Genome analyses suggest the presence of polyploidy and recent human‐driven expansions in eight global populations of the honeybee pathogen Nosema ceranae
Identifieur interne : 000131 ( Istex/Checkpoint ); précédent : 000130; suivant : 000132Genome analyses suggest the presence of polyploidy and recent human‐driven expansions in eight global populations of the honeybee pathogen Nosema ceranae
Auteurs : Adrian Pelin [Canada] ; Mohammed Selman [Canada] ; Stéphane Aris-Brosou [Canada] ; Laurent Farinelli [Suisse] ; Nicolas Corradi [Canada]Source :
- Environmental Microbiology [ 1462-2912 ] ; 2015-11.
Abstract
Nosema ceranae is a microsporidian pathogen whose infections have been associated with recent global declines in the populations of western honeybees (Apis mellifera). Despite the outstanding economic and ecological threat that N. ceranae may represent for honeybees worldwide, many aspects of its biology, including its mode of reproduction, propagation and ploidy, are either very unclear or unknown. In the present study, we set to gain knowledge in these biological aspects by re‐sequencing the genome of eight isolates (i.e. a population of spores isolated from one single beehive) of this species harvested from eight geographically distant beehives, and by investigating their level of polymorphism. Consistent with previous analyses performed using single gene sequences, our analyses uncovered the presence of very high genetic diversity within each isolate, but also very little hive‐specific polymorphism. Surprisingly, the nature, location and distribution of this genetic variation suggest that beehives around the globe are infected by a population of N. ceranae cells that may be polyploid (4n or more), and possibly clonal. Lastly, phylogenetic analyses based on genome‐wide single‐nucleotide polymorphism data extracted from these parasites and mitochondrial sequences from their hosts all failed to support the current geographical structure of our isolates.
Url:
DOI: 10.1111/1462-2920.12883
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
ISTEX:FB225EC1B1A04F3D1F6E0D16830345C2AC4F1FBDLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Genome analyses suggest the presence of polyploidy and recent human‐driven expansions in eight global populations of the honeybee pathogen Nosema ceranae</title><author><name sortKey="Pelin, Adrian" sort="Pelin, Adrian" uniqKey="Pelin A" first="Adrian" last="Pelin">Adrian Pelin</name></author><author><name sortKey="Selman, Mohammed" sort="Selman, Mohammed" uniqKey="Selman M" first="Mohammed" last="Selman">Mohammed Selman</name></author><author><name sortKey="Aris Rosou, Stephane" sort="Aris Rosou, Stephane" uniqKey="Aris Rosou S" first="Stéphane" last="Aris-Brosou">Stéphane Aris-Brosou</name></author><author><name sortKey="Farinelli, Laurent" sort="Farinelli, Laurent" uniqKey="Farinelli L" first="Laurent" last="Farinelli">Laurent Farinelli</name></author><author><name sortKey="Corradi, Nicolas" sort="Corradi, Nicolas" uniqKey="Corradi N" first="Nicolas" last="Corradi">Nicolas Corradi</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:FB225EC1B1A04F3D1F6E0D16830345C2AC4F1FBD</idno><date when="2015" year="2015">2015</date><idno type="doi">10.1111/1462-2920.12883</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-WNGTX902-T/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000116</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000116</idno><idno type="wicri:Area/Istex/Curation">000116</idno><idno type="wicri:Area/Istex/Checkpoint">000131</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000131</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Genome analyses suggest the presence of polyploidy and recent human‐driven expansions in eight global populations of the honeybee pathogen Nosema ceranae</title><author><name sortKey="Pelin, Adrian" sort="Pelin, Adrian" uniqKey="Pelin A" first="Adrian" last="Pelin">Adrian Pelin</name><affiliation wicri:level="1"><country xml:lang="fr">Canada</country><wicri:regionArea>Canadian Institute for Advanced Research, Department of Biology; University of Ottawa, ON, Ottawa</wicri:regionArea><wicri:noRegion>Ottawa</wicri:noRegion></affiliation></author><author><name sortKey="Selman, Mohammed" sort="Selman, Mohammed" uniqKey="Selman M" first="Mohammed" last="Selman">Mohammed Selman</name><affiliation wicri:level="1"><country xml:lang="fr">Canada</country><wicri:regionArea>Canadian Institute for Advanced Research, Department of Biology; University of Ottawa, ON, Ottawa</wicri:regionArea><wicri:noRegion>Ottawa</wicri:noRegion></affiliation></author><author><name sortKey="Aris Rosou, Stephane" sort="Aris Rosou, Stephane" uniqKey="Aris Rosou S" first="Stéphane" last="Aris-Brosou">Stéphane Aris-Brosou</name><affiliation wicri:level="1"><country xml:lang="fr">Canada</country><wicri:regionArea>Departments of Biology and of Mathematics & Statistics, University of Ottawa, ON, Ottawa</wicri:regionArea><wicri:noRegion>Ottawa</wicri:noRegion></affiliation></author><author><name sortKey="Farinelli, Laurent" sort="Farinelli, Laurent" uniqKey="Farinelli L" first="Laurent" last="Farinelli">Laurent Farinelli</name><affiliation wicri:level="1"><country xml:lang="fr">Suisse</country><wicri:regionArea>FASTERIS S.A., Ch. du Pont‐du‐Centenaire 109, P.O. Box 28, Plan‐les‐OuatesGeneva, CH‐1228</wicri:regionArea><wicri:noRegion>CH‐1228</wicri:noRegion></affiliation></author><author><name sortKey="Corradi, Nicolas" sort="Corradi, Nicolas" uniqKey="Corradi N" first="Nicolas" last="Corradi">Nicolas Corradi</name><affiliation wicri:level="1"><country xml:lang="fr">Canada</country><wicri:regionArea>Canadian Institute for Advanced Research, Department of Biology; University of Ottawa, ON, Ottawa</wicri:regionArea><wicri:noRegion>Ottawa</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Canada</country></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Environmental Microbiology</title><title level="j" type="sub">Pathogen Ecology</title><title level="j" type="alt">ENVIRONMENTAL MICROBIOLOGY</title><idno type="ISSN">1462-2912</idno><idno type="eISSN">1462-2920</idno><imprint><biblScope unit="vol">17</biblScope><biblScope unit="issue">11</biblScope><biblScope unit="page" from="4443">4443</biblScope><biblScope unit="page" to="4458">4458</biblScope><biblScope unit="page-count">16</biblScope><date type="published" when="2015-11">2015-11</date></imprint><idno type="ISSN">1462-2912</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1462-2912</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">Nosema ceranae is a microsporidian pathogen whose infections have been associated with recent global declines in the populations of western honeybees (Apis mellifera). Despite the outstanding economic and ecological threat that N. ceranae may represent for honeybees worldwide, many aspects of its biology, including its mode of reproduction, propagation and ploidy, are either very unclear or unknown. In the present study, we set to gain knowledge in these biological aspects by re‐sequencing the genome of eight isolates (i.e. a population of spores isolated from one single beehive) of this species harvested from eight geographically distant beehives, and by investigating their level of polymorphism. Consistent with previous analyses performed using single gene sequences, our analyses uncovered the presence of very high genetic diversity within each isolate, but also very little hive‐specific polymorphism. Surprisingly, the nature, location and distribution of this genetic variation suggest that beehives around the globe are infected by a population of N. ceranae cells that may be polyploid (4n or more), and possibly clonal. Lastly, phylogenetic analyses based on genome‐wide single‐nucleotide polymorphism data extracted from these parasites and mitochondrial sequences from their hosts all failed to support the current geographical structure of our isolates.</div></front></TEI><affiliations><list><country><li>Canada</li><li>Suisse</li></country></list><tree><country name="Canada"><noRegion><name sortKey="Pelin, Adrian" sort="Pelin, Adrian" uniqKey="Pelin A" first="Adrian" last="Pelin">Adrian Pelin</name></noRegion><name sortKey="Aris Rosou, Stephane" sort="Aris Rosou, Stephane" uniqKey="Aris Rosou S" first="Stéphane" last="Aris-Brosou">Stéphane Aris-Brosou</name><name sortKey="Corradi, Nicolas" sort="Corradi, Nicolas" uniqKey="Corradi N" first="Nicolas" last="Corradi">Nicolas Corradi</name><name sortKey="Corradi, Nicolas" sort="Corradi, Nicolas" uniqKey="Corradi N" first="Nicolas" last="Corradi">Nicolas Corradi</name><name sortKey="Selman, Mohammed" sort="Selman, Mohammed" uniqKey="Selman M" first="Mohammed" last="Selman">Mohammed Selman</name></country><country name="Suisse"><noRegion><name sortKey="Farinelli, Laurent" sort="Farinelli, Laurent" uniqKey="Farinelli L" first="Laurent" last="Farinelli">Laurent Farinelli</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000131 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Checkpoint/biblio.hfd -nk 000131 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Istex
|étape= Checkpoint
|type= RBID
|clé= ISTEX:FB225EC1B1A04F3D1F6E0D16830345C2AC4F1FBD
|texte= Genome analyses suggest the presence of polyploidy and recent human‐driven expansions in eight global populations of the honeybee pathogen Nosema ceranae
}}
| This area was generated with Dilib version V0.6.33. |  |