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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification of <named-content content-type="genus-species">Leishmania</named-content> by Matrix-Assisted Laser Desorption Ionization–Time of Flight (MALDI-TOF) Mass Spectrometry Using a Free Web-Based Application and a Dedicated Mass-Spectral Library</title><author><name sortKey="Lachaud, Laurence" sort="Lachaud, Laurence" uniqKey="Lachaud L" first="Laurence" last="Lachaud">Laurence Lachaud</name><affiliation><nlm:aff id="aff1">Département de Parasitologie-Mycologie, Centre National de Référence des Leishmanioses, Université de Montpellier, Centre Hospitalier Universitaire de Montpellier, Montpellier, France</nlm:aff></affiliation></author><author><name sortKey="Fernandez Arevalo, Anna" sort="Fernandez Arevalo, Anna" uniqKey="Fernandez Arevalo A" first="Anna" last="Fernández-Arévalo">Anna Fernández-Arévalo</name><affiliation><nlm:aff id="aff2">Servei de Microbiologia, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain</nlm:aff></affiliation><affiliation><nlm:aff id="aff3">Secció de Parasitologia, Facultat de Farmàcia i Ciències de l'Alimentació, Universitat de Barcelona, Barcelona, Spain</nlm:aff></affiliation><affiliation><nlm:aff id="aff4">ISGlobal, Barcelona Center of International Health Research (CRESIB), Hospital Clínic-Universitat de Barcelona, Barcelona, Spain</nlm:aff></affiliation></author><author><name sortKey="Normand, Anne Cecile" sort="Normand, Anne Cecile" uniqKey="Normand A" first="Anne-Cécile" last="Normand">Anne-Cécile Normand</name><affiliation><nlm:aff id="aff5">Laboratoire de Parasitologie-Mycologie, CHU Timone, Université d'Aix-Marseille, Marseille, France</nlm:aff></affiliation></author><author><name sortKey="Lami, Patrick" sort="Lami, Patrick" uniqKey="Lami P" first="Patrick" last="Lami">Patrick Lami</name><affiliation><nlm:aff id="aff1">Département de Parasitologie-Mycologie, Centre National de Référence des Leishmanioses, Université de Montpellier, Centre Hospitalier Universitaire de Montpellier, Montpellier, France</nlm:aff></affiliation></author><author><name sortKey="Nabet, Cecile" sort="Nabet, Cecile" uniqKey="Nabet C" first="Cécile" last="Nabet">Cécile Nabet</name><affiliation><nlm:aff id="aff6">Laboratoire Hospitalo-Universitaire de Parasito-Mycologie, EA 3593, EPaT Ecosystèmes Amazoniens et Pathologie Tropicale, Université de Guyane, Laboratoire Associé au CNR Leishmania, Cayenne, France</nlm:aff></affiliation></author><author><name sortKey="Donnadieu, Jean Luc" sort="Donnadieu, Jean Luc" uniqKey="Donnadieu J" first="Jean Luc" last="Donnadieu">Jean Luc Donnadieu</name><affiliation><nlm:aff id="aff7">Association Passerelles, Montpellier, France</nlm:aff></affiliation></author><author><name sortKey="Piarroux, Martine" sort="Piarroux, Martine" uniqKey="Piarroux M" first="Martine" last="Piarroux">Martine Piarroux</name><affiliation><nlm:aff id="aff8">Université Aix-Marseille, INSERM-IRD-AMU UMR 912, Marseille, France</nlm:aff></affiliation></author><author><name sortKey="Djenad, Farid" sort="Djenad, Farid" uniqKey="Djenad F" first="Farid" last="Djenad">Farid Djenad</name><affiliation><nlm:aff id="aff5">Laboratoire de Parasitologie-Mycologie, CHU Timone, Université d'Aix-Marseille, Marseille, France</nlm:aff></affiliation></author><author><name sortKey="Cassagne, Carole" sort="Cassagne, Carole" uniqKey="Cassagne C" first="Carole" last="Cassagne">Carole Cassagne</name><affiliation><nlm:aff id="aff5">Laboratoire de Parasitologie-Mycologie, CHU Timone, Université d'Aix-Marseille, Marseille, France</nlm:aff></affiliation></author><author><name sortKey="Ravel, Christophe" sort="Ravel, Christophe" uniqKey="Ravel C" first="Christophe" last="Ravel">Christophe Ravel</name><affiliation><nlm:aff id="aff1">Département de Parasitologie-Mycologie, Centre National de Référence des Leishmanioses, Université de Montpellier, Centre Hospitalier Universitaire de Montpellier, Montpellier, France</nlm:aff></affiliation></author><author><name sortKey="Tebar, Silvia" sort="Tebar, Silvia" uniqKey="Tebar S" first="Silvia" last="Tebar">Silvia Tebar</name><affiliation><nlm:aff id="aff3">Secció de Parasitologia, Facultat de Farmàcia i Ciències de l'Alimentació, Universitat de Barcelona, Barcelona, Spain</nlm:aff></affiliation></author><author><name sortKey="Llovet, Teresa" sort="Llovet, Teresa" uniqKey="Llovet T" first="Teresa" last="Llovet">Teresa Llovet</name><affiliation><nlm:aff id="aff2">Servei de Microbiologia, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain</nlm:aff></affiliation><affiliation><nlm:aff id="aff9">Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain</nlm:aff></affiliation></author><author><name sortKey="Blanchet, Denis" sort="Blanchet, Denis" uniqKey="Blanchet D" first="Denis" last="Blanchet">Denis Blanchet</name><affiliation><nlm:aff id="aff6">Laboratoire Hospitalo-Universitaire de Parasito-Mycologie, EA 3593, EPaT Ecosystèmes Amazoniens et Pathologie Tropicale, Université de Guyane, Laboratoire Associé au CNR Leishmania, Cayenne, France</nlm:aff></affiliation></author><author><name sortKey="Demar, Magalie" sort="Demar, Magalie" uniqKey="Demar M" first="Magalie" last="Demar">Magalie Demar</name><affiliation><nlm:aff id="aff6">Laboratoire Hospitalo-Universitaire de Parasito-Mycologie, EA 3593, EPaT Ecosystèmes Amazoniens et Pathologie Tropicale, Université de Guyane, Laboratoire Associé au CNR Leishmania, Cayenne, France</nlm:aff></affiliation></author><author><name sortKey="Harrat, Zoubir" sort="Harrat, Zoubir" uniqKey="Harrat Z" first="Zoubir" last="Harrat">Zoubir Harrat</name><affiliation><nlm:aff id="aff10">Laboratoire d'Eco-Épidemiologie Parasitaire et Génétique des Populations, Institut Pasteur d'Algerie, Algiers, Algeria</nlm:aff></affiliation></author><author><name sortKey="Aoun, Karim" sort="Aoun, Karim" uniqKey="Aoun K" first="Karim" last="Aoun">Karim Aoun</name><affiliation><nlm:aff id="aff11">Laboratoire de Parasitologie Médicale, Biotechnologies et Biomolécules, Institut Pasteur de Tunis, Tunis, Tunisia</nlm:aff></affiliation></author><author><name sortKey="Bastien, Patrick" sort="Bastien, Patrick" uniqKey="Bastien P" first="Patrick" last="Bastien">Patrick Bastien</name><affiliation><nlm:aff id="aff1">Département de Parasitologie-Mycologie, Centre National de Référence des Leishmanioses, Université de Montpellier, Centre Hospitalier Universitaire de Montpellier, Montpellier, France</nlm:aff></affiliation></author><author><name sortKey="Mu Oz, Carmen" sort="Mu Oz, Carmen" uniqKey="Mu Oz C" first="Carmen" last="Mu Oz">Carmen Mu Oz</name><affiliation><nlm:aff id="aff2">Servei de Microbiologia, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain</nlm:aff></affiliation><affiliation><nlm:aff id="aff9">Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain</nlm:aff></affiliation></author><author><name sortKey="Gallego, Montserrat" sort="Gallego, Montserrat" uniqKey="Gallego M" first="Montserrat" last="Gállego">Montserrat Gállego</name><affiliation><nlm:aff id="aff3">Secció de Parasitologia, Facultat de Farmàcia i Ciències de l'Alimentació, Universitat de Barcelona, Barcelona, Spain</nlm:aff></affiliation><affiliation><nlm:aff id="aff4">ISGlobal, Barcelona Center of International Health Research (CRESIB), Hospital Clínic-Universitat de Barcelona, Barcelona, Spain</nlm:aff></affiliation></author><author><name sortKey="Piarroux, Renaud" sort="Piarroux, Renaud" uniqKey="Piarroux R" first="Renaud" last="Piarroux">Renaud Piarroux</name><affiliation><nlm:aff id="aff5">Laboratoire de Parasitologie-Mycologie, CHU Timone, Université d'Aix-Marseille, Marseille, France</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">28724559</idno><idno type="pmc">5625378</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5625378</idno><idno type="RBID">PMC:5625378</idno><idno type="doi">10.1128/JCM.00845-17</idno><date when="2017">2017</date><idno type="wicri:Area/Pmc/Corpus">000135</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000135</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Identification of <named-content content-type="genus-species">Leishmania</named-content> by Matrix-Assisted Laser Desorption Ionization–Time of Flight (MALDI-TOF) Mass Spectrometry Using a Free Web-Based Application and a Dedicated Mass-Spectral Library</title><author><name sortKey="Lachaud, Laurence" sort="Lachaud, Laurence" uniqKey="Lachaud L" first="Laurence" last="Lachaud">Laurence Lachaud</name><affiliation><nlm:aff id="aff1">Département de Parasitologie-Mycologie, Centre National de Référence des Leishmanioses, Université de Montpellier, Centre Hospitalier Universitaire de Montpellier, Montpellier, France</nlm:aff></affiliation></author><author><name sortKey="Fernandez Arevalo, Anna" sort="Fernandez Arevalo, Anna" uniqKey="Fernandez Arevalo A" first="Anna" last="Fernández-Arévalo">Anna Fernández-Arévalo</name><affiliation><nlm:aff id="aff2">Servei de Microbiologia, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain</nlm:aff></affiliation><affiliation><nlm:aff id="aff3">Secció de Parasitologia, Facultat de Farmàcia i Ciències de l'Alimentació, Universitat de Barcelona, Barcelona, Spain</nlm:aff></affiliation><affiliation><nlm:aff id="aff4">ISGlobal, Barcelona Center of International Health Research (CRESIB), Hospital Clínic-Universitat de Barcelona, Barcelona, Spain</nlm:aff></affiliation></author><author><name sortKey="Normand, Anne Cecile" sort="Normand, Anne Cecile" uniqKey="Normand A" first="Anne-Cécile" last="Normand">Anne-Cécile Normand</name><affiliation><nlm:aff id="aff5">Laboratoire de Parasitologie-Mycologie, CHU Timone, Université d'Aix-Marseille, Marseille, France</nlm:aff></affiliation></author><author><name sortKey="Lami, Patrick" sort="Lami, Patrick" uniqKey="Lami P" first="Patrick" last="Lami">Patrick Lami</name><affiliation><nlm:aff id="aff1">Département de Parasitologie-Mycologie, Centre National de Référence des Leishmanioses, Université de Montpellier, Centre Hospitalier Universitaire de Montpellier, Montpellier, France</nlm:aff></affiliation></author><author><name sortKey="Nabet, Cecile" sort="Nabet, Cecile" uniqKey="Nabet C" first="Cécile" last="Nabet">Cécile Nabet</name><affiliation><nlm:aff id="aff6">Laboratoire Hospitalo-Universitaire de Parasito-Mycologie, EA 3593, EPaT Ecosystèmes Amazoniens et Pathologie Tropicale, Université de Guyane, Laboratoire Associé au CNR Leishmania, Cayenne, France</nlm:aff></affiliation></author><author><name sortKey="Donnadieu, Jean Luc" sort="Donnadieu, Jean Luc" uniqKey="Donnadieu J" first="Jean Luc" last="Donnadieu">Jean Luc Donnadieu</name><affiliation><nlm:aff id="aff7">Association Passerelles, Montpellier, France</nlm:aff></affiliation></author><author><name sortKey="Piarroux, Martine" sort="Piarroux, Martine" uniqKey="Piarroux M" first="Martine" last="Piarroux">Martine Piarroux</name><affiliation><nlm:aff id="aff8">Université Aix-Marseille, INSERM-IRD-AMU UMR 912, Marseille, France</nlm:aff></affiliation></author><author><name sortKey="Djenad, Farid" sort="Djenad, Farid" uniqKey="Djenad F" first="Farid" last="Djenad">Farid Djenad</name><affiliation><nlm:aff id="aff5">Laboratoire de Parasitologie-Mycologie, CHU Timone, Université d'Aix-Marseille, Marseille, France</nlm:aff></affiliation></author><author><name sortKey="Cassagne, Carole" sort="Cassagne, Carole" uniqKey="Cassagne C" first="Carole" last="Cassagne">Carole Cassagne</name><affiliation><nlm:aff id="aff5">Laboratoire de Parasitologie-Mycologie, CHU Timone, Université d'Aix-Marseille, Marseille, France</nlm:aff></affiliation></author><author><name sortKey="Ravel, Christophe" sort="Ravel, Christophe" uniqKey="Ravel C" first="Christophe" last="Ravel">Christophe Ravel</name><affiliation><nlm:aff id="aff1">Département de Parasitologie-Mycologie, Centre National de Référence des Leishmanioses, Université de Montpellier, Centre Hospitalier Universitaire de Montpellier, Montpellier, France</nlm:aff></affiliation></author><author><name sortKey="Tebar, Silvia" sort="Tebar, Silvia" uniqKey="Tebar S" first="Silvia" last="Tebar">Silvia Tebar</name><affiliation><nlm:aff id="aff3">Secció de Parasitologia, Facultat de Farmàcia i Ciències de l'Alimentació, Universitat de Barcelona, Barcelona, Spain</nlm:aff></affiliation></author><author><name sortKey="Llovet, Teresa" sort="Llovet, Teresa" uniqKey="Llovet T" first="Teresa" last="Llovet">Teresa Llovet</name><affiliation><nlm:aff id="aff2">Servei de Microbiologia, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain</nlm:aff></affiliation><affiliation><nlm:aff id="aff9">Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain</nlm:aff></affiliation></author><author><name sortKey="Blanchet, Denis" sort="Blanchet, Denis" uniqKey="Blanchet D" first="Denis" last="Blanchet">Denis Blanchet</name><affiliation><nlm:aff id="aff6">Laboratoire Hospitalo-Universitaire de Parasito-Mycologie, EA 3593, EPaT Ecosystèmes Amazoniens et Pathologie Tropicale, Université de Guyane, Laboratoire Associé au CNR Leishmania, Cayenne, France</nlm:aff></affiliation></author><author><name sortKey="Demar, Magalie" sort="Demar, Magalie" uniqKey="Demar M" first="Magalie" last="Demar">Magalie Demar</name><affiliation><nlm:aff id="aff6">Laboratoire Hospitalo-Universitaire de Parasito-Mycologie, EA 3593, EPaT Ecosystèmes Amazoniens et Pathologie Tropicale, Université de Guyane, Laboratoire Associé au CNR Leishmania, Cayenne, France</nlm:aff></affiliation></author><author><name sortKey="Harrat, Zoubir" sort="Harrat, Zoubir" uniqKey="Harrat Z" first="Zoubir" last="Harrat">Zoubir Harrat</name><affiliation><nlm:aff id="aff10">Laboratoire d'Eco-Épidemiologie Parasitaire et Génétique des Populations, Institut Pasteur d'Algerie, Algiers, Algeria</nlm:aff></affiliation></author><author><name sortKey="Aoun, Karim" sort="Aoun, Karim" uniqKey="Aoun K" first="Karim" last="Aoun">Karim Aoun</name><affiliation><nlm:aff id="aff11">Laboratoire de Parasitologie Médicale, Biotechnologies et Biomolécules, Institut Pasteur de Tunis, Tunis, Tunisia</nlm:aff></affiliation></author><author><name sortKey="Bastien, Patrick" sort="Bastien, Patrick" uniqKey="Bastien P" first="Patrick" last="Bastien">Patrick Bastien</name><affiliation><nlm:aff id="aff1">Département de Parasitologie-Mycologie, Centre National de Référence des Leishmanioses, Université de Montpellier, Centre Hospitalier Universitaire de Montpellier, Montpellier, France</nlm:aff></affiliation></author><author><name sortKey="Mu Oz, Carmen" sort="Mu Oz, Carmen" uniqKey="Mu Oz C" first="Carmen" last="Mu Oz">Carmen Mu Oz</name><affiliation><nlm:aff id="aff2">Servei de Microbiologia, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain</nlm:aff></affiliation><affiliation><nlm:aff id="aff9">Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain</nlm:aff></affiliation></author><author><name sortKey="Gallego, Montserrat" sort="Gallego, Montserrat" uniqKey="Gallego M" first="Montserrat" last="Gállego">Montserrat Gállego</name><affiliation><nlm:aff id="aff3">Secció de Parasitologia, Facultat de Farmàcia i Ciències de l'Alimentació, Universitat de Barcelona, Barcelona, Spain</nlm:aff></affiliation><affiliation><nlm:aff id="aff4">ISGlobal, Barcelona Center of International Health Research (CRESIB), Hospital Clínic-Universitat de Barcelona, Barcelona, Spain</nlm:aff></affiliation></author><author><name sortKey="Piarroux, Renaud" sort="Piarroux, Renaud" uniqKey="Piarroux R" first="Renaud" last="Piarroux">Renaud Piarroux</name><affiliation><nlm:aff id="aff5">Laboratoire de Parasitologie-Mycologie, CHU Timone, Université d'Aix-Marseille, Marseille, France</nlm:aff></affiliation></author></analytic><series><title level="j">Journal of Clinical Microbiology</title><idno type="ISSN">0095-1137</idno><idno type="eISSN">1098-660X</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>ABSTRACT</title><p>Human leishmaniases are widespread diseases with different clinical forms caused by about 20 species within the <named-content content-type="genus-species">Leishmania</named-content> genus. <named-content content-type="genus-species">Leishmania</named-content> species identification is relevant for therapeutic management and prognosis, especially for cutaneous and mucocutaneous forms. Several methods are available to identify <named-content content-type="genus-species">Leishmania</named-content> species from culture, but they have not been standardized for the majority of the currently described species, with the exception of multilocus enzyme electrophoresis. Moreover, these techniques are expensive, time-consuming, and not available in all laboratories. Within the last decade, mass spectrometry (MS) has been adapted for the identification of microorganisms, including <named-content content-type="genus-species">Leishmania</named-content>. However, no commercial reference mass-spectral database is available. In this study, a reference mass-spectral library (MSL) for <named-content content-type="genus-species">Leishmania</named-content> isolates, accessible through a free Web-based application (mass-spectral identification [MSI]), was constructed and tested. It includes mass-spectral data for 33 different <named-content content-type="genus-species">Leishmania</named-content> species, including species that infect humans, animals, and phlebotomine vectors. Four laboratories on two continents evaluated the performance of MSI using 268 samples, 231 of which were <named-content content-type="genus-species">Leishmania</named-content> strains. All <named-content content-type="genus-species">Leishmania</named-content> strains, but one, were correctly identified at least to the complex level. A risk of species misidentification within the <named-content content-type="genus-species">Leishmania donovani</named-content>, <named-content content-type="genus-species">L. guyanensis</named-content>, and <named-content content-type="genus-species">L. braziliensis</named-content> complexes was observed, as previously reported for other techniques. The tested application was reliable, with identification results being comparable to those obtained with reference methods but with a more favorable cost-efficiency ratio. This free online identification system relies on a scalable database and can be implemented directly in users' computers.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Clin Microbiol</journal-id><journal-id journal-id-type="iso-abbrev">J. Clin. Microbiol</journal-id><journal-id journal-id-type="hwp">jcm</journal-id><journal-id journal-id-type="pmc">jcm</journal-id><journal-id journal-id-type="publisher-id">JCM</journal-id><journal-title-group><journal-title>Journal of Clinical Microbiology</journal-title></journal-title-group><issn pub-type="ppub">0095-1137</issn><issn pub-type="epub">1098-660X</issn><publisher><publisher-name>American Society for Microbiology</publisher-name><publisher-loc>1752 N St., N.W., Washington, DC</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">28724559</article-id><article-id pub-id-type="pmc">5625378</article-id><article-id pub-id-type="publisher-id">00845-17</article-id><article-id pub-id-type="doi">10.1128/JCM.00845-17</article-id><article-categories><subj-group subj-group-type="heading"><subject>Parasitology</subject></subj-group></article-categories><title-group><article-title>Identification of <named-content content-type="genus-species">Leishmania</named-content> by Matrix-Assisted Laser Desorption Ionization–Time of Flight (MALDI-TOF) Mass Spectrometry Using a Free Web-Based Application and a Dedicated Mass-Spectral Library</article-title><alt-title alt-title-type="running-head">MALDI-TOF-Based <named-content content-type="genus-species">Leishmania</named-content> Identification Online</alt-title><alt-title alt-title-type="short-authors">Lachaud et al.</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Lachaud</surname><given-names>Laurence</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Fernández-Arévalo</surname><given-names>Anna</given-names></name><xref ref-type="aff" rid="aff2"><sup>b</sup></xref><xref ref-type="aff" rid="aff3"><sup>c</sup></xref><xref ref-type="aff" rid="aff4"><sup>d</sup></xref></contrib><contrib contrib-type="author"><name><surname>Normand</surname><given-names>Anne-Cécile</given-names></name><xref ref-type="aff" rid="aff5"><sup>e</sup></xref></contrib><contrib contrib-type="author"><name><surname>Lami</surname><given-names>Patrick</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Nabet</surname><given-names>Cécile</given-names></name><xref ref-type="aff" rid="aff6"><sup>f</sup></xref></contrib><contrib contrib-type="author"><name><surname>Donnadieu</surname><given-names>Jean Luc</given-names></name><xref ref-type="aff" rid="aff7"><sup>g</sup></xref></contrib><contrib contrib-type="author"><name><surname>Piarroux</surname><given-names>Martine</given-names></name><xref ref-type="aff" rid="aff8"><sup>h</sup></xref></contrib><contrib contrib-type="author"><name><surname>Djenad</surname><given-names>Farid</given-names></name><xref ref-type="aff" rid="aff5"><sup>e</sup></xref></contrib><contrib contrib-type="author"><name><surname>Cassagne</surname><given-names>Carole</given-names></name><xref ref-type="aff" rid="aff5"><sup>e</sup></xref></contrib><contrib contrib-type="author"><name><surname>Ravel</surname><given-names>Christophe</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Tebar</surname><given-names>Silvia</given-names></name><xref ref-type="aff" rid="aff3"><sup>c</sup></xref></contrib><contrib contrib-type="author"><name><surname>Llovet</surname><given-names>Teresa</given-names></name><xref ref-type="aff" rid="aff2"><sup>b</sup></xref><xref ref-type="aff" rid="aff9"><sup>i</sup></xref></contrib><contrib contrib-type="author"><name><surname>Blanchet</surname><given-names>Denis</given-names></name><xref ref-type="aff" rid="aff6"><sup>f</sup></xref></contrib><contrib contrib-type="author"><name><surname>Demar</surname><given-names>Magalie</given-names></name><xref ref-type="aff" rid="aff6"><sup>f</sup></xref></contrib><contrib contrib-type="author"><name><surname>Harrat</surname><given-names>Zoubir</given-names></name><xref ref-type="aff" rid="aff10"><sup>j</sup></xref></contrib><contrib contrib-type="author"><name><surname>Aoun</surname><given-names>Karim</given-names></name><xref ref-type="aff" rid="aff11"><sup>k</sup></xref></contrib><contrib contrib-type="author"><name><surname>Bastien</surname><given-names>Patrick</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Muñoz</surname><given-names>Carmen</given-names></name><xref ref-type="aff" rid="aff2"><sup>b</sup></xref><xref ref-type="aff" rid="aff9"><sup>i</sup></xref></contrib><contrib contrib-type="author"><name><surname>Gállego</surname><given-names>Montserrat</given-names></name><xref ref-type="aff" rid="aff3"><sup>c</sup></xref><xref ref-type="aff" rid="aff4"><sup>d</sup></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Piarroux</surname><given-names>Renaud</given-names></name><xref ref-type="aff" rid="aff5"><sup>e</sup></xref></contrib><aff id="aff1"><label>a</label>Département de Parasitologie-Mycologie, Centre National de Référence des Leishmanioses, Université de Montpellier, Centre Hospitalier Universitaire de Montpellier, Montpellier, France</aff><aff id="aff2"><label>b</label>Servei de Microbiologia, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain</aff><aff id="aff3"><label>c</label>Secció de Parasitologia, Facultat de Farmàcia i Ciències de l'Alimentació, Universitat de Barcelona, Barcelona, Spain</aff><aff id="aff4"><label>d</label>ISGlobal, Barcelona Center of International Health Research (CRESIB), Hospital Clínic-Universitat de Barcelona, Barcelona, Spain</aff><aff id="aff5"><label>e</label>Laboratoire de Parasitologie-Mycologie, CHU Timone, Université d'Aix-Marseille, Marseille, France</aff><aff id="aff6"><label>f</label>Laboratoire Hospitalo-Universitaire de Parasito-Mycologie, EA 3593, EPaT Ecosystèmes Amazoniens et Pathologie Tropicale, Université de Guyane, Laboratoire Associé au CNR Leishmania, Cayenne, France</aff><aff id="aff7"><label>g</label>Association Passerelles, Montpellier, France</aff><aff id="aff8"><label>h</label>Université Aix-Marseille, INSERM-IRD-AMU UMR 912, Marseille, France</aff><aff id="aff9"><label>i</label>Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain</aff><aff id="aff10"><label>j</label>Laboratoire d'Eco-Épidemiologie Parasitaire et Génétique des Populations, Institut Pasteur d'Algerie, Algiers, Algeria</aff><aff id="aff11"><label>k</label>Laboratoire de Parasitologie Médicale, Biotechnologies et Biomolécules, Institut Pasteur de Tunis, Tunis, Tunisia</aff></contrib-group><contrib-group><contrib contrib-type="editor"><name><surname>Loeffelholz</surname><given-names>Michael J.</given-names></name><role>Editor</role><aff>University of Texas Medical Branch</aff></contrib></contrib-group><author-notes><corresp id="cor1">Address correspondence to Renaud Piarroux, <email>mpiarroux@yahoo.fr</email>.</corresp><fn fn-type="equal"><p>L.L. and A.F.-A. contributed equally to this work.</p></fn><fn fn-type="other"><p><bold>Citation</bold> Lachaud L, Fernández-Arévalo A, Normand A-C, Lami P, Nabet C, Donnadieu JL, Piarroux M, Djenad F, Cassagne C, Ravel C, Tebar S, Llovet T, Blanchet D, Demar M, Harrat Z, Aoun K, Bastien P, Muñoz C, Gállego M, Piarroux R. 2017. Identification of <named-content content-type="genus-species">Leishmania</named-content> by matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry using a free Web-based application and a dedicated mass-spectral library. J Clin Microbiol 55:2924–2933. <ext-link ext-link-type="uri" xlink:href="https://doi.org/10.1128/JCM.00845-17">https://doi.org/10.1128/JCM.00845-17</ext-link>.</p></fn></author-notes><pub-date pub-type="epreprint"><day>19</day><month>7</month><year>2017</year></pub-date><pub-date pub-type="epub"><day>25</day><month>9</month><year>2017</year></pub-date><pub-date pub-type="ppub"><month>10</month><year>2017</year></pub-date><volume>55</volume><issue>10</issue><fpage>2924</fpage><lpage>2933</lpage><history><date date-type="received"><day>31</day><month>5</month><year>2017</year></date><date date-type="rev-request"><day>27</day><month>6</month><year>2017</year></date><date date-type="accepted"><day>11</day><month>7</month><year>2017</year></date></history><permissions><copyright-statement>Copyright © 2017 American Society for Microbiology.</copyright-statement><copyright-year>2017</copyright-year><copyright-holder>American Society for Microbiology</copyright-holder><license license-type="asm" xlink:href="https://doi.org/10.1128/ASMCopyrightv1"><license-p><ext-link ext-link-type="uri" xlink:href="https://doi.org/10.1128/ASMCopyrightv1">All Rights Reserved</ext-link>.</license-p></license></permissions><self-uri content-type="pdf" xlink:href="zjm01017002924.pdf"></self-uri><abstract><title>ABSTRACT</title><p>Human leishmaniases are widespread diseases with different clinical forms caused by about 20 species within the <named-content content-type="genus-species">Leishmania</named-content> genus. <named-content content-type="genus-species">Leishmania</named-content> species identification is relevant for therapeutic management and prognosis, especially for cutaneous and mucocutaneous forms. Several methods are available to identify <named-content content-type="genus-species">Leishmania</named-content> species from culture, but they have not been standardized for the majority of the currently described species, with the exception of multilocus enzyme electrophoresis. Moreover, these techniques are expensive, time-consuming, and not available in all laboratories. Within the last decade, mass spectrometry (MS) has been adapted for the identification of microorganisms, including <named-content content-type="genus-species">Leishmania</named-content>. However, no commercial reference mass-spectral database is available. In this study, a reference mass-spectral library (MSL) for <named-content content-type="genus-species">Leishmania</named-content> isolates, accessible through a free Web-based application (mass-spectral identification [MSI]), was constructed and tested. It includes mass-spectral data for 33 different <named-content content-type="genus-species">Leishmania</named-content> species, including species that infect humans, animals, and phlebotomine vectors. Four laboratories on two continents evaluated the performance of MSI using 268 samples, 231 of which were <named-content content-type="genus-species">Leishmania</named-content> strains. All <named-content content-type="genus-species">Leishmania</named-content> strains, but one, were correctly identified at least to the complex level. A risk of species misidentification within the <named-content content-type="genus-species">Leishmania donovani</named-content>, <named-content content-type="genus-species">L. guyanensis</named-content>, and <named-content content-type="genus-species">L. braziliensis</named-content> complexes was observed, as previously reported for other techniques. The tested application was reliable, with identification results being comparable to those obtained with reference methods but with a more favorable cost-efficiency ratio. This free online identification system relies on a scalable database and can be implemented directly in users' computers.</p></abstract><kwd-group><title>KEYWORDS</title><kwd><named-content content-type="genus-species">Leishmania</named-content></kwd><kwd>MALDI-TOF</kwd><kwd>database</kwd><kwd>identification online</kwd></kwd-group><funding-group><award-group id="award1"><funding-source id="gs1">Santé publique France</funding-source><principal-award-recipient>Laurence Lachaud</principal-award-recipient></award-group></funding-group><counts><fig-count count="1"></fig-count><table-count count="4"></table-count><equation-count count="0"></equation-count><ref-count count="36"></ref-count><page-count count="10"></page-count><word-count count="6541"></word-count></counts><custom-meta-group><custom-meta><meta-name>cover-date</meta-name><meta-value>October 2017</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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