Characterization of structural features important for T7 RNAP elongation complex stability reveals competing complex conformations and a role for the non-template strand in RNA displacement

Characterization of structural features important for T7 RNAP elongation complex stability reveals competing complex conformations and a role for the non-template strand in RNA displacement

Source :

• Journal of Molecular Biology [ 0022-2836 ] ; 1999.

English descriptors

• KwdEn :
  • D.S., EC, EtBr, NT, P.S.S., RNA:DNA hybrid, RNAP, T7 RNA polymerase, ThΔ, elongation complex, processivity, transcription.
• Teeft :
  • Acad, Active elongation, Apparent rnase, Bacteriophage, Base transcripts, Binary complexes, Binding activity, Binding site, Biochemistry, Biol, Chem, Coli, Coli rnap, Conformation, Cutoff membranes, Ddtmp incorporation, Ddttp, Degradation products, Denaturation, Dissociation rates, Dnase, Duplex, Duplex templates, Elongation, Enzyme, Enzyme forms, Enzyme reaction, Escherichia, Escherichia coli, Hairpin formation, Hybrid, Hybrid formation, Ikeda, Ikeda richardson, Intact enzyme, Intact enzymes, Lilley houghton, Linear duplex templates, Linear template, Linear templates, Linearized, Ltrate, Mcallister, Misincorporation, Molar amount, Multisubunit rnaps, Mutant, Natl, Natl acad, Ntps, Persistent hybrids, Plasmid, Polymerase, Poor activity, Proc, Processivity, Promoter, Proteolytic nicking, Reannealing, Reannealing template, Replication, Replication origins, Retenate, Rna, Rnap, Rnap elongation, Rnaps, Rnase, Room temperature, Runoff, Runoff product, Runoff transcript, Runoff transcription, Runoff transcripts, Short transcripts, Sousa, Stability figure, Strand, Strand invasion, Strand reannealing, Subdomain, Such conditions, Such hybrids, Such templates, Supercoiled, Supercoiled template, Supercoiled templates, Supercoiling, Synthetic promoters, Template, Template reannealing, Template strand, Template structure, Terminator, Thumb subdomain, Time point, Transcript, Transcript generation, Transcript synthesis, Transcription, Transcription bubble, Transcription reactions, Turnover, Turnover rate, Turnover rates, Unreacted.

Abstract

• https://api.istex.fr/ark:/67375/6H6-88HHMTKB-B/fulltext.pdf

Affiliations:

• États-Unis
• État de New York

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