Detection of Wolbachia endobacteria in Culex quinquefasciatus by Gimenez staining and confirmation by PCR.
Identifieur interne : 001C92 ( PubMed/Curation ); précédent : 001C91; suivant : 001C93Detection of Wolbachia endobacteria in Culex quinquefasciatus by Gimenez staining and confirmation by PCR.
Auteurs : M. Muniaraj [Inde] ; R. Paramasivan ; I P Sunish ; N. Arunachalam ; T. Mariappan ; S Victor Jerald Leo ; K J DhananjeyanSource :
- Journal of vector borne diseases [ 0972-9062 ] ; 2012.
Descripteurs français
- KwdFr :
- ADN bactérien (analyse), ADN bactérien (génétique), Animaux, Coloration et marquage (), Culex (), Culex (microbiologie), Femelle, Filariose lymphatique (transmission), Ovaire (microbiologie), Réaction de polymérisation en chaîne (), Vecteurs insectes (microbiologie), Wolbachia (génétique), Wolbachia (isolement et purification).
- MESH :
- analyse : ADN bactérien.
- génétique : ADN bactérien, Wolbachia.
- isolement et purification : Wolbachia.
- microbiologie : Culex, Ovaire, Vecteurs insectes.
- Animaux, Coloration et marquage, Culex, Femelle, Filariose lymphatique, Réaction de polymérisation en chaîne.
English descriptors
- KwdEn :
- Animals, Culex (classification), Culex (microbiology), DNA, Bacterial (analysis), DNA, Bacterial (genetics), Elephantiasis, Filarial (transmission), Female, Insect Vectors (microbiology), Ovary (microbiology), Polymerase Chain Reaction (methods), Staining and Labeling (methods), Wolbachia (genetics), Wolbachia (isolation & purification).
- MESH :
- chemical , analysis : DNA, Bacterial.
- classification : Culex.
- chemical , genetics : DNA, Bacterial, Wolbachia.
- isolation & purification : Wolbachia.
- methods : Polymerase Chain Reaction, Staining and Labeling.
- microbiology : Culex, Insect Vectors, Ovary.
- transmission : Elephantiasis, Filarial.
- Animals, Female.
Abstract
Wolbachia are common intracellular bacteria that are found in arthropods and nematodes. These endosymbionts are transmitted vertically through host eggs and alter host biology in diverse ways, including the induction of reproductive manipulations, such as feminization, parthenogenesis, male killing and sperm-egg incompatibility. Since they can also move horizontally across species boundaries, Wolbachia is gaining importance in recent days as it could be used as a biological control agent to control vector mosquitoes or for paratransgenic approaches. However, the study of Wolbachia requires sophisticated techniques such as PCR and cell culture facilities which cannot be affordable for many laboratories where the diseases transmitted by arthropod vectors are common. Hence, it would be beneficial to develop a simple method to detect the presence of Wolbachia in arthropods.
PubMed: 23428527
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001C92
Links to Exploration step
pubmed:23428527Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Detection of Wolbachia endobacteria in Culex quinquefasciatus by Gimenez staining and confirmation by PCR.</title><author><name sortKey="Muniaraj, M" sort="Muniaraj, M" uniqKey="Muniaraj M" first="M" last="Muniaraj">M. Muniaraj</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Research in Medical Entomology (ICMR), Madurai, Tamil Nadu, India. mmuniaraj@gmail.com</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Research in Medical Entomology (ICMR), Madurai, Tamil Nadu</wicri:regionArea></affiliation></author><author><name sortKey="Paramasivan, R" sort="Paramasivan, R" uniqKey="Paramasivan R" first="R" last="Paramasivan">R. Paramasivan</name></author><author><name sortKey="Sunish, I P" sort="Sunish, I P" uniqKey="Sunish I" first="I P" last="Sunish">I P Sunish</name></author><author><name sortKey="Arunachalam, N" sort="Arunachalam, N" uniqKey="Arunachalam N" first="N" last="Arunachalam">N. Arunachalam</name></author><author><name sortKey="Mariappan, T" sort="Mariappan, T" uniqKey="Mariappan T" first="T" last="Mariappan">T. Mariappan</name></author><author><name sortKey="Jerald Leo, S Victor" sort="Jerald Leo, S Victor" uniqKey="Jerald Leo S" first="S Victor" last="Jerald Leo">S Victor Jerald Leo</name></author><author><name sortKey="Dhananjeyan, K J" sort="Dhananjeyan, K J" uniqKey="Dhananjeyan K" first="K J" last="Dhananjeyan">K J Dhananjeyan</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:23428527</idno><idno type="pmid">23428527</idno><idno type="wicri:Area/PubMed/Corpus">001C92</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001C92</idno><idno type="wicri:Area/PubMed/Curation">001C92</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001C92</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Detection of Wolbachia endobacteria in Culex quinquefasciatus by Gimenez staining and confirmation by PCR.</title><author><name sortKey="Muniaraj, M" sort="Muniaraj, M" uniqKey="Muniaraj M" first="M" last="Muniaraj">M. Muniaraj</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Research in Medical Entomology (ICMR), Madurai, Tamil Nadu, India. mmuniaraj@gmail.com</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Research in Medical Entomology (ICMR), Madurai, Tamil Nadu</wicri:regionArea></affiliation></author><author><name sortKey="Paramasivan, R" sort="Paramasivan, R" uniqKey="Paramasivan R" first="R" last="Paramasivan">R. Paramasivan</name></author><author><name sortKey="Sunish, I P" sort="Sunish, I P" uniqKey="Sunish I" first="I P" last="Sunish">I P Sunish</name></author><author><name sortKey="Arunachalam, N" sort="Arunachalam, N" uniqKey="Arunachalam N" first="N" last="Arunachalam">N. Arunachalam</name></author><author><name sortKey="Mariappan, T" sort="Mariappan, T" uniqKey="Mariappan T" first="T" last="Mariappan">T. Mariappan</name></author><author><name sortKey="Jerald Leo, S Victor" sort="Jerald Leo, S Victor" uniqKey="Jerald Leo S" first="S Victor" last="Jerald Leo">S Victor Jerald Leo</name></author><author><name sortKey="Dhananjeyan, K J" sort="Dhananjeyan, K J" uniqKey="Dhananjeyan K" first="K J" last="Dhananjeyan">K J Dhananjeyan</name></author></analytic><series><title level="j">Journal of vector borne diseases</title><idno type="ISSN">0972-9062</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Culex (classification)</term><term>Culex (microbiology)</term><term>DNA, Bacterial (analysis)</term><term>DNA, Bacterial (genetics)</term><term>Elephantiasis, Filarial (transmission)</term><term>Female</term><term>Insect Vectors (microbiology)</term><term>Ovary (microbiology)</term><term>Polymerase Chain Reaction (methods)</term><term>Staining and Labeling (methods)</term><term>Wolbachia (genetics)</term><term>Wolbachia (isolation & purification)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (analyse)</term><term>ADN bactérien (génétique)</term><term>Animaux</term><term>Coloration et marquage ()</term><term>Culex ()</term><term>Culex (microbiologie)</term><term>Femelle</term><term>Filariose lymphatique (transmission)</term><term>Ovaire (microbiologie)</term><term>Réaction de polymérisation en chaîne ()</term><term>Vecteurs insectes (microbiologie)</term><term>Wolbachia (génétique)</term><term>Wolbachia (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Bacterial</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN bactérien</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Culex</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Bacterial</term><term>Wolbachia</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN bactérien</term><term>Wolbachia</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Wolbachia</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Wolbachia</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term><term>Staining and Labeling</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Culex</term><term>Ovaire</term><term>Vecteurs insectes</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Culex</term><term>Insect Vectors</term><term>Ovary</term></keywords><keywords scheme="MESH" qualifier="transmission" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Coloration et marquage</term><term>Culex</term><term>Femelle</term><term>Filariose lymphatique</term><term>Réaction de polymérisation en chaîne</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Wolbachia are common intracellular bacteria that are found in arthropods and nematodes. These endosymbionts are transmitted vertically through host eggs and alter host biology in diverse ways, including the induction of reproductive manipulations, such as feminization, parthenogenesis, male killing and sperm-egg incompatibility. Since they can also move horizontally across species boundaries, Wolbachia is gaining importance in recent days as it could be used as a biological control agent to control vector mosquitoes or for paratransgenic approaches. However, the study of Wolbachia requires sophisticated techniques such as PCR and cell culture facilities which cannot be affordable for many laboratories where the diseases transmitted by arthropod vectors are common. Hence, it would be beneficial to develop a simple method to detect the presence of Wolbachia in arthropods.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23428527</PMID><DateCreated><Year>2013</Year><Month>02</Month><Day>22</Day></DateCreated><DateCompleted><Year>2013</Year><Month>11</Month><Day>29</Day></DateCompleted><DateRevised><Year>2013</Year><Month>02</Month><Day>22</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0972-9062</ISSN><JournalIssue CitedMedium="Internet"><Volume>49</Volume><Issue>4</Issue><PubDate><Year>2012</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Journal of vector borne diseases</Title><ISOAbbreviation>J Vector Borne Dis</ISOAbbreviation></Journal><ArticleTitle>Detection of Wolbachia endobacteria in Culex quinquefasciatus by Gimenez staining and confirmation by PCR.</ArticleTitle><Pagination><MedlinePgn>258-61</MedlinePgn></Pagination><Abstract><AbstractText Label="BACKGROUND & OBJECTIVES" NlmCategory="OBJECTIVE">Wolbachia are common intracellular bacteria that are found in arthropods and nematodes. These endosymbionts are transmitted vertically through host eggs and alter host biology in diverse ways, including the induction of reproductive manipulations, such as feminization, parthenogenesis, male killing and sperm-egg incompatibility. Since they can also move horizontally across species boundaries, Wolbachia is gaining importance in recent days as it could be used as a biological control agent to control vector mosquitoes or for paratransgenic approaches. However, the study of Wolbachia requires sophisticated techniques such as PCR and cell culture facilities which cannot be affordable for many laboratories where the diseases transmitted by arthropod vectors are common. Hence, it would be beneficial to develop a simple method to detect the presence of Wolbachia in arthropods.</AbstractText><AbstractText Label="METHOD" NlmCategory="METHODS">In this study, we described a method of staining Wolbachia endobacteria, present in the reproductive tissues of mosquitoes. The reliability of this method was compared with Gram staining and PCR based detection.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The microscopic observation of the Gimenez stained smear prepared from the teased ovary of wild caught and Wolbachia (+) Cx. quinquefasciatus revealed the presence of pink coloured pleomorphic cells of Wolbachia ranging from cocci, comma shaped cells to bacillus and chain forms. The ovaries of Wolbachia (-) cured mosquito did not show any cell. Although Gram's staining is a reliable differential staining for the other bacteria, the bacterial cells in the smears from the ovaries of wild caught mosquitoes did not take the stain properly and the cells were not clearly visible. The PCR amplified product from the pooled remains of wild caught and Wolbachia (+) Cx. quinquefasciatus showed clear banding, whereas, no banding was observed for the negative control (distilled water) and Wolbachia (-) Cx. quinquefasciatus.</AbstractText><AbstractText Label="INTERPRETATION & CONCLUSION" NlmCategory="CONCLUSIONS">The Gimenez staining technique applied, could be used to detect the members of the endobacteria Wolbachia easily, even in a simple laboratory without any special facilities or even in the field condition and for handling large number of samples in a shorter duration.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Muniaraj</LastName><ForeName>M</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Centre for Research in Medical Entomology (ICMR), Madurai, Tamil Nadu, India. mmuniaraj@gmail.com</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Paramasivan</LastName><ForeName>R</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Sunish</LastName><ForeName>I P</ForeName><Initials>IP</Initials></Author><Author ValidYN="Y"><LastName>Arunachalam</LastName><ForeName>N</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Mariappan</LastName><ForeName>T</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Jerald Leo</LastName><ForeName>S Victor</ForeName><Initials>SV</Initials></Author><Author ValidYN="Y"><LastName>Dhananjeyan</LastName><ForeName>K J</ForeName><Initials>KJ</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>India</Country><MedlineTA>J Vector Borne Dis</MedlineTA><NlmUniqueID>101212761</NlmUniqueID><ISSNLinking>0972-9062</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004269">DNA, Bacterial</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003465" MajorTopicYN="N">Culex</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004269" MajorTopicYN="N">DNA, Bacterial</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004605" MajorTopicYN="N">Elephantiasis, Filarial</DescriptorName><QualifierName UI="Q000635" MajorTopicYN="N">transmission</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007303" MajorTopicYN="N">Insect Vectors</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010053" MajorTopicYN="N">Ovary</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013194" MajorTopicYN="N">Staining and Labeling</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020577" MajorTopicYN="N">Wolbachia</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>2</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>2</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>12</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23428527</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/PubMed/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001C92 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd -nk 001C92 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= PubMed
|étape= Curation
|type= RBID
|clé= pubmed:23428527
|texte= Detection of Wolbachia endobacteria in Culex quinquefasciatus by Gimenez staining and confirmation by PCR.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Curation/RBID.i -Sk "pubmed:23428527" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |