Detection of filaria-specific IgG4 antibodies and filarial DNA, for the screening of blood spots for Brugia timori.
Identifieur interne : 003D70 ( PubMed/Curation ); précédent : 003D69; suivant : 003D71Detection of filaria-specific IgG4 antibodies and filarial DNA, for the screening of blood spots for Brugia timori.
Auteurs : P. Fischer [Allemagne] ; I. Bonow ; T. Supali ; P. Rückert ; N. RahmahSource :
- Annals of tropical medicine and parasitology [ 0003-4983 ] ; 2005.
Descripteurs français
- KwdFr :
- ADN des helminthes (analyse), Animaux, Anticorps antihelminthe (sang), Brugia (immunologie), Brugia (isolement et purification), Brugia malayi (immunologie), Filariose lymphatique (immunologie), Filariose lymphatique (parasitologie), Humains, Immunoglobuline G (sang), Microfilaria (immunologie), Microfilaria (isolement et purification), Réaction de polymérisation en chaîne (), Spécificité des anticorps (immunologie), Tests diagnostiques courants ().
- MESH :
- analyse : ADN des helminthes.
- immunologie : Brugia, Brugia malayi, Filariose lymphatique, Microfilaria, Spécificité des anticorps.
- isolement et purification : Brugia, Microfilaria.
- parasitologie : Filariose lymphatique.
- sang : Anticorps antihelminthe, Immunoglobuline G.
- Animaux, Humains, Réaction de polymérisation en chaîne, Tests diagnostiques courants.
English descriptors
- KwdEn :
- Animals, Antibodies, Helminth (blood), Antibody Specificity (immunology), Brugia (immunology), Brugia (isolation & purification), Brugia malayi (immunology), DNA, Helminth (analysis), Diagnostic Tests, Routine (methods), Elephantiasis, Filarial (immunology), Elephantiasis, Filarial (parasitology), Humans, Immunoglobulin G (blood), Microfilariae (immunology), Microfilariae (isolation & purification), Polymerase Chain Reaction (methods).
- MESH :
- chemical , analysis : DNA, Helminth.
- chemical , blood : Antibodies, Helminth, Immunoglobulin G.
- immunology : Antibody Specificity, Brugia, Brugia malayi, Elephantiasis, Filarial, Microfilariae.
- isolation & purification : Brugia, Microfilariae.
- methods : Diagnostic Tests, Routine, Polymerase Chain Reaction.
- parasitology : Elephantiasis, Filarial.
- Animals, Humans.
Abstract
The establishment of simple, sensitive and specific tools for the diagnosis of brugian lymphatic filariasis is a prerequisite for a successful intervention to control the disease. In the simple and rapid Brugia Rapid (BR) test, an immunochromatographic dipstick is used to detect IgG(4) antibodies that are reactive with a recombinant Brugia malayi antigen. When sera from 109 individuals with Brugia microfilaraemias (12 with B. malayi and 97 with B. timori) were investigated using the BR test, all were found positive. In contrast, all of the 150 sera from individuals with Onchocerca volvulus or Mansonella infections investigated were found negative in BR tests. Some unwelcome cross-reactions were observed, however, with sera from individuals infected with Wuchereria bancrofti (three of 12 test-positive) and Dirofilaria (one of nine test-positive). In an attempt to facilitate sample collection and detect any cross-reactions, the BR dipstick was used to screen blood spots, that had been allowed to dry on filter paper, for B. timori microfilariae, before the dipstick-positive samples were tested with a PCR-based assay. Of the 66 individuals so tested, 37 (56%) were found positive by the BR test used on dry blood spots and eight (22%) by the filtration of fresh blood samples. Only nine of the 37 dipstick-positive samples were found PCR-positive. The combined use of BR tests and PCR-based assays, for testing blood spots in areas where brugian filariasis is endemic, appears to be a promising method not only for post-treatment monitoring but also for the certification activities planned within the framework of the Global Programme to Eliminate Lymphatic Filariasis.
DOI: 10.1179/136485905X13339
PubMed: 15701256
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pubmed:15701256Le document en format XML
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<term>DNA, Helminth (analysis)</term>
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<front><div type="abstract" xml:lang="en">The establishment of simple, sensitive and specific tools for the diagnosis of brugian lymphatic filariasis is a prerequisite for a successful intervention to control the disease. In the simple and rapid Brugia Rapid (BR) test, an immunochromatographic dipstick is used to detect IgG(4) antibodies that are reactive with a recombinant Brugia malayi antigen. When sera from 109 individuals with Brugia microfilaraemias (12 with B. malayi and 97 with B. timori) were investigated using the BR test, all were found positive. In contrast, all of the 150 sera from individuals with Onchocerca volvulus or Mansonella infections investigated were found negative in BR tests. Some unwelcome cross-reactions were observed, however, with sera from individuals infected with Wuchereria bancrofti (three of 12 test-positive) and Dirofilaria (one of nine test-positive). In an attempt to facilitate sample collection and detect any cross-reactions, the BR dipstick was used to screen blood spots, that had been allowed to dry on filter paper, for B. timori microfilariae, before the dipstick-positive samples were tested with a PCR-based assay. Of the 66 individuals so tested, 37 (56%) were found positive by the BR test used on dry blood spots and eight (22%) by the filtration of fresh blood samples. Only nine of the 37 dipstick-positive samples were found PCR-positive. The combined use of BR tests and PCR-based assays, for testing blood spots in areas where brugian filariasis is endemic, appears to be a promising method not only for post-treatment monitoring but also for the certification activities planned within the framework of the Global Programme to Eliminate Lymphatic Filariasis.</div>
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<Abstract><AbstractText>The establishment of simple, sensitive and specific tools for the diagnosis of brugian lymphatic filariasis is a prerequisite for a successful intervention to control the disease. In the simple and rapid Brugia Rapid (BR) test, an immunochromatographic dipstick is used to detect IgG(4) antibodies that are reactive with a recombinant Brugia malayi antigen. When sera from 109 individuals with Brugia microfilaraemias (12 with B. malayi and 97 with B. timori) were investigated using the BR test, all were found positive. In contrast, all of the 150 sera from individuals with Onchocerca volvulus or Mansonella infections investigated were found negative in BR tests. Some unwelcome cross-reactions were observed, however, with sera from individuals infected with Wuchereria bancrofti (three of 12 test-positive) and Dirofilaria (one of nine test-positive). In an attempt to facilitate sample collection and detect any cross-reactions, the BR dipstick was used to screen blood spots, that had been allowed to dry on filter paper, for B. timori microfilariae, before the dipstick-positive samples were tested with a PCR-based assay. Of the 66 individuals so tested, 37 (56%) were found positive by the BR test used on dry blood spots and eight (22%) by the filtration of fresh blood samples. Only nine of the 37 dipstick-positive samples were found PCR-positive. The combined use of BR tests and PCR-based assays, for testing blood spots in areas where brugian filariasis is endemic, appears to be a promising method not only for post-treatment monitoring but also for the certification activities planned within the framework of the Global Programme to Eliminate Lymphatic Filariasis.</AbstractText>
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