New microsatellite loci for Narcissus papyraceus (Amarillydaceae) and cross-amplification in other congeneric species.
Identifieur interne : 000129 ( Main/Merge ); précédent : 000128; suivant : 000130New microsatellite loci for Narcissus papyraceus (Amarillydaceae) and cross-amplification in other congeneric species.
Auteurs : Violeta I. Sim N [Espagne] ; F Xavier Pic ; Juan ArroyoSource :
- American journal of botany [ 0002-9122 ] ; 2010.
Abstract
UNLABELLED
PREMISE OF THE STUDY
Microsatellite loci from a genomic library of the species Narcissus papyraceus were optimized and characterized for studies of population genetics. •
METHODS AND RESULTS
Eleven markers that were successfully amplified showed polymorphism when tested on 50 individuals from two populations in southern Spain and northern Morocco. Overall, the number of alleles per locus ranged between 4 and 15. Between 8 and 11 loci successfully amplified in other eight Narcissus species. •
CONCLUSIONS
These markers will enable genetic diversity studies of N. papyraceus across its distribution range and conduct paternity analyses among individuals differing in flower morphology.
DOI: 10.3732/ajb.1000023
PubMed: 21622397
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Sim N</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Biología Vegetal y Ecología, Universidad de Sevilla, Sevilla, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Biología Vegetal y Ecología, Universidad de Sevilla, Sevilla</wicri:regionArea><wicri:noRegion>Sevilla</wicri:noRegion></affiliation></author><author><name sortKey="Pic, F Xavier" sort="Pic, F Xavier" uniqKey="Pic F" first="F Xavier" last="Pic">F Xavier Pic</name></author><author><name sortKey="Arroyo, Juan" sort="Arroyo, Juan" uniqKey="Arroyo J" first="Juan" last="Arroyo">Juan Arroyo</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:21622397</idno><idno type="pmid">21622397</idno><idno type="doi">10.3732/ajb.1000023</idno><idno type="wicri:Area/PubMed/Corpus">000718</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000718</idno><idno type="wicri:Area/PubMed/Curation">000714</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000714</idno><idno type="wicri:Area/PubMed/Checkpoint">000706</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000706</idno><idno type="wicri:Area/Main/Merge">000129</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">New microsatellite loci for Narcissus papyraceus (Amarillydaceae) and cross-amplification in other congeneric species.</title><author><name sortKey="Sim N, Violeta I" sort="Sim N, Violeta I" uniqKey="Sim N V" first="Violeta I" last="Sim N">Violeta I. Sim N</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Biología Vegetal y Ecología, Universidad de Sevilla, Sevilla, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Biología Vegetal y Ecología, Universidad de Sevilla, Sevilla</wicri:regionArea><wicri:noRegion>Sevilla</wicri:noRegion></affiliation></author><author><name sortKey="Pic, F Xavier" sort="Pic, F Xavier" uniqKey="Pic F" first="F Xavier" last="Pic">F Xavier Pic</name></author><author><name sortKey="Arroyo, Juan" sort="Arroyo, Juan" uniqKey="Arroyo J" first="Juan" last="Arroyo">Juan Arroyo</name></author></analytic><series><title level="j">American journal of botany</title><idno type="ISSN">0002-9122</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>UNLABELLED</b></p><p></p></div><div type="abstract" xml:lang="en"><p><b>PREMISE OF THE STUDY</b></p><p>Microsatellite loci from a genomic library of the species Narcissus papyraceus were optimized and characterized for studies of population genetics. •</p></div><div type="abstract" xml:lang="en"><p><b>METHODS AND RESULTS</b></p><p>Eleven markers that were successfully amplified showed polymorphism when tested on 50 individuals from two populations in southern Spain and northern Morocco. Overall, the number of alleles per locus ranged between 4 and 15. Between 8 and 11 loci successfully amplified in other eight Narcissus species. •</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>These markers will enable genetic diversity studies of N. papyraceus across its distribution range and conduct paternity analyses among individuals differing in flower morphology.</p></div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">21622397</PMID><DateCompleted><Year>2012</Year><Month>10</Month><Day>02</Day></DateCompleted><DateRevised><Year>2014</Year><Month>07</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0002-9122</ISSN><JournalIssue CitedMedium="Print"><Volume>97</Volume><Issue>3</Issue><PubDate><Year>2010</Year><Month>Mar</Month></PubDate></JournalIssue><Title>American journal of botany</Title><ISOAbbreviation>Am J Bot</ISOAbbreviation></Journal><ArticleTitle>New microsatellite loci for Narcissus papyraceus (Amarillydaceae) and cross-amplification in other congeneric species.</ArticleTitle><Pagination><MedlinePgn>e10-3</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3732/ajb.1000023</ELocationID><Abstract><AbstractText Label="UNLABELLED"></AbstractText><AbstractText Label="PREMISE OF THE STUDY" NlmCategory="OBJECTIVE">Microsatellite loci from a genomic library of the species Narcissus papyraceus were optimized and characterized for studies of population genetics. •</AbstractText><AbstractText Label="METHODS AND RESULTS" NlmCategory="RESULTS">Eleven markers that were successfully amplified showed polymorphism when tested on 50 individuals from two populations in southern Spain and northern Morocco. Overall, the number of alleles per locus ranged between 4 and 15. Between 8 and 11 loci successfully amplified in other eight Narcissus species. •</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">These markers will enable genetic diversity studies of N. papyraceus across its distribution range and conduct paternity analyses among individuals differing in flower morphology.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Simón</LastName><ForeName>Violeta I</ForeName><Initials>VI</Initials><AffiliationInfo><Affiliation>Departamento de Biología Vegetal y Ecología, Universidad de Sevilla, Sevilla, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Picó</LastName><ForeName>F Xavier</ForeName><Initials>FX</Initials></Author><Author ValidYN="Y"><LastName>Arroyo</LastName><ForeName>Juan</ForeName><Initials>J</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>02</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Am J Bot</MedlineTA><NlmUniqueID>0370467</NlmUniqueID><ISSNLinking>0002-9122</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>5</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>3</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>3</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21622397</ArticleId><ArticleId IdType="pii">ajb.1000023</ArticleId><ArticleId IdType="doi">10.3732/ajb.1000023</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Espagne</li></country></list><tree><noCountry><name sortKey="Arroyo, Juan" sort="Arroyo, Juan" uniqKey="Arroyo J" first="Juan" last="Arroyo">Juan Arroyo</name><name sortKey="Pic, F Xavier" sort="Pic, F Xavier" uniqKey="Pic F" first="F Xavier" last="Pic">F Xavier Pic</name></noCountry><country name="Espagne"><noRegion><name sortKey="Sim N, Violeta I" sort="Sim N, Violeta I" uniqKey="Sim N V" first="Violeta I" last="Sim N">Violeta I. 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