastelli, Luca" uniqKey="Rastelli L" first="Luca" last="Rastelli">Luca Rastelli</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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