MersV1, France, Analysis, bibRecord, 000313

The RU5 (‘R’) region from human leukaemia viruses (HTLV-1) contains an internal ribosome entry site (IRES)-like sequence

Identifieur interne : 000313 ( France/Analysis ); précédent : 000312; suivant : 000314

The RU5 (‘R’) region from human leukaemia viruses (HTLV-1) contains an internal ribosome entry site (IRES)-like sequence

Auteurs : Joé Attal [France] ; Marie-Claire Théron [France] ; Frédérique Taboit [France] ; Marco Cajero-Juarez [France] ; Guy Kann [France] ; Philippe Bolifraud [France] ; Louis Marie Houdebine [France]

Source :

FEBS Letters [ 0014-5793 ] ; 1996.

RBID : ISTEX:9685A7B64F3FD1304586E40CC3D558D52956EAF5

English descriptors

Teeft :
- Attal, Bicistronic, Bicistronic mrna, Bovine leukaemia virus, Cell leukaemia virus, Cell type, Cistron, Early gene, Edna, Edna poly, First cistron, Gene promoter, Hairpin loop, Internal ribosome entry site, Ire, Ires activity, Ires effect, Late genes, Leukaemia, Luciferase, Luciferase activity, Luciferase gene, Moloney mouse leukaemia virus, Mrna, Northern blot analysis, Picornavirus, Plasmid, Poliovirus, Poliovirus ires, Previous studies, Promoter, Ribosome, Second cistron, Transfected, Unpublished results.

Abstract

Abstract: RNA fragments containing the complete R region and the beginning of the U5 region (‘R’) from the human T cell leukaemia virus 1 (HTLV-1) stimulated the translation of the second cistrons in bicistronic mRNAs. The 5′ untranslated region from SV40 early genes (SU) which was unable to stimulate translation of second cistrons amplified markedly the internal ribosome entry site (IRES) effect of the HTLV-1 ‘R’ fragments. The ‘R’ regions from HTLV-1 have therefore properties similar to internal ribosome entry sites (IRES) originally found in picornavirus. The beginning of the U5 region from HTLV-1 contains a polypyrimidine sequence which is known to play an essential role in the IRES activity in picornavirus. The same experiments carried out using the ‘R’ region from bovine leukaemia virus (BLV) showed that this sequence has at most a weak IRES effect. One retroviruses HTLV-1 and perhaps others contain therefore an IRES activity. Interestingly, the combined SU ‘R’ sequence worked efficiently with different cistrons, different promoters and in all tested cell lines, whereas the poliovirus IRES was active in CHO cells but not in the mouse mammary cell line HC11. The SU ‘R’ sequence may therefore preferably be used to generate active bicistronic mRNAs.

Url:

https://api.istex.fr/ark:/67375/6H6-7J37WXZP-T/fulltext.pdf

DOI: 10.1016/0014-5793(96)00815-0

Affiliations:

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ISTEX:9685A7B64F3FD1304586E40CC3D558D52956EAF5

Le document en format XML

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<front><div type="abstract" xml:lang="en">Abstract: RNA fragments containing the complete R region and the beginning of the U5 region (‘R’) from the human T cell leukaemia virus 1 (HTLV-1) stimulated the translation of the second cistrons in bicistronic mRNAs. The 5′ untranslated region from SV40 early genes (SU) which was unable to stimulate translation of second cistrons amplified markedly the internal ribosome entry site (IRES) effect of the HTLV-1 ‘R’ fragments. The ‘R’ regions from HTLV-1 have therefore properties similar to internal ribosome entry sites (IRES) originally found in picornavirus. The beginning of the U5 region from HTLV-1 contains a polypyrimidine sequence which is known to play an essential role in the IRES activity in picornavirus. The same experiments carried out using the ‘R’ region from bovine leukaemia virus (BLV) showed that this sequence has at most a weak IRES effect. One retroviruses HTLV-1 and perhaps others contain therefore an IRES activity. Interestingly, the combined SU ‘R’ sequence worked efficiently with different cistrons, different promoters and in all tested cell lines, whereas the poliovirus IRES was active in CHO cells but not in the mouse mammary cell line HC11. The SU ‘R’ sequence may therefore preferably be used to generate active bicistronic mRNAs.</div>
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The RU5 (‘R’) region from human leukaemia viruses (HTLV-1) contains an internal ribosome entry site (IRES)-like sequence

The RU5 (‘R’) region from human leukaemia viruses (HTLV-1) contains an internal ribosome entry site (IRES)-like sequence

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

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