Characterization of the 5′ and promoter regions of the gene encoding the mouse neuronal cell adhesion molecule F3
Identifieur interne : 000326 ( France/Analysis ); précédent : 000325; suivant : 000327Characterization of the 5′ and promoter regions of the gene encoding the mouse neuronal cell adhesion molecule F3
Auteurs : Maura Buttiglione [Italie] ; Giuseppina Cangiano [Italie] ; Christo Goridis [France] ; Gianfranco Gennarini [Italie, France]Source :
- Molecular Brain Research [ 0169-328X ] ; 1995.
English descriptors
- Teeft :
- Adhesion, Adhesion molecules, Amplification, Amplification products, Antisense oligonucleotide, Binding site, Binding sites, Binding transcription factors, Biol, Buttiglione, Cdna, Cdna products, Cell adhesion, Cell line, Chain promoter, Clone, Consensus sequence, Correct orientation, Corresponding sequences, Developmental regulation, Different domains, Embo, Encoding, Enhancer, Enhancer element, Exon, Febs lett, Fisiologia umana, Forebrain, Gene, Gene encoding, Gene expression, Gene organization, Gene transcription, Gennarini, Genomic, Genomic clones encoding, Genomic region, Glycoprotein, Goridis, Granule cells, Hincli fragments, Immunoglobulin, Immunoglobulin superfamily, Molecular brain research, Molecule, Morphoregulatory molecules, Mouse forebrain, Mouse genomic library, Mouse glycoprotein gene, Ncam, Ncam promoters, Negative results, Nervous tissue, Neural, Neural cell adhesion molecule, Neural cell adhesion molecule gene, Neural morphogenesis, Neurite, Neurite extension, Neurite growth, Neurite outgrowth, Neuroblastoma cells, Neuron, Neuronal, Neuronal cell adhesion molecule, Neuronal cells, Neurosci, Nucleotide, Oligonucleotides, Pblcat3 vector, Pcat control vector, Primer, Promoter, Promoter activity, Promoter region, Promoter regions, Promoter sequences, Protection experiments, Reporter gene expression, Rnaase protection, Rougon, Signal peptide, Single site, Specific expression, Sralpha promoter, Structural analysis, Structural characterization, Transcription, Transcription factor, Transcription factor binding, Transcription factors, Transcription initiation, Transcriptional activation, Transfection, Transfection efficiency, Untranslated region, Vector polylinker.
Abstract
Abstract: F3 is a 135 kDa neuronal cell surface adhesive glycoprotein belonging to the immunoglobulin supergene family (IgSF) which mediates heterophilic contact formation among neural cells and is involved in the control of neurite growth. F3 expression is regulated, during critical developmental periods, on neuronal subpopulations thus suggesting that control of F3 gene expression could be of morphogenetic relevance. To shed light on the mechanism involved in the control of F3 gene expression we isolated clones covering about 50 kilobases of the F3 gene which also included the promoter region. The study of F3 gene exon/intron organization revealed that, like other neural IgSF molecules, each of the first two F3 C2 domains is encoded by two exons while the N-terminus, the signal peptide and the 5′ untranslated region are each encoded by distinct exons. A single transcription start site was identified, surrounded by a short 114 by sequence able to direct reporter gene expression in both F3-expressing and -non-expressing cells. In addition, a cell type-specific enhancer, only active in F3-expressing cells, was found immediately upstream to it. Structural analysis of the promoter region revealed consensus sequences for binding transcription factors involved in cell type-specific and/or developmental regulations. Most of them are homeobox containing transcription factors thus suggesting that regulation of F3 gene expression could be part of a large developmental program.
Url:
DOI: 10.1016/0169-328X(94)00262-D
Affiliations:
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ISTEX:E22ECD77A16AC49349D43E6CC4A6526299E1A262Le document en format XML
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F-13288 Marseille Cedex 9</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Molecular Brain Research</title><title level="j" type="abbrev">BRESM</title><idno type="ISSN">0169-328X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1995">1995</date><biblScope unit="volume">29</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="297">297</biblScope><biblScope unit="page" to="309">309</biblScope></imprint><idno type="ISSN">0169-328X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0169-328X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Adhesion</term><term>Adhesion molecules</term><term>Amplification</term><term>Amplification products</term><term>Antisense oligonucleotide</term><term>Binding site</term><term>Binding sites</term><term>Binding transcription factors</term><term>Biol</term><term>Buttiglione</term><term>Cdna</term><term>Cdna products</term><term>Cell adhesion</term><term>Cell line</term><term>Chain promoter</term><term>Clone</term><term>Consensus sequence</term><term>Correct orientation</term><term>Corresponding sequences</term><term>Developmental regulation</term><term>Different domains</term><term>Embo</term><term>Encoding</term><term>Enhancer</term><term>Enhancer element</term><term>Exon</term><term>Febs lett</term><term>Fisiologia umana</term><term>Forebrain</term><term>Gene</term><term>Gene encoding</term><term>Gene expression</term><term>Gene organization</term><term>Gene transcription</term><term>Gennarini</term><term>Genomic</term><term>Genomic clones encoding</term><term>Genomic region</term><term>Glycoprotein</term><term>Goridis</term><term>Granule cells</term><term>Hincli fragments</term><term>Immunoglobulin</term><term>Immunoglobulin superfamily</term><term>Molecular brain research</term><term>Molecule</term><term>Morphoregulatory molecules</term><term>Mouse forebrain</term><term>Mouse genomic library</term><term>Mouse glycoprotein gene</term><term>Ncam</term><term>Ncam promoters</term><term>Negative results</term><term>Nervous tissue</term><term>Neural</term><term>Neural cell adhesion molecule</term><term>Neural cell adhesion molecule gene</term><term>Neural morphogenesis</term><term>Neurite</term><term>Neurite extension</term><term>Neurite growth</term><term>Neurite outgrowth</term><term>Neuroblastoma cells</term><term>Neuron</term><term>Neuronal</term><term>Neuronal cell adhesion molecule</term><term>Neuronal cells</term><term>Neurosci</term><term>Nucleotide</term><term>Oligonucleotides</term><term>Pblcat3 vector</term><term>Pcat control vector</term><term>Primer</term><term>Promoter</term><term>Promoter activity</term><term>Promoter region</term><term>Promoter regions</term><term>Promoter sequences</term><term>Protection experiments</term><term>Reporter gene expression</term><term>Rnaase protection</term><term>Rougon</term><term>Signal peptide</term><term>Single site</term><term>Specific expression</term><term>Sralpha promoter</term><term>Structural analysis</term><term>Structural characterization</term><term>Transcription</term><term>Transcription factor</term><term>Transcription factor binding</term><term>Transcription factors</term><term>Transcription initiation</term><term>Transcriptional activation</term><term>Transfection</term><term>Transfection efficiency</term><term>Untranslated region</term><term>Vector polylinker</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: F3 is a 135 kDa neuronal cell surface adhesive glycoprotein belonging to the immunoglobulin supergene family (IgSF) which mediates heterophilic contact formation among neural cells and is involved in the control of neurite growth. F3 expression is regulated, during critical developmental periods, on neuronal subpopulations thus suggesting that control of F3 gene expression could be of morphogenetic relevance. To shed light on the mechanism involved in the control of F3 gene expression we isolated clones covering about 50 kilobases of the F3 gene which also included the promoter region. The study of F3 gene exon/intron organization revealed that, like other neural IgSF molecules, each of the first two F3 C2 domains is encoded by two exons while the N-terminus, the signal peptide and the 5′ untranslated region are each encoded by distinct exons. A single transcription start site was identified, surrounded by a short 114 by sequence able to direct reporter gene expression in both F3-expressing and -non-expressing cells. In addition, a cell type-specific enhancer, only active in F3-expressing cells, was found immediately upstream to it. Structural analysis of the promoter region revealed consensus sequences for binding transcription factors involved in cell type-specific and/or developmental regulations. Most of them are homeobox containing transcription factors thus suggesting that regulation of F3 gene expression could be part of a large developmental program.</div></front></TEI><affiliations><list><country><li>France</li><li>Italie</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Marseille</li></settlement></list><tree><country name="Italie"><noRegion><name sortKey="Buttiglione, Maura" sort="Buttiglione, Maura" uniqKey="Buttiglione M" first="Maura" last="Buttiglione">Maura Buttiglione</name></noRegion><name sortKey="Cangiano, Giuseppina" sort="Cangiano, Giuseppina" uniqKey="Cangiano G" first="Giuseppina" last="Cangiano">Giuseppina Cangiano</name><name sortKey="Gennarini, Gianfranco" sort="Gennarini, Gianfranco" uniqKey="Gennarini G" first="Gianfranco" last="Gennarini">Gianfranco Gennarini</name></country><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Goridis, Christo" sort="Goridis, Christo" uniqKey="Goridis C" first="Christo" last="Goridis">Christo Goridis</name></region><name sortKey="Gennarini, Gianfranco" sort="Gennarini, Gianfranco" uniqKey="Gennarini G" first="Gianfranco" last="Gennarini">Gianfranco Gennarini</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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