Assessment of synthetic peptides of severe acute respiratory syndrome coronavirus recognized by long‐lasting immunity
Identifieur interne : 000C35 ( Istex/Checkpoint ); précédent : 000C34; suivant : 000C36Assessment of synthetic peptides of severe acute respiratory syndrome coronavirus recognized by long‐lasting immunity
Auteurs : S. Shichijo [Japon] ; N. Keicho [Japon] ; H. T. Long [Viêt Nam] ; T. Quy [Viêt Nam] ; N. C. Phi [Viêt Nam] ; L. D. Ha [Viêt Nam] ; V. V. Ban [Viêt Nam] ; S. Itoyama [Japon] ; C. Hu [Taïwan] ; N. Komatsu [Japon] ; T. Kirikae [Japon] ; F. Kirikae [Japon] ; S. Shirasawa [Japon] ; M. Kaji [Japon] ; T. Fukuda [Japon] ; M. Sata [Japon] ; T. Kuratsuji [Japon] ; K. Itoh [Japon] ; T. Sasazuki [Japon]Source :
- Tissue Antigens [ 0001-2815 ] ; 2004-11.
English descriptors
- Teeft :
- Absorption test, Clin chem, Clinical manifestations, Contact persons, Coronavirus, Corresponding peptide, Dose dependency, Elisa, Epitope, Flowmetry analysis, Fluorescence intensity, Fluorescence intensity fluorescence intensity, Fluorescence intensity patient, Giai phong, Healthy donors, Higher levels, Humoral responses, Immunogenic, Immunogenic epitopes, Immunogenic regions, Japanese patients, Kinetic studies, Kurume, Kurume university school, Luminex, Luminex corp, Luminex reactivities, Negative controls, None peptides, Novel coronavirus, Nucleocapsid, Nucleocapsid protein, Peptide, Peptide peptide sequence, Plate shaker, Positions peptide, Protective vaccine, Reactive, Representative results, Respiratory syndrome, Respiratory syndrome coronavirus, Rheumatoid arthritis, Room temperature, Saan district, Same times, Sars, Sars patients, Sars peptides, Serum dilution, Serum samples, Shichijo, Significant levels, Syndrome, Synthetic peptides, Systemic lupus erythematosus, Taiwanese patients, Times dilution, Tissue antigens, Vaccine.
Abstract
Abstract: In order to determine highly immunogenic severe acute respiratory syndrome coronavirus (SARS‐CoV) epitope peptides capable of inducing long‐lasting immunity, we first screened immunoglobulin‐G (IgG) antibodies reactive to 197 different overlapping 15‐mers from the SARS‐CoV proteins in the sera of three infected patients. Forty‐two peptides among them were reactive to the sera from all three patients. Consequently, we tested for the reactivity of these 42 peptides to patients' sera (n = 45) at 6‐month post‐infection. The significantly higher levels of IgG antibodies specific to three (S791, M207 and N161) of 42 peptides were detectable in the post‐infection sera from 23 (51%), 27 (60%) and 19 (42%) of 45 patients, respectively. These three peptides, recognized by their long‐lasting immunity, may provide a better understanding of the immunogenicity of SARS‐CoV.
Url:
DOI: 10.1111/j.1399-0039.2004.00314.x
Affiliations:
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ISTEX:0144A752526A448C35F9BF8FE17D22DA1834C23DLe document en format XML
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<series><title level="j" type="main">Tissue Antigens</title>
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<term>Coronavirus</term>
<term>Corresponding peptide</term>
<term>Dose dependency</term>
<term>Elisa</term>
<term>Epitope</term>
<term>Flowmetry analysis</term>
<term>Fluorescence intensity</term>
<term>Fluorescence intensity fluorescence intensity</term>
<term>Fluorescence intensity patient</term>
<term>Giai phong</term>
<term>Healthy donors</term>
<term>Higher levels</term>
<term>Humoral responses</term>
<term>Immunogenic</term>
<term>Immunogenic epitopes</term>
<term>Immunogenic regions</term>
<term>Japanese patients</term>
<term>Kinetic studies</term>
<term>Kurume</term>
<term>Kurume university school</term>
<term>Luminex</term>
<term>Luminex corp</term>
<term>Luminex reactivities</term>
<term>Negative controls</term>
<term>None peptides</term>
<term>Novel coronavirus</term>
<term>Nucleocapsid</term>
<term>Nucleocapsid protein</term>
<term>Peptide</term>
<term>Peptide peptide sequence</term>
<term>Plate shaker</term>
<term>Positions peptide</term>
<term>Protective vaccine</term>
<term>Reactive</term>
<term>Representative results</term>
<term>Respiratory syndrome</term>
<term>Respiratory syndrome coronavirus</term>
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<term>Room temperature</term>
<term>Saan district</term>
<term>Same times</term>
<term>Sars</term>
<term>Sars patients</term>
<term>Sars peptides</term>
<term>Serum dilution</term>
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<term>Shichijo</term>
<term>Significant levels</term>
<term>Syndrome</term>
<term>Synthetic peptides</term>
<term>Systemic lupus erythematosus</term>
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<front><div type="abstract" xml:lang="en">Abstract: In order to determine highly immunogenic severe acute respiratory syndrome coronavirus (SARS‐CoV) epitope peptides capable of inducing long‐lasting immunity, we first screened immunoglobulin‐G (IgG) antibodies reactive to 197 different overlapping 15‐mers from the SARS‐CoV proteins in the sera of three infected patients. Forty‐two peptides among them were reactive to the sera from all three patients. Consequently, we tested for the reactivity of these 42 peptides to patients' sera (n = 45) at 6‐month post‐infection. The significantly higher levels of IgG antibodies specific to three (S791, M207 and N161) of 42 peptides were detectable in the post‐infection sera from 23 (51%), 27 (60%) and 19 (42%) of 45 patients, respectively. These three peptides, recognized by their long‐lasting immunity, may provide a better understanding of the immunogenicity of SARS‐CoV.</div>
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