IgE responses in human filariasis. III. Specificities of IgE and IgG antibodies compared by immunoblot analysis.
Identifieur interne : 006761 ( PubMed/Corpus ); précédent : 006760; suivant : 006762IgE responses in human filariasis. III. Specificities of IgE and IgG antibodies compared by immunoblot analysis.
Auteurs : R. Hussain ; E A OttesenSource :
- Journal of immunology (Baltimore, Md. : 1950) [ 0022-1767 ] ; 1985.
English descriptors
- KwdEn :
- Adolescent, Adult, Antibody Specificity, Antigens, Helminth (immunology), Brugia (immunology), Chronic Disease, Collodion, Electrophoresis, Polyacrylamide Gel, Elephantiasis, Filarial (immunology), Female, Filariasis (immunology), Filariasis (pathology), Humans, Immunoglobulin E (biosynthesis), Immunoglobulin G (biosynthesis), Male, Middle Aged, Paper, Pulmonary Eosinophilia (immunology), Sodium Dodecyl Sulfate, Wuchereria bancrofti (immunology).
- MESH :
- chemical , biosynthesis : Immunoglobulin E, Immunoglobulin G.
- chemical , immunology : Antigens, Helminth.
- immunology : Brugia, Elephantiasis, Filarial, Filariasis, Pulmonary Eosinophilia, Wuchereria bancrofti.
- pathology : Filariasis.
- Adolescent, Adult, Antibody Specificity, Chronic Disease, Collodion, Electrophoresis, Polyacrylamide Gel, Female, Humans, Male, Middle Aged, Paper, Sodium Dodecyl Sulfate.
Abstract
IgE and IgG immune responses were qualitatively characterized in three well-defined groups of patients with different clinical manifestations of lymphatic filariasis caused by infection with Wuchereria bancrofti; viz. tropical pulmonary eosinophilia (TPE), chronic lymphatic obstruction with elephantiasis (CP), and asymptomatic microfilaremia (MF). A complex filarial antigen preparation extracted from adult filarial parasites was separated on SDS-polyacrylamide gels, and was electrophoretically transferred to nitrocellulose paper before being incubated with individual sera and being probed with radiolabeled anti-IgE or protein A. Three clinical groups of patients showed distinct patterns of antigen recognition by both IgE and IgG antibodies, with TPE patients showing the most diverse patterns and microfilaremic individuals the most restricted responses for both antibody isotypes. "Dual recognition" of antigens by IgE and IgG antibodies seemed to be the rule rather than the exception for each individual's immune response to the parasite antigens, but the relative magnitudes of IgG and IgE responses differed among the three groups. The ratio of IgG to IgE antibody was generally greater in patients with MF and CP than in those with TPE. These findings of the comparative specificities and relative abundance of IgE and IgG antibodies in infected patients may have fundamental importance in explaining the clinical expression (especially allergic reactivity) and disease pathogenesis of human filarial infections.
PubMed: 3891853
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pubmed:3891853Le document en format XML
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Specificities of IgE and IgG antibodies compared by immunoblot analysis.</title><author><name sortKey="Hussain, R" sort="Hussain, R" uniqKey="Hussain R" first="R" last="Hussain">R. Hussain</name></author><author><name sortKey="Ottesen, E A" sort="Ottesen, E A" uniqKey="Ottesen E" first="E A" last="Ottesen">E A Ottesen</name></author></analytic><series><title level="j">Journal of immunology (Baltimore, Md. : 1950)</title><idno type="ISSN">0022-1767</idno><imprint><date when="1985" type="published">1985</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Antibody Specificity</term><term>Antigens, Helminth (immunology)</term><term>Brugia (immunology)</term><term>Chronic Disease</term><term>Collodion</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Elephantiasis, Filarial (immunology)</term><term>Female</term><term>Filariasis (immunology)</term><term>Filariasis (pathology)</term><term>Humans</term><term>Immunoglobulin E (biosynthesis)</term><term>Immunoglobulin G (biosynthesis)</term><term>Male</term><term>Middle Aged</term><term>Paper</term><term>Pulmonary Eosinophilia (immunology)</term><term>Sodium Dodecyl Sulfate</term><term>Wuchereria bancrofti (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Immunoglobulin E</term><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Helminth</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Brugia</term><term>Elephantiasis, Filarial</term><term>Filariasis</term><term>Pulmonary Eosinophilia</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Filariasis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Antibody Specificity</term><term>Chronic Disease</term><term>Collodion</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Female</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Paper</term><term>Sodium Dodecyl Sulfate</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">IgE and IgG immune responses were qualitatively characterized in three well-defined groups of patients with different clinical manifestations of lymphatic filariasis caused by infection with Wuchereria bancrofti; viz. tropical pulmonary eosinophilia (TPE), chronic lymphatic obstruction with elephantiasis (CP), and asymptomatic microfilaremia (MF). A complex filarial antigen preparation extracted from adult filarial parasites was separated on SDS-polyacrylamide gels, and was electrophoretically transferred to nitrocellulose paper before being incubated with individual sera and being probed with radiolabeled anti-IgE or protein A. Three clinical groups of patients showed distinct patterns of antigen recognition by both IgE and IgG antibodies, with TPE patients showing the most diverse patterns and microfilaremic individuals the most restricted responses for both antibody isotypes. "Dual recognition" of antigens by IgE and IgG antibodies seemed to be the rule rather than the exception for each individual's immune response to the parasite antigens, but the relative magnitudes of IgG and IgE responses differed among the three groups. The ratio of IgG to IgE antibody was generally greater in patients with MF and CP than in those with TPE. These findings of the comparative specificities and relative abundance of IgE and IgG antibodies in infected patients may have fundamental importance in explaining the clinical expression (especially allergic reactivity) and disease pathogenesis of human filarial infections.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">3891853</PMID><DateCreated><Year>1985</Year><Month>08</Month><Day>19</Day></DateCreated><DateCompleted><Year>1985</Year><Month>08</Month><Day>19</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-1767</ISSN><JournalIssue CitedMedium="Print"><Volume>135</Volume><Issue>2</Issue><PubDate><Year>1985</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Journal of immunology (Baltimore, Md. : 1950)</Title><ISOAbbreviation>J. Immunol.</ISOAbbreviation></Journal><ArticleTitle>IgE responses in human filariasis. III. Specificities of IgE and IgG antibodies compared by immunoblot analysis.</ArticleTitle><Pagination><MedlinePgn>1415-20</MedlinePgn></Pagination><Abstract><AbstractText>IgE and IgG immune responses were qualitatively characterized in three well-defined groups of patients with different clinical manifestations of lymphatic filariasis caused by infection with Wuchereria bancrofti; viz. tropical pulmonary eosinophilia (TPE), chronic lymphatic obstruction with elephantiasis (CP), and asymptomatic microfilaremia (MF). A complex filarial antigen preparation extracted from adult filarial parasites was separated on SDS-polyacrylamide gels, and was electrophoretically transferred to nitrocellulose paper before being incubated with individual sera and being probed with radiolabeled anti-IgE or protein A. Three clinical groups of patients showed distinct patterns of antigen recognition by both IgE and IgG antibodies, with TPE patients showing the most diverse patterns and microfilaremic individuals the most restricted responses for both antibody isotypes. "Dual recognition" of antigens by IgE and IgG antibodies seemed to be the rule rather than the exception for each individual's immune response to the parasite antigens, but the relative magnitudes of IgG and IgE responses differed among the three groups. The ratio of IgG to IgE antibody was generally greater in patients with MF and CP than in those with TPE. These findings of the comparative specificities and relative abundance of IgE and IgG antibodies in infected patients may have fundamental importance in explaining the clinical expression (especially allergic reactivity) and disease pathogenesis of human filarial infections.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hussain</LastName><ForeName>R</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Ottesen</LastName><ForeName>E A</ForeName><Initials>EA</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Immunol</MedlineTA><NlmUniqueID>2985117R</NlmUniqueID><ISSNLinking>0022-1767</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000947">Antigens, Helminth</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007074">Immunoglobulin G</NameOfSubstance></Chemical><Chemical><RegistryNumber>368GB5141J</RegistryNumber><NameOfSubstance UI="D012967">Sodium Dodecyl Sulfate</NameOfSubstance></Chemical><Chemical><RegistryNumber>37341-29-0</RegistryNumber><NameOfSubstance UI="D007073">Immunoglobulin E</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-70-0</RegistryNumber><NameOfSubstance UI="D003101">Collodion</NameOfSubstance></Chemical></ChemicalList><CitationSubset>AIM</CitationSubset><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000293" MajorTopicYN="N">Adolescent</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000918" MajorTopicYN="Y">Antibody Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000947" MajorTopicYN="N">Antigens, Helminth</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002009" MajorTopicYN="N">Brugia</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002908" MajorTopicYN="N">Chronic Disease</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003101" MajorTopicYN="N">Collodion</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004591" MajorTopicYN="N">Electrophoresis, Polyacrylamide Gel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004605" MajorTopicYN="N">Elephantiasis, Filarial</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005368" MajorTopicYN="N">Filariasis</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007073" MajorTopicYN="N">Immunoglobulin E</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007074" MajorTopicYN="N">Immunoglobulin G</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010209" MajorTopicYN="N">Paper</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011657" MajorTopicYN="N">Pulmonary Eosinophilia</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012967" MajorTopicYN="N">Sodium Dodecyl Sulfate</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014958" MajorTopicYN="N">Wuchereria bancrofti</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1985</Year><Month>8</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1985</Year><Month>8</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1985</Year><Month>8</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">3891853</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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