Alkaloids Profiling of Fumaria capreolata by Analytical Platforms Based on the Hyphenation of Gas Chromatography and Liquid Chromatography with Quadrupole-Time-of-Flight Mass Spectrometry
Identifieur interne : 000432 ( Main/Exploration ); précédent : 000431; suivant : 000433Alkaloids Profiling of Fumaria capreolata by Analytical Platforms Based on the Hyphenation of Gas Chromatography and Liquid Chromatography with Quadrupole-Time-of-Flight Mass Spectrometry
Auteurs : María Del Mar Contreras [Espagne] ; Noureddine Bribi [Espagne, Algérie] ; Ana María G Mez-Caravaca [Espagne] ; Julio Gálvez [Espagne] ; Antonio Segura-Carretero [Espagne]Source :
- International Journal of Analytical Chemistry [ 1687-8760 ] ; 2017.
Abstract
Two analytical platforms, gas chromatography (GC) coupled to quadrupole-time-of-flight (QTOF) mass spectrometry (MS) and reversed-phase ultrahigh performance liquid chromatography (UHPLC) coupled to diode array (DAD) and QTOF detection, were applied in order to study the alkaloid profile of
Url:
DOI: 10.1155/2017/5178729
PubMed: 29348751
PubMed Central: 5733964
Affiliations:
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Fuentenueva s/n, 18071 Granada, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avda. Fuentenueva s/n, 18071 Granada</wicri:regionArea></affiliation></author><author><name sortKey="Bribi, Noureddine" sort="Bribi, Noureddine" uniqKey="Bribi N" first="Noureddine" last="Bribi">Noureddine Bribi</name><affiliation wicri:level="1"><nlm:aff id="I3">CIBER-EHD, Department of Pharmacology, ibs.GRANADA, Center for Biomedical Research (CIBM), University of Granada, Avenida del Conocimiento s/n, Armilla, 18016 Granada, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>CIBER-EHD, Department of Pharmacology, ibs.GRANADA, Center for Biomedical Research (CIBM), University of Granada, Avenida del Conocimiento s/n, Armilla, 18016 Granada</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="I4">Laboratoire de Biotechnologies Végétales et Ethnobotanique, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algeria</nlm:aff><country xml:lang="fr">Algérie</country><wicri:regionArea>Laboratoire de Biotechnologies Végétales et Ethnobotanique, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia</wicri:regionArea></affiliation></author><author><name sortKey="G Mez Caravaca, Ana Maria" sort="G Mez Caravaca, Ana Maria" uniqKey="G Mez Caravaca A" first="Ana María" last="G Mez-Caravaca">Ana María G Mez-Caravaca</name><affiliation wicri:level="1"><nlm:aff id="I2">Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avda. Fuentenueva s/n, 18071 Granada, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avda. Fuentenueva s/n, 18071 Granada</wicri:regionArea></affiliation></author><author><name sortKey="Galvez, Julio" sort="Galvez, Julio" uniqKey="Galvez J" first="Julio" last="Gálvez">Julio Gálvez</name><affiliation wicri:level="1"><nlm:aff id="I3">CIBER-EHD, Department of Pharmacology, ibs.GRANADA, Center for Biomedical Research (CIBM), University of Granada, Avenida del Conocimiento s/n, Armilla, 18016 Granada, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>CIBER-EHD, Department of Pharmacology, ibs.GRANADA, Center for Biomedical Research (CIBM), University of Granada, Avenida del Conocimiento s/n, Armilla, 18016 Granada</wicri:regionArea></affiliation></author><author><name sortKey="Segura Carretero, Antonio" sort="Segura Carretero, Antonio" uniqKey="Segura Carretero A" first="Antonio" last="Segura-Carretero">Antonio Segura-Carretero</name><affiliation wicri:level="1"><nlm:aff id="I1">Research and Development Functional Food Centre (CIDAF), Bioregión Building, Health Science Technological Park, Avenida del Conocimiento s/n, 18016 Granada, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Research and Development Functional Food Centre (CIDAF), Bioregión Building, Health Science Technological Park, Avenida del Conocimiento s/n, 18016 Granada</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="I2">Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avda. Fuentenueva s/n, 18071 Granada, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avda. Fuentenueva s/n, 18071 Granada</wicri:regionArea></affiliation></author></analytic><series><title level="j">International Journal of Analytical Chemistry</title><idno type="ISSN">1687-8760</idno><idno type="eISSN">1687-8779</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Two analytical platforms, gas chromatography (GC) coupled to quadrupole-time-of-flight (QTOF) mass spectrometry (MS) and reversed-phase ultrahigh performance liquid chromatography (UHPLC) coupled to diode array (DAD) and QTOF detection, were applied in order to study the alkaloid profile of<italic> Fumaria capreolata</italic>. The use of these mass analyzers enabled tentatively identifying the alkaloids by matching their accurate mass signals and suggested molecular formulae with those previously reported in libraries and databases. Moreover, the proposed structures were corroborated by studying their fragmentation pattern obtained by both platforms. In this way, 8 and 26 isoquinoline alkaloids were characterized using GC-QTOF-MS and RP-UHPLC-DAD-QTOF-MS, respectively, and they belonged to the following subclasses: protoberberine, protopine, aporphine, benzophenanthridine, spirobenzylisoquinoline, morphinandienone, and benzylisoquinoline. Moreover, the latter analytical method was selected to determine at 280 nm the concentration of protopine (9.6 ± 0.7 mg/g), a potential active compound of the extract. In conclusion, although GC-MS has been commonly used for the analysis of this type of phytochemicals, RP-UHPLC-DAD-QTOF-MS provided essential complementary information. This analytical method can be applied for the quality control of phytopharmaceuticals containing<italic> Fumaria</italic> extracts currently found in the market.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Asensi, M" uniqKey="Asensi M">M. Asensi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bribi, N" uniqKey="Bribi N">N. Bribi</name></author><author><name sortKey="Bouguezza, Y" uniqKey="Bouguezza Y">Y. Bouguezza</name></author><author><name sortKey="Maiza Benabdesselam, F" uniqKey="Maiza Benabdesselam F">F. Maiza-Benabdesselam</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jameel, M" uniqKey="Jameel M">M. Jameel</name></author><author><name sortKey="Ali, A" uniqKey="Ali A">A. Ali</name></author><author><name sortKey="Ali, M" uniqKey="Ali M">M. 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Pan</name></author></analytic></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Algérie</li><li>Espagne</li></country></list><tree><country name="Espagne"><noRegion><name sortKey="Contreras, Maria Del Mar" sort="Contreras, Maria Del Mar" uniqKey="Contreras M" first="María Del Mar" last="Contreras">María Del Mar Contreras</name></noRegion><name sortKey="Bribi, Noureddine" sort="Bribi, Noureddine" uniqKey="Bribi N" first="Noureddine" last="Bribi">Noureddine Bribi</name><name sortKey="Contreras, Maria Del Mar" sort="Contreras, Maria Del Mar" uniqKey="Contreras M" first="María Del Mar" last="Contreras">María Del Mar Contreras</name><name sortKey="G Mez Caravaca, Ana Maria" sort="G Mez Caravaca, Ana Maria" uniqKey="G Mez Caravaca A" first="Ana María" last="G Mez-Caravaca">Ana María G Mez-Caravaca</name><name sortKey="Galvez, Julio" sort="Galvez, Julio" uniqKey="Galvez J" first="Julio" last="Gálvez">Julio Gálvez</name><name sortKey="Segura Carretero, Antonio" sort="Segura Carretero, Antonio" uniqKey="Segura Carretero A" first="Antonio" last="Segura-Carretero">Antonio Segura-Carretero</name><name sortKey="Segura Carretero, Antonio" sort="Segura Carretero, Antonio" uniqKey="Segura Carretero A" first="Antonio" last="Segura-Carretero">Antonio Segura-Carretero</name></country><country name="Algérie"><noRegion><name sortKey="Bribi, Noureddine" sort="Bribi, Noureddine" uniqKey="Bribi N" first="Noureddine" last="Bribi">Noureddine Bribi</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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