Assay for Movement of Lepidopteran Transposon IFP2 in Insect Cells Using a Baculovirus Genome as a Target DNA
Identifieur interne : 001911 ( Istex/Checkpoint ); précédent : 001910; suivant : 001912Assay for Movement of Lepidopteran Transposon IFP2 in Insect Cells Using a Baculovirus Genome as a Target DNA
Auteurs : Malcolm J. Fraser [États-Unis] ; Lynne Cary [États-Unis] ; Kitima Boonvisudhi [États-Unis] ; Hwei-Gene Heidi Wang [États-Unis]Source :
- Virology [ 0042-6822 ] ; 1995.
Abstract
Abstract: Mutagenesis of baculoviruses by host mobile elements occurs spontaneously and frequently during propagation of the viruses in Lepidopteran cell cultures. Most of the transposons identified as insertions in baculovirus genomes are relatively small Class II elements that exhibit a remarkable specificity for TTAA target sites. We have developed a transposition assay to analyze the movement of these TTAA-specific Lepidopteran transposons using the baculovirus genome as a target and a lacZ gene under control of the polyhedrin gene promoter as a selective marker for the transposon. This assay provides the first demonstration that a Lepidopteran transposon is capable of transposing while carrying a marker gene in insect cells. The data generated from this assay provide strong evidence that IFP2 encodes a protein that facilitates its own movement. This element may be used in a manner analogous to the P-element to mobilize genes in at least some Lepidopteran insect cells. Transposon tagging within the baculovirus genome identified several known genes and two previously undescribed open reading frames as nonessential to in vitro replication of the virus.
Url:
DOI: 10.1006/viro.1995.1422
Affiliations:
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Fraser</name></author><author><name sortKey="Cary, Lynne" sort="Cary, Lynne" uniqKey="Cary L" first="Lynne" last="Cary">Lynne Cary</name></author><author><name sortKey="Boonvisudhi, Kitima" sort="Boonvisudhi, Kitima" uniqKey="Boonvisudhi K" first="Kitima" last="Boonvisudhi">Kitima Boonvisudhi</name></author><author><name sortKey="Wang, Hwei Gene Heidi" sort="Wang, Hwei Gene Heidi" uniqKey="Wang H" first="Hwei-Gene Heidi" last="Wang">Hwei-Gene Heidi Wang</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:076D0E2F7D3547E7AFAA02ABFE1D4912179031ED</idno><date when="1995" year="1995">1995</date><idno type="doi">10.1006/viro.1995.1422</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-9S760GLP-F/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">002044</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002044</idno><idno type="wicri:Area/Istex/Curation">002044</idno><idno type="wicri:Area/Istex/Checkpoint">001911</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001911</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Assay for Movement of Lepidopteran Transposon IFP2 in Insect Cells Using a Baculovirus Genome as a Target DNA</title><author><name sortKey="Fraser, Malcolm J" sort="Fraser, Malcolm J" uniqKey="Fraser M" first="Malcolm J." last="Fraser">Malcolm J. Fraser</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Indiana</region></placeName><wicri:cityArea>Department of Biological Sciences, The University of Notre Dame, Notre Dame</wicri:cityArea></affiliation></author><author><name sortKey="Cary, Lynne" sort="Cary, Lynne" uniqKey="Cary L" first="Lynne" last="Cary">Lynne Cary</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Indiana</region></placeName><wicri:cityArea>Department of Biological Sciences, The University of Notre Dame, Notre Dame</wicri:cityArea></affiliation></author><author><name sortKey="Boonvisudhi, Kitima" sort="Boonvisudhi, Kitima" uniqKey="Boonvisudhi K" first="Kitima" last="Boonvisudhi">Kitima Boonvisudhi</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Indiana</region></placeName><wicri:cityArea>Department of Biological Sciences, The University of Notre Dame, Notre Dame</wicri:cityArea></affiliation></author><author><name sortKey="Wang, Hwei Gene Heidi" sort="Wang, Hwei Gene Heidi" uniqKey="Wang H" first="Hwei-Gene Heidi" last="Wang">Hwei-Gene Heidi Wang</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Indiana</region></placeName><wicri:cityArea>Department of Biological Sciences, The University of Notre Dame, Notre Dame</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1995">1995</date><biblScope unit="volume">211</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="397">397</biblScope><biblScope unit="page" to="407">407</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Mutagenesis of baculoviruses by host mobile elements occurs spontaneously and frequently during propagation of the viruses in Lepidopteran cell cultures. Most of the transposons identified as insertions in baculovirus genomes are relatively small Class II elements that exhibit a remarkable specificity for TTAA target sites. We have developed a transposition assay to analyze the movement of these TTAA-specific Lepidopteran transposons using the baculovirus genome as a target and a lacZ gene under control of the polyhedrin gene promoter as a selective marker for the transposon. This assay provides the first demonstration that a Lepidopteran transposon is capable of transposing while carrying a marker gene in insect cells. The data generated from this assay provide strong evidence that IFP2 encodes a protein that facilitates its own movement. This element may be used in a manner analogous to the P-element to mobilize genes in at least some Lepidopteran insect cells. Transposon tagging within the baculovirus genome identified several known genes and two previously undescribed open reading frames as nonessential to in vitro replication of the virus.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Indiana</li></region></list><tree><country name="États-Unis"><region name="Indiana"><name sortKey="Fraser, Malcolm J" sort="Fraser, Malcolm J" uniqKey="Fraser M" first="Malcolm J." last="Fraser">Malcolm J. Fraser</name></region><name sortKey="Boonvisudhi, Kitima" sort="Boonvisudhi, Kitima" uniqKey="Boonvisudhi K" first="Kitima" last="Boonvisudhi">Kitima Boonvisudhi</name><name sortKey="Cary, Lynne" sort="Cary, Lynne" uniqKey="Cary L" first="Lynne" last="Cary">Lynne Cary</name><name sortKey="Wang, Hwei Gene Heidi" sort="Wang, Hwei Gene Heidi" uniqKey="Wang H" first="Hwei-Gene Heidi" last="Wang">Hwei-Gene Heidi Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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