A Brugia malayi antigen specifically recognized by infected individuals.
Identifieur interne : 004E16 ( PubMed/Curation ); précédent : 004E15; suivant : 004E17A Brugia malayi antigen specifically recognized by infected individuals.
Auteurs : N. Rahmah [Malaisie] ; A K Anuar ; A N A'Shikin ; B H Lim ; R. Mehdi ; B. Abdullah ; M N ZuraineeSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 1998.
Descripteurs français
- KwdFr :
- Adulte, Animaux, Anticorps antihelminthe (immunologie), Anticorps antihelminthe (sang), Antigènes d'helminthe (immunologie), Antigènes d'helminthe (sang), Brugia malayi (immunologie), Dosage immunologique (), Filariose lymphatique (immunologie), Filariose lymphatique (sang), Humains, Immunoglobuline G (sang), Sensibilité et spécificité.
- MESH :
English descriptors
- KwdEn :
- Adult, Animals, Antibodies, Helminth (blood), Antibodies, Helminth (immunology), Antigens, Helminth (blood), Antigens, Helminth (immunology), Brugia malayi (immunology), Elephantiasis, Filarial (blood), Elephantiasis, Filarial (immunology), Humans, Immunoassay (methods), Immunoglobulin G (blood), Sensitivity and Specificity.
- MESH :
- chemical , blood : Antibodies, Helminth, Antigens, Helminth, Immunoglobulin G.
- chemical , immunology : Antibodies, Helminth, Antigens, Helminth.
- blood : Elephantiasis, Filarial.
- immunology : Brugia malayi, Elephantiasis, Filarial.
- methods : Immunoassay.
- Adult, Animals, Humans, Sensitivity and Specificity.
Abstract
Western blot analyses were performed on 444 serum specimens: 40 sera from microfilaraemic individuals, 10 sera from elephantiasis patients, 24 treated individuals, 50 sera from residents of endemic areas without anti-filarial IgG4 antibodies (endemic normals), 20 sera from amicrofilaraemic individuals with high anti-filarial IgG4 antibodies, 200 sera from healthy city-dwellers (non-endemic samples), and 100 sera from soil-transmitted helminth-infected individuals. Phast electrophoresis system was used to electrophorese Brugia malayi soluble adult worm antigen on 10-15% SDS-PAGE gradient gels followed by electrophoretic transfer onto PVDF membranes. Membrane strips were then successively incubated with blocking solution, human sera, and monoclonal anti-human IgG4 antibody-HRP, with adequate washings done in between each incubation step. Luminol chemiluminescence detection was then used to develop the blots. An antigenic band with the MW of approximately 37 kDa was found to be consistently present in the Western blots of all microfilaraemic sera, all amicrofilaraemic sera with high titres of anti-filarial IgG4 antibodies, some treated patients, and some elephantiasis patients. The antigen did not occur in immunoblots of individuals with other helminthic infections, normal endemic individuals, and city dwellers. Therefore the B. malayi antigen of with the MW of approximately 37 kDa demonstrated specific reactions with sera of B. malayi-infected individuals and thus may be useful for diagnostic application.
DOI: 10.1006/bbrc.1998.9360
PubMed: 9784388
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Rahmah</name><affiliation wicri:level="1"><nlm:affiliation>School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia. rahmah@kb.usm.my</nlm:affiliation><country xml:lang="fr">Malaisie</country><wicri:regionArea>School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan</wicri:regionArea></affiliation></author><author><name sortKey="Anuar, A K" sort="Anuar, A K" uniqKey="Anuar A" first="A K" last="Anuar">A K Anuar</name></author><author><name sortKey="A Shikin, A N" sort="A Shikin, A N" uniqKey="A Shikin A" first="A N" last="A'Shikin">A N A'Shikin</name></author><author><name sortKey="Lim, B H" sort="Lim, B H" uniqKey="Lim B" first="B H" last="Lim">B H Lim</name></author><author><name sortKey="Mehdi, R" sort="Mehdi, R" uniqKey="Mehdi R" first="R" last="Mehdi">R. Mehdi</name></author><author><name sortKey="Abdullah, B" sort="Abdullah, B" uniqKey="Abdullah B" first="B" last="Abdullah">B. Abdullah</name></author><author><name sortKey="Zurainee, M N" sort="Zurainee, M N" uniqKey="Zurainee M" first="M N" last="Zurainee">M N Zurainee</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1998">1998</date><idno type="RBID">pubmed:9784388</idno><idno type="pmid">9784388</idno><idno type="doi">10.1006/bbrc.1998.9360</idno><idno type="wicri:Area/PubMed/Corpus">004E16</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">004E16</idno><idno type="wicri:Area/PubMed/Curation">004E16</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">004E16</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A Brugia malayi antigen specifically recognized by infected individuals.</title><author><name sortKey="Rahmah, N" sort="Rahmah, N" uniqKey="Rahmah N" first="N" last="Rahmah">N. Rahmah</name><affiliation wicri:level="1"><nlm:affiliation>School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia. rahmah@kb.usm.my</nlm:affiliation><country xml:lang="fr">Malaisie</country><wicri:regionArea>School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan</wicri:regionArea></affiliation></author><author><name sortKey="Anuar, A K" sort="Anuar, A K" uniqKey="Anuar A" first="A K" last="Anuar">A K Anuar</name></author><author><name sortKey="A Shikin, A N" sort="A Shikin, A N" uniqKey="A Shikin A" first="A N" last="A'Shikin">A N A'Shikin</name></author><author><name sortKey="Lim, B H" sort="Lim, B H" uniqKey="Lim B" first="B H" last="Lim">B H Lim</name></author><author><name sortKey="Mehdi, R" sort="Mehdi, R" uniqKey="Mehdi R" first="R" last="Mehdi">R. Mehdi</name></author><author><name sortKey="Abdullah, B" sort="Abdullah, B" uniqKey="Abdullah B" first="B" last="Abdullah">B. Abdullah</name></author><author><name sortKey="Zurainee, M N" sort="Zurainee, M N" uniqKey="Zurainee M" first="M N" last="Zurainee">M N Zurainee</name></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="ISSN">0006-291X</idno><imprint><date when="1998" type="published">1998</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Animals</term><term>Antibodies, Helminth (blood)</term><term>Antibodies, Helminth (immunology)</term><term>Antigens, Helminth (blood)</term><term>Antigens, Helminth (immunology)</term><term>Brugia malayi (immunology)</term><term>Elephantiasis, Filarial (blood)</term><term>Elephantiasis, Filarial (immunology)</term><term>Humans</term><term>Immunoassay (methods)</term><term>Immunoglobulin G (blood)</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte</term><term>Animaux</term><term>Anticorps antihelminthe (immunologie)</term><term>Anticorps antihelminthe (sang)</term><term>Antigènes d'helminthe (immunologie)</term><term>Antigènes d'helminthe (sang)</term><term>Brugia malayi (immunologie)</term><term>Dosage immunologique ()</term><term>Filariose lymphatique (immunologie)</term><term>Filariose lymphatique (sang)</term><term>Humains</term><term>Immunoglobuline G (sang)</term><term>Sensibilité et spécificité</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Helminth</term><term>Antigens, Helminth</term><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Helminth</term><term>Antigens, Helminth</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antihelminthe</term><term>Antigènes d'helminthe</term><term>Brugia malayi</term><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Brugia malayi</term><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Immunoassay</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antihelminthe</term><term>Antigènes d'helminthe</term><term>Filariose lymphatique</term><term>Immunoglobuline G</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Animals</term><term>Humans</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Animaux</term><term>Dosage immunologique</term><term>Humains</term><term>Sensibilité et spécificité</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Western blot analyses were performed on 444 serum specimens: 40 sera from microfilaraemic individuals, 10 sera from elephantiasis patients, 24 treated individuals, 50 sera from residents of endemic areas without anti-filarial IgG4 antibodies (endemic normals), 20 sera from amicrofilaraemic individuals with high anti-filarial IgG4 antibodies, 200 sera from healthy city-dwellers (non-endemic samples), and 100 sera from soil-transmitted helminth-infected individuals. Phast electrophoresis system was used to electrophorese Brugia malayi soluble adult worm antigen on 10-15% SDS-PAGE gradient gels followed by electrophoretic transfer onto PVDF membranes. Membrane strips were then successively incubated with blocking solution, human sera, and monoclonal anti-human IgG4 antibody-HRP, with adequate washings done in between each incubation step. Luminol chemiluminescence detection was then used to develop the blots. An antigenic band with the MW of approximately 37 kDa was found to be consistently present in the Western blots of all microfilaraemic sera, all amicrofilaraemic sera with high titres of anti-filarial IgG4 antibodies, some treated patients, and some elephantiasis patients. The antigen did not occur in immunoblots of individuals with other helminthic infections, normal endemic individuals, and city dwellers. Therefore the B. malayi antigen of with the MW of approximately 37 kDa demonstrated specific reactions with sera of B. malayi-infected individuals and thus may be useful for diagnostic application.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">9784388</PMID><DateCreated><Year>1998</Year><Month>11</Month><Day>23</Day></DateCreated><DateCompleted><Year>1998</Year><Month>11</Month><Day>23</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0006-291X</ISSN><JournalIssue CitedMedium="Print"><Volume>250</Volume><Issue>3</Issue><PubDate><Year>1998</Year><Month>Sep</Month><Day>29</Day></PubDate></JournalIssue><Title>Biochemical and biophysical research communications</Title><ISOAbbreviation>Biochem. Biophys. Res. Commun.</ISOAbbreviation></Journal><ArticleTitle>A Brugia malayi antigen specifically recognized by infected individuals.</ArticleTitle><Pagination><MedlinePgn>586-8</MedlinePgn></Pagination><Abstract><AbstractText>Western blot analyses were performed on 444 serum specimens: 40 sera from microfilaraemic individuals, 10 sera from elephantiasis patients, 24 treated individuals, 50 sera from residents of endemic areas without anti-filarial IgG4 antibodies (endemic normals), 20 sera from amicrofilaraemic individuals with high anti-filarial IgG4 antibodies, 200 sera from healthy city-dwellers (non-endemic samples), and 100 sera from soil-transmitted helminth-infected individuals. Phast electrophoresis system was used to electrophorese Brugia malayi soluble adult worm antigen on 10-15% SDS-PAGE gradient gels followed by electrophoretic transfer onto PVDF membranes. Membrane strips were then successively incubated with blocking solution, human sera, and monoclonal anti-human IgG4 antibody-HRP, with adequate washings done in between each incubation step. Luminol chemiluminescence detection was then used to develop the blots. An antigenic band with the MW of approximately 37 kDa was found to be consistently present in the Western blots of all microfilaraemic sera, all amicrofilaraemic sera with high titres of anti-filarial IgG4 antibodies, some treated patients, and some elephantiasis patients. The antigen did not occur in immunoblots of individuals with other helminthic infections, normal endemic individuals, and city dwellers. Therefore the B. malayi antigen of with the MW of approximately 37 kDa demonstrated specific reactions with sera of B. malayi-infected individuals and thus may be useful for diagnostic application.</AbstractText><CopyrightInformation>Copyright 1998 Academic Press.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rahmah</LastName><ForeName>N</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia. rahmah@kb.usm.my</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Anuar</LastName><ForeName>A K</ForeName><Initials>AK</Initials></Author><Author ValidYN="Y"><LastName>A'shikin</LastName><ForeName>A N</ForeName><Initials>AN</Initials></Author><Author ValidYN="Y"><LastName>Lim</LastName><ForeName>B H</ForeName><Initials>BH</Initials></Author><Author ValidYN="Y"><LastName>Mehdi</LastName><ForeName>R</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Abdullah</LastName><ForeName>B</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Zurainee</LastName><ForeName>M N</ForeName><Initials>MN</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biochem Biophys Res Commun</MedlineTA><NlmUniqueID>0372516</NlmUniqueID><ISSNLinking>0006-291X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000909">Antibodies, Helminth</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000947">Antigens, Helminth</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007074">Immunoglobulin G</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000909" MajorTopicYN="N">Antibodies, Helminth</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000947" MajorTopicYN="N">Antigens, Helminth</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017178" MajorTopicYN="N">Brugia malayi</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004605" MajorTopicYN="N">Elephantiasis, Filarial</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007118" MajorTopicYN="N">Immunoassay</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007074" MajorTopicYN="N">Immunoglobulin G</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1998</Year><Month>10</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1998</Year><Month>10</Month><Day>24</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1998</Year><Month>10</Month><Day>24</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">9784388</ArticleId><ArticleId IdType="pii">S0006-291X(98)99360-3</ArticleId><ArticleId IdType="doi">10.1006/bbrc.1998.9360</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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