Development of analytical methods GC-MS vs LC-UV for the serum monitoring of an inflammatory glycotoxin (methylglyoxal): A new biomarker of bovine hepatobiliary distomatosis.
Identifieur interne : 000203 ( Main/Corpus ); précédent : 000202; suivant : 000204Development of analytical methods GC-MS vs LC-UV for the serum monitoring of an inflammatory glycotoxin (methylglyoxal): A new biomarker of bovine hepatobiliary distomatosis.
Auteurs : Nadia Taïbi ; Amina Taïbi ; Rachid Ameraoui ; Mohamed Abou-Mustapha ; Mohamed Hadjadj ; Zahra-Mouna Boutaiba ; Amel Kaced ; Souhila Djema ; Qosay-Ali Al-Balas ; Ghazi-Ahmad Al Jabal ; Miriem Aissi ; Khaled Harhoura ; Safia Zenia ; Farida KhammarSource :
- Biochimie [ 1638-6183 ] ; 2020.
English descriptors
- KwdEn :
- MESH :
- chemical , blood : Biomarkers, Pyruvaldehyde.
- diagnosis : Fascioliasis.
- isolation & purification : Fasciola hepatica.
- methods : Chromatography, High Pressure Liquid, Gas Chromatography-Mass Spectrometry.
- veterinary : Fascioliasis.
- Animals, Cattle, Female, Male.
Abstract
Two analytical methods; high performance liquid chromatography and gas chromatography were used to determine the content of 2-methylquinoxaline, a methylglyoxal-derived agent in sera from cattle with fascioliasis. Methylglyoxal is a highly mutagenic and cytotoxic reactive dicarbonyl compound formed by non-enzymatic fragmentation of triose phosphate GAP and DHAP during glycolysis which regularly contributes to repositioning the energetic balance between physiological and pathological situations. The aim of this study was to propose the MGO as a new biomarker in the bovine fasciolosis. Strongly infected animals showed a correlation between the relatively high levels of Fasciola hepatica anti-f2 antibody and methylglyoxal compared to unharmed animals. Also, an acute hyperglycemia was recorded and closely related to hepatic parenchyma hyperplasia, inflammation, bile ducts obstruction and scléro-fibrous foci formation.Unlike HPLC, which has shown analytical flaws and irregularities, GC-MS remains an excellent diagnostic tool for detecting and quantifying methylglyoxal in biological fluids. The developed method has been validated under FDA guidelines. A full scan-range was set from m/z 39 to 144/999 and the molecular weight of the 2-methylquinoxaline was identified according to NIST Database and ES. Methylglyoxal was the only analyte successfully quantified in a relatively short run time. It was linear over a concentration range of 0.057-5.7 μg.ml-1with mean recoveries and RSD of 118% and 3.63% respectively. The intra and inter-day assays were satisfying and not exceed 3.00%. Results reflect the degree of precision of our method and indicate that MGO was an important contributor to understand the hepatic failure independently of other serum markers.
DOI: 10.1016/j.biochi.2019.11.002
PubMed: 31707099
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pubmed:31707099Le document en format XML
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Electronic address: Nadbio71.doc@gmail.com.</nlm:affiliation></affiliation></author><author><name sortKey="Taibi, Amina" sort="Taibi, Amina" uniqKey="Taibi A" first="Amina" last="Taïbi">Amina Taïbi</name><affiliation><nlm:affiliation>Laboratoire de Parasitologie et Mycologie, Laboratoire de Recherche Santé et Production Animale, École Nationale Supérieure Vétérinaire, B.P. 228, Rue Issad Abbes, El Alia, Oued Smar, Alger, Algeria. Electronic address: aminataibi.ensv@yahoo.com.</nlm:affiliation></affiliation></author><author><name sortKey="Ameraoui, Rachid" sort="Ameraoui, Rachid" uniqKey="Ameraoui R" first="Rachid" last="Ameraoui">Rachid Ameraoui</name><affiliation><nlm:affiliation>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza, Algeria. Electronic address: ame_rachid@yahoo.fr.</nlm:affiliation></affiliation></author><author><name sortKey="Abou Mustapha, Mohamed" sort="Abou Mustapha, Mohamed" uniqKey="Abou Mustapha M" first="Mohamed" last="Abou-Mustapha">Mohamed Abou-Mustapha</name><affiliation><nlm:affiliation>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza, Algeria. Electronic address: moho37@hotmail.fr.</nlm:affiliation></affiliation></author><author><name sortKey="Hadjadj, Mohamed" sort="Hadjadj, Mohamed" uniqKey="Hadjadj M" first="Mohamed" last="Hadjadj">Mohamed Hadjadj</name><affiliation><nlm:affiliation>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza, Algeria. Electronic address: mohhadj40@yahoo.fr.</nlm:affiliation></affiliation></author><author><name sortKey="Boutaiba, Zahra Mouna" sort="Boutaiba, Zahra Mouna" uniqKey="Boutaiba Z" first="Zahra-Mouna" last="Boutaiba">Zahra-Mouna Boutaiba</name><affiliation><nlm:affiliation>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza, Algeria. Electronic address: mounaboutaiba@yahoo.fr.</nlm:affiliation></affiliation></author><author><name sortKey="Kaced, Amel" sort="Kaced, Amel" uniqKey="Kaced A" first="Amel" last="Kaced">Amel Kaced</name><affiliation><nlm:affiliation>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza, Algeria. Electronic address: k.amel07@yahoo.fr.</nlm:affiliation></affiliation></author><author><name sortKey="Djema, Souhila" sort="Djema, Souhila" uniqKey="Djema S" first="Souhila" last="Djema">Souhila Djema</name><affiliation><nlm:affiliation>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza, Algeria. Electronic address: gensou85@hotmail.com.</nlm:affiliation></affiliation></author><author><name sortKey="Al Balas, Qosay Ali" sort="Al Balas, Qosay Ali" uniqKey="Al Balas Q" first="Qosay-Ali" last="Al-Balas">Qosay-Ali Al-Balas</name><affiliation><nlm:affiliation>Department of Medicinal Chemistry and Pharmacognosy Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan. Electronic address: qabalas@just.edu.jo.</nlm:affiliation></affiliation></author><author><name sortKey="Al Jabal, Ghazi Ahmad" sort="Al Jabal, Ghazi Ahmad" uniqKey="Al Jabal G" first="Ghazi-Ahmad" last="Al Jabal">Ghazi-Ahmad Al Jabal</name><affiliation><nlm:affiliation>Department of Medicinal Chemistry and Pharmacognosy Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan. Electronic address: gaaljabal12@ph.just.edu.jo.</nlm:affiliation></affiliation></author><author><name sortKey="Aissi, Miriem" sort="Aissi, Miriem" uniqKey="Aissi M" first="Miriem" last="Aissi">Miriem Aissi</name><affiliation><nlm:affiliation>Laboratoire de Parasitologie et Mycologie, Laboratoire de Recherche Santé et Production Animale, École Nationale Supérieure Vétérinaire, B.P. 228, Rue Issad Abbes, El Alia, Oued Smar, Alger, Algeria. Electronic address: aissimiriem@yahoo.fr.</nlm:affiliation></affiliation></author><author><name sortKey="Harhoura, Khaled" sort="Harhoura, Khaled" uniqKey="Harhoura K" first="Khaled" last="Harhoura">Khaled Harhoura</name><affiliation><nlm:affiliation>Laboratoire de Parasitologie et Mycologie, Laboratoire de Recherche Santé et Production Animale, École Nationale Supérieure Vétérinaire, B.P. 228, Rue Issad Abbes, El Alia, Oued Smar, Alger, Algeria. Electronic address: harhourakhaled@yahoo.fr.</nlm:affiliation></affiliation></author><author><name sortKey="Zenia, Safia" sort="Zenia, Safia" uniqKey="Zenia S" first="Safia" last="Zenia">Safia Zenia</name><affiliation><nlm:affiliation>Laboratoire de Parasitologie et Mycologie, Laboratoire de Recherche Santé et Production Animale, École Nationale Supérieure Vétérinaire, B.P. 228, Rue Issad Abbes, El Alia, Oued Smar, Alger, Algeria. Electronic address: safia_zenia@yahoo.fr.</nlm:affiliation></affiliation></author><author><name sortKey="Khammar, Farida" sort="Khammar, Farida" uniqKey="Khammar F" first="Farida" last="Khammar">Farida Khammar</name><affiliation><nlm:affiliation>Université des Sciences et de La Technologie Houari Boumediene (USTHB), Faculté des Sciences Biologiques (FSB), Laboratoire de Recherche sur Les Zones Arides, (LRZA), BP 32 El Alia 16111, Bab Ezzouar, 16111, Algeria. Electronic address: faridakhammar@gmail.com.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:31707099</idno><idno type="pmid">31707099</idno><idno type="doi">10.1016/j.biochi.2019.11.002</idno><idno type="wicri:Area/Main/Corpus">000203</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000203</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Development of analytical methods GC-MS vs LC-UV for the serum monitoring of an inflammatory glycotoxin (methylglyoxal): A new biomarker of bovine hepatobiliary distomatosis.</title><author><name sortKey="Taibi, Nadia" sort="Taibi, Nadia" uniqKey="Taibi N" first="Nadia" last="Taïbi">Nadia Taïbi</name><affiliation><nlm:affiliation>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza, Algeria; Université des Sciences et de La Technologie Houari Boumediene (USTHB), Faculté des Sciences Biologiques (FSB), Laboratoire de Recherche sur Les Zones Arides, (LRZA), BP 32 El Alia 16111, Bab Ezzouar, 16111, Algeria. Electronic address: Nadbio71.doc@gmail.com.</nlm:affiliation></affiliation></author><author><name sortKey="Taibi, Amina" sort="Taibi, Amina" uniqKey="Taibi A" first="Amina" last="Taïbi">Amina Taïbi</name><affiliation><nlm:affiliation>Laboratoire de Parasitologie et Mycologie, Laboratoire de Recherche Santé et Production Animale, École Nationale Supérieure Vétérinaire, B.P. 228, Rue Issad Abbes, El Alia, Oued Smar, Alger, Algeria. Electronic address: aminataibi.ensv@yahoo.com.</nlm:affiliation></affiliation></author><author><name sortKey="Ameraoui, Rachid" sort="Ameraoui, Rachid" uniqKey="Ameraoui R" first="Rachid" last="Ameraoui">Rachid Ameraoui</name><affiliation><nlm:affiliation>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza, Algeria. Electronic address: ame_rachid@yahoo.fr.</nlm:affiliation></affiliation></author><author><name sortKey="Abou Mustapha, Mohamed" sort="Abou Mustapha, Mohamed" uniqKey="Abou Mustapha M" first="Mohamed" last="Abou-Mustapha">Mohamed Abou-Mustapha</name><affiliation><nlm:affiliation>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza, Algeria. Electronic address: moho37@hotmail.fr.</nlm:affiliation></affiliation></author><author><name sortKey="Hadjadj, Mohamed" sort="Hadjadj, Mohamed" uniqKey="Hadjadj M" first="Mohamed" last="Hadjadj">Mohamed Hadjadj</name><affiliation><nlm:affiliation>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza, Algeria. Electronic address: mohhadj40@yahoo.fr.</nlm:affiliation></affiliation></author><author><name sortKey="Boutaiba, Zahra Mouna" sort="Boutaiba, Zahra Mouna" uniqKey="Boutaiba Z" first="Zahra-Mouna" last="Boutaiba">Zahra-Mouna Boutaiba</name><affiliation><nlm:affiliation>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza, Algeria. Electronic address: mounaboutaiba@yahoo.fr.</nlm:affiliation></affiliation></author><author><name sortKey="Kaced, Amel" sort="Kaced, Amel" uniqKey="Kaced A" first="Amel" last="Kaced">Amel Kaced</name><affiliation><nlm:affiliation>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza, Algeria. Electronic address: k.amel07@yahoo.fr.</nlm:affiliation></affiliation></author><author><name sortKey="Djema, Souhila" sort="Djema, Souhila" uniqKey="Djema S" first="Souhila" last="Djema">Souhila Djema</name><affiliation><nlm:affiliation>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza, Algeria. Electronic address: gensou85@hotmail.com.</nlm:affiliation></affiliation></author><author><name sortKey="Al Balas, Qosay Ali" sort="Al Balas, Qosay Ali" uniqKey="Al Balas Q" first="Qosay-Ali" last="Al-Balas">Qosay-Ali Al-Balas</name><affiliation><nlm:affiliation>Department of Medicinal Chemistry and Pharmacognosy Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan. Electronic address: qabalas@just.edu.jo.</nlm:affiliation></affiliation></author><author><name sortKey="Al Jabal, Ghazi Ahmad" sort="Al Jabal, Ghazi Ahmad" uniqKey="Al Jabal G" first="Ghazi-Ahmad" last="Al Jabal">Ghazi-Ahmad Al Jabal</name><affiliation><nlm:affiliation>Department of Medicinal Chemistry and Pharmacognosy Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan. Electronic address: gaaljabal12@ph.just.edu.jo.</nlm:affiliation></affiliation></author><author><name sortKey="Aissi, Miriem" sort="Aissi, Miriem" uniqKey="Aissi M" first="Miriem" last="Aissi">Miriem Aissi</name><affiliation><nlm:affiliation>Laboratoire de Parasitologie et Mycologie, Laboratoire de Recherche Santé et Production Animale, École Nationale Supérieure Vétérinaire, B.P. 228, Rue Issad Abbes, El Alia, Oued Smar, Alger, Algeria. Electronic address: aissimiriem@yahoo.fr.</nlm:affiliation></affiliation></author><author><name sortKey="Harhoura, Khaled" sort="Harhoura, Khaled" uniqKey="Harhoura K" first="Khaled" last="Harhoura">Khaled Harhoura</name><affiliation><nlm:affiliation>Laboratoire de Parasitologie et Mycologie, Laboratoire de Recherche Santé et Production Animale, École Nationale Supérieure Vétérinaire, B.P. 228, Rue Issad Abbes, El Alia, Oued Smar, Alger, Algeria. Electronic address: harhourakhaled@yahoo.fr.</nlm:affiliation></affiliation></author><author><name sortKey="Zenia, Safia" sort="Zenia, Safia" uniqKey="Zenia S" first="Safia" last="Zenia">Safia Zenia</name><affiliation><nlm:affiliation>Laboratoire de Parasitologie et Mycologie, Laboratoire de Recherche Santé et Production Animale, École Nationale Supérieure Vétérinaire, B.P. 228, Rue Issad Abbes, El Alia, Oued Smar, Alger, Algeria. Electronic address: safia_zenia@yahoo.fr.</nlm:affiliation></affiliation></author><author><name sortKey="Khammar, Farida" sort="Khammar, Farida" uniqKey="Khammar F" first="Farida" last="Khammar">Farida Khammar</name><affiliation><nlm:affiliation>Université des Sciences et de La Technologie Houari Boumediene (USTHB), Faculté des Sciences Biologiques (FSB), Laboratoire de Recherche sur Les Zones Arides, (LRZA), BP 32 El Alia 16111, Bab Ezzouar, 16111, Algeria. Electronic address: faridakhammar@gmail.com.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Biochimie</title><idno type="eISSN">1638-6183</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Biomarkers (blood)</term><term>Cattle (MeSH)</term><term>Chromatography, High Pressure Liquid (methods)</term><term>Fasciola hepatica (isolation & purification)</term><term>Fascioliasis (diagnosis)</term><term>Fascioliasis (veterinary)</term><term>Female (MeSH)</term><term>Gas Chromatography-Mass Spectrometry (methods)</term><term>Male (MeSH)</term><term>Pyruvaldehyde (blood)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Biomarkers</term><term>Pyruvaldehyde</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Fascioliasis</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Fasciola hepatica</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography, High Pressure Liquid</term><term>Gas Chromatography-Mass Spectrometry</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Fascioliasis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cattle</term><term>Female</term><term>Male</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Two analytical methods; high performance liquid chromatography and gas chromatography were used to determine the content of 2-methylquinoxaline, a methylglyoxal-derived agent in sera from cattle with fascioliasis. Methylglyoxal is a highly mutagenic and cytotoxic reactive dicarbonyl compound formed by non-enzymatic fragmentation of triose phosphate GAP and DHAP during glycolysis which regularly contributes to repositioning the energetic balance between physiological and pathological situations. The aim of this study was to propose the MGO as a new biomarker in the bovine fasciolosis. Strongly infected animals showed a correlation between the relatively high levels of Fasciola hepatica anti-f2 antibody and methylglyoxal compared to unharmed animals. Also, an acute hyperglycemia was recorded and closely related to hepatic parenchyma hyperplasia, inflammation, bile ducts obstruction and scléro-fibrous foci formation.Unlike HPLC, which has shown analytical flaws and irregularities, GC-MS remains an excellent diagnostic tool for detecting and quantifying methylglyoxal in biological fluids. The developed method has been validated under FDA guidelines. A full scan-range was set from m/z 39 to 144/999 and the molecular weight of the 2-methylquinoxaline was identified according to NIST Database and ES. Methylglyoxal was the only analyte successfully quantified in a relatively short run time. It was linear over a concentration range of 0.057-5.7 μg.ml-1with mean recoveries and RSD of 118% and 3.63% respectively. The intra and inter-day assays were satisfying and not exceed 3.00%. Results reflect the degree of precision of our method and indicate that MGO was an important contributor to understand the hepatic failure independently of other serum markers.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31707099</PMID><DateCompleted><Year>2020</Year><Month>04</Month><Day>06</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>08</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1638-6183</ISSN><JournalIssue CitedMedium="Internet"><Volume>168</Volume><PubDate><Year>2020</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Biochimie</Title><ISOAbbreviation>Biochimie</ISOAbbreviation></Journal><ArticleTitle>Development of analytical methods GC-MS vs LC-UV for the serum monitoring of an inflammatory glycotoxin (methylglyoxal): A new biomarker of bovine hepatobiliary distomatosis.</ArticleTitle><Pagination><MedlinePgn>169-184</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0300-9084(19)30317-7</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.biochi.2019.11.002</ELocationID><Abstract><AbstractText>Two analytical methods; high performance liquid chromatography and gas chromatography were used to determine the content of 2-methylquinoxaline, a methylglyoxal-derived agent in sera from cattle with fascioliasis. Methylglyoxal is a highly mutagenic and cytotoxic reactive dicarbonyl compound formed by non-enzymatic fragmentation of triose phosphate GAP and DHAP during glycolysis which regularly contributes to repositioning the energetic balance between physiological and pathological situations. The aim of this study was to propose the MGO as a new biomarker in the bovine fasciolosis. Strongly infected animals showed a correlation between the relatively high levels of Fasciola hepatica anti-f2 antibody and methylglyoxal compared to unharmed animals. Also, an acute hyperglycemia was recorded and closely related to hepatic parenchyma hyperplasia, inflammation, bile ducts obstruction and scléro-fibrous foci formation.Unlike HPLC, which has shown analytical flaws and irregularities, GC-MS remains an excellent diagnostic tool for detecting and quantifying methylglyoxal in biological fluids. The developed method has been validated under FDA guidelines. A full scan-range was set from m/z 39 to 144/999 and the molecular weight of the 2-methylquinoxaline was identified according to NIST Database and ES. Methylglyoxal was the only analyte successfully quantified in a relatively short run time. It was linear over a concentration range of 0.057-5.7 μg.ml-1with mean recoveries and RSD of 118% and 3.63% respectively. The intra and inter-day assays were satisfying and not exceed 3.00%. Results reflect the degree of precision of our method and indicate that MGO was an important contributor to understand the hepatic failure independently of other serum markers.</AbstractText><CopyrightInformation>Copyright © 2019 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Taïbi</LastName><ForeName>Nadia</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza, Algeria; Université des Sciences et de La Technologie Houari Boumediene (USTHB), Faculté des Sciences Biologiques (FSB), Laboratoire de Recherche sur Les Zones Arides, (LRZA), BP 32 El Alia 16111, Bab Ezzouar, 16111, Algeria. Electronic address: Nadbio71.doc@gmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Taïbi</LastName><ForeName>Amina</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Laboratoire de Parasitologie et Mycologie, Laboratoire de Recherche Santé et Production Animale, École Nationale Supérieure Vétérinaire, B.P. 228, Rue Issad Abbes, El Alia, Oued Smar, Alger, Algeria. Electronic address: aminataibi.ensv@yahoo.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ameraoui</LastName><ForeName>Rachid</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza, Algeria. Electronic address: ame_rachid@yahoo.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Abou-Mustapha</LastName><ForeName>Mohamed</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza, Algeria. Electronic address: moho37@hotmail.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hadjadj</LastName><ForeName>Mohamed</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza, Algeria. Electronic address: mohhadj40@yahoo.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Boutaiba</LastName><ForeName>Zahra-Mouna</ForeName><Initials>ZM</Initials><AffiliationInfo><Affiliation>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza, Algeria. Electronic address: mounaboutaiba@yahoo.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kaced</LastName><ForeName>Amel</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza, Algeria. Electronic address: k.amel07@yahoo.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Djema</LastName><ForeName>Souhila</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Centre de Recherche Scientifique et Technique en Analyses Physico-chimiques CRAPC, BP 384, Bou-Ismail, 42004, Tipaza, Algeria. Electronic address: gensou85@hotmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Al-Balas</LastName><ForeName>Qosay-Ali</ForeName><Initials>QA</Initials><AffiliationInfo><Affiliation>Department of Medicinal Chemistry and Pharmacognosy Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan. Electronic address: qabalas@just.edu.jo.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Al Jabal</LastName><ForeName>Ghazi-Ahmad</ForeName><Initials>GA</Initials><AffiliationInfo><Affiliation>Department of Medicinal Chemistry and Pharmacognosy Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan. Electronic address: gaaljabal12@ph.just.edu.jo.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Aissi</LastName><ForeName>Miriem</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Laboratoire de Parasitologie et Mycologie, Laboratoire de Recherche Santé et Production Animale, École Nationale Supérieure Vétérinaire, B.P. 228, Rue Issad Abbes, El Alia, Oued Smar, Alger, Algeria. Electronic address: aissimiriem@yahoo.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Harhoura</LastName><ForeName>Khaled</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Laboratoire de Parasitologie et Mycologie, Laboratoire de Recherche Santé et Production Animale, École Nationale Supérieure Vétérinaire, B.P. 228, Rue Issad Abbes, El Alia, Oued Smar, Alger, Algeria. Electronic address: harhourakhaled@yahoo.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zenia</LastName><ForeName>Safia</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Laboratoire de Parasitologie et Mycologie, Laboratoire de Recherche Santé et Production Animale, École Nationale Supérieure Vétérinaire, B.P. 228, Rue Issad Abbes, El Alia, Oued Smar, Alger, Algeria. Electronic address: safia_zenia@yahoo.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Khammar</LastName><ForeName>Farida</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Université des Sciences et de La Technologie Houari Boumediene (USTHB), Faculté des Sciences Biologiques (FSB), Laboratoire de Recherche sur Les Zones Arides, (LRZA), BP 32 El Alia 16111, Bab Ezzouar, 16111, Algeria. Electronic address: faridakhammar@gmail.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D023361">Validation Study</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>11</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>France</Country><MedlineTA>Biochimie</MedlineTA><NlmUniqueID>1264604</NlmUniqueID><ISSNLinking>0300-9084</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015415">Biomarkers</NameOfSubstance></Chemical><Chemical><RegistryNumber>722KLD7415</RegistryNumber><NameOfSubstance UI="D011765">Pyruvaldehyde</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015415" MajorTopicYN="N">Biomarkers</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002417" MajorTopicYN="N">Cattle</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002851" MajorTopicYN="N">Chromatography, High Pressure Liquid</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005210" MajorTopicYN="N">Fasciola hepatica</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005211" MajorTopicYN="N">Fascioliasis</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000662" MajorTopicYN="Y">veterinary</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008401" MajorTopicYN="N">Gas Chromatography-Mass Spectrometry</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011765" MajorTopicYN="N">Pyruvaldehyde</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Anti-f2 antibody of Fasciola hepatica</Keyword><Keyword MajorTopicYN="N">Cattle</Keyword><Keyword MajorTopicYN="N">GC-MS</Keyword><Keyword MajorTopicYN="N">Glucose</Keyword><Keyword MajorTopicYN="N">HPLC-UV</Keyword><Keyword MajorTopicYN="N">Methylglyoxal</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>08</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>11</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>11</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>4</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>11</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31707099</ArticleId><ArticleId IdType="pii">S0300-9084(19)30317-7</ArticleId><ArticleId IdType="doi">10.1016/j.biochi.2019.11.002</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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