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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification of Differentially Expressed Genes by
cDNA-AFLP Technique in Response to Drought Stress
in <italic>Triticum durum</italic></title><author><name sortKey="Melloul, Marouane" sort="Melloul, Marouane" uniqKey="Melloul M" first="Marouane" last="Melloul">Marouane Melloul</name><affiliation><nlm:aff id="aff1">Genetic and Biometry Laboratory, Faculty of Sciences, University Ibn Tofail, BP 133,
14000 Kenitra,<country>Morocco</country></nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Functional Genomic Platform, Technical Unit (UATRS), National Center for Scientific and Technical Research (CNRST), Angle Allal Fassi, Avenue des FAR, Hay Riad, BP 8027, 10102 Rabat,<country>Morocco</country></nlm:aff></affiliation></author><author><name sortKey="Iraqi, Driss" sort="Iraqi, Driss" uniqKey="Iraqi D" first="Driss" last="Iraqi">Driss Iraqi</name><affiliation><nlm:aff id="aff3">National Institute of Agronomical Research, Avenue de la Victoire, BP 415, Rabat,<country>Morocco</country></nlm:aff></affiliation></author><author><name sortKey="El Alaoui, Myabdelaziz" sort="El Alaoui, Myabdelaziz" uniqKey="El Alaoui M" first="Myabdelaziz" last="El Alaoui">Myabdelaziz El Alaoui</name><affiliation><nlm:aff id="aff1">Genetic and Biometry Laboratory, Faculty of Sciences, University Ibn Tofail, BP 133,
14000 Kenitra,<country>Morocco</country></nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Functional Genomic Platform, Technical Unit (UATRS), National Center for Scientific and Technical Research (CNRST), Angle Allal Fassi, Avenue des FAR, Hay Riad, BP 8027, 10102 Rabat,<country>Morocco</country></nlm:aff></affiliation></author><author><name sortKey="Erba, Gilles" sort="Erba, Gilles" uniqKey="Erba G" first="Gilles" last="Erba">Gilles Erba</name><affiliation><nlm:aff id="aff4">Labgene Scientific Instruments, Athens Building, Business Park, 74160 Archamps,<country>France</country></nlm:aff></affiliation></author><author><name sortKey="Alaoui, Sanaa" sort="Alaoui, Sanaa" uniqKey="Alaoui S" first="Sanaa" last="Alaoui">Sanaa Alaoui</name><affiliation><nlm:aff id="aff2">Functional Genomic Platform, Technical Unit (UATRS), National Center for Scientific and Technical Research (CNRST), Angle Allal Fassi, Avenue des FAR, Hay Riad, BP 8027, 10102 Rabat,<country>Morocco</country></nlm:aff></affiliation></author><author><name sortKey="Ibriz, Mohammed" sort="Ibriz, Mohammed" uniqKey="Ibriz M" first="Mohammed" last="Ibriz">Mohammed Ibriz</name><affiliation><nlm:aff id="aff1">Genetic and Biometry Laboratory, Faculty of Sciences, University Ibn Tofail, BP 133,
14000 Kenitra,<country>Morocco</country></nlm:aff></affiliation></author><author><name sortKey="Elfahime, Elmostafa" sort="Elfahime, Elmostafa" uniqKey="Elfahime E" first="Elmostafa" last="Elfahime">Elmostafa Elfahime</name><affiliation><nlm:aff id="aff2">Functional Genomic Platform, Technical Unit (UATRS), National Center for Scientific and Technical Research (CNRST), Angle Allal Fassi, Avenue des FAR, Hay Riad, BP 8027, 10102 Rabat,<country>Morocco</country></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27904321</idno><idno type="pmc">5079143</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5079143</idno><idno type="RBID">PMC:5079143</idno><idno type="doi">10.17113/ftb.52.04.14.3701</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000132</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000132</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Identification of Differentially Expressed Genes by
cDNA-AFLP Technique in Response to Drought Stress
in <italic>Triticum durum</italic></title><author><name sortKey="Melloul, Marouane" sort="Melloul, Marouane" uniqKey="Melloul M" first="Marouane" last="Melloul">Marouane Melloul</name><affiliation><nlm:aff id="aff1">Genetic and Biometry Laboratory, Faculty of Sciences, University Ibn Tofail, BP 133,
14000 Kenitra,<country>Morocco</country></nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Functional Genomic Platform, Technical Unit (UATRS), National Center for Scientific and Technical Research (CNRST), Angle Allal Fassi, Avenue des FAR, Hay Riad, BP 8027, 10102 Rabat,<country>Morocco</country></nlm:aff></affiliation></author><author><name sortKey="Iraqi, Driss" sort="Iraqi, Driss" uniqKey="Iraqi D" first="Driss" last="Iraqi">Driss Iraqi</name><affiliation><nlm:aff id="aff3">National Institute of Agronomical Research, Avenue de la Victoire, BP 415, Rabat,<country>Morocco</country></nlm:aff></affiliation></author><author><name sortKey="El Alaoui, Myabdelaziz" sort="El Alaoui, Myabdelaziz" uniqKey="El Alaoui M" first="Myabdelaziz" last="El Alaoui">Myabdelaziz El Alaoui</name><affiliation><nlm:aff id="aff1">Genetic and Biometry Laboratory, Faculty of Sciences, University Ibn Tofail, BP 133,
14000 Kenitra,<country>Morocco</country></nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Functional Genomic Platform, Technical Unit (UATRS), National Center for Scientific and Technical Research (CNRST), Angle Allal Fassi, Avenue des FAR, Hay Riad, BP 8027, 10102 Rabat,<country>Morocco</country></nlm:aff></affiliation></author><author><name sortKey="Erba, Gilles" sort="Erba, Gilles" uniqKey="Erba G" first="Gilles" last="Erba">Gilles Erba</name><affiliation><nlm:aff id="aff4">Labgene Scientific Instruments, Athens Building, Business Park, 74160 Archamps,<country>France</country></nlm:aff></affiliation></author><author><name sortKey="Alaoui, Sanaa" sort="Alaoui, Sanaa" uniqKey="Alaoui S" first="Sanaa" last="Alaoui">Sanaa Alaoui</name><affiliation><nlm:aff id="aff2">Functional Genomic Platform, Technical Unit (UATRS), National Center for Scientific and Technical Research (CNRST), Angle Allal Fassi, Avenue des FAR, Hay Riad, BP 8027, 10102 Rabat,<country>Morocco</country></nlm:aff></affiliation></author><author><name sortKey="Ibriz, Mohammed" sort="Ibriz, Mohammed" uniqKey="Ibriz M" first="Mohammed" last="Ibriz">Mohammed Ibriz</name><affiliation><nlm:aff id="aff1">Genetic and Biometry Laboratory, Faculty of Sciences, University Ibn Tofail, BP 133,
14000 Kenitra,<country>Morocco</country></nlm:aff></affiliation></author><author><name sortKey="Elfahime, Elmostafa" sort="Elfahime, Elmostafa" uniqKey="Elfahime E" first="Elmostafa" last="Elfahime">Elmostafa Elfahime</name><affiliation><nlm:aff id="aff2">Functional Genomic Platform, Technical Unit (UATRS), National Center for Scientific and Technical Research (CNRST), Angle Allal Fassi, Avenue des FAR, Hay Riad, BP 8027, 10102 Rabat,<country>Morocco</country></nlm:aff></affiliation></author></analytic><series><title level="j">Food Technology and Biotechnology</title><idno type="ISSN">1330-9862</idno><idno type="eISSN">1334-2606</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Summary</title><p>Drought is the single largest abiotic stress factor leading to reduced crop yields. The identification of differentially expressed genes and the understanding of their functions in environmentally stressful conditions are essential to improve drought tolerance. Transcriptomics is a powerful approach for the global analysis of molecular mechanisms under abiotic stress. To identify genes that are important for drought tolerance, we analyzed mRNA populations from untreated and drought-stressed leaves of <italic>Triticum durum</italic> by cDNA- -amplified fragment length polymorphism (cDNA-AFLP) technique. Overall, 76 transcript- -derived fragments corresponding to differentially induced transcripts were successfully sequenced. Most of the transcripts identified here, using basic local alignment search tool (BLAST) database, were genes belonging to different functional categories related to metabolism, energy, cellular biosynthesis, cell defense, signal transduction, transcription regulation, protein degradation and transport. The expression patterns of these genes were confirmed by quantitative reverse transcriptase real-time polymerase chain reaction (qRT- -PCR) based on ten selected genes representing different patterns. These results could facilitate the understanding of cellular mechanisms involving groups of genes that act in coordination in response to stimuli of water deficit. The identification of novel stress-responsive genes will provide useful data that could help develop breeding strategies aimed at improving durum wheat tolerance to field stress.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Food Technol Biotechnol</journal-id><journal-id journal-id-type="iso-abbrev">Food Technol. Biotechnol</journal-id><journal-id journal-id-type="publisher-id">FTB</journal-id><journal-title-group><journal-title>Food Technology and Biotechnology</journal-title></journal-title-group><issn pub-type="ppub">1330-9862</issn><issn pub-type="epub">1334-2606</issn><publisher><publisher-name>University of Zagreb Faculty of Food Technology and Biotechnology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">27904321</article-id><article-id pub-id-type="pmc">5079143</article-id><article-id pub-id-type="publisher-id">ftb-52-479</article-id><article-id pub-id-type="doi">10.17113/ftb.52.04.14.3701</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Scientific Paper</subject></subj-group></article-categories><title-group><article-title>Identification of Differentially Expressed Genes by
cDNA-AFLP Technique in Response to Drought Stress
in <italic>Triticum durum</italic></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Melloul</surname><given-names>Marouane</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Iraqi</surname><given-names>Driss</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>El Alaoui</surname><given-names>MyAbdelaziz</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Erba</surname><given-names>Gilles</given-names></name><xref ref-type="aff" rid="aff4"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Alaoui</surname><given-names>Sanaa</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Ibriz</surname><given-names>Mohammed</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Elfahime</surname><given-names>Elmostafa</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><aff id="aff1"><label>1</label>Genetic and Biometry Laboratory, Faculty of Sciences, University Ibn Tofail, BP 133,
14000 Kenitra,<country>Morocco</country></aff><aff id="aff2"><label>2</label>Functional Genomic Platform, Technical Unit (UATRS), National Center for Scientific and Technical Research (CNRST), Angle Allal Fassi, Avenue des FAR, Hay Riad, BP 8027, 10102 Rabat,<country>Morocco</country></aff><aff id="aff3"><label>3</label>National Institute of Agronomical Research, Avenue de la Victoire, BP 415, Rabat,<country>Morocco</country></aff><aff id="aff4"><label>4</label>Labgene Scientific Instruments, Athens Building, Business Park, 74160 Archamps,<country>France</country></aff></contrib-group><author-notes><corresp id="cor1">Corresponding author: Elmostafa Elfahime
Phone: +212 537 712 983;
Fax: +212 537 713 205;
E-mail: <email xlink:href="elfahime.elmostafa@gmail.com">elfahime.elmostafa@gmail.com</email>, <email xlink:href="elfahime@cnrst.ma">elfahime@cnrst.ma</email></corresp></author-notes><pub-date pub-type="ppub"><month>12</month><year>2014</year></pub-date><volume>52</volume><issue>4</issue><fpage>479</fpage><lpage>488</lpage><history><date date-type="received"><day>25</day><month>4</month><year>2014</year></date><date date-type="accepted"><day>12</day><month>8</month><year>2014</year></date></history><permissions><copyright-year>2014</copyright-year><copyright-holder>University of Zagreb Faculty of Food Technology and Biotechnology</copyright-holder></permissions><abstract><title>Summary</title><p>Drought is the single largest abiotic stress factor leading to reduced crop yields. The identification of differentially expressed genes and the understanding of their functions in environmentally stressful conditions are essential to improve drought tolerance. Transcriptomics is a powerful approach for the global analysis of molecular mechanisms under abiotic stress. To identify genes that are important for drought tolerance, we analyzed mRNA populations from untreated and drought-stressed leaves of <italic>Triticum durum</italic> by cDNA- -amplified fragment length polymorphism (cDNA-AFLP) technique. Overall, 76 transcript- -derived fragments corresponding to differentially induced transcripts were successfully sequenced. Most of the transcripts identified here, using basic local alignment search tool (BLAST) database, were genes belonging to different functional categories related to metabolism, energy, cellular biosynthesis, cell defense, signal transduction, transcription regulation, protein degradation and transport. The expression patterns of these genes were confirmed by quantitative reverse transcriptase real-time polymerase chain reaction (qRT- -PCR) based on ten selected genes representing different patterns. These results could facilitate the understanding of cellular mechanisms involving groups of genes that act in coordination in response to stimuli of water deficit. The identification of novel stress-responsive genes will provide useful data that could help develop breeding strategies aimed at improving durum wheat tolerance to field stress.</p></abstract><kwd-group kwd-group-type="author"><title>Key words: </title><kwd>cDNA-AFLP</kwd><kwd>drought stress</kwd><kwd>transcript-derived fragments</kwd><kwd>durum wheat</kwd><kwd>real- -time PCR</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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