Phosphorylcholine-bearing antigens in filarial nematode parasites: analysis of somatic extracts, in-vitro secretions and infection sera from Brugia malayi and B. pahangi.
Identifieur interne : 006486 ( PubMed/Curation ); précédent : 006485; suivant : 006487Phosphorylcholine-bearing antigens in filarial nematode parasites: analysis of somatic extracts, in-vitro secretions and infection sera from Brugia malayi and B. pahangi.
Auteurs : R M Maizels ; J. Burke ; D A DenhamSource :
- Parasite immunology [ 0141-9838 ] ; 1987.
Descripteurs français
- KwdFr :
- Animaux, Anticorps monoclonaux (immunologie), Antigènes d'helminthe (analyse), Antigènes d'helminthe (immunologie), Brugia (immunologie), Choline (analogues et dérivés), Chromatographie sur gel, Complexe antigène-anticorps, Dosage radioimmunologique, Femelle, Filariose lymphatique (immunologie), Gerbillinae, Haptènes (analyse), Haptènes (immunologie), Mâle, Phosphoryl-choline (analyse), Phosphoryl-choline (immunologie), Réactions croisées, Test ELISA, Électrophorèse sur gel de polyacrylamide, Épitopes (analyse), Épitopes (immunologie).
- MESH :
- analogues et dérivés : Choline.
- analyse : Antigènes d'helminthe, Haptènes, Phosphoryl-choline, Épitopes.
- immunologie : Anticorps monoclonaux, Antigènes d'helminthe, Brugia, Filariose lymphatique, Haptènes, Phosphoryl-choline, Épitopes.
- Animaux, Chromatographie sur gel, Complexe antigène-anticorps, Dosage radioimmunologique, Femelle, Gerbillinae, Mâle, Réactions croisées, Test ELISA, Électrophorèse sur gel de polyacrylamide.
English descriptors
- KwdEn :
- Animals, Antibodies, Monoclonal (immunology), Antigen-Antibody Complex, Antigens, Helminth (analysis), Antigens, Helminth (immunology), Brugia (immunology), Choline (analogs & derivatives), Chromatography, Gel, Cross Reactions, Electrophoresis, Polyacrylamide Gel, Elephantiasis, Filarial (immunology), Enzyme-Linked Immunosorbent Assay, Epitopes (analysis), Epitopes (immunology), Female, Gerbillinae, Haptens (analysis), Haptens (immunology), Male, Phosphorylcholine (analysis), Phosphorylcholine (immunology), Radioimmunoassay.
- MESH :
- chemical , analogs & derivatives : Choline.
- chemical , analysis : Antigens, Helminth, Epitopes, Haptens, Phosphorylcholine.
- chemical , immunology : Antibodies, Monoclonal, Antigens, Helminth, Epitopes, Haptens, Phosphorylcholine.
- immunology : Brugia, Elephantiasis, Filarial.
- Animals, Antigen-Antibody Complex, Chromatography, Gel, Cross Reactions, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Female, Gerbillinae, Male, Radioimmunoassay.
Abstract
A set of cross-reactive antigens is described which are present in somatic extracts and in-vitro secretions of the filarial nematodes Brugia pahangi and B. malayi. A monoclonal antibody reactive with a repeating epitope on these molecules readily detects circulating antigen in the serum of animals infected with lymphatic filariae, using either an immunoradiometric assay or enzyme-linked immunosorbent assay (ELISA). This epitope has the immunological reactivity and chemical characteristics of the phosphorylcholine (PC) hapten. The anti-PC monoclonal has been used to define the antigens bearing this epitope, and in chromatographic studies on material from extracts of Brugia adult worms, a heterogeneous profile of PC-positive molecules are found. In sera from Brugia-infected jirds, an antigen with a native molecular weight of approximately 500,000 is observed, which displays limited sensitivity to protease degradation. However, denatured samples on Western blots show a major parasite circulating antigen of Mr 90,000. The detection of this antigen in the presence of excess host antibody is also demonstrated, taking advantage of the stability of the target epitope to a range of treatments designed to dissociate and eliminate immune complexes.
PubMed: 2436131
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Burke</name></author><author><name sortKey="Denham, D A" sort="Denham, D A" uniqKey="Denham D" first="D A" last="Denham">D A Denham</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1987">1987</date><idno type="RBID">pubmed:2436131</idno><idno type="pmid">2436131</idno><idno type="wicri:Area/PubMed/Corpus">006486</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">006486</idno><idno type="wicri:Area/PubMed/Curation">006486</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">006486</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Phosphorylcholine-bearing antigens in filarial nematode parasites: analysis of somatic extracts, in-vitro secretions and infection sera from Brugia malayi and B. pahangi.</title><author><name sortKey="Maizels, R M" sort="Maizels, R M" uniqKey="Maizels R" first="R M" last="Maizels">R M Maizels</name></author><author><name sortKey="Burke, J" sort="Burke, J" uniqKey="Burke J" first="J" last="Burke">J. Burke</name></author><author><name sortKey="Denham, D A" sort="Denham, D A" uniqKey="Denham D" first="D A" last="Denham">D A Denham</name></author></analytic><series><title level="j">Parasite immunology</title><idno type="ISSN">0141-9838</idno><imprint><date when="1987" type="published">1987</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal (immunology)</term><term>Antigen-Antibody Complex</term><term>Antigens, Helminth (analysis)</term><term>Antigens, Helminth (immunology)</term><term>Brugia (immunology)</term><term>Choline (analogs & derivatives)</term><term>Chromatography, Gel</term><term>Cross Reactions</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Elephantiasis, Filarial (immunology)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Epitopes (analysis)</term><term>Epitopes (immunology)</term><term>Female</term><term>Gerbillinae</term><term>Haptens (analysis)</term><term>Haptens (immunology)</term><term>Male</term><term>Phosphorylcholine (analysis)</term><term>Phosphorylcholine (immunology)</term><term>Radioimmunoassay</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps monoclonaux (immunologie)</term><term>Antigènes d'helminthe (analyse)</term><term>Antigènes d'helminthe (immunologie)</term><term>Brugia (immunologie)</term><term>Choline (analogues et dérivés)</term><term>Chromatographie sur gel</term><term>Complexe antigène-anticorps</term><term>Dosage radioimmunologique</term><term>Femelle</term><term>Filariose lymphatique (immunologie)</term><term>Gerbillinae</term><term>Haptènes (analyse)</term><term>Haptènes (immunologie)</term><term>Mâle</term><term>Phosphoryl-choline (analyse)</term><term>Phosphoryl-choline (immunologie)</term><term>Réactions croisées</term><term>Test ELISA</term><term>Électrophorèse sur gel de polyacrylamide</term><term>Épitopes (analyse)</term><term>Épitopes (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Choline</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antigens, Helminth</term><term>Epitopes</term><term>Haptens</term><term>Phosphorylcholine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Antigens, Helminth</term><term>Epitopes</term><term>Haptens</term><term>Phosphorylcholine</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Choline</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Antigènes d'helminthe</term><term>Haptènes</term><term>Phosphoryl-choline</term><term>Épitopes</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps monoclonaux</term><term>Antigènes d'helminthe</term><term>Brugia</term><term>Filariose lymphatique</term><term>Haptènes</term><term>Phosphoryl-choline</term><term>Épitopes</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Brugia</term><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Antigen-Antibody Complex</term><term>Chromatography, Gel</term><term>Cross Reactions</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Female</term><term>Gerbillinae</term><term>Male</term><term>Radioimmunoassay</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Chromatographie sur gel</term><term>Complexe antigène-anticorps</term><term>Dosage radioimmunologique</term><term>Femelle</term><term>Gerbillinae</term><term>Mâle</term><term>Réactions croisées</term><term>Test ELISA</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A set of cross-reactive antigens is described which are present in somatic extracts and in-vitro secretions of the filarial nematodes Brugia pahangi and B. malayi. A monoclonal antibody reactive with a repeating epitope on these molecules readily detects circulating antigen in the serum of animals infected with lymphatic filariae, using either an immunoradiometric assay or enzyme-linked immunosorbent assay (ELISA). This epitope has the immunological reactivity and chemical characteristics of the phosphorylcholine (PC) hapten. The anti-PC monoclonal has been used to define the antigens bearing this epitope, and in chromatographic studies on material from extracts of Brugia adult worms, a heterogeneous profile of PC-positive molecules are found. In sera from Brugia-infected jirds, an antigen with a native molecular weight of approximately 500,000 is observed, which displays limited sensitivity to protease degradation. However, denatured samples on Western blots show a major parasite circulating antigen of Mr 90,000. The detection of this antigen in the presence of excess host antibody is also demonstrated, taking advantage of the stability of the target epitope to a range of treatments designed to dissociate and eliminate immune complexes.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">2436131</PMID><DateCreated><Year>1987</Year><Month>04</Month><Day>27</Day></DateCreated><DateCompleted><Year>1987</Year><Month>04</Month><Day>27</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0141-9838</ISSN><JournalIssue CitedMedium="Print"><Volume>9</Volume><Issue>1</Issue><PubDate><Year>1987</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Parasite immunology</Title><ISOAbbreviation>Parasite Immunol.</ISOAbbreviation></Journal><ArticleTitle>Phosphorylcholine-bearing antigens in filarial nematode parasites: analysis of somatic extracts, in-vitro secretions and infection sera from Brugia malayi and B. pahangi.</ArticleTitle><Pagination><MedlinePgn>49-66</MedlinePgn></Pagination><Abstract><AbstractText>A set of cross-reactive antigens is described which are present in somatic extracts and in-vitro secretions of the filarial nematodes Brugia pahangi and B. malayi. A monoclonal antibody reactive with a repeating epitope on these molecules readily detects circulating antigen in the serum of animals infected with lymphatic filariae, using either an immunoradiometric assay or enzyme-linked immunosorbent assay (ELISA). This epitope has the immunological reactivity and chemical characteristics of the phosphorylcholine (PC) hapten. The anti-PC monoclonal has been used to define the antigens bearing this epitope, and in chromatographic studies on material from extracts of Brugia adult worms, a heterogeneous profile of PC-positive molecules are found. In sera from Brugia-infected jirds, an antigen with a native molecular weight of approximately 500,000 is observed, which displays limited sensitivity to protease degradation. However, denatured samples on Western blots show a major parasite circulating antigen of Mr 90,000. The detection of this antigen in the presence of excess host antibody is also demonstrated, taking advantage of the stability of the target epitope to a range of treatments designed to dissociate and eliminate immune complexes.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Maizels</LastName><ForeName>R M</ForeName><Initials>RM</Initials></Author><Author ValidYN="Y"><LastName>Burke</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Denham</LastName><ForeName>D A</ForeName><Initials>DA</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Parasite 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UI="D002794">Choline</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000911" MajorTopicYN="N">Antibodies, Monoclonal</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000936" MajorTopicYN="N">Antigen-Antibody Complex</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000947" MajorTopicYN="N">Antigens, Helminth</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002009" MajorTopicYN="N">Brugia</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002794" MajorTopicYN="N">Choline</DescriptorName><QualifierName UI="Q000031" MajorTopicYN="Y">analogs & derivatives</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002850" MajorTopicYN="N">Chromatography, Gel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003429" MajorTopicYN="N">Cross Reactions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004591" MajorTopicYN="N">Electrophoresis, Polyacrylamide Gel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004605" MajorTopicYN="N">Elephantiasis, Filarial</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000939" MajorTopicYN="N">Epitopes</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005849" MajorTopicYN="N">Gerbillinae</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006241" MajorTopicYN="N">Haptens</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010767" MajorTopicYN="N">Phosphorylcholine</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011863" MajorTopicYN="N">Radioimmunoassay</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1987</Year><Month>1</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1987</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1987</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">2436131</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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