Optimization of RNA isolation from Brittle Leaf Disease affected date palm leaves and construction of a subtractive cDNA library.
Identifieur interne : 000761 ( PubMed/Corpus ); précédent : 000760; suivant : 000762Optimization of RNA isolation from Brittle Leaf Disease affected date palm leaves and construction of a subtractive cDNA library.
Auteurs : Mohammed Najib Saïdi ; Radhia Gargouri-Bouzid ; Mariem Rayanni ; Noureddine DriraSource :
- Molecular biotechnology [ 1073-6085 ] ; 2009.
English descriptors
- KwdEn :
- Arecaceae (genetics), Flavonoids (metabolism), Gene Expression Regulation, Plant (MeSH), Gene Library (MeSH), Mercaptoethanol (chemistry), Nucleic Acid Hybridization (MeSH), Octoxynol (MeSH), Phenols (metabolism), Plant Diseases (genetics), Plant Leaves (genetics), Polyethylene Glycols (chemistry), Polyphenols (MeSH), Povidone (chemistry), RNA, Plant (isolation & purification), RNA, Plant (metabolism).
- MESH :
- chemical , chemistry : Mercaptoethanol, Polyethylene Glycols, Povidone.
- chemical , isolation & purification : RNA, Plant.
- chemical , metabolism : Flavonoids, Phenols, RNA, Plant.
- genetics : Arecaceae, Plant Diseases, Plant Leaves.
- Gene Expression Regulation, Plant, Gene Library, Nucleic Acid Hybridization, Octoxynol, Polyphenols.
Abstract
A simple and efficient method was described here for the isolation of high-quality RNA from date palm leaves affected with Brittle Leaf Disease (BLD) and containing high amount of phenolic compounds. The procedure was based on the use of a non-ionic detergent Nonidet-P40 (NP-40), Polyvinylpyrrolidone (PVP), and beta-mercaptoethanol in the extraction buffer in order to isolate cytoplasmic RNA and to prevent the oxidation of phenolic compounds. This method allowed the isolation of intact RNA, suitable for cDNA synthesis and library construction. Differential screening of the subtractive cDNA library from affected leaf RNA led to the identification of some BLD-induced genes.
DOI: 10.1007/s12033-008-9104-1
PubMed: 18815905
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pubmed:18815905Le document en format XML
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The procedure was based on the use of a non-ionic detergent Nonidet-P40 (NP-40), Polyvinylpyrrolidone (PVP), and beta-mercaptoethanol in the extraction buffer in order to isolate cytoplasmic RNA and to prevent the oxidation of phenolic compounds. This method allowed the isolation of intact RNA, suitable for cDNA synthesis and library construction. Differential screening of the subtractive cDNA library from affected leaf RNA led to the identification of some BLD-induced genes.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18815905</PMID><DateCompleted><Year>2009</Year><Month>03</Month><Day>03</Day></DateCompleted><DateRevised><Year>2020</Year><Month>12</Month><Day>09</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1073-6085</ISSN><JournalIssue CitedMedium="Print"><Volume>41</Volume><Issue>1</Issue><PubDate><Year>2009</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Molecular biotechnology</Title><ISOAbbreviation>Mol Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Optimization of RNA isolation from Brittle Leaf Disease affected date palm leaves and construction of a subtractive cDNA library.</ArticleTitle><Pagination><MedlinePgn>63-8</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s12033-008-9104-1</ELocationID><Abstract><AbstractText>A simple and efficient method was described here for the isolation of high-quality RNA from date palm leaves affected with Brittle Leaf Disease (BLD) and containing high amount of phenolic compounds. The procedure was based on the use of a non-ionic detergent Nonidet-P40 (NP-40), Polyvinylpyrrolidone (PVP), and beta-mercaptoethanol in the extraction buffer in order to isolate cytoplasmic RNA and to prevent the oxidation of phenolic compounds. This method allowed the isolation of intact RNA, suitable for cDNA synthesis and library construction. Differential screening of the subtractive cDNA library from affected leaf RNA led to the identification of some BLD-induced genes.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Saïdi</LastName><ForeName>Mohammed Najib</ForeName><Initials>MN</Initials><AffiliationInfo><Affiliation>Laboratoire des Biotechnologies Végétales Appliquées à l'Amélioration des Cultures, Ecole Nationale d'Ingénieurs de Sfax, Route Soukra Km 4, Sfax, Tunisia. saidimn@yahoo.fr</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gargouri-Bouzid</LastName><ForeName>Radhia</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Rayanni</LastName><ForeName>Mariem</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Drira</LastName><ForeName>Noureddine</ForeName><Initials>N</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType 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Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015723" MajorTopicYN="Y">Gene Library</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008623" MajorTopicYN="N">Mercaptoethanol</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009693" MajorTopicYN="N">Nucleic Acid Hybridization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017830" MajorTopicYN="N">Octoxynol</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010636" MajorTopicYN="N">Phenols</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName 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