Interferon-induced early changes in nuclear protein interactions with the interferon consensus sequence.
Identifieur interne : 000396 ( France/Analysis ); précédent : 000395; suivant : 000397Interferon-induced early changes in nuclear protein interactions with the interferon consensus sequence.
Auteurs : C. Roy [France] ; B. LebleuSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 1989.
Descripteurs français
- KwdFr :
- Cellules HeLa, Deoxyribonuclease I (pharmacologie), Interférons (pharmacologie), Masse moléculaire, Noyau de la cellule (métabolisme), Oligodésoxyribonucléotides (métabolisme), Protéines de liaison à l'ADN (métabolisme), Protéines nucléaires (métabolisme), Séquences d'acides nucléiques régulatrices, Techniques in vitro.
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : DNA-Binding Proteins, Nuclear Proteins, Oligodeoxyribonucleotides.
- metabolism : Cell Nucleus.
- chemical , pharmacology : Deoxyribonuclease I, Interferons.
- HeLa Cells, In Vitro Techniques, Molecular Weight, Regulatory Sequences, Nucleic Acid.
Abstract
A number of genes are transcriptionally regulated by interferons via a cis acting regulatory element (interferon consensus sequence). After incubation with HeLa cell nuclear extracts, a synthetic 29 mers probe representing the consensus was retarded as a single band whose amount increased after the addition of IFN to the cells. DNase I footprinting showed that a region of the non coding strand was protected differentially upon interferon treatment. After nuclear protein blotting and probing with the oligonucleotide, two proteins (114 and 50 kDa) of the desired specificity were revealed. Their absolute and relative amounts do not appear to be dependent of the IFN treatment.
DOI: 10.1016/0006-291x(89)92145-1
PubMed: 2476126
Affiliations:
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pubmed:2476126Le document en format XML
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Roy</name><affiliation wicri:level="1"><nlm:affiliation>U.A. CNRS 1191, Laboratoire de Biochimie des Protéines, Université de Montpellier II, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>U.A. CNRS 1191, Laboratoire de Biochimie des Protéines, Université de Montpellier II</wicri:regionArea><wicri:noRegion>Université de Montpellier II</wicri:noRegion><wicri:noRegion>Université de Montpellier II</wicri:noRegion></affiliation></author><author><name sortKey="Lebleu, B" sort="Lebleu, B" uniqKey="Lebleu B" first="B" last="Lebleu">B. Lebleu</name></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="ISSN">0006-291X</idno><imprint><date when="1989" type="published">1989</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Nucleus (metabolism)</term><term>DNA-Binding Proteins (metabolism)</term><term>Deoxyribonuclease I (pharmacology)</term><term>HeLa Cells</term><term>In Vitro Techniques</term><term>Interferons (pharmacology)</term><term>Molecular Weight</term><term>Nuclear Proteins (metabolism)</term><term>Oligodeoxyribonucleotides (metabolism)</term><term>Regulatory Sequences, Nucleic Acid</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cellules HeLa</term><term>Deoxyribonuclease I (pharmacologie)</term><term>Interférons (pharmacologie)</term><term>Masse moléculaire</term><term>Noyau de la cellule (métabolisme)</term><term>Oligodésoxyribonucléotides (métabolisme)</term><term>Protéines de liaison à l'ADN (métabolisme)</term><term>Protéines nucléaires (métabolisme)</term><term>Séquences d'acides nucléiques régulatrices</term><term>Techniques in vitro</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA-Binding Proteins</term><term>Nuclear Proteins</term><term>Oligodeoxyribonucleotides</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Nucleus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Noyau de la cellule</term><term>Oligodésoxyribonucléotides</term><term>Protéines de liaison à l'ADN</term><term>Protéines nucléaires</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Deoxyribonuclease I</term><term>Interférons</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Deoxyribonuclease I</term><term>Interferons</term></keywords><keywords scheme="MESH" xml:lang="en"><term>HeLa Cells</term><term>In Vitro Techniques</term><term>Molecular Weight</term><term>Regulatory Sequences, Nucleic Acid</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cellules HeLa</term><term>Masse moléculaire</term><term>Séquences d'acides nucléiques régulatrices</term><term>Techniques in vitro</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A number of genes are transcriptionally regulated by interferons via a cis acting regulatory element (interferon consensus sequence). After incubation with HeLa cell nuclear extracts, a synthetic 29 mers probe representing the consensus was retarded as a single band whose amount increased after the addition of IFN to the cells. DNase I footprinting showed that a region of the non coding strand was protected differentially upon interferon treatment. After nuclear protein blotting and probing with the oligonucleotide, two proteins (114 and 50 kDa) of the desired specificity were revealed. Their absolute and relative amounts do not appear to be dependent of the IFN treatment.</div></front></TEI><affiliations><list><country><li>France</li></country></list><tree><noCountry><name sortKey="Lebleu, B" sort="Lebleu, B" uniqKey="Lebleu B" first="B" last="Lebleu">B. Lebleu</name></noCountry><country name="France"><noRegion><name sortKey="Roy, C" sort="Roy, C" uniqKey="Roy C" first="C" last="Roy">C. Roy</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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