Evaluation of synthetic peptides of WbSXP-1 for the diagnosis of human lymphatic filariasis.
Identifieur interne : 002840 ( PubMed/Curation ); précédent : 002839; suivant : 002841Evaluation of synthetic peptides of WbSXP-1 for the diagnosis of human lymphatic filariasis.
Auteurs : Pandurangan Pandiaraja [Inde] ; Chakkaravarthy Arunkumar ; Subhash Laxmanappa Hoti ; Donthamsetty Nageswara Rao ; Perumal KalirajSource :
- Diagnostic microbiology and infectious disease [ 1879-0070 ] ; 2010.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antihelminthe (sang), Antigènes d'helminthe (), Antigènes d'helminthe (génétique), Antigènes d'helminthe (immunologie), Antigènes d'helminthe (métabolisme), Données de séquences moléculaires, Filariose lymphatique (diagnostic), Filariose lymphatique (immunologie), Filariose lymphatique (parasitologie), Humains, Peptides (), Peptides (immunologie), Peptides (synthèse chimique), Protéines recombinantes (génétique), Protéines recombinantes (immunologie), Protéines recombinantes (métabolisme), Séquence d'acides aminés, Test ELISA (), Wuchereria bancrofti (immunologie), Épitopes immunodominants (), Épitopes immunodominants (génétique), Épitopes immunodominants (immunologie).
- MESH :
- diagnostic : Filariose lymphatique.
- génétique : Antigènes d'helminthe, Protéines recombinantes, Épitopes immunodominants.
- immunologie : Antigènes d'helminthe, Filariose lymphatique, Peptides, Protéines recombinantes, Wuchereria bancrofti, Épitopes immunodominants.
- métabolisme : Antigènes d'helminthe, Protéines recombinantes.
- parasitologie : Filariose lymphatique.
- sang : Anticorps antihelminthe.
- synthèse chimique : Peptides.
- Animaux, Antigènes d'helminthe, Données de séquences moléculaires, Humains, Peptides, Séquence d'acides aminés, Test ELISA, Épitopes immunodominants.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Helminth (blood), Antigens, Helminth (chemistry), Antigens, Helminth (genetics), Antigens, Helminth (immunology), Antigens, Helminth (metabolism), Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (immunology), Elephantiasis, Filarial (parasitology), Enzyme-Linked Immunosorbent Assay (methods), Humans, Immunodominant Epitopes (chemistry), Immunodominant Epitopes (genetics), Immunodominant Epitopes (immunology), Molecular Sequence Data, Peptides (chemical synthesis), Peptides (chemistry), Peptides (immunology), Recombinant Proteins (genetics), Recombinant Proteins (immunology), Recombinant Proteins (metabolism), Wuchereria bancrofti (immunology).
- MESH :
- chemical , blood : Antibodies, Helminth.
- chemical , chemical synthesis : Peptides.
- chemical , chemistry : Antigens, Helminth, Immunodominant Epitopes, Peptides.
- chemical , genetics : Antigens, Helminth, Immunodominant Epitopes, Recombinant Proteins.
- chemical , immunology : Antigens, Helminth, Immunodominant Epitopes, Peptides, Recombinant Proteins.
- chemical , metabolism : Antigens, Helminth, Recombinant Proteins.
- diagnosis : Elephantiasis, Filarial.
- immunology : Elephantiasis, Filarial, Wuchereria bancrofti.
- methods : Enzyme-Linked Immunosorbent Assay.
- parasitology : Elephantiasis, Filarial.
- Amino Acid Sequence, Animals, Humans, Molecular Sequence Data.
Abstract
Parasitic nematodes infect nearly half of the world's human population, resulting in significant morbidity and mortality. Though filariasis is not fatal, it is the second leading cause of permanent and long-term disability worldwide. Filariasis has a spectrum of disease manifestation and infectivity found among the infected individuals and also goes unnoticed for years. Furthermore, there are ample reports emerging on the genetic variation among the parasites population. Hence, it is necessary to develop diagnostics for early detection of the disease. Synthetic peptides that mimic the immunogenic regions and a conserved region similar to that of recombinant antigen will be more useful in developing diagnostics, vaccines, or therapeutics. WbSXP-1 was earlier proven as a good diagnostic antigen; B-cell epitopic analysis showed 4 potent immunodominant regions spanning the whole antigen. These synthetic peptides (N, N1, N2, and N3) were produced and used as a diagnostic candidate to detect anti-SXP antibody and conversely to detect the infected individuals. The monomeric peptides showed good reactivity against microfilareamic (MF) sera. Among them, the peptides N, N1, and N2 were found to be more reactive. Furthermore, multiple chimeric peptides in linear combinations of 2 peptides were tested for its efficacy to detect anti-SXP antibody in infected MF sera. The peptides N:N1 and N1:N2 were synthesized and tested against human clinical sera. This chimeric peptides constructed based on WbSXP-1 were found to be reactive, specifically with MF sera by ELISA. These peptide-based diagnostic method can serve as a standard better tool without cross-reactivity in lymphatic filariasis elimination program.
DOI: 10.1016/j.diagmicrobio.2010.07.015
PubMed: 21094425
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Though filariasis is not fatal, it is the second leading cause of permanent and long-term disability worldwide. Filariasis has a spectrum of disease manifestation and infectivity found among the infected individuals and also goes unnoticed for years. Furthermore, there are ample reports emerging on the genetic variation among the parasites population. Hence, it is necessary to develop diagnostics for early detection of the disease. Synthetic peptides that mimic the immunogenic regions and a conserved region similar to that of recombinant antigen will be more useful in developing diagnostics, vaccines, or therapeutics. WbSXP-1 was earlier proven as a good diagnostic antigen; B-cell epitopic analysis showed 4 potent immunodominant regions spanning the whole antigen. These synthetic peptides (N, N1, N2, and N3) were produced and used as a diagnostic candidate to detect anti-SXP antibody and conversely to detect the infected individuals. The monomeric peptides showed good reactivity against microfilareamic (MF) sera. Among them, the peptides N, N1, and N2 were found to be more reactive. Furthermore, multiple chimeric peptides in linear combinations of 2 peptides were tested for its efficacy to detect anti-SXP antibody in infected MF sera. The peptides N:N1 and N1:N2 were synthesized and tested against human clinical sera. This chimeric peptides constructed based on WbSXP-1 were found to be reactive, specifically with MF sera by ELISA. These peptide-based diagnostic method can serve as a standard better tool without cross-reactivity in lymphatic filariasis elimination program.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21094425</PMID><DateCreated><Year>2010</Year><Month>11</Month><Day>24</Day></DateCreated><DateCompleted><Year>2011</Year><Month>03</Month><Day>21</Day></DateCompleted><DateRevised><Year>2010</Year><Month>11</Month><Day>24</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1879-0070</ISSN><JournalIssue CitedMedium="Internet"><Volume>68</Volume><Issue>4</Issue><PubDate><Year>2010</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Diagnostic microbiology and infectious disease</Title><ISOAbbreviation>Diagn. Microbiol. Infect. 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Synthetic peptides that mimic the immunogenic regions and a conserved region similar to that of recombinant antigen will be more useful in developing diagnostics, vaccines, or therapeutics. WbSXP-1 was earlier proven as a good diagnostic antigen; B-cell epitopic analysis showed 4 potent immunodominant regions spanning the whole antigen. These synthetic peptides (N, N1, N2, and N3) were produced and used as a diagnostic candidate to detect anti-SXP antibody and conversely to detect the infected individuals. The monomeric peptides showed good reactivity against microfilareamic (MF) sera. Among them, the peptides N, N1, and N2 were found to be more reactive. Furthermore, multiple chimeric peptides in linear combinations of 2 peptides were tested for its efficacy to detect anti-SXP antibody in infected MF sera. The peptides N:N1 and N1:N2 were synthesized and tested against human clinical sera. This chimeric peptides constructed based on WbSXP-1 were found to be reactive, specifically with MF sera by ELISA. These peptide-based diagnostic method can serve as a standard better tool without cross-reactivity in lymphatic filariasis elimination program.</AbstractText><CopyrightInformation>Copyright © 2010 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pandiaraja</LastName><ForeName>Pandurangan</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Centre for Biotechnology, Anna University, Chennai, Tamil Nadu, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Arunkumar</LastName><ForeName>Chakkaravarthy</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Hoti</LastName><ForeName>Subhash Laxmanappa</ForeName><Initials>SL</Initials></Author><Author ValidYN="Y"><LastName>Rao</LastName><ForeName>Donthamsetty Nageswara</ForeName><Initials>DN</Initials></Author><Author ValidYN="Y"><LastName>Kaliraj</LastName><ForeName>Perumal</ForeName><Initials>P</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Studies</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType 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