Proteomics of the milk fat globule membrane from Camelus dromedarius.
Identifieur interne : 000614 ( Main/Exploration ); précédent : 000613; suivant : 000615Proteomics of the milk fat globule membrane from Camelus dromedarius.
Auteurs : Besma Saadaoui [Tunisie] ; Céline Henry ; Touhami Khorchani ; Mohamed Mars ; Patrice Martin ; Christelle CeboSource :
- Proteomics [ 1615-9861 ] ; 2013.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Annotation de séquence moléculaire (MeSH), Chameaux (métabolisme), Compartimentation cellulaire (MeSH), Glycolipides (métabolisme), Glycoprotéines (métabolisme), Gouttelettes lipidiques (MeSH), Membranes (MeSH), Protéines membranaires (métabolisme), Protéomique (méthodes), Électrophorèse sur gel de polyacrylamide (MeSH).
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Glycolipids, Glycoproteins, Membrane Proteins.
- metabolism : Camelus.
- methods : Proteomics.
- Animals, Cell Compartmentation, Electrophoresis, Polyacrylamide Gel, Lipid Droplets, Membranes, Molecular Sequence Annotation.
Abstract
Camel milk has been widely characterized with regards to casein and whey proteins. However, in camelids, almost nothing is known about the milk fat globule membrane (MFGM), the membrane surrounding fat globules in milk. The purpose of this study was thus to identify MFGM proteins from Camelus dromedarius milk. Major MFGM proteins (namely, fatty acid synthase, xanthine oxidase, butyrophilin, lactadherin, and adipophilin) already evidenced in cow milk were identified in camel milk using MS. In addition, a 1D-LC-MS/MS approach led us to identify 322 functional groups of proteins associated with the camel MFGM. Dromedary MFGM proteins were then classified into functional categories using DAVID (the Database for Annotation, Visualization, and Integrated Discovery) bioinformatics resources. More than 50% of MFGM proteins from camel milk were found to be integral membrane proteins (mostly belonging to the plasma membrane), or proteins associated to the membrane. Enriched GO terms associated with MFGM proteins from camel milk were protein transport (p-value = 1.73 × 10(-14)), translation (p-value = 1.08 × 10(-11)), lipid biosynthetic process (p-value = 6.72 × 10(-10)), hexose metabolic process (p-value = 1.89 × 10(-04)), and actin cytoskeleton organization (p-value = 2.72 × 10(-04)). These findings will help to contribute to a better characterization of camel milk. Identified MFGM proteins from camel milk may also provide new insight into lipid droplet formation in the mammary epithelial cell.
DOI: 10.1002/pmic.201200113
PubMed: 23349047
Affiliations:
Links toward previous steps (curation, corpus...)
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Patrice" sort="Martin, Patrice" uniqKey="Martin P" first="Patrice" last="Martin">Patrice Martin</name></author><author><name sortKey="Cebo, Christelle" sort="Cebo, Christelle" uniqKey="Cebo C" first="Christelle" last="Cebo">Christelle Cebo</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23349047</idno><idno type="pmid">23349047</idno><idno type="doi">10.1002/pmic.201200113</idno><idno type="wicri:Area/Main/Corpus">000634</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000634</idno><idno type="wicri:Area/Main/Curation">000634</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000634</idno><idno type="wicri:Area/Main/Exploration">000634</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Proteomics of the milk fat globule membrane from Camelus dromedarius.</title><author><name sortKey="Saadaoui, Besma" sort="Saadaoui, Besma" uniqKey="Saadaoui B" first="Besma" last="Saadaoui">Besma Saadaoui</name><affiliation wicri:level="1"><nlm:affiliation>Faculté des Sciences de Gabès cité Erriadh Zrig, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Faculté des Sciences de Gabès cité Erriadh Zrig</wicri:regionArea></affiliation></author><author><name sortKey="Henry, Celine" sort="Henry, Celine" uniqKey="Henry C" first="Céline" last="Henry">Céline Henry</name></author><author><name sortKey="Khorchani, Touhami" sort="Khorchani, Touhami" uniqKey="Khorchani T" first="Touhami" last="Khorchani">Touhami Khorchani</name></author><author><name sortKey="Mars, Mohamed" sort="Mars, Mohamed" uniqKey="Mars M" first="Mohamed" last="Mars">Mohamed Mars</name></author><author><name sortKey="Martin, Patrice" sort="Martin, Patrice" uniqKey="Martin P" first="Patrice" last="Martin">Patrice Martin</name></author><author><name sortKey="Cebo, Christelle" sort="Cebo, Christelle" uniqKey="Cebo C" first="Christelle" last="Cebo">Christelle Cebo</name></author></analytic><series><title level="j">Proteomics</title><idno type="eISSN">1615-9861</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Camelus (metabolism)</term><term>Cell Compartmentation (MeSH)</term><term>Electrophoresis, Polyacrylamide Gel (MeSH)</term><term>Glycolipids (metabolism)</term><term>Glycoproteins (metabolism)</term><term>Lipid Droplets (MeSH)</term><term>Membrane Proteins (metabolism)</term><term>Membranes (MeSH)</term><term>Molecular Sequence Annotation (MeSH)</term><term>Proteomics (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Annotation de séquence moléculaire (MeSH)</term><term>Chameaux (métabolisme)</term><term>Compartimentation cellulaire (MeSH)</term><term>Glycolipides (métabolisme)</term><term>Glycoprotéines (métabolisme)</term><term>Gouttelettes lipidiques (MeSH)</term><term>Membranes (MeSH)</term><term>Protéines membranaires (métabolisme)</term><term>Protéomique (méthodes)</term><term>Électrophorèse sur gel de polyacrylamide (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glycolipids</term><term>Glycoproteins</term><term>Membrane Proteins</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Camelus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Proteomics</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Chameaux</term><term>Glycolipides</term><term>Glycoprotéines</term><term>Protéines membranaires</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Protéomique</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Compartmentation</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Lipid Droplets</term><term>Membranes</term><term>Molecular Sequence Annotation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Annotation de séquence moléculaire</term><term>Compartimentation cellulaire</term><term>Gouttelettes lipidiques</term><term>Membranes</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Camel milk has been widely characterized with regards to casein and whey proteins. However, in camelids, almost nothing is known about the milk fat globule membrane (MFGM), the membrane surrounding fat globules in milk. The purpose of this study was thus to identify MFGM proteins from Camelus dromedarius milk. Major MFGM proteins (namely, fatty acid synthase, xanthine oxidase, butyrophilin, lactadherin, and adipophilin) already evidenced in cow milk were identified in camel milk using MS. In addition, a 1D-LC-MS/MS approach led us to identify 322 functional groups of proteins associated with the camel MFGM. Dromedary MFGM proteins were then classified into functional categories using DAVID (the Database for Annotation, Visualization, and Integrated Discovery) bioinformatics resources. More than 50% of MFGM proteins from camel milk were found to be integral membrane proteins (mostly belonging to the plasma membrane), or proteins associated to the membrane. Enriched GO terms associated with MFGM proteins from camel milk were protein transport (p-value = 1.73 × 10(-14)), translation (p-value = 1.08 × 10(-11)), lipid biosynthetic process (p-value = 6.72 × 10(-10)), hexose metabolic process (p-value = 1.89 × 10(-04)), and actin cytoskeleton organization (p-value = 2.72 × 10(-04)). These findings will help to contribute to a better characterization of camel milk. Identified MFGM proteins from camel milk may also provide new insight into lipid droplet formation in the mammary epithelial cell.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23349047</PMID><DateCompleted><Year>2013</Year><Month>09</Month><Day>20</Day></DateCompleted><DateRevised><Year>2020</Year><Month>12</Month><Day>09</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1615-9861</ISSN><JournalIssue CitedMedium="Internet"><Volume>13</Volume><Issue>7</Issue><PubDate><Year>2013</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Proteomics</Title><ISOAbbreviation>Proteomics</ISOAbbreviation></Journal><ArticleTitle>Proteomics of the milk fat globule membrane from Camelus dromedarius.</ArticleTitle><Pagination><MedlinePgn>1180-4</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/pmic.201200113</ELocationID><Abstract><AbstractText>Camel milk has been widely characterized with regards to casein and whey proteins. However, in camelids, almost nothing is known about the milk fat globule membrane (MFGM), the membrane surrounding fat globules in milk. The purpose of this study was thus to identify MFGM proteins from Camelus dromedarius milk. Major MFGM proteins (namely, fatty acid synthase, xanthine oxidase, butyrophilin, lactadherin, and adipophilin) already evidenced in cow milk were identified in camel milk using MS. In addition, a 1D-LC-MS/MS approach led us to identify 322 functional groups of proteins associated with the camel MFGM. Dromedary MFGM proteins were then classified into functional categories using DAVID (the Database for Annotation, Visualization, and Integrated Discovery) bioinformatics resources. More than 50% of MFGM proteins from camel milk were found to be integral membrane proteins (mostly belonging to the plasma membrane), or proteins associated to the membrane. Enriched GO terms associated with MFGM proteins from camel milk were protein transport (p-value = 1.73 × 10(-14)), translation (p-value = 1.08 × 10(-11)), lipid biosynthetic process (p-value = 6.72 × 10(-10)), hexose metabolic process (p-value = 1.89 × 10(-04)), and actin cytoskeleton organization (p-value = 2.72 × 10(-04)). These findings will help to contribute to a better characterization of camel milk. Identified MFGM proteins from camel milk may also provide new insight into lipid droplet formation in the mammary epithelial cell.</AbstractText><CopyrightInformation>© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Saadaoui</LastName><ForeName>Besma</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Faculté des Sciences de Gabès cité Erriadh Zrig, Tunisia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Henry</LastName><ForeName>Céline</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Khorchani</LastName><ForeName>Touhami</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Mars</LastName><ForeName>Mohamed</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Martin</LastName><ForeName>Patrice</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Cebo</LastName><ForeName>Christelle</ForeName><Initials>C</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>02</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Proteomics</MedlineTA><NlmUniqueID>101092707</NlmUniqueID><ISSNLinking>1615-9853</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006017">Glycolipids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006023">Glycoproteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008565">Membrane Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C110367">milk fat globule</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002162" 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PubStatus="pubmed"><Year>2013</Year><Month>1</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>9</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23349047</ArticleId><ArticleId IdType="doi">10.1002/pmic.201200113</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Tunisie</li></country></list><tree><noCountry><name sortKey="Cebo, Christelle" sort="Cebo, Christelle" uniqKey="Cebo C" first="Christelle" last="Cebo">Christelle Cebo</name><name sortKey="Henry, Celine" sort="Henry, Celine" uniqKey="Henry C" first="Céline" last="Henry">Céline Henry</name><name sortKey="Khorchani, Touhami" sort="Khorchani, Touhami" uniqKey="Khorchani T" first="Touhami" last="Khorchani">Touhami Khorchani</name><name sortKey="Mars, Mohamed" sort="Mars, Mohamed" uniqKey="Mars M" first="Mohamed" last="Mars">Mohamed Mars</name><name sortKey="Martin, Patrice" sort="Martin, Patrice" uniqKey="Martin P" first="Patrice" last="Martin">Patrice Martin</name></noCountry><country name="Tunisie"><noRegion><name sortKey="Saadaoui, Besma" sort="Saadaoui, Besma" uniqKey="Saadaoui B" first="Besma" last="Saadaoui">Besma Saadaoui</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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