Transcription of the alpha globin gene domain in normal and AEV-transformed chicken erythroblasts: Mapping of giant globin-specific RNA including embryonic and adult genes
Identifieur interne : 000416 ( France/Analysis ); précédent : 000415; suivant : 000417Transcription of the alpha globin gene domain in normal and AEV-transformed chicken erythroblasts: Mapping of giant globin-specific RNA including embryonic and adult genes
Auteurs : F. Broders [France] ; K. Scherrer [France]Source :
- Molecular and General Genetics MGG [ 0026-8925 ] ; 1987-09-01.
English descriptors
- KwdEn :
- Teeft :
- Acad, Adult chicken, Adult genes, Agarose, Alpha, Alpha globin gene domain, Atrls, Avian, Avian erythroblasts, Biol, Broders, Cdna, Cdna probes, Denaturing, Denaturing conditions, Dnase, Dodgson, Domain, Embryonic, Engel, Erythroblast, Faint signals, First exon, Fragment, Full domain, Gene, Gene screen, Genomic, Globin, Globin domain, Globin gene, Globin gene domain, Globin gene transcripts, Globin genes, Globin mrna, Hind, Homogeneous bands, Hybridization, Hybridized, Linkers, Matrix, Molecular weight, Molecular weight markers, Moreau, Mrna, Natl, Nick translation, Normal cell, Normal cells, Nuclear matrix, Nucleic acids, Phage, Plasmid, Polymerase, Primary transcript, Primer, Primer extension experiments, Probe, Proc, Proc natl acad, Razin, Recombinant plasmids, Repetitive, Repetitive sequences, Reynaud, Room temperature, Scherrer, Size determination, Subcloned, Therwath, Transcript, Transcription, Transcriptional domain, Weintraub.
Abstract
Summary: The genomic domain of about 20 kbp of the chicken alpha-type globin genes, framed by AT-rich linkers (ATRLs; Moreau et al. 1982) and repetitive sequences (Broders et al. 1986), was cut into 13 fragments and subcloned. The in vitro labelled individual restriction fragments were used to test the extent of the transcribed domain by blot-hybridization of nuclear RNA in large excess from normal adult chicken and Avian Erythroblastosis Virus (AEV)-transformed erythroblasts. In both these types of cells, the AT-rich segments situated 6 kbp upstream of the first gene as well as all the domain including the embryonic pi and the adult alpha D and alpha A genes down to the AT-rich segment placed 3 kbp downstream were found to be transcribed. Electrophoresis of nuclear RNA, Northern blotting and hybridization with most of the nick-translated DNA probes revealed in all cases the presence of heterogeneous globin RNA molecules in the 3–13 kb range, as well as some distinct RNA bands. Single-stranded RNA probes of some genomic segments indicated asymmetrical transcription of the minus strand. A 12 kb globin-specific RNA including the pi and alpha A genes but not the intervening alpha D gene was observed in AEV-transformed cells: it includes sequences located far upstream and downstream from the alpha globin genes and might represent a processing product of a full length transcript spanning the whole domain. Reverse transcription by extension of primers placed in the first exon of each of the three globin genes confirmed the presence of continuous transcripts of the domain including the two adult and the embryonic globin genes.
Url:
DOI: 10.1007/BF00329645
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001264
- to stream Istex, to step Curation: 001264
- to stream Istex, to step Checkpoint: 002192
- to stream Main, to step Merge: 004D92
- to stream Main, to step Curation: 004D12
- to stream Main, to step Exploration: 004D12
- to stream France, to step Extraction: 000416
Links to Exploration step
ISTEX:E97FC4D683A75DD59C1EC83C01470F306B7AC7ADLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Transcription of the alpha globin gene domain in normal and AEV-transformed chicken erythroblasts: Mapping of giant globin-specific RNA including embryonic and adult genes</title><author><name sortKey="Broders, F" sort="Broders, F" uniqKey="Broders F" first="F." last="Broders">F. Broders</name></author><author><name sortKey="Scherrer, K" sort="Scherrer, K" uniqKey="Scherrer K" first="K." last="Scherrer">K. Scherrer</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:E97FC4D683A75DD59C1EC83C01470F306B7AC7AD</idno><date when="1987" year="1987">1987</date><idno type="doi">10.1007/BF00329645</idno><idno type="url">https://api.istex.fr/ark:/67375/1BB-97LDQQLX-3/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001264</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001264</idno><idno type="wicri:Area/Istex/Curation">001264</idno><idno type="wicri:Area/Istex/Checkpoint">002192</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">002192</idno><idno type="wicri:doubleKey">0026-8925:1987:Broders F:transcription:of:the</idno><idno type="wicri:Area/Main/Merge">004D92</idno><idno type="wicri:Area/Main/Curation">004D12</idno><idno type="wicri:Area/Main/Exploration">004D12</idno><idno type="wicri:Area/France/Extraction">000416</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Transcription of the alpha globin gene domain in normal and AEV-transformed chicken erythroblasts: Mapping of giant globin-specific RNA including embryonic and adult genes</title><author><name sortKey="Broders, F" sort="Broders, F" uniqKey="Broders F" first="F." last="Broders">F. Broders</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Institut Jacques Monod, Université Paris VII, 2 Place Jussieu-Tour 43, F-75251, Paris Cedex 05</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Scherrer, K" sort="Scherrer, K" uniqKey="Scherrer K" first="K." last="Scherrer">K. Scherrer</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Institut Jacques Monod, Université Paris VII, 2 Place Jussieu-Tour 43, F-75251, Paris Cedex 05</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Molecular and General Genetics MGG</title><title level="j" type="abbrev">Molec Gen Genet</title><idno type="ISSN">0026-8925</idno><idno type="eISSN">1432-1874</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1987-09-01">1987-09-01</date><biblScope unit="volume">209</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="210">210</biblScope><biblScope unit="page" to="220">220</biblScope></imprint><idno type="ISSN">0026-8925</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0026-8925</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>AEV</term><term>AT-rich linkers</term><term>Globin genes</term><term>Oncogenes</term><term>Pre-mRNA</term><term>Repetitive DNA</term><term>Transcription</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Acad</term><term>Adult chicken</term><term>Adult genes</term><term>Agarose</term><term>Alpha</term><term>Alpha globin gene domain</term><term>Atrls</term><term>Avian</term><term>Avian erythroblasts</term><term>Biol</term><term>Broders</term><term>Cdna</term><term>Cdna probes</term><term>Denaturing</term><term>Denaturing conditions</term><term>Dnase</term><term>Dodgson</term><term>Domain</term><term>Embryonic</term><term>Engel</term><term>Erythroblast</term><term>Faint signals</term><term>First exon</term><term>Fragment</term><term>Full domain</term><term>Gene</term><term>Gene screen</term><term>Genomic</term><term>Globin</term><term>Globin domain</term><term>Globin gene</term><term>Globin gene domain</term><term>Globin gene transcripts</term><term>Globin genes</term><term>Globin mrna</term><term>Hind</term><term>Homogeneous bands</term><term>Hybridization</term><term>Hybridized</term><term>Linkers</term><term>Matrix</term><term>Molecular weight</term><term>Molecular weight markers</term><term>Moreau</term><term>Mrna</term><term>Natl</term><term>Nick translation</term><term>Normal cell</term><term>Normal cells</term><term>Nuclear matrix</term><term>Nucleic acids</term><term>Phage</term><term>Plasmid</term><term>Polymerase</term><term>Primary transcript</term><term>Primer</term><term>Primer extension experiments</term><term>Probe</term><term>Proc</term><term>Proc natl acad</term><term>Razin</term><term>Recombinant plasmids</term><term>Repetitive</term><term>Repetitive sequences</term><term>Reynaud</term><term>Room temperature</term><term>Scherrer</term><term>Size determination</term><term>Subcloned</term><term>Therwath</term><term>Transcript</term><term>Transcription</term><term>Transcriptional domain</term><term>Weintraub</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: The genomic domain of about 20 kbp of the chicken alpha-type globin genes, framed by AT-rich linkers (ATRLs; Moreau et al. 1982) and repetitive sequences (Broders et al. 1986), was cut into 13 fragments and subcloned. The in vitro labelled individual restriction fragments were used to test the extent of the transcribed domain by blot-hybridization of nuclear RNA in large excess from normal adult chicken and Avian Erythroblastosis Virus (AEV)-transformed erythroblasts. In both these types of cells, the AT-rich segments situated 6 kbp upstream of the first gene as well as all the domain including the embryonic pi and the adult alpha D and alpha A genes down to the AT-rich segment placed 3 kbp downstream were found to be transcribed. Electrophoresis of nuclear RNA, Northern blotting and hybridization with most of the nick-translated DNA probes revealed in all cases the presence of heterogeneous globin RNA molecules in the 3–13 kb range, as well as some distinct RNA bands. Single-stranded RNA probes of some genomic segments indicated asymmetrical transcription of the minus strand. A 12 kb globin-specific RNA including the pi and alpha A genes but not the intervening alpha D gene was observed in AEV-transformed cells: it includes sequences located far upstream and downstream from the alpha globin genes and might represent a processing product of a full length transcript spanning the whole domain. Reverse transcription by extension of primers placed in the first exon of each of the three globin genes confirmed the presence of continuous transcripts of the domain including the two adult and the embryonic globin genes.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Île-de-France</li></region><settlement><li>Paris</li></settlement></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Broders, F" sort="Broders, F" uniqKey="Broders F" first="F." last="Broders">F. Broders</name></region><name sortKey="Scherrer, K" sort="Scherrer, K" uniqKey="Scherrer K" first="K." last="Scherrer">K. Scherrer</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/France/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000416 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000416 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= France
|étape= Analysis
|type= RBID
|clé= ISTEX:E97FC4D683A75DD59C1EC83C01470F306B7AC7AD
|texte= Transcription of the alpha globin gene domain in normal and AEV-transformed chicken erythroblasts: Mapping of giant globin-specific RNA including embryonic and adult genes
}}
| This area was generated with Dilib version V0.6.33. |  |