Growth control of mouse mammary epithelial cells by keratin antibody‐targeted liposome containing oligonucleotides antisense to epidermal growth factor receptor
Identifieur interne : 000C80 ( Istex/Checkpoint ); précédent : 000C79; suivant : 000C81Growth control of mouse mammary epithelial cells by keratin antibody‐targeted liposome containing oligonucleotides antisense to epidermal growth factor receptor
Auteurs : In-Suh Yuh [Corée du Sud] ; Seung-Youp Lee ; Byong-Ju HongSource :
- Cell Biology International [ 1065-6995 ] ; 2003-05.
Abstract
NMuMG cells were incubated with 17β‐estradiol (E)+progesterone (P)+epidermal growth factor (EGF), with or without various types of oligomers (21‐mers) to the EGF receptor activity domain (amino acid residues 718 to 724). Sense or antisense oligomers were encapsulated in protein A‐bearing liposomes. Uncoupled protein A and unencapsulated sense or antisense oligomers were separated from liposomes on a Sepharose 4B column (the encapsulation efficiency of oligomers in liposome—protein A was 0.8%). The addition of various concentrations of EGF to E+P showed an interaction between them during DNA synthesis (P<0.05). Antisense oligomers (1 μM) decreased DNA synthesis induced by E+P+EGF (65.0% inhibition, P<0.05). Sense oligomers also inhibited DNA synthesis induced by E+P+EGF (P<0.05). However, random‐sequence oligomers did not inhibit EGF‐induced DNA synthesis. We cannot rule out the possibility that sense oligomers match an unknown functional gene mRNA involved in cell growth, which causes their inhibitory effect. Cells were incubated with a keratin monoclonal antibody and then with dilutions of protein A‐bound liposomes containing sense or antisense oligomers in the presence of E+P+EGF. Dose dependent inhibition of DNA synthesis was observed. The encapsulated oligomers in protein A‐bound liposomes inhibited DNA synthesis at a 100‐fold lower concentration than that of unencapsulated oligomers or the oligomer+liposome mixture. The tyrosine kinase activity domain has an important role in EGF regulation of mammary growth. The effect of a cytokeratin‐targeted antibody on DNA synthesis in normal mouse mammary epithelial cells was marginal.
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DOI: 10.1016/S1065-6995(03)00020-9
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Growth control of mouse mammary epithelial cells by keratin antibody‐targeted liposome containing oligonucleotides antisense to epidermal growth factor receptor</title><author><name sortKey="Yuh, In Uh" sort="Yuh, In Uh" uniqKey="Yuh I" first="In-Suh" last="Yuh">In-Suh Yuh</name></author><author><name sortKey="Lee, Seung Oup" sort="Lee, Seung Oup" uniqKey="Lee S" first="Seung-Youp" last="Lee">Seung-Youp Lee</name></author><author><name sortKey="Hong, Byong U" sort="Hong, Byong U" uniqKey="Hong B" first="Byong-Ju" last="Hong">Byong-Ju Hong</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:E618B790C43A0DDB5A3E546DA0EE1866A38BEA10</idno><date when="2003" year="2003">2003</date><idno type="doi">10.1016/S1065-6995(03)00020-9</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-09QMW1V3-Z/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000592</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000592</idno><idno type="wicri:Area/Istex/Curation">000592</idno><idno type="wicri:Area/Istex/Checkpoint">000C80</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000C80</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Growth control of mouse mammary epithelial cells by keratin antibody‐targeted liposome containing oligonucleotides antisense to epidermal growth factor receptor</title><author><name sortKey="Yuh, In Uh" sort="Yuh, In Uh" uniqKey="Yuh I" first="In-Suh" last="Yuh">In-Suh Yuh</name><affiliation></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Corée du Sud</country></affiliation></author><author><name sortKey="Lee, Seung Oup" sort="Lee, Seung Oup" uniqKey="Lee S" first="Seung-Youp" last="Lee">Seung-Youp Lee</name><affiliation><wicri:noCountry code="subField">200‐701</wicri:noCountry></affiliation></author><author><name sortKey="Hong, Byong U" sort="Hong, Byong U" uniqKey="Hong B" first="Byong-Ju" last="Hong">Byong-Ju Hong</name><affiliation><wicri:noCountry code="subField">200‐701</wicri:noCountry></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Cell Biology International</title><title level="j" type="alt">CELL BIOLOGY INTERNATIONAL</title><idno type="ISSN">1065-6995</idno><idno type="eISSN">1095-8355</idno><imprint><biblScope unit="vol">27</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="409">409</biblScope><biblScope unit="page" to="414">414</biblScope><biblScope unit="page-count">6</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2003-05">2003-05</date></imprint><idno type="ISSN">1065-6995</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1065-6995</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">NMuMG cells were incubated with 17β‐estradiol (E)+progesterone (P)+epidermal growth factor (EGF), with or without various types of oligomers (21‐mers) to the EGF receptor activity domain (amino acid residues 718 to 724). Sense or antisense oligomers were encapsulated in protein A‐bearing liposomes. Uncoupled protein A and unencapsulated sense or antisense oligomers were separated from liposomes on a Sepharose 4B column (the encapsulation efficiency of oligomers in liposome—protein A was 0.8%). The addition of various concentrations of EGF to E+P showed an interaction between them during DNA synthesis (P<0.05). Antisense oligomers (1 μM) decreased DNA synthesis induced by E+P+EGF (65.0% inhibition, P<0.05). Sense oligomers also inhibited DNA synthesis induced by E+P+EGF (P<0.05). However, random‐sequence oligomers did not inhibit EGF‐induced DNA synthesis. We cannot rule out the possibility that sense oligomers match an unknown functional gene mRNA involved in cell growth, which causes their inhibitory effect. Cells were incubated with a keratin monoclonal antibody and then with dilutions of protein A‐bound liposomes containing sense or antisense oligomers in the presence of E+P+EGF. Dose dependent inhibition of DNA synthesis was observed. The encapsulated oligomers in protein A‐bound liposomes inhibited DNA synthesis at a 100‐fold lower concentration than that of unencapsulated oligomers or the oligomer+liposome mixture. The tyrosine kinase activity domain has an important role in EGF regulation of mammary growth. The effect of a cytokeratin‐targeted antibody on DNA synthesis in normal mouse mammary epithelial cells was marginal.</div></front></TEI><affiliations><list><country><li>Corée du Sud</li></country></list><tree><noCountry><name sortKey="Hong, Byong U" sort="Hong, Byong U" uniqKey="Hong B" first="Byong-Ju" last="Hong">Byong-Ju Hong</name><name sortKey="Lee, Seung Oup" sort="Lee, Seung Oup" uniqKey="Lee S" first="Seung-Youp" last="Lee">Seung-Youp Lee</name></noCountry><country name="Corée du Sud"><noRegion><name sortKey="Yuh, In Uh" sort="Yuh, In Uh" uniqKey="Yuh I" first="In-Suh" last="Yuh">In-Suh Yuh</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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