Clinical features and viral diagnosis of two cases of infection with Middle East Respiratory Syndrome coronavirus: a report of nosocomial transmission
Identifieur interne : 000001 ( Hal/Curation ); précédent : 000000; suivant : 000002Clinical features and viral diagnosis of two cases of infection with Middle East Respiratory Syndrome coronavirus: a report of nosocomial transmission
Auteurs : Benoit Guery [France] ; Julien Poissy [France] ; Loubna El Mansouf [France] ; Caroline Séjourné [France] ; Nicolas Ettahar [France] ; Xavier Lemaire [France] ; Fanny Vuotto [France] ; Anne Goffard [France] ; Sylvie Behillil [France] ; Vincent Enouf [France] ; Valerie Caro [France] ; Alexandra Mailles [France] ; Didier Che [France] ; Jean-Claude Manuguerra [France] ; Daniel Mathieu [France] ; Arnaud Fontanet [France] ; Sylvie Van Der Werf [France] ; The Mers-Cov GroupSource :
- The Lancet [ 0923-7577 ] ; 2013-06.
Abstract
Human infection with a novel coronavirus named Middle East Respiratory Syndrome coronavirus (MERS-CoV) was first identified in Saudi Arabia and the Middle East in September, 2012, with 44 laboratory-confirmed cases as of May 23, 2013. We report detailed clinical and virological data for two related cases of MERS-CoV disease, after nosocomial transmission of the virus from one patient to another in a French hospital.METHODS:Patient 1 visited Dubai in April, 2013; patient 2 lives in France and did not travel abroad. Both patients had underlying immunosuppressive disorders. We tested specimens from the upper (nasopharyngeal swabs) or the lower (bronchoalveolar lavage, sputum) respiratory tract and whole blood, plasma, and serum specimens for MERS-CoV by real-time RT-PCR targeting the upE and Orf1A genes of MERS-CoV.FINDINGS:Initial clinical presentation included fever, chills, and myalgia in both patients, and for patient 1, diarrhoea. Respiratory symptoms rapidly became predominant with acute respiratory failure leading to mechanical ventilation and extracorporeal membrane oxygenation (ECMO). Both patients developed acute renal failure. MERS-CoV was detected in lower respiratory tract specimens with high viral load (eg, cycle threshold [Ct] values of 22·9 for upE and 24 for Orf1a for a bronchoalveolar lavage sample from patient 1; Ct values of 22·5 for upE and 23·9 for Orf1a for an induced sputum sample from patient 2), whereas nasopharyngeal specimens were weakly positive or inconclusive. The two patients shared the same room for 3 days. The incubation period was estimated at 9-12 days for the second case. No secondary transmission was documented in hospital staff despite the absence of specific protective measures before the diagnosis of MERS-CoV was suspected. Patient 1 died on May 28, due to refractory multiple organ failure.INTERPRETATION:Patients with respiratory symptoms returning from the Middle East or exposed to a confirmed case should be isolated and investigated for MERS-CoV with lower respiratory tract sample analysis and an assumed incubation period of 12 days. Immunosuppression should also be taken into account as a risk factor.
Url:
DOI: 10.1016/S0140-6736(13)60982-4
Links toward previous steps (curation, corpus...)
- to stream Hal, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000001
Links to Exploration step
Hal:hal-02266768Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Clinical features and viral diagnosis of two cases of infection with Middle East Respiratory Syndrome coronavirus: a report of nosocomial transmission</title><author><name sortKey="Guery, Benoit" sort="Guery, Benoit" uniqKey="Guery B" first="Benoit" last="Guery">Benoit Guery</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-425779" status="VALID"> <orgName>Centre Hospitalier Régional Universitaire [Lille]</orgName> <orgName type="acronym">CHRU Lille</orgName> <desc> <address> <addrLine>2, avenue Oscar Lambret - 59037 Lille Cedex</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.chru-lille.fr/</ref> </desc> </hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Poissy, Julien" sort="Poissy, Julien" uniqKey="Poissy J" first="Julien" last="Poissy">Julien Poissy</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-425779" status="VALID"> <orgName>Centre Hospitalier Régional Universitaire [Lille]</orgName> <orgName type="acronym">CHRU Lille</orgName> <desc> <address> <addrLine>2, avenue Oscar Lambret - 59037 Lille Cedex</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.chru-lille.fr/</ref> </desc> </hal:affiliation><country>France</country></affiliation></author><author><name sortKey="El Mansouf, Loubna" sort="El Mansouf, Loubna" uniqKey="El Mansouf L" first="Loubna" last="El Mansouf">Loubna El Mansouf</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-458490" status="VALID"> <idno type="IdRef">032230222</idno> <orgName>Centre Hospitalier [Douai, Nord]</orgName> <desc> <address> <addrLine>Centre Hospitalier de Douai, Route de Cambrai - BP10740 - 59507 Douai cedex</addrLine> <country key="FR"></country> </address> </desc> </hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Sejourne, Caroline" sort="Sejourne, Caroline" uniqKey="Sejourne C" first="Caroline" last="Séjourné">Caroline Séjourné</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-458490" status="VALID"> <idno type="IdRef">032230222</idno> <orgName>Centre Hospitalier [Douai, Nord]</orgName> <desc> <address> <addrLine>Centre Hospitalier de Douai, Route de Cambrai - BP10740 - 59507 Douai cedex</addrLine> <country key="FR"></country> </address> </desc> </hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Ettahar, Nicolas" sort="Ettahar, Nicolas" uniqKey="Ettahar N" first="Nicolas" last="Ettahar">Nicolas Ettahar</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-556696" status="VALID"> <idno type="IdRef">032808747</idno> <orgName>Centre hospitalier [Valenciennes, Nord]</orgName> <desc> <address> <addrLine>Valenciennes</addrLine> <country key="FR"></country> </address> </desc> </hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Lemaire, Xavier" sort="Lemaire, Xavier" uniqKey="Lemaire X" first="Xavier" last="Lemaire">Xavier Lemaire</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-458490" status="VALID"> <idno type="IdRef">032230222</idno> <orgName>Centre Hospitalier [Douai, Nord]</orgName> <desc> <address> <addrLine>Centre Hospitalier de Douai, Route de Cambrai - BP10740 - 59507 Douai cedex</addrLine> <country key="FR"></country> </address> </desc> </hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Vuotto, Fanny" sort="Vuotto, Fanny" uniqKey="Vuotto F" first="Fanny" last="Vuotto">Fanny Vuotto</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-425779" status="VALID"> <orgName>Centre Hospitalier Régional Universitaire [Lille]</orgName> <orgName type="acronym">CHRU Lille</orgName> <desc> <address> <addrLine>2, avenue Oscar Lambret - 59037 Lille Cedex</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.chru-lille.fr/</ref> </desc> </hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Goffard, Anne" sort="Goffard, Anne" uniqKey="Goffard A" first="Anne" last="Goffard">Anne Goffard</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-425779" status="VALID"> <orgName>Centre Hospitalier Régional Universitaire [Lille]</orgName> <orgName type="acronym">CHRU Lille</orgName> <desc> <address> <addrLine>2, avenue Oscar Lambret - 59037 Lille Cedex</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.chru-lille.fr/</ref> </desc> </hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Behillil, Sylvie" sort="Behillil, Sylvie" uniqKey="Behillil S" first="Sylvie" last="Behillil">Sylvie Behillil</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-205198" status="OLD"> <orgName>Centre National de Référence des virus influenzae (Grippe)-Génétique moléculaire des virus à ARN</orgName> <orgName type="acronym">CNR</orgName> <desc> <address> <addrLine>25-28, rue du Docteur Roux 75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/fr/nrc/virus-influenzae/</ref> </desc> <listRelation> <relation active="#struct-300027" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-300027" type="direct"><org type="institution" xml:id="struct-300027" status="VALID"> <idno type="IdRef">027936643</idno> <idno type="ISNI">0000 0001 2353 6535</idno> <orgName>Institut Pasteur [Paris]</orgName> <date type="start">1887-06-04</date> <desc> <address> <addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://www.pasteur.fr</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Enouf, Vincent" sort="Enouf, Vincent" uniqKey="Enouf V" first="Vincent" last="Enouf">Vincent Enouf</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-205198" status="OLD"> <orgName>Centre National de Référence des virus influenzae (Grippe)-Génétique moléculaire des virus à ARN</orgName> <orgName type="acronym">CNR</orgName> <desc> <address> <addrLine>25-28, rue du Docteur Roux 75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/fr/nrc/virus-influenzae/</ref> </desc> <listRelation> <relation active="#struct-300027" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-300027" type="direct"><org type="institution" xml:id="struct-300027" status="VALID"> <idno type="IdRef">027936643</idno> <idno type="ISNI">0000 0001 2353 6535</idno> <orgName>Institut Pasteur [Paris]</orgName> <date type="start">1887-06-04</date> <desc> <address> <addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://www.pasteur.fr</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Caro, Valerie" sort="Caro, Valerie" uniqKey="Caro V" first="Valerie" last="Caro">Valerie Caro</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-31437" status="VALID"> <orgName>Cellule d'Intervention Biologique d'Urgence</orgName> <orgName type="acronym">CIBU</orgName> <desc> <address> <addrLine>Département Infection et Epidémiologie, 25-28 rue du Docteur Roux F-75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/en/nrc/laboratory-for-urgence-response-to-biological-threats/</ref> </desc> <listRelation> <relation active="#struct-300027" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-300027" type="direct"><org type="institution" xml:id="struct-300027" status="VALID"> <idno type="IdRef">027936643</idno> <idno type="ISNI">0000 0001 2353 6535</idno> <orgName>Institut Pasteur [Paris]</orgName> <date type="start">1887-06-04</date> <desc> <address> <addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://www.pasteur.fr</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Mailles, Alexandra" sort="Mailles, Alexandra" uniqKey="Mailles A" first="Alexandra" last="Mailles">Alexandra Mailles</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-300053" status="VALID"> <orgName>Institut de Veille Sanitaire </orgName> <orgName type="acronym">INVS</orgName> <desc> <address> <addrLine>12 rue du Val d'Osne, 94415 Saint Maurice Cedex, France</addrLine> <country key="FR"></country> </address> </desc> </hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Che, Didier" sort="Che, Didier" uniqKey="Che D" first="Didier" last="Che">Didier Che</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-300053" status="VALID"> <orgName>Institut de Veille Sanitaire </orgName> <orgName type="acronym">INVS</orgName> <desc> <address> <addrLine>12 rue du Val d'Osne, 94415 Saint Maurice Cedex, France</addrLine> <country key="FR"></country> </address> </desc> </hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Manuguerra, Jean Claude" sort="Manuguerra, Jean Claude" uniqKey="Manuguerra J" first="Jean-Claude" last="Manuguerra">Jean-Claude Manuguerra</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-31437" status="VALID"> <orgName>Cellule d'Intervention Biologique d'Urgence</orgName> <orgName type="acronym">CIBU</orgName> <desc> <address> <addrLine>Département Infection et Epidémiologie, 25-28 rue du Docteur Roux F-75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/en/nrc/laboratory-for-urgence-response-to-biological-threats/</ref> </desc> <listRelation> <relation active="#struct-300027" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-300027" type="direct"><org type="institution" xml:id="struct-300027" status="VALID"> <idno type="IdRef">027936643</idno> <idno type="ISNI">0000 0001 2353 6535</idno> <orgName>Institut Pasteur [Paris]</orgName> <date type="start">1887-06-04</date> <desc> <address> <addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://www.pasteur.fr</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Mathieu, Daniel" sort="Mathieu, Daniel" uniqKey="Mathieu D" first="Daniel" last="Mathieu">Daniel Mathieu</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-425779" status="VALID"> <orgName>Centre Hospitalier Régional Universitaire [Lille]</orgName> <orgName type="acronym">CHRU Lille</orgName> <desc> <address> <addrLine>2, avenue Oscar Lambret - 59037 Lille Cedex</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.chru-lille.fr/</ref> </desc> </hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Fontanet, Arnaud" sort="Fontanet, Arnaud" uniqKey="Fontanet A" first="Arnaud" last="Fontanet">Arnaud Fontanet</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-31480" status="VALID"> <orgName>Epidémiologie des Maladies Emergentes - Emerging Diseases Epidemiology</orgName> <desc> <address> <addrLine>Département Infection et Epidémiologie, 25-28 rue du Docteur Roux, F-75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/en/team/epidemiology-of-emerging-diseases/</ref> </desc> <listRelation> <relation active="#struct-507187" type="direct"></relation> <relation active="#struct-300027" type="indirect"></relation> <relation active="#struct-300351" type="indirect"></relation> </listRelation> <tutelles><tutelle active="#struct-507187" type="direct"><org type="regrouplaboratory" xml:id="struct-507187" status="VALID"> <orgName>Pasteur-Cnam risques infectieux et émergents</orgName> <orgName type="acronym">PACRI</orgName> <desc> <address> <addrLine>Paris</addrLine> <country key="FR"></country> </address> </desc> <listRelation> <relation active="#struct-300027" type="direct"></relation> <relation active="#struct-300351" type="direct"></relation> </listRelation> </org></tutelle><tutelle active="#struct-300027" type="indirect"><org type="institution" xml:id="struct-300027" status="VALID"> <idno type="IdRef">027936643</idno> <idno type="ISNI">0000 0001 2353 6535</idno> <orgName>Institut Pasteur [Paris]</orgName> <date type="start">1887-06-04</date> <desc> <address> <addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://www.pasteur.fr</ref> </desc> </org></tutelle><tutelle active="#struct-300351" type="indirect"><org type="institution" xml:id="struct-300351" status="VALID"> <idno type="IdRef">027404978</idno> <idno type="ISNI">0000000121690459</idno> <orgName>Conservatoire National des Arts et Métiers [CNAM]</orgName> <orgName type="acronym">CNAM</orgName> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnam.fr/</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Van Der Werf, Sylvie" sort="Van Der Werf, Sylvie" uniqKey="Van Der Werf S" first="Sylvie" last="Van Der Werf">Sylvie Van Der Werf</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-205198" status="OLD"> <orgName>Centre National de Référence des virus influenzae (Grippe)-Génétique moléculaire des virus à ARN</orgName> <orgName type="acronym">CNR</orgName> <desc> <address> <addrLine>25-28, rue du Docteur Roux 75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/fr/nrc/virus-influenzae/</ref> </desc> <listRelation> <relation active="#struct-300027" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-300027" type="direct"><org type="institution" xml:id="struct-300027" status="VALID"> <idno type="IdRef">027936643</idno> <idno type="ISNI">0000 0001 2353 6535</idno> <orgName>Institut Pasteur [Paris]</orgName> <date type="start">1887-06-04</date> <desc> <address> <addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://www.pasteur.fr</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Mers Cov Group, The" sort="Mers Cov Group, The" uniqKey="Mers Cov Group T" first="The" last="Mers-Cov Group">The Mers-Cov Group</name></author></titleStmt><publicationStmt><idno type="wicri:source">HAL</idno><idno type="RBID">Hal:hal-02266768</idno><idno type="halId">hal-02266768</idno><idno type="halUri">https://hal.archives-ouvertes.fr/hal-02266768</idno><idno type="url">https://hal.archives-ouvertes.fr/hal-02266768</idno><idno type="doi">10.1016/S0140-6736(13)60982-4</idno><date when="2013-06">2013-06</date><idno type="wicri:Area/Hal/Corpus">000001</idno><idno type="wicri:Area/Hal/Curation">000001</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Clinical features and viral diagnosis of two cases of infection with Middle East Respiratory Syndrome coronavirus: a report of nosocomial transmission</title><author><name sortKey="Guery, Benoit" sort="Guery, Benoit" uniqKey="Guery B" first="Benoit" last="Guery">Benoit Guery</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-425779" status="VALID"> <orgName>Centre Hospitalier Régional Universitaire [Lille]</orgName> <orgName type="acronym">CHRU Lille</orgName> <desc> <address> <addrLine>2, avenue Oscar Lambret - 59037 Lille Cedex</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.chru-lille.fr/</ref> </desc> </hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Poissy, Julien" sort="Poissy, Julien" uniqKey="Poissy J" first="Julien" last="Poissy">Julien Poissy</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-425779" status="VALID"> <orgName>Centre Hospitalier Régional Universitaire [Lille]</orgName> <orgName type="acronym">CHRU Lille</orgName> <desc> <address> <addrLine>2, avenue Oscar Lambret - 59037 Lille Cedex</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.chru-lille.fr/</ref> </desc> </hal:affiliation><country>France</country></affiliation></author><author><name sortKey="El Mansouf, Loubna" sort="El Mansouf, Loubna" uniqKey="El Mansouf L" first="Loubna" last="El Mansouf">Loubna El Mansouf</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-458490" status="VALID"> <idno type="IdRef">032230222</idno> <orgName>Centre Hospitalier [Douai, Nord]</orgName> <desc> <address> <addrLine>Centre Hospitalier de Douai, Route de Cambrai - BP10740 - 59507 Douai cedex</addrLine> <country key="FR"></country> </address> </desc> </hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Sejourne, Caroline" sort="Sejourne, Caroline" uniqKey="Sejourne C" first="Caroline" last="Séjourné">Caroline Séjourné</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-458490" status="VALID"> <idno type="IdRef">032230222</idno> <orgName>Centre Hospitalier [Douai, Nord]</orgName> <desc> <address> <addrLine>Centre Hospitalier de Douai, Route de Cambrai - BP10740 - 59507 Douai cedex</addrLine> <country key="FR"></country> </address> </desc> </hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Ettahar, Nicolas" sort="Ettahar, Nicolas" uniqKey="Ettahar N" first="Nicolas" last="Ettahar">Nicolas Ettahar</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-556696" status="VALID"> <idno type="IdRef">032808747</idno> <orgName>Centre hospitalier [Valenciennes, Nord]</orgName> <desc> <address> <addrLine>Valenciennes</addrLine> <country key="FR"></country> </address> </desc> </hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Lemaire, Xavier" sort="Lemaire, Xavier" uniqKey="Lemaire X" first="Xavier" last="Lemaire">Xavier Lemaire</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-458490" status="VALID"> <idno type="IdRef">032230222</idno> <orgName>Centre Hospitalier [Douai, Nord]</orgName> <desc> <address> <addrLine>Centre Hospitalier de Douai, Route de Cambrai - BP10740 - 59507 Douai cedex</addrLine> <country key="FR"></country> </address> </desc> </hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Vuotto, Fanny" sort="Vuotto, Fanny" uniqKey="Vuotto F" first="Fanny" last="Vuotto">Fanny Vuotto</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-425779" status="VALID"> <orgName>Centre Hospitalier Régional Universitaire [Lille]</orgName> <orgName type="acronym">CHRU Lille</orgName> <desc> <address> <addrLine>2, avenue Oscar Lambret - 59037 Lille Cedex</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.chru-lille.fr/</ref> </desc> </hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Goffard, Anne" sort="Goffard, Anne" uniqKey="Goffard A" first="Anne" last="Goffard">Anne Goffard</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-425779" status="VALID"> <orgName>Centre Hospitalier Régional Universitaire [Lille]</orgName> <orgName type="acronym">CHRU Lille</orgName> <desc> <address> <addrLine>2, avenue Oscar Lambret - 59037 Lille Cedex</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.chru-lille.fr/</ref> </desc> </hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Behillil, Sylvie" sort="Behillil, Sylvie" uniqKey="Behillil S" first="Sylvie" last="Behillil">Sylvie Behillil</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-205198" status="OLD"> <orgName>Centre National de Référence des virus influenzae (Grippe)-Génétique moléculaire des virus à ARN</orgName> <orgName type="acronym">CNR</orgName> <desc> <address> <addrLine>25-28, rue du Docteur Roux 75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/fr/nrc/virus-influenzae/</ref> </desc> <listRelation> <relation active="#struct-300027" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-300027" type="direct"><org type="institution" xml:id="struct-300027" status="VALID"> <idno type="IdRef">027936643</idno> <idno type="ISNI">0000 0001 2353 6535</idno> <orgName>Institut Pasteur [Paris]</orgName> <date type="start">1887-06-04</date> <desc> <address> <addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://www.pasteur.fr</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Enouf, Vincent" sort="Enouf, Vincent" uniqKey="Enouf V" first="Vincent" last="Enouf">Vincent Enouf</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-205198" status="OLD"> <orgName>Centre National de Référence des virus influenzae (Grippe)-Génétique moléculaire des virus à ARN</orgName> <orgName type="acronym">CNR</orgName> <desc> <address> <addrLine>25-28, rue du Docteur Roux 75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/fr/nrc/virus-influenzae/</ref> </desc> <listRelation> <relation active="#struct-300027" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-300027" type="direct"><org type="institution" xml:id="struct-300027" status="VALID"> <idno type="IdRef">027936643</idno> <idno type="ISNI">0000 0001 2353 6535</idno> <orgName>Institut Pasteur [Paris]</orgName> <date type="start">1887-06-04</date> <desc> <address> <addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://www.pasteur.fr</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Caro, Valerie" sort="Caro, Valerie" uniqKey="Caro V" first="Valerie" last="Caro">Valerie Caro</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-31437" status="VALID"> <orgName>Cellule d'Intervention Biologique d'Urgence</orgName> <orgName type="acronym">CIBU</orgName> <desc> <address> <addrLine>Département Infection et Epidémiologie, 25-28 rue du Docteur Roux F-75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/en/nrc/laboratory-for-urgence-response-to-biological-threats/</ref> </desc> <listRelation> <relation active="#struct-300027" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-300027" type="direct"><org type="institution" xml:id="struct-300027" status="VALID"> <idno type="IdRef">027936643</idno> <idno type="ISNI">0000 0001 2353 6535</idno> <orgName>Institut Pasteur [Paris]</orgName> <date type="start">1887-06-04</date> <desc> <address> <addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://www.pasteur.fr</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Mailles, Alexandra" sort="Mailles, Alexandra" uniqKey="Mailles A" first="Alexandra" last="Mailles">Alexandra Mailles</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-300053" status="VALID"> <orgName>Institut de Veille Sanitaire </orgName> <orgName type="acronym">INVS</orgName> <desc> <address> <addrLine>12 rue du Val d'Osne, 94415 Saint Maurice Cedex, France</addrLine> <country key="FR"></country> </address> </desc> </hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Che, Didier" sort="Che, Didier" uniqKey="Che D" first="Didier" last="Che">Didier Che</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-300053" status="VALID"> <orgName>Institut de Veille Sanitaire </orgName> <orgName type="acronym">INVS</orgName> <desc> <address> <addrLine>12 rue du Val d'Osne, 94415 Saint Maurice Cedex, France</addrLine> <country key="FR"></country> </address> </desc> </hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Manuguerra, Jean Claude" sort="Manuguerra, Jean Claude" uniqKey="Manuguerra J" first="Jean-Claude" last="Manuguerra">Jean-Claude Manuguerra</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-31437" status="VALID"> <orgName>Cellule d'Intervention Biologique d'Urgence</orgName> <orgName type="acronym">CIBU</orgName> <desc> <address> <addrLine>Département Infection et Epidémiologie, 25-28 rue du Docteur Roux F-75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/en/nrc/laboratory-for-urgence-response-to-biological-threats/</ref> </desc> <listRelation> <relation active="#struct-300027" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-300027" type="direct"><org type="institution" xml:id="struct-300027" status="VALID"> <idno type="IdRef">027936643</idno> <idno type="ISNI">0000 0001 2353 6535</idno> <orgName>Institut Pasteur [Paris]</orgName> <date type="start">1887-06-04</date> <desc> <address> <addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://www.pasteur.fr</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Mathieu, Daniel" sort="Mathieu, Daniel" uniqKey="Mathieu D" first="Daniel" last="Mathieu">Daniel Mathieu</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-425779" status="VALID"> <orgName>Centre Hospitalier Régional Universitaire [Lille]</orgName> <orgName type="acronym">CHRU Lille</orgName> <desc> <address> <addrLine>2, avenue Oscar Lambret - 59037 Lille Cedex</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.chru-lille.fr/</ref> </desc> </hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Fontanet, Arnaud" sort="Fontanet, Arnaud" uniqKey="Fontanet A" first="Arnaud" last="Fontanet">Arnaud Fontanet</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-31480" status="VALID"> <orgName>Epidémiologie des Maladies Emergentes - Emerging Diseases Epidemiology</orgName> <desc> <address> <addrLine>Département Infection et Epidémiologie, 25-28 rue du Docteur Roux, F-75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/en/team/epidemiology-of-emerging-diseases/</ref> </desc> <listRelation> <relation active="#struct-507187" type="direct"></relation> <relation active="#struct-300027" type="indirect"></relation> <relation active="#struct-300351" type="indirect"></relation> </listRelation> <tutelles><tutelle active="#struct-507187" type="direct"><org type="regrouplaboratory" xml:id="struct-507187" status="VALID"> <orgName>Pasteur-Cnam risques infectieux et émergents</orgName> <orgName type="acronym">PACRI</orgName> <desc> <address> <addrLine>Paris</addrLine> <country key="FR"></country> </address> </desc> <listRelation> <relation active="#struct-300027" type="direct"></relation> <relation active="#struct-300351" type="direct"></relation> </listRelation> </org></tutelle><tutelle active="#struct-300027" type="indirect"><org type="institution" xml:id="struct-300027" status="VALID"> <idno type="IdRef">027936643</idno> <idno type="ISNI">0000 0001 2353 6535</idno> <orgName>Institut Pasteur [Paris]</orgName> <date type="start">1887-06-04</date> <desc> <address> <addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://www.pasteur.fr</ref> </desc> </org></tutelle><tutelle active="#struct-300351" type="indirect"><org type="institution" xml:id="struct-300351" status="VALID"> <idno type="IdRef">027404978</idno> <idno type="ISNI">0000000121690459</idno> <orgName>Conservatoire National des Arts et Métiers [CNAM]</orgName> <orgName type="acronym">CNAM</orgName> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnam.fr/</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Van Der Werf, Sylvie" sort="Van Der Werf, Sylvie" uniqKey="Van Der Werf S" first="Sylvie" last="Van Der Werf">Sylvie Van Der Werf</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-205198" status="OLD"> <orgName>Centre National de Référence des virus influenzae (Grippe)-Génétique moléculaire des virus à ARN</orgName> <orgName type="acronym">CNR</orgName> <desc> <address> <addrLine>25-28, rue du Docteur Roux 75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/fr/nrc/virus-influenzae/</ref> </desc> <listRelation> <relation active="#struct-300027" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-300027" type="direct"><org type="institution" xml:id="struct-300027" status="VALID"> <idno type="IdRef">027936643</idno> <idno type="ISNI">0000 0001 2353 6535</idno> <orgName>Institut Pasteur [Paris]</orgName> <date type="start">1887-06-04</date> <desc> <address> <addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://www.pasteur.fr</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Mers Cov Group, The" sort="Mers Cov Group, The" uniqKey="Mers Cov Group T" first="The" last="Mers-Cov Group">The Mers-Cov Group</name></author></analytic><idno type="DOI">10.1016/S0140-6736(13)60982-4</idno><series><title level="j">The Lancet</title><idno type="ISSN">0923-7577</idno><imprint><date type="datePub">2013-06</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"> <p>Human infection with a novel coronavirus named Middle East Respiratory Syndrome coronavirus (MERS-CoV) was first identified in Saudi Arabia and the Middle East in September, 2012, with 44 laboratory-confirmed cases as of May 23, 2013. We report detailed clinical and virological data for two related cases of MERS-CoV disease, after nosocomial transmission of the virus from one patient to another in a French hospital.METHODS:Patient 1 visited Dubai in April, 2013; patient 2 lives in France and did not travel abroad. Both patients had underlying immunosuppressive disorders. We tested specimens from the upper (nasopharyngeal swabs) or the lower (bronchoalveolar lavage, sputum) respiratory tract and whole blood, plasma, and serum specimens for MERS-CoV by real-time RT-PCR targeting the upE and Orf1A genes of MERS-CoV.FINDINGS:Initial clinical presentation included fever, chills, and myalgia in both patients, and for patient 1, diarrhoea. Respiratory symptoms rapidly became predominant with acute respiratory failure leading to mechanical ventilation and extracorporeal membrane oxygenation (ECMO). Both patients developed acute renal failure. MERS-CoV was detected in lower respiratory tract specimens with high viral load (eg, cycle threshold [Ct] values of 22·9 for upE and 24 for Orf1a for a bronchoalveolar lavage sample from patient 1; Ct values of 22·5 for upE and 23·9 for Orf1a for an induced sputum sample from patient 2), whereas nasopharyngeal specimens were weakly positive or inconclusive. The two patients shared the same room for 3 days. The incubation period was estimated at 9-12 days for the second case. No secondary transmission was documented in hospital staff despite the absence of specific protective measures before the diagnosis of MERS-CoV was suspected. Patient 1 died on May 28, due to refractory multiple organ failure.INTERPRETATION:Patients with respiratory symptoms returning from the Middle East or exposed to a confirmed case should be isolated and investigated for MERS-CoV with lower respiratory tract sample analysis and an assumed incubation period of 12 days. Immunosuppression should also be taken into account as a risk factor.</p> </div></front></TEI><hal api="V3"> <titleStmt> <title xml:lang="en">Clinical features and viral diagnosis of two cases of infection with Middle East Respiratory Syndrome coronavirus: a report of nosocomial transmission</title> <author role="aut"> <persName> <forename type="first">Benoit</forename> <surname>Guery</surname> </persName> <idno type="halauthorid">178418</idno> <affiliation ref="#struct-425779"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Julien</forename> <surname>Poissy</surname> </persName> <idno type="halauthorid">1108614</idno> <affiliation ref="#struct-425779"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Loubna</forename> <surname>el Mansouf</surname> </persName> <idno type="halauthorid">11555292</idno> <affiliation ref="#struct-458490"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Caroline</forename> <surname>Séjourné</surname> </persName> <idno type="halauthorid">11555293</idno> <affiliation ref="#struct-458490"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Nicolas</forename> <surname>Ettahar</surname> </persName> <idno type="halauthorid">11555294</idno> <affiliation ref="#struct-556696"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Xavier</forename> <surname>Lemaire</surname> </persName> <idno type="halauthorid">291555</idno> <affiliation ref="#struct-458490"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Fanny</forename> <surname>Vuotto</surname> </persName> <email type="md5">e76787c14b46eaef3deb07e6af28105c</email> <email type="domain">free.fr</email> <idno type="halauthorid">645038</idno> <affiliation ref="#struct-425779"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Anne</forename> <surname>Goffard</surname> </persName> <idno type="halauthorid">136784</idno> <affiliation ref="#struct-425779"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Sylvie</forename> <surname>Behillil</surname> </persName> <idno type="halauthorid">1214781</idno> <affiliation ref="#struct-205198"></affiliation> <affiliation ref="#struct-481195"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Vincent</forename> <surname>Enouf</surname> </persName> <idno type="halauthorid">400918</idno> <affiliation ref="#struct-205198"></affiliation> <affiliation ref="#struct-481195"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Valerie</forename> <surname>Caro</surname> </persName> <idno type="halauthorid">204629</idno> <affiliation ref="#struct-31437"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Alexandra</forename> <surname>Mailles</surname> </persName> <email type="md5">75de7164585cdda503ebd9fa8762aec3</email> <email type="domain">invs.sante.fr</email> <idno type="halauthorid">901981</idno> <affiliation ref="#struct-300053"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Didier</forename> <surname>Che</surname> </persName> <idno type="halauthorid">570807</idno> <affiliation ref="#struct-300053"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Jean-Claude</forename> <surname>Manuguerra</surname> </persName> <idno type="halauthorid">202084</idno> <affiliation ref="#struct-31437"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Daniel</forename> <surname>Mathieu</surname> </persName> <idno type="halauthorid">363191</idno> <affiliation ref="#struct-425779"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Arnaud</forename> <surname>Fontanet</surname> </persName> <idno type="halauthorid">117989</idno> <affiliation ref="#struct-31480"></affiliation> <affiliation ref="#struct-300351"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Sylvie</forename> <surname>Van Der Werf</surname> </persName> <idno type="halauthorid">202082</idno> <affiliation ref="#struct-205198"></affiliation> <affiliation ref="#struct-481195"></affiliation> </author> <author role="aut"> <persName> <forename type="first">the</forename> <surname>MERS-CoV group</surname> </persName> <idno type="halauthorid">11664481</idno> </author> <editor role="depositor"> <persName> <forename>Anne</forename> <surname>Goffard</surname> </persName> <email type="md5">1f1f6670bfbb3e6b1ec674437578a5f3</email> <email type="domain">univ-lille.fr</email> </editor> <funder ref="#projanr-37850"></funder> <funder ref="#projeurop-18734"></funder> <funder ref="#projeurop-89460"></funder> <funder>The National Reference Center for Influenza Viruses and the CIBU are supported in part by funds from the French Institute for Public Health Surveillance (Institut de veille sanitaire, InVS). Work related to collection of data, implementation of the assays, and sequencing was done with funds from ANR grant Labex Integrative Biology of Emerging Infectious Diseases (IBEID, grant number ANR-10-LABX-62-IBEID from the French Government's Investissement d'Avenir programme) and from the European Community's Seventh Framework Programme (FP7/2007–2013) under the project EMPERIE (European Community grant agreement number 223498) and under the project PREDEMICS (grant agreement number 278433)</funder> </titleStmt> <editionStmt> <edition n="v1" type="current"> <date type="whenSubmitted">2019-08-16 10:15:27</date> <date type="whenModified">2020-04-20 14:46:02</date> <date type="whenReleased">2019-08-16 10:15:27</date> <date type="whenProduced">2013-06</date> <ref type="externalLink" target="http://www.thelancet.com/article/S0140673613609824/pdf"></ref> </edition> <respStmt> <resp>contributor</resp> <name key="594167"> <persName> <forename>Anne</forename> <surname>Goffard</surname> </persName> <email type="md5">1f1f6670bfbb3e6b1ec674437578a5f3</email> <email type="domain">univ-lille.fr</email> </name> </respStmt> </editionStmt> <publicationStmt> <distributor>CCSD</distributor> <idno type="halId">hal-02266768</idno> <idno type="halUri">https://hal.archives-ouvertes.fr/hal-02266768</idno> <idno type="halBibtex">guery:hal-02266768</idno> <idno type="halRefHtml">The Lancet, Elsevier, 2013, 381 (9885), pp.2265-2272. ⟨10.1016/S0140-6736(13)60982-4⟩</idno> <idno type="halRef">The Lancet, Elsevier, 2013, 381 (9885), pp.2265-2272. ⟨10.1016/S0140-6736(13)60982-4⟩</idno> </publicationStmt> <seriesStmt> <idno type="stamp" n="INVS">Institut de veille sanitaire</idno> <idno type="stamp" n="CNAM">Conservatoire National des Arts et Métiers</idno> <idno type="stamp" n="UNIV-HESAM-CNAM" corresp="UNIV-HESAM">Conservatoire National des Arts et Métiers</idno> <idno type="stamp" n="UNIV-PARIS7" corresp="UNIV-PARIS">Université Denis Diderot - Paris VII</idno> <idno type="stamp" n="USPC">Université Sorbonne Paris Cité</idno> <idno type="stamp" n="UNIV-HESAM">HESAM Université</idno> <idno type="stamp" n="PASTEUR">Institut Pasteur</idno> <idno type="stamp" n="OPENAIRE">OpenAIRE</idno> <idno type="stamp" n="UNIV-PARIS">Université de Paris</idno> <idno type="stamp" n="UP-SCIENCES">Université de Paris - Faculté des Sciences</idno> </seriesStmt> <notesStmt> <note type="commentary">Comment in: Middle East respiratory syndrome: new disease, old lessons. [Lancet. 2013]</note> <note type="audience" n="2">International</note> <note type="popular" n="0">No</note> <note type="peer" n="1">Yes</note> </notesStmt> <sourceDesc> <biblStruct> <analytic> <title xml:lang="en">Clinical features and viral diagnosis of two cases of infection with Middle East Respiratory Syndrome coronavirus: a report of nosocomial transmission</title> <author role="aut"> <persName> <forename type="first">Benoit</forename> <surname>Guery</surname> </persName> <idno type="halauthorid">178418</idno> <affiliation ref="#struct-425779"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Julien</forename> <surname>Poissy</surname> </persName> <idno type="halauthorid">1108614</idno> <affiliation ref="#struct-425779"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Loubna</forename> <surname>el Mansouf</surname> </persName> <idno type="halauthorid">11555292</idno> <affiliation ref="#struct-458490"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Caroline</forename> <surname>Séjourné</surname> </persName> <idno type="halauthorid">11555293</idno> <affiliation ref="#struct-458490"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Nicolas</forename> <surname>Ettahar</surname> </persName> <idno type="halauthorid">11555294</idno> <affiliation ref="#struct-556696"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Xavier</forename> <surname>Lemaire</surname> </persName> <idno type="halauthorid">291555</idno> <affiliation ref="#struct-458490"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Fanny</forename> <surname>Vuotto</surname> </persName> <email type="md5">e76787c14b46eaef3deb07e6af28105c</email> <email type="domain">free.fr</email> <idno type="halauthorid">645038</idno> <affiliation ref="#struct-425779"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Anne</forename> <surname>Goffard</surname> </persName> <idno type="halauthorid">136784</idno> <affiliation ref="#struct-425779"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Sylvie</forename> <surname>Behillil</surname> </persName> <idno type="halauthorid">1214781</idno> <affiliation ref="#struct-205198"></affiliation> <affiliation ref="#struct-481195"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Vincent</forename> <surname>Enouf</surname> </persName> <idno type="halauthorid">400918</idno> <affiliation ref="#struct-205198"></affiliation> <affiliation ref="#struct-481195"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Valerie</forename> <surname>Caro</surname> </persName> <idno type="halauthorid">204629</idno> <affiliation ref="#struct-31437"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Alexandra</forename> <surname>Mailles</surname> </persName> <email type="md5">75de7164585cdda503ebd9fa8762aec3</email> <email type="domain">invs.sante.fr</email> <idno type="halauthorid">901981</idno> <affiliation ref="#struct-300053"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Didier</forename> <surname>Che</surname> </persName> <idno type="halauthorid">570807</idno> <affiliation ref="#struct-300053"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Jean-Claude</forename> <surname>Manuguerra</surname> </persName> <idno type="halauthorid">202084</idno> <affiliation ref="#struct-31437"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Daniel</forename> <surname>Mathieu</surname> </persName> <idno type="halauthorid">363191</idno> <affiliation ref="#struct-425779"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Arnaud</forename> <surname>Fontanet</surname> </persName> <idno type="halauthorid">117989</idno> <affiliation ref="#struct-31480"></affiliation> <affiliation ref="#struct-300351"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Sylvie</forename> <surname>Van Der Werf</surname> </persName> <idno type="halauthorid">202082</idno> <affiliation ref="#struct-205198"></affiliation> <affiliation ref="#struct-481195"></affiliation> </author> <author role="aut"> <persName> <forename type="first">the</forename> <surname>MERS-CoV group</surname> </persName> <idno type="halauthorid">11664481</idno> </author> </analytic> <monogr> <idno type="halJournalId" status="VALID">26611</idno> <idno type="issn">0923-7577</idno> <title level="j">The Lancet</title> <imprint> <publisher>Elsevier</publisher> <biblScope unit="volume">381</biblScope> <biblScope unit="issue">9885</biblScope> <biblScope unit="pp">2265-2272</biblScope> <date type="datePub">2013-06</date> </imprint> </monogr> <idno type="doi">10.1016/S0140-6736(13)60982-4</idno> <idno type="pubmed">23727167</idno> </biblStruct> </sourceDesc> <profileDesc> <langUsage> <language ident="en">English</language> </langUsage> <textClass> <classCode scheme="halDomain" n="sdv">Life Sciences [q-bio]</classCode> <classCode scheme="halDomain" n="sdv.mp.vir">Life Sciences [q-bio]/Microbiology and Parasitology/Virology</classCode> <classCode scheme="halDomain" n="sdv.mhep.me">Life Sciences [q-bio]/Human health and pathology/Emerging diseases</classCode> <classCode scheme="halDomain" n="sdv.mhep.mi">Life Sciences [q-bio]/Human health and pathology/Infectious diseases</classCode> <classCode scheme="halTypology" n="ART">Journal articles</classCode> </textClass> <abstract xml:lang="en"> <p>Human infection with a novel coronavirus named Middle East Respiratory Syndrome coronavirus (MERS-CoV) was first identified in Saudi Arabia and the Middle East in September, 2012, with 44 laboratory-confirmed cases as of May 23, 2013. We report detailed clinical and virological data for two related cases of MERS-CoV disease, after nosocomial transmission of the virus from one patient to another in a French hospital.METHODS:Patient 1 visited Dubai in April, 2013; patient 2 lives in France and did not travel abroad. Both patients had underlying immunosuppressive disorders. We tested specimens from the upper (nasopharyngeal swabs) or the lower (bronchoalveolar lavage, sputum) respiratory tract and whole blood, plasma, and serum specimens for MERS-CoV by real-time RT-PCR targeting the upE and Orf1A genes of MERS-CoV.FINDINGS:Initial clinical presentation included fever, chills, and myalgia in both patients, and for patient 1, diarrhoea. Respiratory symptoms rapidly became predominant with acute respiratory failure leading to mechanical ventilation and extracorporeal membrane oxygenation (ECMO). Both patients developed acute renal failure. MERS-CoV was detected in lower respiratory tract specimens with high viral load (eg, cycle threshold [Ct] values of 22·9 for upE and 24 for Orf1a for a bronchoalveolar lavage sample from patient 1; Ct values of 22·5 for upE and 23·9 for Orf1a for an induced sputum sample from patient 2), whereas nasopharyngeal specimens were weakly positive or inconclusive. The two patients shared the same room for 3 days. The incubation period was estimated at 9-12 days for the second case. No secondary transmission was documented in hospital staff despite the absence of specific protective measures before the diagnosis of MERS-CoV was suspected. Patient 1 died on May 28, due to refractory multiple organ failure.INTERPRETATION:Patients with respiratory symptoms returning from the Middle East or exposed to a confirmed case should be isolated and investigated for MERS-CoV with lower respiratory tract sample analysis and an assumed incubation period of 12 days. Immunosuppression should also be taken into account as a risk factor.</p> </abstract> <particDesc> <org type="consortium">We thank the nurses and all health-care workers from the hospitals in Valenciennes, Douai, and Lille who, in this crisis situation, took care of the patients and controlled the spread of this disease. We thank P Despres, S Paulous, and M P Frenkiel (Unit of Flavivirus-Host Molecular Interactions, Institut Pasteur) for preliminary serological analyses. We thank Eric Kipnis for editing of the report.</org> </particDesc> </profileDesc> </hal></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Hal/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000001 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Hal/Curation/biblio.hfd -nk 000001 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Hal
|étape= Curation
|type= RBID
|clé= Hal:hal-02266768
|texte= Clinical features and viral diagnosis of two cases of infection with Middle East Respiratory Syndrome coronavirus: a report of nosocomial transmission
}}
| This area was generated with Dilib version V0.6.33. |  |