Wb E34 monoclonal antibody: further characterization and diagnostic use in bancroftian filariasis.
Identifieur interne : 006061 ( PubMed/Corpus ); précédent : 006060; suivant : 006062Wb E34 monoclonal antibody: further characterization and diagnostic use in bancroftian filariasis.
Auteurs : M V Reddy ; K A Parkhe ; W F Piessens ; B C HarinathSource :
- Journal of clinical laboratory analysis [ 0887-8013 ] ; 1989.
English descriptors
- KwdEn :
- Animals, Antibodies, Helminth (blood), Antibodies, Monoclonal, Antigens, Helminth (immunology), Blotting, Western, Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (immunology), Enzyme-Linked Immunosorbent Assay, Filariasis, Humans, Larva (immunology), Wuchereria (immunology), Wuchereria bancrofti (immunology).
- MESH :
- chemical , blood : Antibodies, Helminth.
- chemical , immunology : Antigens, Helminth.
- diagnosis : Elephantiasis, Filarial.
- immunology : Elephantiasis, Filarial, Larva, Wuchereria, Wuchereria bancrofti.
- Animals, Antibodies, Monoclonal, Blotting, Western, Enzyme-Linked Immunosorbent Assay, Filariasis, Humans.
Abstract
The Wb E34 monoclonal antibody raised against Wuchereria bancrofti microfilarial excretory secretory (mf ES) antigen was reported to be useful in detecting the filarial antigen in W. bancrofti and Brugia malayi infected sera. Further studies in this laboratory showed that this monoclonal antibody reacts with a stage-specific antigen of W. bancrofti filarial parasite. Wb E34 identified three antigenic components with molecular weights, 55, 57.5, and 63 kilodaltons (Kd) in western blot analysis. The target antigen of Wb E34 was found to be located in the cytoplasm of microfilariae by indirect immunofluorescence technique. The inhibitory antibodies to Wb E34 were detected in a higher percentage of microfilaraemic sera (81%) than in clinical filarial (33%) or tropical eosinophilia sera (35%). Thus the inhibition ELISA using W. bancrofti mf ES antigen and Wb E34-penicillinase may be useful in detecting the filarial antibody associated with the active stage of infection.
PubMed: 2681621
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pubmed:2681621Le document en format XML
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Further studies in this laboratory showed that this monoclonal antibody reacts with a stage-specific antigen of W. bancrofti filarial parasite. Wb E34 identified three antigenic components with molecular weights, 55, 57.5, and 63 kilodaltons (Kd) in western blot analysis. The target antigen of Wb E34 was found to be located in the cytoplasm of microfilariae by indirect immunofluorescence technique. The inhibitory antibodies to Wb E34 were detected in a higher percentage of microfilaraemic sera (81%) than in clinical filarial (33%) or tropical eosinophilia sera (35%). Thus the inhibition ELISA using W. bancrofti mf ES antigen and Wb E34-penicillinase may be useful in detecting the filarial antibody associated with the active stage of infection.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">2681621</PMID><DateCreated><Year>1989</Year><Month>11</Month><Day>28</Day></DateCreated><DateCompleted><Year>1989</Year><Month>11</Month><Day>28</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0887-8013</ISSN><JournalIssue CitedMedium="Print"><Volume>3</Volume><Issue>5</Issue><PubDate><Year>1989</Year></PubDate></JournalIssue><Title>Journal of clinical laboratory analysis</Title><ISOAbbreviation>J. Clin. Lab. Anal.</ISOAbbreviation></Journal><ArticleTitle>Wb E34 monoclonal antibody: further characterization and diagnostic use in bancroftian filariasis.</ArticleTitle><Pagination><MedlinePgn>277-81</MedlinePgn></Pagination><Abstract><AbstractText>The Wb E34 monoclonal antibody raised against Wuchereria bancrofti microfilarial excretory secretory (mf ES) antigen was reported to be useful in detecting the filarial antigen in W. bancrofti and Brugia malayi infected sera. Further studies in this laboratory showed that this monoclonal antibody reacts with a stage-specific antigen of W. bancrofti filarial parasite. Wb E34 identified three antigenic components with molecular weights, 55, 57.5, and 63 kilodaltons (Kd) in western blot analysis. The target antigen of Wb E34 was found to be located in the cytoplasm of microfilariae by indirect immunofluorescence technique. The inhibitory antibodies to Wb E34 were detected in a higher percentage of microfilaraemic sera (81%) than in clinical filarial (33%) or tropical eosinophilia sera (35%). Thus the inhibition ELISA using W. bancrofti mf ES antigen and Wb E34-penicillinase may be useful in detecting the filarial antibody associated with the active stage of infection.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Reddy</LastName><ForeName>M V</ForeName><Initials>MV</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Maharashtra, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Parkhe</LastName><ForeName>K A</ForeName><Initials>KA</Initials></Author><Author ValidYN="Y"><LastName>Piessens</LastName><ForeName>W F</ForeName><Initials>WF</Initials></Author><Author ValidYN="Y"><LastName>Harinath</LastName><ForeName>B C</ForeName><Initials>BC</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Clin Lab Anal</MedlineTA><NlmUniqueID>8801384</NlmUniqueID><ISSNLinking>0887-8013</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000909">Antibodies, Helminth</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000911">Antibodies, Monoclonal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000947">Antigens, Helminth</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000909" MajorTopicYN="N">Antibodies, Helminth</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000911" MajorTopicYN="Y">Antibodies, Monoclonal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000947" MajorTopicYN="N">Antigens, Helminth</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015153" MajorTopicYN="N">Blotting, Western</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004605" MajorTopicYN="N">Elephantiasis, Filarial</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005368" MajorTopicYN="N">Filariasis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007814" MajorTopicYN="N">Larva</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014957" MajorTopicYN="N">Wuchereria</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014958" MajorTopicYN="N">Wuchereria bancrofti</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1989</Year><Month>1</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1989</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1989</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">2681621</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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