A highly specific and sensitive monoclonal antibody-based ELISA for the detection of circulating antigen in bancroftian filariasis.
Identifieur interne : 005C66 ( PubMed/Curation ); précédent : 005C65; suivant : 005C67A highly specific and sensitive monoclonal antibody-based ELISA for the detection of circulating antigen in bancroftian filariasis.
Auteurs : S J More [Australie] ; D B CopemanSource :
- Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ) [ 0177-2392 ] ; 1990.
Descripteurs français
- KwdFr :
- MESH :
- diagnostic : Filariose lymphatique.
- immunologie : Anticorps monoclonaux, Antigènes d'helminthe, Onchocerca, Wuchereria bancrofti.
- sang : Antigènes d'helminthe.
- Animaux, Femelle, Humains, Mâle, Test ELISA, Valeur prédictive des tests.
English descriptors
- KwdEn :
- MESH :
- chemical , blood : Antigens, Helminth.
- chemical , immunology : Antibodies, Monoclonal, Antigens, Helminth.
- diagnosis : Elephantiasis, Filarial.
- immunology : Onchocerca, Wuchereria bancrofti.
- Animals, Enzyme-Linked Immunosorbent Assay, Female, Humans, Male, Predictive Value of Tests.
Abstract
A monoclonal antibody, Og4C3, directed against antigens of Onchocerca gibsoni (but not phosphorylcholine) has been used in a sandwich ELISA to detect a circulating antigen of Wuchereria bancrofti in human serum. The interfering effect of host antibody was reduced by first boiling one part of serum for 5 min in the presence of three parts of 0.1 M Na2EDTA, pH 4.0. A total of 119 sera from individuals and 8 pooled sera from clinically and/or parasitologically defined cases of filariasis, plus 8 individual and 1 pooled endemic control sera, all from the filariasis serum bank of the World Health Organisation, as well as 20 non-endemic control sera, were screened with the assay. Circulating antigen was detected in serum from people infected with W. bancrofti but not Brugia malayi. B. timori, O. volvulus or Loa loa, and not in endemic or non-endemic controls. Of the 68 sera from W. bancrofti-infected subjects, 55/55 parasitologically confirmed and 12/13 clinically confirmed but amicrofilaraemic cases reacted in the assay. A weak but significant correlation (r2 = 0.4016) was found between numbers of microfilariae in blood and detectable levels of circulating antigen from patients with bancroftian filariasis.
PubMed: 2075384
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :005C66
Links to Exploration step
pubmed:2075384Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A highly specific and sensitive monoclonal antibody-based ELISA for the detection of circulating antigen in bancroftian filariasis.</title><author><name sortKey="More, S J" sort="More, S J" uniqKey="More S" first="S J" last="More">S J More</name><affiliation wicri:level="1"><nlm:affiliation>Graduate School of Tropical Veterinary Science and Agriculture, James Cook University of North Queensland, Townsville, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Graduate School of Tropical Veterinary Science and Agriculture, James Cook University of North Queensland, Townsville</wicri:regionArea></affiliation></author><author><name sortKey="Copeman, D B" sort="Copeman, D B" uniqKey="Copeman D" first="D B" last="Copeman">D B Copeman</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1990">1990</date><idno type="RBID">pubmed:2075384</idno><idno type="pmid">2075384</idno><idno type="wicri:Area/PubMed/Corpus">005C66</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">005C66</idno><idno type="wicri:Area/PubMed/Curation">005C66</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">005C66</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A highly specific and sensitive monoclonal antibody-based ELISA for the detection of circulating antigen in bancroftian filariasis.</title><author><name sortKey="More, S J" sort="More, S J" uniqKey="More S" first="S J" last="More">S J More</name><affiliation wicri:level="1"><nlm:affiliation>Graduate School of Tropical Veterinary Science and Agriculture, James Cook University of North Queensland, Townsville, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Graduate School of Tropical Veterinary Science and Agriculture, James Cook University of North Queensland, Townsville</wicri:regionArea></affiliation></author><author><name sortKey="Copeman, D B" sort="Copeman, D B" uniqKey="Copeman D" first="D B" last="Copeman">D B Copeman</name></author></analytic><series><title level="j">Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ)</title><idno type="ISSN">0177-2392</idno><imprint><date when="1990" type="published">1990</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal (immunology)</term><term>Antigens, Helminth (blood)</term><term>Antigens, Helminth (immunology)</term><term>Elephantiasis, Filarial (diagnosis)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Female</term><term>Humans</term><term>Male</term><term>Onchocerca (immunology)</term><term>Predictive Value of Tests</term><term>Wuchereria bancrofti (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps monoclonaux (immunologie)</term><term>Antigènes d'helminthe (immunologie)</term><term>Antigènes d'helminthe (sang)</term><term>Femelle</term><term>Filariose lymphatique (diagnostic)</term><term>Humains</term><term>Mâle</term><term>Onchocerca (immunologie)</term><term>Test ELISA</term><term>Valeur prédictive des tests</term><term>Wuchereria bancrofti (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antigens, Helminth</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Antigens, Helminth</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps monoclonaux</term><term>Antigènes d'helminthe</term><term>Onchocerca</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Onchocerca</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Antigènes d'helminthe</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Female</term><term>Humans</term><term>Male</term><term>Predictive Value of Tests</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Femelle</term><term>Humains</term><term>Mâle</term><term>Test ELISA</term><term>Valeur prédictive des tests</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A monoclonal antibody, Og4C3, directed against antigens of Onchocerca gibsoni (but not phosphorylcholine) has been used in a sandwich ELISA to detect a circulating antigen of Wuchereria bancrofti in human serum. The interfering effect of host antibody was reduced by first boiling one part of serum for 5 min in the presence of three parts of 0.1 M Na2EDTA, pH 4.0. A total of 119 sera from individuals and 8 pooled sera from clinically and/or parasitologically defined cases of filariasis, plus 8 individual and 1 pooled endemic control sera, all from the filariasis serum bank of the World Health Organisation, as well as 20 non-endemic control sera, were screened with the assay. Circulating antigen was detected in serum from people infected with W. bancrofti but not Brugia malayi. B. timori, O. volvulus or Loa loa, and not in endemic or non-endemic controls. Of the 68 sera from W. bancrofti-infected subjects, 55/55 parasitologically confirmed and 12/13 clinically confirmed but amicrofilaraemic cases reacted in the assay. A weak but significant correlation (r2 = 0.4016) was found between numbers of microfilariae in blood and detectable levels of circulating antigen from patients with bancroftian filariasis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">2075384</PMID><DateCreated><Year>1991</Year><Month>04</Month><Day>16</Day></DateCreated><DateCompleted><Year>1991</Year><Month>04</Month><Day>16</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0177-2392</ISSN><JournalIssue CitedMedium="Print"><Volume>41</Volume><Issue>4</Issue><PubDate><Year>1990</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ)</Title><ISOAbbreviation>Trop. Med. Parasitol.</ISOAbbreviation></Journal><ArticleTitle>A highly specific and sensitive monoclonal antibody-based ELISA for the detection of circulating antigen in bancroftian filariasis.</ArticleTitle><Pagination><MedlinePgn>403-6</MedlinePgn></Pagination><Abstract><AbstractText>A monoclonal antibody, Og4C3, directed against antigens of Onchocerca gibsoni (but not phosphorylcholine) has been used in a sandwich ELISA to detect a circulating antigen of Wuchereria bancrofti in human serum. The interfering effect of host antibody was reduced by first boiling one part of serum for 5 min in the presence of three parts of 0.1 M Na2EDTA, pH 4.0. A total of 119 sera from individuals and 8 pooled sera from clinically and/or parasitologically defined cases of filariasis, plus 8 individual and 1 pooled endemic control sera, all from the filariasis serum bank of the World Health Organisation, as well as 20 non-endemic control sera, were screened with the assay. Circulating antigen was detected in serum from people infected with W. bancrofti but not Brugia malayi. B. timori, O. volvulus or Loa loa, and not in endemic or non-endemic controls. Of the 68 sera from W. bancrofti-infected subjects, 55/55 parasitologically confirmed and 12/13 clinically confirmed but amicrofilaraemic cases reacted in the assay. A weak but significant correlation (r2 = 0.4016) was found between numbers of microfilariae in blood and detectable levels of circulating antigen from patients with bancroftian filariasis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>More</LastName><ForeName>S J</ForeName><Initials>SJ</Initials><AffiliationInfo><Affiliation>Graduate School of Tropical Veterinary Science and Agriculture, James Cook University of North Queensland, Townsville, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Copeman</LastName><ForeName>D B</ForeName><Initials>DB</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Trop Med Parasitol</MedlineTA><NlmUniqueID>8503728</NlmUniqueID><ISSNLinking>0177-2392</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000911">Antibodies, Monoclonal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000947">Antigens, Helminth</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000911" MajorTopicYN="Y">Antibodies, Monoclonal</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000947" MajorTopicYN="N">Antigens, Helminth</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004605" MajorTopicYN="N">Elephantiasis, Filarial</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="Y">Enzyme-Linked Immunosorbent Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009854" MajorTopicYN="N">Onchocerca</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011237" MajorTopicYN="N">Predictive Value of Tests</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014958" MajorTopicYN="N">Wuchereria bancrofti</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1990</Year><Month>12</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1990</Year><Month>12</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1990</Year><Month>12</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">2075384</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/PubMed/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 005C66 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd -nk 005C66 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= PubMed
|étape= Curation
|type= RBID
|clé= pubmed:2075384
|texte= A highly specific and sensitive monoclonal antibody-based ELISA for the detection of circulating antigen in bancroftian filariasis.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Curation/RBID.i -Sk "pubmed:2075384" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |