MaghrebDataLibMedV2, Main, Exploration, bibRecord, 000466

The grapevine VvWRKY2 gene enhances salt and osmotic stress tolerance in transgenic Nicotiana tabacum

Identifieur interne : 000466 ( Main/Exploration ); précédent : 000465; suivant : 000467

The grapevine VvWRKY2 gene enhances salt and osmotic stress tolerance in transgenic Nicotiana tabacum

Auteurs : Rim Mzid [Tunisie] ; Walid Zorrig [Tunisie] ; Rayda Ben Ayed [Tunisie] ; Karim Ben Hamed [Tunisie] ; Mariem Ayadi [Tunisie] ; Yosra Damak [Tunisie] ; Virginie Lauvergeat [France] ; Mohsen Hanana [Tunisie]

Source :

3 Biotech [ 2190-572X ] ; 2018.

RBID : PMC:5972082

Abstract

Our study aims to assess the implication of WRKY transcription factor in the molecular mechanisms of grapevine adaptation to salt and water stresses. In this respect, a full-length VvWRKY2 cDNA, isolated from a Vitis vinifera grape berry cDNA library, was constitutively over-expressed in Nicotiana tabacum seedlings. Our results showed that transgenic tobacco plants exhibited higher seed germination rates and better growth, under both salt and osmotic stress treatments, when compared to wild type plants. Furthermore, our analyses demonstrated that, under stress conditions, transgenic plants accumulated more osmolytes, such as soluble sugars and free proline, while no changes were observed regarding electrolyte leakage, H₂O₂, and malondialdehyde contents. The improvement of osmotic adjustment may be an important mechanism underlying the role of VvWRKY2 in promoting tolerance and adaptation to abiotic stresses. Principal component analysis of our results highlighted a clear partition of plant response to stress. On the other hand, we observed a significant adaptation behaviour response for transgenic lines under stress. Taken together, all our findings suggest that over-expression of VvWRKY2 gene has a compelling role in abiotic stress tolerance and, therefore, would provide a useful strategy to promote abiotic stress tolerance in grape via molecular-assisted breeding and/or new biotechnology tools.

Electronic supplementary material

The online version of this article (10.1007/s13205-018-1301-4) contains supplementary material, which is available to authorized users.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972082

DOI: 10.1007/s13205-018-1301-4
PubMed: 29872608
PubMed Central: 5972082

Affiliations:

France, Tunisie

Le document en format XML

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 enhances salt and osmotic stress tolerance in transgenic <italic>Nicotiana tabacum</italic>
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<front><div type="abstract" xml:lang="en"><p>Our study aims to assess the implication of WRKY transcription factor in the molecular mechanisms of grapevine adaptation to salt and water stresses. In this respect, a full-length <italic>VvWRKY2</italic>
 cDNA, isolated from a <italic>Vitis vinifera</italic>
 grape berry cDNA library, was constitutively over-expressed in <italic>Nicotiana tabacum</italic>
 seedlings. Our results showed that transgenic tobacco plants exhibited higher seed germination rates and better growth, under both salt and osmotic stress treatments, when compared to wild type plants. Furthermore, our analyses demonstrated that, under stress conditions, transgenic plants accumulated more osmolytes, such as soluble sugars and free proline, while no changes were observed regarding electrolyte leakage, H<sub>2</sub>
O<sub>2</sub>
, and malondialdehyde contents. The improvement of osmotic adjustment may be an important mechanism underlying the role of <italic>VvWRKY</italic>
2 in promoting tolerance and adaptation to abiotic stresses. Principal component analysis of our results highlighted a clear partition of plant response to stress. On the other hand, we observed a significant adaptation behaviour response for transgenic lines under stress. Taken together, all our findings suggest that over-expression of <italic>VvWRKY2</italic>
 gene has a compelling role in abiotic stress tolerance and, therefore, would provide a useful strategy to promote abiotic stress tolerance in grape <italic>via</italic>
 molecular-assisted breeding and/or new biotechnology tools.</p>
<sec><title>Electronic supplementary material</title>
<p>The online version of this article (10.1007/s13205-018-1301-4) contains supplementary material, which is available to authorized users.</p>
</sec>
</div>
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The grapevine VvWRKY2 gene enhances salt and osmotic stress tolerance in transgenic Nicotiana tabacum

The grapevine VvWRKY2 gene enhances salt and osmotic stress tolerance in transgenic Nicotiana tabacum

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