LymphedemaV1, PubMed, Corpus, bibRecord, 006610

A DNA probe cloned in Escherichia coli for the identification of Brugia malayi.

Identifieur interne : 006610 ( PubMed/Corpus ); précédent : 006609; suivant : 006611

A DNA probe cloned in Escherichia coli for the identification of Brugia malayi.

Auteurs : B K Sim ; W F Piessens ; D F Wirth

Source :

Molecular and biochemical parasitology [ 0166-6851 ] ; 1986.

RBID : pubmed:2425257

English descriptors

KwdEn :
- Animals, Brugia (classification), Brugia (genetics), Cloning, Molecular, DNA (analysis), DNA (genetics), Dirofilaria immitis (genetics), Dogs, Elephantiasis, Filarial (parasitology), Escherichia coli (genetics), Gerbillinae, Mice, Mice, Inbred C3H, Mice, Nude, Microfilariae (genetics), Nucleic Acid Hybridization, RNA (genetics), Species Specificity.
MESH :
- chemical , analysis : DNA.
- classification : Brugia.
- genetics : Brugia, DNA, Dirofilaria immitis, Escherichia coli, Microfilariae, RNA.
- parasitology : Elephantiasis, Filarial.
- Animals, Cloning, Molecular, Dogs, Gerbillinae, Mice, Mice, Inbred C3H, Mice, Nude, Nucleic Acid Hybridization, Species Specificity.

Abstract

This report describes a specific and sensitive DNA probe for the identification of Brugia malayi. A genomic DNA library produced from subperiodic B. malayi microfilariae was screened to detect clones containing DNA sequences which are highly repeated within the parasite genome. Several clones were further analyzed to identify those which hybridize specifically with B. malayi DNA but not with DNA from B. pahangi and Dirofilaria immitis. From these, clone pBm15 was selected because it hybridized with high sensitivity to B. malayi DNA as detected by autoradiography. Clone pBm15 was sensitive enough to detect two infective larvae or five microfilariae or 300 pg of purified B. malayi microfilarial DNA. This study forms the basis for the development of a specific and sensitive DNA probe for the identification of B. malayi in field specimens.

PubMed: 2425257

Links to Exploration step

pubmed:2425257

Le document en format XML

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<author><name sortKey="Sim, B K" sort="Sim, B K" uniqKey="Sim B" first="B K" last="Sim">B K Sim</name>
</author>
<author><name sortKey="Piessens, W F" sort="Piessens, W F" uniqKey="Piessens W" first="W F" last="Piessens">W F Piessens</name>
</author>
<author><name sortKey="Wirth, D F" sort="Wirth, D F" uniqKey="Wirth D" first="D F" last="Wirth">D F Wirth</name>
</author>
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<date when="1986">1986</date>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">A DNA probe cloned in Escherichia coli for the identification of Brugia malayi.</title>
<author><name sortKey="Sim, B K" sort="Sim, B K" uniqKey="Sim B" first="B K" last="Sim">B K Sim</name>
</author>
<author><name sortKey="Piessens, W F" sort="Piessens, W F" uniqKey="Piessens W" first="W F" last="Piessens">W F Piessens</name>
</author>
<author><name sortKey="Wirth, D F" sort="Wirth, D F" uniqKey="Wirth D" first="D F" last="Wirth">D F Wirth</name>
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<series><title level="j">Molecular and biochemical parasitology</title>
<idno type="ISSN">0166-6851</idno>
<imprint><date when="1986" type="published">1986</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Brugia (classification)</term>
<term>Brugia (genetics)</term>
<term>Cloning, Molecular</term>
<term>DNA (analysis)</term>
<term>DNA (genetics)</term>
<term>Dirofilaria immitis (genetics)</term>
<term>Dogs</term>
<term>Elephantiasis, Filarial (parasitology)</term>
<term>Escherichia coli (genetics)</term>
<term>Gerbillinae</term>
<term>Mice</term>
<term>Mice, Inbred C3H</term>
<term>Mice, Nude</term>
<term>Microfilariae (genetics)</term>
<term>Nucleic Acid Hybridization</term>
<term>RNA (genetics)</term>
<term>Species Specificity</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA</term>
</keywords>
<keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Brugia</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Brugia</term>
<term>DNA</term>
<term>Dirofilaria immitis</term>
<term>Escherichia coli</term>
<term>Microfilariae</term>
<term>RNA</term>
</keywords>
<keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Elephantiasis, Filarial</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cloning, Molecular</term>
<term>Dogs</term>
<term>Gerbillinae</term>
<term>Mice</term>
<term>Mice, Inbred C3H</term>
<term>Mice, Nude</term>
<term>Nucleic Acid Hybridization</term>
<term>Species Specificity</term>
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<front><div type="abstract" xml:lang="en">This report describes a specific and sensitive DNA probe for the identification of Brugia malayi. A genomic DNA library produced from subperiodic B. malayi microfilariae was screened to detect clones containing DNA sequences which are highly repeated within the parasite genome. Several clones were further analyzed to identify those which hybridize specifically with B. malayi DNA but not with DNA from B. pahangi and Dirofilaria immitis. From these, clone pBm15 was selected because it hybridized with high sensitivity to B. malayi DNA as detected by autoradiography. Clone pBm15 was sensitive enough to detect two infective larvae or five microfilariae or 300 pg of purified B. malayi microfilarial DNA. This study forms the basis for the development of a specific and sensitive DNA probe for the identification of B. malayi in field specimens.</div>
</front>
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<DateCreated><Year>1986</Year>
<Month>08</Month>
<Day>01</Day>
</DateCreated>
<DateCompleted><Year>1986</Year>
<Month>08</Month>
<Day>01</Day>
</DateCompleted>
<DateRevised><Year>2016</Year>
<Month>11</Month>
<Day>23</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0166-6851</ISSN>
<JournalIssue CitedMedium="Print"><Volume>19</Volume>
<Issue>2</Issue>
<PubDate><Year>1986</Year>
<Month>May</Month>
</PubDate>
</JournalIssue>
<Title>Molecular and biochemical parasitology</Title>
<ISOAbbreviation>Mol. Biochem. Parasitol.</ISOAbbreviation>
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<ArticleTitle>A DNA probe cloned in Escherichia coli for the identification of Brugia malayi.</ArticleTitle>
<Pagination><MedlinePgn>117-23</MedlinePgn>
</Pagination>
<Abstract><AbstractText>This report describes a specific and sensitive DNA probe for the identification of Brugia malayi. A genomic DNA library produced from subperiodic B. malayi microfilariae was screened to detect clones containing DNA sequences which are highly repeated within the parasite genome. Several clones were further analyzed to identify those which hybridize specifically with B. malayi DNA but not with DNA from B. pahangi and Dirofilaria immitis. From these, clone pBm15 was selected because it hybridized with high sensitivity to B. malayi DNA as detected by autoradiography. Clone pBm15 was sensitive enough to detect two infective larvae or five microfilariae or 300 pg of purified B. malayi microfilarial DNA. This study forms the basis for the development of a specific and sensitive DNA probe for the identification of B. malayi in field specimens.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Sim</LastName>
<ForeName>B K</ForeName>
<Initials>BK</Initials>
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<Author ValidYN="Y"><LastName>Piessens</LastName>
<ForeName>W F</ForeName>
<Initials>WF</Initials>
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<Author ValidYN="Y"><LastName>Wirth</LastName>
<ForeName>D F</ForeName>
<Initials>DF</Initials>
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<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><GrantID>AI20102</GrantID>
<Acronym>AI</Acronym>
<Agency>NIAID NIH HHS</Agency>
<Country>United States</Country>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
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<MeshHeading><DescriptorName UI="D002009" MajorTopicYN="N">Brugia</DescriptorName>
<QualifierName UI="Q000145" MajorTopicYN="Y">classification</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
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<MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004247" MajorTopicYN="N">DNA</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004183" MajorTopicYN="N">Dirofilaria immitis</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004285" MajorTopicYN="N">Dogs</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004605" MajorTopicYN="N">Elephantiasis, Filarial</DescriptorName>
<QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005849" MajorTopicYN="N">Gerbillinae</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008809" MajorTopicYN="N">Mice, Inbred C3H</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008819" MajorTopicYN="N">Mice, Nude</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008842" MajorTopicYN="N">Microfilariae</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009693" MajorTopicYN="N">Nucleic Acid Hybridization</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012313" MajorTopicYN="N">RNA</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013045" MajorTopicYN="N">Species Specificity</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
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A DNA probe cloned in Escherichia coli for the identification of Brugia malayi.

A DNA probe cloned in Escherichia coli for the identification of Brugia malayi.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki