Two new gene clusters involved in the degradation of plant cell wall from the fecal microbiota of Tunisian dromedary.
Identifieur interne : 000360 ( PubMed/Corpus ); précédent : 000359; suivant : 000361Two new gene clusters involved in the degradation of plant cell wall from the fecal microbiota of Tunisian dromedary.
Auteurs : Rihab Ameri ; Elisabeth Laville ; Gabrielle Potocki-Véronèse ; Sahar Trabelsi ; Monia Mezghani ; Fatma Elgharbi ; Samir BejarSource :
- PloS one [ 1932-6203 ] ; 2018.
English descriptors
- KwdEn :
- Animals (MeSH), Camelus (microbiology), Cell Wall (metabolism), Cell Wall (microbiology), Cellulose (metabolism), Feces (microbiology), Microbiota (genetics), Molecular Sequence Annotation (MeSH), Multigene Family (genetics), Plant Cells (metabolism), Polysaccharides (metabolism), Sequence Analysis (MeSH).
- MESH :
- chemical , metabolism : Cellulose, Polysaccharides.
- genetics : Microbiota, Multigene Family.
- metabolism : Cell Wall, Plant Cells.
- microbiology : Camelus, Cell Wall, Feces.
- Animals, Molecular Sequence Annotation, Sequence Analysis.
Abstract
Dromedaries are capable of digesting plant cell wall with high content of lignocellulose of poor digestibility. Consequently, their intestinal microbiota can be a source of novel carbohydrate-active enzymes (CAZymes). To the best of our knowledge, no data are available describing the biochemical analysis of enzymes in dromedary intestinal microbiota. To investigate new hydrolytic enzymes from the dromedary gut, a fosmid library was constructed using metagenomic DNA from feces of non-domestic adult dromedary camels living in the Tunisian desert. High-throughput functional screening of 13756 clones resulted in 47 hit clones active on a panel of various chromogenic and non-chromogenic glycan substrates. Two of them, harboring multiple activities, were retained for further analysis. Clone 26H3 displayed activity on AZO-CM-cellulose, AZCL Carob galactomannan and Tween 20, while clone 36A23 was active on AZCL carob galactomannan and AZCL barley β-glucan. The functional annotation of their sequences highlighted original metagenomic loci originating from bacteria of the Bacteroidetes/Chlorobi group, involved in the metabolization of mannosides and β-glucans thanks to a complete battery of endo- and exo-acting glycoside hydrolases, esterases, phosphorylases and transporters.
DOI: 10.1371/journal.pone.0194621
PubMed: 29601586
PubMed Central: PMC5877837
Links to Exploration step
pubmed:29601586Le document en format XML
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last="Potocki-Véronèse">Gabrielle Potocki-Véronèse</name><affiliation><nlm:affiliation>LISBP, CNRS, INRA, INSAT, Université de Toulouse, Toulouse, France.</nlm:affiliation></affiliation></author><author><name sortKey="Trabelsi, Sahar" sort="Trabelsi, Sahar" uniqKey="Trabelsi S" first="Sahar" last="Trabelsi">Sahar Trabelsi</name><affiliation><nlm:affiliation>Laboratoire de Biotechnologie Microbienne et d'Ingénierie des Enzymes (LBMIE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Sfax, Tunisia.</nlm:affiliation></affiliation></author><author><name sortKey="Mezghani, Monia" sort="Mezghani, Monia" uniqKey="Mezghani M" first="Monia" last="Mezghani">Monia Mezghani</name><affiliation><nlm:affiliation>Laboratoire de Biotechnologie Microbienne et d'Ingénierie des Enzymes (LBMIE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Sfax, Tunisia.</nlm:affiliation></affiliation></author><author><name sortKey="Elgharbi, Fatma" sort="Elgharbi, Fatma" uniqKey="Elgharbi F" first="Fatma" last="Elgharbi">Fatma Elgharbi</name><affiliation><nlm:affiliation>Laboratoire de Biotechnologie Microbienne et d'Ingénierie des Enzymes (LBMIE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Sfax, Tunisia.</nlm:affiliation></affiliation></author><author><name sortKey="Bejar, Samir" sort="Bejar, Samir" uniqKey="Bejar S" first="Samir" last="Bejar">Samir Bejar</name><affiliation><nlm:affiliation>Laboratoire de Biotechnologie Microbienne et d'Ingénierie des Enzymes (LBMIE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Sfax, Tunisia.</nlm:affiliation></affiliation></author></analytic><series><title level="j">PloS one</title><idno type="eISSN">1932-6203</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Camelus (microbiology)</term><term>Cell Wall (metabolism)</term><term>Cell Wall (microbiology)</term><term>Cellulose (metabolism)</term><term>Feces (microbiology)</term><term>Microbiota (genetics)</term><term>Molecular Sequence Annotation (MeSH)</term><term>Multigene Family (genetics)</term><term>Plant Cells (metabolism)</term><term>Polysaccharides (metabolism)</term><term>Sequence Analysis (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cellulose</term><term>Polysaccharides</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Microbiota</term><term>Multigene Family</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term><term>Plant Cells</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Camelus</term><term>Cell Wall</term><term>Feces</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Molecular Sequence Annotation</term><term>Sequence Analysis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Dromedaries are capable of digesting plant cell wall with high content of lignocellulose of poor digestibility. Consequently, their intestinal microbiota can be a source of novel carbohydrate-active enzymes (CAZymes). To the best of our knowledge, no data are available describing the biochemical analysis of enzymes in dromedary intestinal microbiota. To investigate new hydrolytic enzymes from the dromedary gut, a fosmid library was constructed using metagenomic DNA from feces of non-domestic adult dromedary camels living in the Tunisian desert. High-throughput functional screening of 13756 clones resulted in 47 hit clones active on a panel of various chromogenic and non-chromogenic glycan substrates. Two of them, harboring multiple activities, were retained for further analysis. Clone 26H3 displayed activity on AZO-CM-cellulose, AZCL Carob galactomannan and Tween 20, while clone 36A23 was active on AZCL carob galactomannan and AZCL barley β-glucan. The functional annotation of their sequences highlighted original metagenomic loci originating from bacteria of the Bacteroidetes/Chlorobi group, involved in the metabolization of mannosides and β-glucans thanks to a complete battery of endo- and exo-acting glycoside hydrolases, esterases, phosphorylases and transporters.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29601586</PMID><DateCompleted><Year>2018</Year><Month>07</Month><Day>09</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>14</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN><JournalIssue CitedMedium="Internet"><Volume>13</Volume><Issue>3</Issue><PubDate><Year>2018</Year></PubDate></JournalIssue><Title>PloS one</Title><ISOAbbreviation>PLoS One</ISOAbbreviation></Journal><ArticleTitle>Two new gene clusters involved in the degradation of plant cell wall from the fecal microbiota of Tunisian dromedary.</ArticleTitle><Pagination><MedlinePgn>e0194621</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0194621</ELocationID><Abstract><AbstractText>Dromedaries are capable of digesting plant cell wall with high content of lignocellulose of poor digestibility. Consequently, their intestinal microbiota can be a source of novel carbohydrate-active enzymes (CAZymes). To the best of our knowledge, no data are available describing the biochemical analysis of enzymes in dromedary intestinal microbiota. To investigate new hydrolytic enzymes from the dromedary gut, a fosmid library was constructed using metagenomic DNA from feces of non-domestic adult dromedary camels living in the Tunisian desert. High-throughput functional screening of 13756 clones resulted in 47 hit clones active on a panel of various chromogenic and non-chromogenic glycan substrates. Two of them, harboring multiple activities, were retained for further analysis. Clone 26H3 displayed activity on AZO-CM-cellulose, AZCL Carob galactomannan and Tween 20, while clone 36A23 was active on AZCL carob galactomannan and AZCL barley β-glucan. The functional annotation of their sequences highlighted original metagenomic loci originating from bacteria of the Bacteroidetes/Chlorobi group, involved in the metabolization of mannosides and β-glucans thanks to a complete battery of endo- and exo-acting glycoside hydrolases, esterases, phosphorylases and transporters.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ameri</LastName><ForeName>Rihab</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Laboratoire de Biotechnologie Microbienne et d'Ingénierie des Enzymes (LBMIE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Sfax, Tunisia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Laville</LastName><ForeName>Elisabeth</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>LISBP, CNRS, INRA, INSAT, Université de Toulouse, Toulouse, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Potocki-Véronèse</LastName><ForeName>Gabrielle</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>LISBP, CNRS, INRA, INSAT, Université de Toulouse, Toulouse, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Trabelsi</LastName><ForeName>Sahar</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Laboratoire de Biotechnologie Microbienne et d'Ingénierie des Enzymes (LBMIE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Sfax, Tunisia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mezghani</LastName><ForeName>Monia</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Laboratoire de Biotechnologie Microbienne et d'Ingénierie des Enzymes (LBMIE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Sfax, Tunisia.</Affiliation></AffiliationInfo></Author><Author 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