Difference in the expression of asymmetric acetylcholinesterase molecular forms during myogenesis in early avian dermomyotomes and limb buds in ovo and in vitro
Identifieur interne : 000413 ( France/Analysis ); précédent : 000412; suivant : 000414Difference in the expression of asymmetric acetylcholinesterase molecular forms during myogenesis in early avian dermomyotomes and limb buds in ovo and in vitro
Auteurs : F. M. Vallette [France] ; M. Fauquet [France] ; M. A. Teillet [France]Source :
- Developmental Biology [ 0012-1606 ] ; 1987.
English descriptors
- Teeft :
- Academic press, Acetylcholinesterase, Ache, Ache activity, Activity peaks, Asymmetric, Asymmetric forms, Biol, Biosynthesis, Cellular extracts, Chick, Chick dermomyotomes, Chick embryo, Chick embryos, Crest cells, Culture medium, Dermomyotomal, Dermomyotomal cells, Dermomyotome, Dermomyotome explants, Dermomyotomes, Developmental biology, Different sets, Douarin, Early stages, Embryo, Embryonic development, Explants, Fauquet, Globular, Globular forms, Hauschka, Limb, Limb buds, Molecular forms, Mononucleated, Muscular differentiation, Myoblasts, Myogenesis, Myogenic, Myogenic cells, Myotubes, Neural, Neural crest, Neural induction, Neural influence, Neural tube, Quail, Quail dermomyotomes, Quail embryo, Quail embryo dermomyotomes, Quail embryos, Sedimentation profiles, Somite, Total ache activity, Toutant, Vallette, Vigny.
Abstract
Abstract: The A12 (asymmetric) form of acetylcholinesterase (AChE) is generally considered to be synthesized in leg muscle tissues by myotubes under neural influence, but not by myoblasts. We have examined the expression of the different molecular forms of AChE in explants of developing limb buds and dermomyotomes (the myogenic part of the somites) obtained from 3-day-old chick and quail embryos, either directly after removal or during in vitro culture. We describe a muscular differentiation of both territories in vitro, leading to the formation of myotubes which are morphologically similar to the class of early muscle cells described by Bonner and Hauschka (1974). In vivo the A12 form is present in quail dermomyotomes which are almost entirely composed of mononucleated poorly differentiated cells; in contrast, it is absent from similar cells in chick dermomyotomes and from limb buds in both species. This shows that in the case of quail embryos the appearance of the A12 form precedes the fusion of myoblasts into myotubes. In both species, dermomyotome explants express asymmetric and globular forms of the enzyme during muscular differentiation in vitro, whereas limb buds synthesize only globular forms. After surgical removal of neural tube and/or neural crest at 2 days in ovo, the biosynthesis of the A forms in quail dermomyotomes is not suppressed and is consequently not dependent upon prior connection of the dermomyotomes to central neurons or upon the presence of autonomic precursors. Since limb bud muscle cells derive from somites our results raise questions concerning the differentiation of migrating somitic cells in this territory where a neural influence appears necessary to induce the biosynthesis of asymmetric AChE forms.
Url:
DOI: 10.1016/0012-1606(87)90105-9
Affiliations:
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M." last="Vallette">F. M. Vallette</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Neurobiologie, Ecole Normale Supérieure, 46 rue d'Ulm, 75005 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Fauquet, M" sort="Fauquet, M" uniqKey="Fauquet M" first="M." last="Fauquet">M. Fauquet</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Institut d'Embryologie du CNRS et du Collège de France, 49bis Avenue de la Belle Gabrielle, 94130 Nogent sur Marne</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Nogent-sur-Marne</settlement></placeName></affiliation></author><author><name sortKey="Teillet, M A" sort="Teillet, M A" uniqKey="Teillet M" first="M. A." last="Teillet">M. A. Teillet</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Institut d'Embryologie du CNRS et du Collège de France, 49bis Avenue de la Belle Gabrielle, 94130 Nogent sur Marne</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Nogent-sur-Marne</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Developmental Biology</title><title level="j" type="abbrev">YDBIO</title><idno type="ISSN">0012-1606</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1987">1987</date><biblScope unit="volume">120</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="77">77</biblScope><biblScope unit="page" to="84">84</biblScope></imprint><idno type="ISSN">0012-1606</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0012-1606</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Academic press</term><term>Acetylcholinesterase</term><term>Ache</term><term>Ache activity</term><term>Activity peaks</term><term>Asymmetric</term><term>Asymmetric forms</term><term>Biol</term><term>Biosynthesis</term><term>Cellular extracts</term><term>Chick</term><term>Chick dermomyotomes</term><term>Chick embryo</term><term>Chick embryos</term><term>Crest cells</term><term>Culture medium</term><term>Dermomyotomal</term><term>Dermomyotomal cells</term><term>Dermomyotome</term><term>Dermomyotome explants</term><term>Dermomyotomes</term><term>Developmental biology</term><term>Different sets</term><term>Douarin</term><term>Early stages</term><term>Embryo</term><term>Embryonic development</term><term>Explants</term><term>Fauquet</term><term>Globular</term><term>Globular forms</term><term>Hauschka</term><term>Limb</term><term>Limb buds</term><term>Molecular forms</term><term>Mononucleated</term><term>Muscular differentiation</term><term>Myoblasts</term><term>Myogenesis</term><term>Myogenic</term><term>Myogenic cells</term><term>Myotubes</term><term>Neural</term><term>Neural crest</term><term>Neural induction</term><term>Neural influence</term><term>Neural tube</term><term>Quail</term><term>Quail dermomyotomes</term><term>Quail embryo</term><term>Quail embryo dermomyotomes</term><term>Quail embryos</term><term>Sedimentation profiles</term><term>Somite</term><term>Total ache activity</term><term>Toutant</term><term>Vallette</term><term>Vigny</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The A12 (asymmetric) form of acetylcholinesterase (AChE) is generally considered to be synthesized in leg muscle tissues by myotubes under neural influence, but not by myoblasts. We have examined the expression of the different molecular forms of AChE in explants of developing limb buds and dermomyotomes (the myogenic part of the somites) obtained from 3-day-old chick and quail embryos, either directly after removal or during in vitro culture. We describe a muscular differentiation of both territories in vitro, leading to the formation of myotubes which are morphologically similar to the class of early muscle cells described by Bonner and Hauschka (1974). In vivo the A12 form is present in quail dermomyotomes which are almost entirely composed of mononucleated poorly differentiated cells; in contrast, it is absent from similar cells in chick dermomyotomes and from limb buds in both species. This shows that in the case of quail embryos the appearance of the A12 form precedes the fusion of myoblasts into myotubes. In both species, dermomyotome explants express asymmetric and globular forms of the enzyme during muscular differentiation in vitro, whereas limb buds synthesize only globular forms. After surgical removal of neural tube and/or neural crest at 2 days in ovo, the biosynthesis of the A forms in quail dermomyotomes is not suppressed and is consequently not dependent upon prior connection of the dermomyotomes to central neurons or upon the presence of autonomic precursors. Since limb bud muscle cells derive from somites our results raise questions concerning the differentiation of migrating somitic cells in this territory where a neural influence appears necessary to induce the biosynthesis of asymmetric AChE forms.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Île-de-France</li></region><settlement><li>Nogent-sur-Marne</li><li>Paris</li></settlement></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Vallette, F M" sort="Vallette, F M" uniqKey="Vallette F" first="F. M." last="Vallette">F. M. Vallette</name></region><name sortKey="Fauquet, M" sort="Fauquet, M" uniqKey="Fauquet M" first="M." last="Fauquet">M. Fauquet</name><name sortKey="Teillet, M A" sort="Teillet, M A" uniqKey="Teillet M" first="M. A." last="Teillet">M. A. Teillet</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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