Use of specific oligonucleotides for direct enumeration of Listeria monocytogenes in food samples by colony hybridization and rapid detection by PCR.
Identifieur interne : 000434 ( France/Analysis ); précédent : 000433; suivant : 000435Use of specific oligonucleotides for direct enumeration of Listeria monocytogenes in food samples by colony hybridization and rapid detection by PCR.
Auteurs : M. Bohnert [France] ; F. Dilasser ; C. Dalet ; J. Mengaud ; P. CossartSource :
- Research in microbiology [ 0923-2508 ]
Descripteurs français
- KwdFr :
- MESH :
- génétique : ADN bactérien, Listeria monocytogenes, Oligonucléotides.
- isolement et purification : Listeria monocytogenes.
- physiologie : Hybridation d'acides nucléiques.
- Microbiologie alimentaire, Réaction de polymérisation en chaîne, Techniques in vitro.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : DNA, Bacterial, Oligonucleotides.
- genetics : Listeria monocytogenes.
- isolation & purification : Listeria monocytogenes.
- methods : Polymerase Chain Reaction.
- physiology : Nucleic Acid Hybridization.
- Food Microbiology, In Vitro Techniques.
Abstract
Two 18-mer oligonucleotides derived from the sequence of hly, the gene coding for listeriolysin O, were shown to be specific for Listeria monocytogenes in the genus Listeria in colony hybridization tests. The oligonucleotides did not hybridize with any of the bacterial species found in food and co-isolated with Listeria on selective media. They were used in colony hybridization tests for enumeration of L. monocytogenes present in food samples after direct plating on selective media plates. In addition, two 24-mer oligonucleotides, each including the sequence of one of the 18-mers, were successfully used for the PCR-based detection of L. monocytogenes bacilli present in food samples after 48-h enrichment period. Using this technique, as little as 10(2) bacteria per ml of enrichment broth can be detected.
DOI: 10.1016/0923-2508(92)90019-k
PubMed: 1448613
Affiliations:
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pubmed:1448613Le document en format XML
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Bohnert</name><affiliation wicri:level="3"><nlm:affiliation>LCHA-CNEVA, Paris.</nlm:affiliation><country>France</country><placeName><settlement type="city">Paris</settlement><region type="région" nuts="2">Île-de-France</region></placeName><wicri:orgArea>LCHA-CNEVA</wicri:orgArea></affiliation></author><author><name sortKey="Dilasser, F" sort="Dilasser, F" uniqKey="Dilasser F" first="F" last="Dilasser">F. Dilasser</name></author><author><name sortKey="Dalet, C" sort="Dalet, C" uniqKey="Dalet C" first="C" last="Dalet">C. Dalet</name></author><author><name sortKey="Mengaud, J" sort="Mengaud, J" uniqKey="Mengaud J" first="J" last="Mengaud">J. Mengaud</name></author><author><name sortKey="Cossart, P" sort="Cossart, P" uniqKey="Cossart P" first="P" last="Cossart">P. Cossart</name></author></analytic><series><title level="j">Research in microbiology</title><idno type="ISSN">0923-2508</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA, Bacterial (genetics)</term><term>Food Microbiology</term><term>In Vitro Techniques</term><term>Listeria monocytogenes (genetics)</term><term>Listeria monocytogenes (isolation & purification)</term><term>Nucleic Acid Hybridization (physiology)</term><term>Oligonucleotides (genetics)</term><term>Polymerase Chain Reaction (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (génétique)</term><term>Hybridation d'acides nucléiques (physiologie)</term><term>Listeria monocytogenes (génétique)</term><term>Listeria monocytogenes (isolement et purification)</term><term>Microbiologie alimentaire</term><term>Oligonucléotides (génétique)</term><term>Réaction de polymérisation en chaîne ()</term><term>Techniques in vitro</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Bacterial</term><term>Oligonucleotides</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Listeria monocytogenes</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN bactérien</term><term>Listeria monocytogenes</term><term>Oligonucléotides</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Listeria monocytogenes</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Listeria monocytogenes</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Hybridation d'acides nucléiques</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Nucleic Acid Hybridization</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Food Microbiology</term><term>In Vitro Techniques</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Microbiologie alimentaire</term><term>Réaction de polymérisation en chaîne</term><term>Techniques in vitro</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Two 18-mer oligonucleotides derived from the sequence of hly, the gene coding for listeriolysin O, were shown to be specific for Listeria monocytogenes in the genus Listeria in colony hybridization tests. The oligonucleotides did not hybridize with any of the bacterial species found in food and co-isolated with Listeria on selective media. They were used in colony hybridization tests for enumeration of L. monocytogenes present in food samples after direct plating on selective media plates. In addition, two 24-mer oligonucleotides, each including the sequence of one of the 18-mers, were successfully used for the PCR-based detection of L. monocytogenes bacilli present in food samples after 48-h enrichment period. Using this technique, as little as 10(2) bacteria per ml of enrichment broth can be detected.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Île-de-France</li></region><settlement><li>Paris</li></settlement></list><tree><noCountry><name sortKey="Cossart, P" sort="Cossart, P" uniqKey="Cossart P" first="P" last="Cossart">P. Cossart</name><name sortKey="Dalet, C" sort="Dalet, C" uniqKey="Dalet C" first="C" last="Dalet">C. Dalet</name><name sortKey="Dilasser, F" sort="Dilasser, F" uniqKey="Dilasser F" first="F" last="Dilasser">F. Dilasser</name><name sortKey="Mengaud, J" sort="Mengaud, J" uniqKey="Mengaud J" first="J" last="Mengaud">J. Mengaud</name></noCountry><country name="France"><region name="Île-de-France"><name sortKey="Bohnert, M" sort="Bohnert, M" uniqKey="Bohnert M" first="M" last="Bohnert">M. Bohnert</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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