Toward the identification of viral cap-methyltransferase inhibitors by fluorescence screening assay
Identifieur interne : 000022 ( Hal/Corpus ); précédent : 000021; suivant : 000023Toward the identification of viral cap-methyltransferase inhibitors by fluorescence screening assay
Auteurs : Wahiba Aouadi ; C. Ecilia Eydoux ; Bruno Coutard ; Baptiste Martin ; Françoise Debart ; Jean Jacques Vasseur ; Jean Contreras ; Christophe Morice ; Gilles Quérat ; Marie-Louise Jung ; Bruno Canard ; Jean-Claude Guillemot ; Etienne DecrolySource :
- Antiviral Research [ 0166-3542 ] ; 2017-08.
English descriptors
- mix :
Abstract
Two highly pathogenic human coronaviruses associated with severe respiratory syndromes emerged since the beginning of the century. The severe acute respiratory syndrome SARS-coronavirus (CoV) spread first in southern China in 2003 with about 8000 infected cases in few months. Then in 2012, the Middle East respiratory syndrome (MERS-CoV) emerged from the Arabian Peninsula giving a still ongoing epidemic associated to a high fatality rate. CoVs are thus considered a major health threat. This is especially true as no vaccine nor specific therapeutic are available against either SARS-or MERS-CoV. Therefore, new drugs need to be identified in order to develop antiviral treatments limiting CoV repli-cation. In this study, we focus on the nsp14 protein, which plays a key role in virus replication as it methylates the RNA cap structure at the N7 position of the guanine. We developed a high-throughput N7-MTase assay based on Homogenous Time Resolved Fluorescence (HTRF ®) and screened chemical libraries (2000 compounds) on the SARS-CoV nsp14. 20 compounds inhibiting the SARS-CoV nsp14 were further evaluated by IC 50 determination and their specificity was assessed toward flavivirus-and human cap N7-MTases. Our results reveal three classes of compounds: 1) molecules inhibiting several MTases as well as the dengue virus polymerase activity unspecifically, 2) pan MTases inhibitors targeting both viral and cellular MTases, and 3) inhibitors targeting one viral MTase more specifically showing however activity against the human cap N7-MTase. These compounds provide a first basis towards the development of more specific inhibitors of viral methyltransferases.
Url:
DOI: 10.1016/j.antiviral.2017.06.021
Links to Exploration step
Hal:hal-02349530Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Toward the identification of viral cap-methyltransferase inhibitors by fluorescence screening assay</title>
<author><name sortKey="Aouadi, Wahiba" sort="Aouadi, Wahiba" uniqKey="Aouadi W" first="Wahiba" last="Aouadi">Wahiba Aouadi</name>
<affiliation><hal:affiliation type="laboratory" xml:id="struct-199349" status="OLD"> <idno type="IdRef">149981651</idno>
<idno type="RNSR">201220260Z</idno>
<orgName>Architecture et fonction des macromolécules biologiques</orgName>
<orgName type="acronym">AFMB</orgName>
<date type="start">2012-01-01</date>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.afmb.univ-mrs.fr/</ref>
</desc>
<listRelation> <relation name="UMR7257" active="#struct-92114" type="direct"></relation>
<relation name="UMR7257" active="#struct-198056" type="direct"></relation>
<relation name="UMR7257" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle name="UMR7257" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="OLD"> <orgName>Institut National de la Recherche Agronomique</orgName>
<orgName type="acronym">INRA</orgName>
<date type="start">1946-05-18</date>
<date type="end">2019-12-31</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.inra.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7257" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno>
<idno type="ISNI">0000 0001 2176 4817</idno>
<orgName>Aix Marseille Université</orgName>
<orgName type="acronym">AMU</orgName>
<date type="start">2012-01-01</date>
<desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-amu.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7257" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="ISNI">0000000122597504</idno>
<idno type="IdRef">02636817X</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author><name sortKey="Ecilia Eydoux, C" sort="Ecilia Eydoux, C" uniqKey="Ecilia Eydoux C" first="C" last="Ecilia Eydoux">C. Ecilia Eydoux</name>
<affiliation><hal:affiliation type="laboratory" xml:id="struct-199349" status="OLD"> <idno type="IdRef">149981651</idno>
<idno type="RNSR">201220260Z</idno>
<orgName>Architecture et fonction des macromolécules biologiques</orgName>
<orgName type="acronym">AFMB</orgName>
<date type="start">2012-01-01</date>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.afmb.univ-mrs.fr/</ref>
</desc>
<listRelation> <relation name="UMR7257" active="#struct-92114" type="direct"></relation>
<relation name="UMR7257" active="#struct-198056" type="direct"></relation>
<relation name="UMR7257" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle name="UMR7257" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="OLD"> <orgName>Institut National de la Recherche Agronomique</orgName>
<orgName type="acronym">INRA</orgName>
<date type="start">1946-05-18</date>
<date type="end">2019-12-31</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.inra.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7257" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno>
<idno type="ISNI">0000 0001 2176 4817</idno>
<orgName>Aix Marseille Université</orgName>
<orgName type="acronym">AMU</orgName>
<date type="start">2012-01-01</date>
<desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-amu.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7257" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="ISNI">0000000122597504</idno>
<idno type="IdRef">02636817X</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author><name sortKey="Coutard, Bruno" sort="Coutard, Bruno" uniqKey="Coutard B" first="Bruno" last="Coutard">Bruno Coutard</name>
<affiliation><hal:affiliation type="laboratory" xml:id="struct-199349" status="OLD"> <idno type="IdRef">149981651</idno>
<idno type="RNSR">201220260Z</idno>
<orgName>Architecture et fonction des macromolécules biologiques</orgName>
<orgName type="acronym">AFMB</orgName>
<date type="start">2012-01-01</date>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.afmb.univ-mrs.fr/</ref>
</desc>
<listRelation> <relation name="UMR7257" active="#struct-92114" type="direct"></relation>
<relation name="UMR7257" active="#struct-198056" type="direct"></relation>
<relation name="UMR7257" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle name="UMR7257" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="OLD"> <orgName>Institut National de la Recherche Agronomique</orgName>
<orgName type="acronym">INRA</orgName>
<date type="start">1946-05-18</date>
<date type="end">2019-12-31</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.inra.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7257" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno>
<idno type="ISNI">0000 0001 2176 4817</idno>
<orgName>Aix Marseille Université</orgName>
<orgName type="acronym">AMU</orgName>
<date type="start">2012-01-01</date>
<desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-amu.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7257" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="ISNI">0000000122597504</idno>
<idno type="IdRef">02636817X</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author><name sortKey="Martin, Baptiste" sort="Martin, Baptiste" uniqKey="Martin B" first="Baptiste" last="Martin">Baptiste Martin</name>
<affiliation><hal:affiliation type="laboratory" xml:id="struct-199349" status="OLD"> <idno type="IdRef">149981651</idno>
<idno type="RNSR">201220260Z</idno>
<orgName>Architecture et fonction des macromolécules biologiques</orgName>
<orgName type="acronym">AFMB</orgName>
<date type="start">2012-01-01</date>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.afmb.univ-mrs.fr/</ref>
</desc>
<listRelation> <relation name="UMR7257" active="#struct-92114" type="direct"></relation>
<relation name="UMR7257" active="#struct-198056" type="direct"></relation>
<relation name="UMR7257" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle name="UMR7257" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="OLD"> <orgName>Institut National de la Recherche Agronomique</orgName>
<orgName type="acronym">INRA</orgName>
<date type="start">1946-05-18</date>
<date type="end">2019-12-31</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.inra.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7257" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno>
<idno type="ISNI">0000 0001 2176 4817</idno>
<orgName>Aix Marseille Université</orgName>
<orgName type="acronym">AMU</orgName>
<date type="start">2012-01-01</date>
<desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-amu.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7257" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="ISNI">0000000122597504</idno>
<idno type="IdRef">02636817X</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author><name sortKey="Debart, Francoise" sort="Debart, Francoise" uniqKey="Debart F" first="Françoise" last="Debart">Françoise Debart</name>
<affiliation><hal:affiliation type="laboratory" xml:id="struct-24488" status="VALID"> <idno type="RNSR">200711912X</idno>
<orgName>Institut des Biomolécules Max Mousseron [Pôle Chimie Balard]</orgName>
<orgName type="acronym">IBMM</orgName>
<desc> <address> <addrLine>Faculté de Pharmacie - 15 Av. Charles Flahault - BP 14 491 - 34093 Montpellier Cedex 5</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.ibmm.univ-montp1.fr</ref>
</desc>
<listRelation> <relation active="#struct-300370" type="direct"></relation>
<relation active="#struct-410122" type="direct"></relation>
<relation name="UMR5247" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-300370" type="direct"><org type="institution" xml:id="struct-300370" status="VALID"> <orgName>Ecole Nationale Supérieure de Chimie de Montpellier</orgName>
<orgName type="acronym">ENSCM</orgName>
<desc> <address> <addrLine>8 Rue de l'Ecole Normale 34296 Montpellier Cedex 5</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.enscm.fr</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-410122" type="direct"><org type="institution" xml:id="struct-410122" status="VALID"> <idno type="ISNI">0000000120970141</idno>
<orgName>Université de Montpellier</orgName>
<orgName type="acronym">UM</orgName>
<desc> <address> <addrLine>163 rue Auguste Broussonnet - 34090 Montpellier</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.umontpellier.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR5247" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="ISNI">0000000122597504</idno>
<idno type="IdRef">02636817X</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author><name sortKey="Jacques Vasseur, Jean" sort="Jacques Vasseur, Jean" uniqKey="Jacques Vasseur J" first="Jean" last="Jacques Vasseur">Jean Jacques Vasseur</name>
<affiliation><hal:affiliation type="laboratory" xml:id="struct-24488" status="VALID"> <idno type="RNSR">200711912X</idno>
<orgName>Institut des Biomolécules Max Mousseron [Pôle Chimie Balard]</orgName>
<orgName type="acronym">IBMM</orgName>
<desc> <address> <addrLine>Faculté de Pharmacie - 15 Av. Charles Flahault - BP 14 491 - 34093 Montpellier Cedex 5</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.ibmm.univ-montp1.fr</ref>
</desc>
<listRelation> <relation active="#struct-300370" type="direct"></relation>
<relation active="#struct-410122" type="direct"></relation>
<relation name="UMR5247" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-300370" type="direct"><org type="institution" xml:id="struct-300370" status="VALID"> <orgName>Ecole Nationale Supérieure de Chimie de Montpellier</orgName>
<orgName type="acronym">ENSCM</orgName>
<desc> <address> <addrLine>8 Rue de l'Ecole Normale 34296 Montpellier Cedex 5</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.enscm.fr</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-410122" type="direct"><org type="institution" xml:id="struct-410122" status="VALID"> <idno type="ISNI">0000000120970141</idno>
<orgName>Université de Montpellier</orgName>
<orgName type="acronym">UM</orgName>
<desc> <address> <addrLine>163 rue Auguste Broussonnet - 34090 Montpellier</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.umontpellier.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR5247" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="ISNI">0000000122597504</idno>
<idno type="IdRef">02636817X</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author><name sortKey="Contreras, Jean" sort="Contreras, Jean" uniqKey="Contreras J" first="Jean" last="Contreras">Jean Contreras</name>
<affiliation><hal:affiliation type="institution" xml:id="struct-553076" status="INCOMING"> <orgName>Prestwick Chemical [Illkirch, Strasbourg]</orgName>
<desc> <address> <country key="FR"></country>
</address>
</desc>
</hal:affiliation>
</affiliation>
</author>
<author><name sortKey="Morice, Christophe" sort="Morice, Christophe" uniqKey="Morice C" first="Christophe" last="Morice">Christophe Morice</name>
<affiliation><hal:affiliation type="institution" xml:id="struct-553076" status="INCOMING"> <orgName>Prestwick Chemical [Illkirch, Strasbourg]</orgName>
<desc> <address> <country key="FR"></country>
</address>
</desc>
</hal:affiliation>
</affiliation>
</author>
<author><name sortKey="Querat, Gilles" sort="Querat, Gilles" uniqKey="Querat G" first="Gilles" last="Quérat">Gilles Quérat</name>
<affiliation><hal:affiliation type="laboratory" xml:id="struct-86891" status="OLD"> <idno type="IdRef">124942504</idno>
<idno type="RNSR">200817677J</idno>
<orgName>Emergence des Pathologies Virales</orgName>
<orgName type="acronym">EPV</orgName>
<date type="start">2008</date>
<date type="end">2017-12-31</date>
<desc> <address> <addrLine>Faculté de médecine27 Bd jean Moulin13385 Marseille Cedex 5</addrLine>
<country key="FR"></country>
</address>
<ref type="url">https://www.ird.fr/la-recherche/liste-des-unites-de-recherche/190-emergence-des-pathologies-virales</ref>
</desc>
<listRelation> <relation name="UMR_D 190" active="#struct-303623" type="direct"></relation>
<relation name="UMR_D 190" active="#struct-67872" type="direct"></relation>
<relation name="UMR_D 190" active="#struct-198056" type="direct"></relation>
<relation name="UMR_D 190" active="#struct-300073" type="direct"></relation>
</listRelation>
<tutelles><tutelle name="UMR_D 190" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR_D 190" active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID"> <orgName>Institut de Recherche pour le Développement</orgName>
<orgName type="acronym">IRD</orgName>
<desc> <address> <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.ird.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR_D 190" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno>
<idno type="ISNI">0000 0001 2176 4817</idno>
<orgName>Aix Marseille Université</orgName>
<orgName type="acronym">AMU</orgName>
<date type="start">2012-01-01</date>
<desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-amu.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR_D 190" active="#struct-300073" type="direct"><org type="institution" xml:id="struct-300073" status="VALID"> <orgName>Assistance Publique - Hôpitaux de Marseille</orgName>
<orgName type="acronym">APHM</orgName>
<desc> <address> <addrLine>Direction générale AP-HM80, rue Brochier13 354 Marseille Cedex 5</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://fr.ap-hm.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author><name sortKey="Jung, Marie Louise" sort="Jung, Marie Louise" uniqKey="Jung M" first="Marie-Louise" last="Jung">Marie-Louise Jung</name>
<affiliation><hal:affiliation type="institution" xml:id="struct-553076" status="INCOMING"> <orgName>Prestwick Chemical [Illkirch, Strasbourg]</orgName>
<desc> <address> <country key="FR"></country>
</address>
</desc>
</hal:affiliation>
</affiliation>
</author>
<author><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name>
<affiliation><hal:affiliation type="laboratory" xml:id="struct-199349" status="OLD"> <idno type="IdRef">149981651</idno>
<idno type="RNSR">201220260Z</idno>
<orgName>Architecture et fonction des macromolécules biologiques</orgName>
<orgName type="acronym">AFMB</orgName>
<date type="start">2012-01-01</date>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.afmb.univ-mrs.fr/</ref>
</desc>
<listRelation> <relation name="UMR7257" active="#struct-92114" type="direct"></relation>
<relation name="UMR7257" active="#struct-198056" type="direct"></relation>
<relation name="UMR7257" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle name="UMR7257" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="OLD"> <orgName>Institut National de la Recherche Agronomique</orgName>
<orgName type="acronym">INRA</orgName>
<date type="start">1946-05-18</date>
<date type="end">2019-12-31</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.inra.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7257" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno>
<idno type="ISNI">0000 0001 2176 4817</idno>
<orgName>Aix Marseille Université</orgName>
<orgName type="acronym">AMU</orgName>
<date type="start">2012-01-01</date>
<desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-amu.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7257" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="ISNI">0000000122597504</idno>
<idno type="IdRef">02636817X</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author><name sortKey="Guillemot, Jean Claude" sort="Guillemot, Jean Claude" uniqKey="Guillemot J" first="Jean-Claude" last="Guillemot">Jean-Claude Guillemot</name>
<affiliation><hal:affiliation type="laboratory" xml:id="struct-199349" status="OLD"> <idno type="IdRef">149981651</idno>
<idno type="RNSR">201220260Z</idno>
<orgName>Architecture et fonction des macromolécules biologiques</orgName>
<orgName type="acronym">AFMB</orgName>
<date type="start">2012-01-01</date>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.afmb.univ-mrs.fr/</ref>
</desc>
<listRelation> <relation name="UMR7257" active="#struct-92114" type="direct"></relation>
<relation name="UMR7257" active="#struct-198056" type="direct"></relation>
<relation name="UMR7257" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle name="UMR7257" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="OLD"> <orgName>Institut National de la Recherche Agronomique</orgName>
<orgName type="acronym">INRA</orgName>
<date type="start">1946-05-18</date>
<date type="end">2019-12-31</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.inra.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7257" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno>
<idno type="ISNI">0000 0001 2176 4817</idno>
<orgName>Aix Marseille Université</orgName>
<orgName type="acronym">AMU</orgName>
<date type="start">2012-01-01</date>
<desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-amu.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7257" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="ISNI">0000000122597504</idno>
<idno type="IdRef">02636817X</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author><name sortKey="Decroly, Etienne" sort="Decroly, Etienne" uniqKey="Decroly E" first="Etienne" last="Decroly">Etienne Decroly</name>
<affiliation><hal:affiliation type="laboratory" xml:id="struct-199349" status="OLD"> <idno type="IdRef">149981651</idno>
<idno type="RNSR">201220260Z</idno>
<orgName>Architecture et fonction des macromolécules biologiques</orgName>
<orgName type="acronym">AFMB</orgName>
<date type="start">2012-01-01</date>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.afmb.univ-mrs.fr/</ref>
</desc>
<listRelation> <relation name="UMR7257" active="#struct-92114" type="direct"></relation>
<relation name="UMR7257" active="#struct-198056" type="direct"></relation>
<relation name="UMR7257" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle name="UMR7257" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="OLD"> <orgName>Institut National de la Recherche Agronomique</orgName>
<orgName type="acronym">INRA</orgName>
<date type="start">1946-05-18</date>
<date type="end">2019-12-31</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.inra.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7257" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno>
<idno type="ISNI">0000 0001 2176 4817</idno>
<orgName>Aix Marseille Université</orgName>
<orgName type="acronym">AMU</orgName>
<date type="start">2012-01-01</date>
<desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-amu.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7257" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="ISNI">0000000122597504</idno>
<idno type="IdRef">02636817X</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">HAL</idno>
<idno type="RBID">Hal:hal-02349530</idno>
<idno type="halId">hal-02349530</idno>
<idno type="halUri">https://hal-amu.archives-ouvertes.fr/hal-02349530</idno>
<idno type="url">https://hal-amu.archives-ouvertes.fr/hal-02349530</idno>
<idno type="doi">10.1016/j.antiviral.2017.06.021</idno>
<date when="2017-08">2017-08</date>
<idno type="wicri:Area/Hal/Corpus">000022</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Toward the identification of viral cap-methyltransferase inhibitors by fluorescence screening assay</title>
<author><name sortKey="Aouadi, Wahiba" sort="Aouadi, Wahiba" uniqKey="Aouadi W" first="Wahiba" last="Aouadi">Wahiba Aouadi</name>
<affiliation><hal:affiliation type="laboratory" xml:id="struct-199349" status="OLD"> <idno type="IdRef">149981651</idno>
<idno type="RNSR">201220260Z</idno>
<orgName>Architecture et fonction des macromolécules biologiques</orgName>
<orgName type="acronym">AFMB</orgName>
<date type="start">2012-01-01</date>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.afmb.univ-mrs.fr/</ref>
</desc>
<listRelation> <relation name="UMR7257" active="#struct-92114" type="direct"></relation>
<relation name="UMR7257" active="#struct-198056" type="direct"></relation>
<relation name="UMR7257" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle name="UMR7257" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="OLD"> <orgName>Institut National de la Recherche Agronomique</orgName>
<orgName type="acronym">INRA</orgName>
<date type="start">1946-05-18</date>
<date type="end">2019-12-31</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.inra.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7257" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno>
<idno type="ISNI">0000 0001 2176 4817</idno>
<orgName>Aix Marseille Université</orgName>
<orgName type="acronym">AMU</orgName>
<date type="start">2012-01-01</date>
<desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-amu.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7257" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="ISNI">0000000122597504</idno>
<idno type="IdRef">02636817X</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author><name sortKey="Ecilia Eydoux, C" sort="Ecilia Eydoux, C" uniqKey="Ecilia Eydoux C" first="C" last="Ecilia Eydoux">C. Ecilia Eydoux</name>
<affiliation><hal:affiliation type="laboratory" xml:id="struct-199349" status="OLD"> <idno type="IdRef">149981651</idno>
<idno type="RNSR">201220260Z</idno>
<orgName>Architecture et fonction des macromolécules biologiques</orgName>
<orgName type="acronym">AFMB</orgName>
<date type="start">2012-01-01</date>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.afmb.univ-mrs.fr/</ref>
</desc>
<listRelation> <relation name="UMR7257" active="#struct-92114" type="direct"></relation>
<relation name="UMR7257" active="#struct-198056" type="direct"></relation>
<relation name="UMR7257" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle name="UMR7257" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="OLD"> <orgName>Institut National de la Recherche Agronomique</orgName>
<orgName type="acronym">INRA</orgName>
<date type="start">1946-05-18</date>
<date type="end">2019-12-31</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.inra.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7257" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno>
<idno type="ISNI">0000 0001 2176 4817</idno>
<orgName>Aix Marseille Université</orgName>
<orgName type="acronym">AMU</orgName>
<date type="start">2012-01-01</date>
<desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-amu.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7257" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="ISNI">0000000122597504</idno>
<idno type="IdRef">02636817X</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author><name sortKey="Coutard, Bruno" sort="Coutard, Bruno" uniqKey="Coutard B" first="Bruno" last="Coutard">Bruno Coutard</name>
<affiliation><hal:affiliation type="laboratory" xml:id="struct-199349" status="OLD"> <idno type="IdRef">149981651</idno>
<idno type="RNSR">201220260Z</idno>
<orgName>Architecture et fonction des macromolécules biologiques</orgName>
<orgName type="acronym">AFMB</orgName>
<date type="start">2012-01-01</date>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.afmb.univ-mrs.fr/</ref>
</desc>
<listRelation> <relation name="UMR7257" active="#struct-92114" type="direct"></relation>
<relation name="UMR7257" active="#struct-198056" type="direct"></relation>
<relation name="UMR7257" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle name="UMR7257" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="OLD"> <orgName>Institut National de la Recherche Agronomique</orgName>
<orgName type="acronym">INRA</orgName>
<date type="start">1946-05-18</date>
<date type="end">2019-12-31</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.inra.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7257" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno>
<idno type="ISNI">0000 0001 2176 4817</idno>
<orgName>Aix Marseille Université</orgName>
<orgName type="acronym">AMU</orgName>
<date type="start">2012-01-01</date>
<desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-amu.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7257" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="ISNI">0000000122597504</idno>
<idno type="IdRef">02636817X</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author><name sortKey="Martin, Baptiste" sort="Martin, Baptiste" uniqKey="Martin B" first="Baptiste" last="Martin">Baptiste Martin</name>
<affiliation><hal:affiliation type="laboratory" xml:id="struct-199349" status="OLD"> <idno type="IdRef">149981651</idno>
<idno type="RNSR">201220260Z</idno>
<orgName>Architecture et fonction des macromolécules biologiques</orgName>
<orgName type="acronym">AFMB</orgName>
<date type="start">2012-01-01</date>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.afmb.univ-mrs.fr/</ref>
</desc>
<listRelation> <relation name="UMR7257" active="#struct-92114" type="direct"></relation>
<relation name="UMR7257" active="#struct-198056" type="direct"></relation>
<relation name="UMR7257" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle name="UMR7257" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="OLD"> <orgName>Institut National de la Recherche Agronomique</orgName>
<orgName type="acronym">INRA</orgName>
<date type="start">1946-05-18</date>
<date type="end">2019-12-31</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.inra.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7257" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno>
<idno type="ISNI">0000 0001 2176 4817</idno>
<orgName>Aix Marseille Université</orgName>
<orgName type="acronym">AMU</orgName>
<date type="start">2012-01-01</date>
<desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-amu.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7257" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="ISNI">0000000122597504</idno>
<idno type="IdRef">02636817X</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author><name sortKey="Debart, Francoise" sort="Debart, Francoise" uniqKey="Debart F" first="Françoise" last="Debart">Françoise Debart</name>
<affiliation><hal:affiliation type="laboratory" xml:id="struct-24488" status="VALID"> <idno type="RNSR">200711912X</idno>
<orgName>Institut des Biomolécules Max Mousseron [Pôle Chimie Balard]</orgName>
<orgName type="acronym">IBMM</orgName>
<desc> <address> <addrLine>Faculté de Pharmacie - 15 Av. Charles Flahault - BP 14 491 - 34093 Montpellier Cedex 5</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.ibmm.univ-montp1.fr</ref>
</desc>
<listRelation> <relation active="#struct-300370" type="direct"></relation>
<relation active="#struct-410122" type="direct"></relation>
<relation name="UMR5247" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-300370" type="direct"><org type="institution" xml:id="struct-300370" status="VALID"> <orgName>Ecole Nationale Supérieure de Chimie de Montpellier</orgName>
<orgName type="acronym">ENSCM</orgName>
<desc> <address> <addrLine>8 Rue de l'Ecole Normale 34296 Montpellier Cedex 5</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.enscm.fr</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-410122" type="direct"><org type="institution" xml:id="struct-410122" status="VALID"> <idno type="ISNI">0000000120970141</idno>
<orgName>Université de Montpellier</orgName>
<orgName type="acronym">UM</orgName>
<desc> <address> <addrLine>163 rue Auguste Broussonnet - 34090 Montpellier</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.umontpellier.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR5247" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="ISNI">0000000122597504</idno>
<idno type="IdRef">02636817X</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author><name sortKey="Jacques Vasseur, Jean" sort="Jacques Vasseur, Jean" uniqKey="Jacques Vasseur J" first="Jean" last="Jacques Vasseur">Jean Jacques Vasseur</name>
<affiliation><hal:affiliation type="laboratory" xml:id="struct-24488" status="VALID"> <idno type="RNSR">200711912X</idno>
<orgName>Institut des Biomolécules Max Mousseron [Pôle Chimie Balard]</orgName>
<orgName type="acronym">IBMM</orgName>
<desc> <address> <addrLine>Faculté de Pharmacie - 15 Av. Charles Flahault - BP 14 491 - 34093 Montpellier Cedex 5</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.ibmm.univ-montp1.fr</ref>
</desc>
<listRelation> <relation active="#struct-300370" type="direct"></relation>
<relation active="#struct-410122" type="direct"></relation>
<relation name="UMR5247" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-300370" type="direct"><org type="institution" xml:id="struct-300370" status="VALID"> <orgName>Ecole Nationale Supérieure de Chimie de Montpellier</orgName>
<orgName type="acronym">ENSCM</orgName>
<desc> <address> <addrLine>8 Rue de l'Ecole Normale 34296 Montpellier Cedex 5</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.enscm.fr</ref>
</desc>
</org>
</tutelle>
<tutelle active="#struct-410122" type="direct"><org type="institution" xml:id="struct-410122" status="VALID"> <idno type="ISNI">0000000120970141</idno>
<orgName>Université de Montpellier</orgName>
<orgName type="acronym">UM</orgName>
<desc> <address> <addrLine>163 rue Auguste Broussonnet - 34090 Montpellier</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.umontpellier.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR5247" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="ISNI">0000000122597504</idno>
<idno type="IdRef">02636817X</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author><name sortKey="Contreras, Jean" sort="Contreras, Jean" uniqKey="Contreras J" first="Jean" last="Contreras">Jean Contreras</name>
<affiliation><hal:affiliation type="institution" xml:id="struct-553076" status="INCOMING"> <orgName>Prestwick Chemical [Illkirch, Strasbourg]</orgName>
<desc> <address> <country key="FR"></country>
</address>
</desc>
</hal:affiliation>
</affiliation>
</author>
<author><name sortKey="Morice, Christophe" sort="Morice, Christophe" uniqKey="Morice C" first="Christophe" last="Morice">Christophe Morice</name>
<affiliation><hal:affiliation type="institution" xml:id="struct-553076" status="INCOMING"> <orgName>Prestwick Chemical [Illkirch, Strasbourg]</orgName>
<desc> <address> <country key="FR"></country>
</address>
</desc>
</hal:affiliation>
</affiliation>
</author>
<author><name sortKey="Querat, Gilles" sort="Querat, Gilles" uniqKey="Querat G" first="Gilles" last="Quérat">Gilles Quérat</name>
<affiliation><hal:affiliation type="laboratory" xml:id="struct-86891" status="OLD"> <idno type="IdRef">124942504</idno>
<idno type="RNSR">200817677J</idno>
<orgName>Emergence des Pathologies Virales</orgName>
<orgName type="acronym">EPV</orgName>
<date type="start">2008</date>
<date type="end">2017-12-31</date>
<desc> <address> <addrLine>Faculté de médecine27 Bd jean Moulin13385 Marseille Cedex 5</addrLine>
<country key="FR"></country>
</address>
<ref type="url">https://www.ird.fr/la-recherche/liste-des-unites-de-recherche/190-emergence-des-pathologies-virales</ref>
</desc>
<listRelation> <relation name="UMR_D 190" active="#struct-303623" type="direct"></relation>
<relation name="UMR_D 190" active="#struct-67872" type="direct"></relation>
<relation name="UMR_D 190" active="#struct-198056" type="direct"></relation>
<relation name="UMR_D 190" active="#struct-300073" type="direct"></relation>
</listRelation>
<tutelles><tutelle name="UMR_D 190" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
<orgName type="acronym">INSERM</orgName>
<desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.inserm.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR_D 190" active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID"> <orgName>Institut de Recherche pour le Développement</orgName>
<orgName type="acronym">IRD</orgName>
<desc> <address> <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.ird.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR_D 190" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno>
<idno type="ISNI">0000 0001 2176 4817</idno>
<orgName>Aix Marseille Université</orgName>
<orgName type="acronym">AMU</orgName>
<date type="start">2012-01-01</date>
<desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-amu.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR_D 190" active="#struct-300073" type="direct"><org type="institution" xml:id="struct-300073" status="VALID"> <orgName>Assistance Publique - Hôpitaux de Marseille</orgName>
<orgName type="acronym">APHM</orgName>
<desc> <address> <addrLine>Direction générale AP-HM80, rue Brochier13 354 Marseille Cedex 5</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://fr.ap-hm.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author><name sortKey="Jung, Marie Louise" sort="Jung, Marie Louise" uniqKey="Jung M" first="Marie-Louise" last="Jung">Marie-Louise Jung</name>
<affiliation><hal:affiliation type="institution" xml:id="struct-553076" status="INCOMING"> <orgName>Prestwick Chemical [Illkirch, Strasbourg]</orgName>
<desc> <address> <country key="FR"></country>
</address>
</desc>
</hal:affiliation>
</affiliation>
</author>
<author><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name>
<affiliation><hal:affiliation type="laboratory" xml:id="struct-199349" status="OLD"> <idno type="IdRef">149981651</idno>
<idno type="RNSR">201220260Z</idno>
<orgName>Architecture et fonction des macromolécules biologiques</orgName>
<orgName type="acronym">AFMB</orgName>
<date type="start">2012-01-01</date>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.afmb.univ-mrs.fr/</ref>
</desc>
<listRelation> <relation name="UMR7257" active="#struct-92114" type="direct"></relation>
<relation name="UMR7257" active="#struct-198056" type="direct"></relation>
<relation name="UMR7257" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle name="UMR7257" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="OLD"> <orgName>Institut National de la Recherche Agronomique</orgName>
<orgName type="acronym">INRA</orgName>
<date type="start">1946-05-18</date>
<date type="end">2019-12-31</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.inra.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7257" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno>
<idno type="ISNI">0000 0001 2176 4817</idno>
<orgName>Aix Marseille Université</orgName>
<orgName type="acronym">AMU</orgName>
<date type="start">2012-01-01</date>
<desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-amu.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7257" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="ISNI">0000000122597504</idno>
<idno type="IdRef">02636817X</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author><name sortKey="Guillemot, Jean Claude" sort="Guillemot, Jean Claude" uniqKey="Guillemot J" first="Jean-Claude" last="Guillemot">Jean-Claude Guillemot</name>
<affiliation><hal:affiliation type="laboratory" xml:id="struct-199349" status="OLD"> <idno type="IdRef">149981651</idno>
<idno type="RNSR">201220260Z</idno>
<orgName>Architecture et fonction des macromolécules biologiques</orgName>
<orgName type="acronym">AFMB</orgName>
<date type="start">2012-01-01</date>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.afmb.univ-mrs.fr/</ref>
</desc>
<listRelation> <relation name="UMR7257" active="#struct-92114" type="direct"></relation>
<relation name="UMR7257" active="#struct-198056" type="direct"></relation>
<relation name="UMR7257" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle name="UMR7257" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="OLD"> <orgName>Institut National de la Recherche Agronomique</orgName>
<orgName type="acronym">INRA</orgName>
<date type="start">1946-05-18</date>
<date type="end">2019-12-31</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.inra.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7257" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno>
<idno type="ISNI">0000 0001 2176 4817</idno>
<orgName>Aix Marseille Université</orgName>
<orgName type="acronym">AMU</orgName>
<date type="start">2012-01-01</date>
<desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-amu.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7257" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="ISNI">0000000122597504</idno>
<idno type="IdRef">02636817X</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
<author><name sortKey="Decroly, Etienne" sort="Decroly, Etienne" uniqKey="Decroly E" first="Etienne" last="Decroly">Etienne Decroly</name>
<affiliation><hal:affiliation type="laboratory" xml:id="struct-199349" status="OLD"> <idno type="IdRef">149981651</idno>
<idno type="RNSR">201220260Z</idno>
<orgName>Architecture et fonction des macromolécules biologiques</orgName>
<orgName type="acronym">AFMB</orgName>
<date type="start">2012-01-01</date>
<date type="end">2019-12-31</date>
<desc> <address> <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.afmb.univ-mrs.fr/</ref>
</desc>
<listRelation> <relation name="UMR7257" active="#struct-92114" type="direct"></relation>
<relation name="UMR7257" active="#struct-198056" type="direct"></relation>
<relation name="UMR7257" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle name="UMR7257" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="OLD"> <orgName>Institut National de la Recherche Agronomique</orgName>
<orgName type="acronym">INRA</orgName>
<date type="start">1946-05-18</date>
<date type="end">2019-12-31</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.inra.fr</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7257" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno>
<idno type="ISNI">0000 0001 2176 4817</idno>
<orgName>Aix Marseille Université</orgName>
<orgName type="acronym">AMU</orgName>
<date type="start">2012-01-01</date>
<desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-amu.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7257" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="ISNI">0000000122597504</idno>
<idno type="IdRef">02636817X</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc> <address> <country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
</affiliation>
</author>
</analytic>
<idno type="DOI">10.1016/j.antiviral.2017.06.021</idno>
<series><title level="j">Antiviral Research</title>
<idno type="ISSN">0166-3542</idno>
<imprint><date type="datePub">2017-08</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="mix" xml:lang="en"><term>Antiviral</term>
<term>Coronavirus</term>
<term>Flavivirus</term>
<term>HTRF</term>
<term>Inhibitor</term>
<term>Methyltransferase</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"> <p>Two highly pathogenic human coronaviruses associated with severe respiratory syndromes emerged since the beginning of the century. The severe acute respiratory syndrome SARS-coronavirus (CoV) spread first in southern China in 2003 with about 8000 infected cases in few months. Then in 2012, the Middle East respiratory syndrome (MERS-CoV) emerged from the Arabian Peninsula giving a still ongoing epidemic associated to a high fatality rate. CoVs are thus considered a major health threat. This is especially true as no vaccine nor specific therapeutic are available against either SARS-or MERS-CoV. Therefore, new drugs need to be identified in order to develop antiviral treatments limiting CoV repli-cation. In this study, we focus on the nsp14 protein, which plays a key role in virus replication as it methylates the RNA cap structure at the N7 position of the guanine. We developed a high-throughput N7-MTase assay based on Homogenous Time Resolved Fluorescence (HTRF ®) and screened chemical libraries (2000 compounds) on the SARS-CoV nsp14. 20 compounds inhibiting the SARS-CoV nsp14 were further evaluated by IC 50 determination and their specificity was assessed toward flavivirus-and human cap N7-MTases. Our results reveal three classes of compounds: 1) molecules inhibiting several MTases as well as the dengue virus polymerase activity unspecifically, 2) pan MTases inhibitors targeting both viral and cellular MTases, and 3) inhibitors targeting one viral MTase more specifically showing however activity against the human cap N7-MTase. These compounds provide a first basis towards the development of more specific inhibitors of viral methyltransferases.</p>
</div>
</front>
</TEI>
<hal api="V3"> <titleStmt> <title xml:lang="en">Toward the identification of viral cap-methyltransferase inhibitors by fluorescence screening assay</title>
<author role="aut"> <persName> <forename type="first">Wahiba</forename>
<surname>Aouadi</surname>
</persName>
<idno type="halauthorid">11210181</idno>
<affiliation ref="#struct-199349"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">C</forename>
<surname>Ecilia Eydoux</surname>
</persName>
<idno type="halauthorid">11637336</idno>
<affiliation ref="#struct-199349"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Bruno</forename>
<surname>Coutard</surname>
</persName>
<email type="md5">47692dfde289f361565df138bc756d88</email>
<email type="domain">afmb.univ-mrs.fr</email>
<idno type="halauthorid">728888</idno>
<affiliation ref="#struct-199349"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Baptiste</forename>
<surname>Martin</surname>
</persName>
<idno type="halauthorid">1458382</idno>
<affiliation ref="#struct-199349"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Françoise</forename>
<surname>Debart</surname>
</persName>
<idno type="halauthorid">90202</idno>
<affiliation ref="#struct-24488"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Jean</forename>
<surname>Jacques Vasseur</surname>
</persName>
<idno type="halauthorid">11637337</idno>
<affiliation ref="#struct-24488"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Jean</forename>
<forename type="middle">Marie</forename>
<surname>Contreras</surname>
</persName>
<idno type="halauthorid">11637338</idno>
<affiliation ref="#struct-553076"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Christophe</forename>
<surname>Morice</surname>
</persName>
<idno type="halauthorid">11210324</idno>
<affiliation ref="#struct-553076"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Gilles</forename>
<surname>Quérat</surname>
</persName>
<idno type="halauthorid">11210325</idno>
<affiliation ref="#struct-86891"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Marie-Louise</forename>
<surname>Jung</surname>
</persName>
<idno type="halauthorid">11210326</idno>
<affiliation ref="#struct-553076"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Bruno</forename>
<surname>Canard</surname>
</persName>
<email type="md5">b082b648c100da933978ce65df2a1ad4</email>
<email type="domain">afmb.univ-mrs.fr</email>
<idno type="halauthorid">728889</idno>
<affiliation ref="#struct-199349"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Jean-Claude</forename>
<surname>Guillemot</surname>
</persName>
<idno type="halauthorid">119833</idno>
<affiliation ref="#struct-199349"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Etienne</forename>
<surname>Decroly</surname>
</persName>
<email type="md5">5d4b15d0e684f3ec48f16b6e5eaefe49</email>
<email type="domain">afmb.univ-mrs.fr</email>
<idno type="idhal" notation="string">etienne-decroly</idno>
<idno type="idhal" notation="numeric">178424</idno>
<idno type="halauthorid">726902</idno>
<idno type="ORCID">https://orcid.org/0000-0002-6046-024X</idno>
<affiliation ref="#struct-199349"></affiliation>
</author>
<editor role="depositor"> <persName> <forename>Etienne</forename>
<surname>Decroly</surname>
</persName>
<email type="md5">5d4b15d0e684f3ec48f16b6e5eaefe49</email>
<email type="domain">afmb.univ-mrs.fr</email>
</editor>
</titleStmt>
<editionStmt> <edition n="v1" type="current"> <date type="whenSubmitted">2019-11-05 16:57:37</date>
<date type="whenModified">2020-03-17 02:54:55</date>
<date type="whenReleased">2019-11-06 08:19:04</date>
<date type="whenProduced">2017-08</date>
<date type="whenEndEmbargoed">2019-11-05</date>
<ref type="file" target="https://hal-amu.archives-ouvertes.fr/hal-02349530/document"> <date notBefore="2019-11-05"></date>
</ref>
<ref type="file" subtype="greenPublisher" n="1" target="https://hal-amu.archives-ouvertes.fr/hal-02349530/file/1-s2.0-S0166354217303911-main.pdf"> <date notBefore="2019-11-05"></date>
</ref>
<ref type="externalLink" target="https://hal-amu.archives-ouvertes.fr/hal-02349530/file/1-s2.0-S0166354217303911-main.pdf"></ref>
</edition>
<respStmt> <resp>contributor</resp>
<name key="467958"> <persName> <forename>Etienne</forename>
<surname>Decroly</surname>
</persName>
<email type="md5">5d4b15d0e684f3ec48f16b6e5eaefe49</email>
<email type="domain">afmb.univ-mrs.fr</email>
</name>
</respStmt>
</editionStmt>
<publicationStmt> <distributor>CCSD</distributor>
<idno type="halId">hal-02349530</idno>
<idno type="halUri">https://hal-amu.archives-ouvertes.fr/hal-02349530</idno>
<idno type="halBibtex">aouadi:hal-02349530</idno>
<idno type="halRefHtml">Antiviral Research, Elsevier Masson, 2017, 144, pp.330-339. ⟨10.1016/j.antiviral.2017.06.021⟩</idno>
<idno type="halRef">Antiviral Research, Elsevier Masson, 2017, 144, pp.330-339. ⟨10.1016/j.antiviral.2017.06.021⟩</idno>
</publicationStmt>
<seriesStmt> <idno type="stamp" n="UNIV-AMU">Aix Marseille Université</idno>
<idno type="stamp" n="ENSC-MONTPELLIER">Ecole Nationale Supérieure de Chimie de Montpellier</idno>
<idno type="stamp" n="INRA">INRA - Institut national de la recherche agronomique</idno>
<idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
<idno type="stamp" n="IBMM">Institut des Biomolécules Max Mousseron</idno>
<idno type="stamp" n="AGREENIUM">Archive ouverte en agrobiosciences</idno>
<idno type="stamp" n="IRD">IRD - Institut de recherche pour le développement</idno>
<idno type="stamp" n="INC-CNRS">Institut de Chimie du CNRS</idno>
<idno type="stamp" n="CHIMIE">Chimie</idno>
<idno type="stamp" n="UNIV-MONTPELLIER">Université de Montpellier</idno>
<idno type="stamp" n="TEST-AMU">Espace de test AMU</idno>
<idno type="stamp" n="INRAE">Institut National de Recherche en Agriculture, Alimentation et Environnement</idno>
</seriesStmt>
<notesStmt> <note type="audience" n="2">International</note>
<note type="popular" n="0">No</note>
<note type="peer" n="1">Yes</note>
</notesStmt>
<sourceDesc> <biblStruct> <analytic> <title xml:lang="en">Toward the identification of viral cap-methyltransferase inhibitors by fluorescence screening assay</title>
<author role="aut"> <persName> <forename type="first">Wahiba</forename>
<surname>Aouadi</surname>
</persName>
<idno type="halauthorid">11210181</idno>
<affiliation ref="#struct-199349"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">C</forename>
<surname>Ecilia Eydoux</surname>
</persName>
<idno type="halauthorid">11637336</idno>
<affiliation ref="#struct-199349"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Bruno</forename>
<surname>Coutard</surname>
</persName>
<email type="md5">47692dfde289f361565df138bc756d88</email>
<email type="domain">afmb.univ-mrs.fr</email>
<idno type="halauthorid">728888</idno>
<affiliation ref="#struct-199349"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Baptiste</forename>
<surname>Martin</surname>
</persName>
<idno type="halauthorid">1458382</idno>
<affiliation ref="#struct-199349"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Françoise</forename>
<surname>Debart</surname>
</persName>
<idno type="halauthorid">90202</idno>
<affiliation ref="#struct-24488"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Jean</forename>
<surname>Jacques Vasseur</surname>
</persName>
<idno type="halauthorid">11637337</idno>
<affiliation ref="#struct-24488"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Jean</forename>
<forename type="middle">Marie</forename>
<surname>Contreras</surname>
</persName>
<idno type="halauthorid">11637338</idno>
<affiliation ref="#struct-553076"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Christophe</forename>
<surname>Morice</surname>
</persName>
<idno type="halauthorid">11210324</idno>
<affiliation ref="#struct-553076"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Gilles</forename>
<surname>Quérat</surname>
</persName>
<idno type="halauthorid">11210325</idno>
<affiliation ref="#struct-86891"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Marie-Louise</forename>
<surname>Jung</surname>
</persName>
<idno type="halauthorid">11210326</idno>
<affiliation ref="#struct-553076"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Bruno</forename>
<surname>Canard</surname>
</persName>
<email type="md5">b082b648c100da933978ce65df2a1ad4</email>
<email type="domain">afmb.univ-mrs.fr</email>
<idno type="halauthorid">728889</idno>
<affiliation ref="#struct-199349"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Jean-Claude</forename>
<surname>Guillemot</surname>
</persName>
<idno type="halauthorid">119833</idno>
<affiliation ref="#struct-199349"></affiliation>
</author>
<author role="aut"> <persName> <forename type="first">Etienne</forename>
<surname>Decroly</surname>
</persName>
<email type="md5">5d4b15d0e684f3ec48f16b6e5eaefe49</email>
<email type="domain">afmb.univ-mrs.fr</email>
<idno type="idhal" notation="string">etienne-decroly</idno>
<idno type="idhal" notation="numeric">178424</idno>
<idno type="halauthorid">726902</idno>
<idno type="ORCID">https://orcid.org/0000-0002-6046-024X</idno>
<affiliation ref="#struct-199349"></affiliation>
</author>
</analytic>
<monogr> <idno type="halJournalId" status="VALID">10572</idno>
<idno type="issn">0166-3542</idno>
<title level="j">Antiviral Research</title>
<imprint> <publisher>Elsevier Masson</publisher>
<biblScope unit="volume">144</biblScope>
<biblScope unit="pp">330-339</biblScope>
<date type="datePub">2017-08</date>
</imprint>
</monogr>
<idno type="doi">10.1016/j.antiviral.2017.06.021</idno>
</biblStruct>
</sourceDesc>
<profileDesc> <langUsage> <language ident="en">English</language>
</langUsage>
<textClass> <keywords scheme="author"> <term xml:lang="en">Flavivirus</term>
<term xml:lang="en">Inhibitor</term>
<term xml:lang="en">Coronavirus</term>
<term xml:lang="en">Methyltransferase</term>
<term xml:lang="en">HTRF</term>
<term xml:lang="en">Antiviral</term>
</keywords>
<classCode scheme="halDomain" n="sdv">Life Sciences [q-bio]</classCode>
<classCode scheme="halDomain" n="sdv.bbm">Life Sciences [q-bio]/Biochemistry, Molecular Biology</classCode>
<classCode scheme="halDomain" n="sdv.bbm.bc">Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]</classCode>
<classCode scheme="halTypology" n="ART">Journal articles</classCode>
</textClass>
<abstract xml:lang="en"> <p>Two highly pathogenic human coronaviruses associated with severe respiratory syndromes emerged since the beginning of the century. The severe acute respiratory syndrome SARS-coronavirus (CoV) spread first in southern China in 2003 with about 8000 infected cases in few months. Then in 2012, the Middle East respiratory syndrome (MERS-CoV) emerged from the Arabian Peninsula giving a still ongoing epidemic associated to a high fatality rate. CoVs are thus considered a major health threat. This is especially true as no vaccine nor specific therapeutic are available against either SARS-or MERS-CoV. Therefore, new drugs need to be identified in order to develop antiviral treatments limiting CoV repli-cation. In this study, we focus on the nsp14 protein, which plays a key role in virus replication as it methylates the RNA cap structure at the N7 position of the guanine. We developed a high-throughput N7-MTase assay based on Homogenous Time Resolved Fluorescence (HTRF ®) and screened chemical libraries (2000 compounds) on the SARS-CoV nsp14. 20 compounds inhibiting the SARS-CoV nsp14 were further evaluated by IC 50 determination and their specificity was assessed toward flavivirus-and human cap N7-MTases. Our results reveal three classes of compounds: 1) molecules inhibiting several MTases as well as the dengue virus polymerase activity unspecifically, 2) pan MTases inhibitors targeting both viral and cellular MTases, and 3) inhibitors targeting one viral MTase more specifically showing however activity against the human cap N7-MTase. These compounds provide a first basis towards the development of more specific inhibitors of viral methyltransferases.</p>
</abstract>
</profileDesc>
</hal>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Hal/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000022 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Hal/Corpus/biblio.hfd -nk 000022 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Hal |étape= Corpus |type= RBID |clé= Hal:hal-02349530 |texte= Toward the identification of viral cap-methyltransferase inhibitors by fluorescence screening assay }}
![]() | This area was generated with Dilib version V0.6.33. | ![]() |