Wb E34 monoclonal antibody: further characterization and diagnostic use in bancroftian filariasis.
Identifieur interne : 006061 ( PubMed/Corpus ); précédent : 006060; suivant : 006062Wb E34 monoclonal antibody: further characterization and diagnostic use in bancroftian filariasis.
Auteurs : M V Reddy ; K A Parkhe ; W F Piessens ; B C HarinathSource :
- Journal of clinical laboratory analysis [ 0887-8013 ] ; 1989.
English descriptors
- KwdEn :
- Animals, Antibodies, Helminth (blood), Antibodies, Monoclonal, Antigens, Helminth (immunology), Blotting, Western, Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (immunology), Enzyme-Linked Immunosorbent Assay, Filariasis, Humans, Larva (immunology), Wuchereria (immunology), Wuchereria bancrofti (immunology).
- MESH :
- chemical , blood : Antibodies, Helminth.
- chemical , immunology : Antigens, Helminth.
- diagnosis : Elephantiasis, Filarial.
- immunology : Elephantiasis, Filarial, Larva, Wuchereria, Wuchereria bancrofti.
- Animals, Antibodies, Monoclonal, Blotting, Western, Enzyme-Linked Immunosorbent Assay, Filariasis, Humans.
Abstract
The Wb E34 monoclonal antibody raised against Wuchereria bancrofti microfilarial excretory secretory (mf ES) antigen was reported to be useful in detecting the filarial antigen in W. bancrofti and Brugia malayi infected sera. Further studies in this laboratory showed that this monoclonal antibody reacts with a stage-specific antigen of W. bancrofti filarial parasite. Wb E34 identified three antigenic components with molecular weights, 55, 57.5, and 63 kilodaltons (Kd) in western blot analysis. The target antigen of Wb E34 was found to be located in the cytoplasm of microfilariae by indirect immunofluorescence technique. The inhibitory antibodies to Wb E34 were detected in a higher percentage of microfilaraemic sera (81%) than in clinical filarial (33%) or tropical eosinophilia sera (35%). Thus the inhibition ELISA using W. bancrofti mf ES antigen and Wb E34-penicillinase may be useful in detecting the filarial antibody associated with the active stage of infection.
PubMed: 2681621
Links to Exploration step
pubmed:2681621Le document en format XML
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<author><name sortKey="Reddy, M V" sort="Reddy, M V" uniqKey="Reddy M" first="M V" last="Reddy">M V Reddy</name>
<affiliation><nlm:affiliation>Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Maharashtra, India.</nlm:affiliation>
</affiliation>
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<author><name sortKey="Parkhe, K A" sort="Parkhe, K A" uniqKey="Parkhe K" first="K A" last="Parkhe">K A Parkhe</name>
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<author><name sortKey="Piessens, W F" sort="Piessens, W F" uniqKey="Piessens W" first="W F" last="Piessens">W F Piessens</name>
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<author><name sortKey="Harinath, B C" sort="Harinath, B C" uniqKey="Harinath B" first="B C" last="Harinath">B C Harinath</name>
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<author><name sortKey="Reddy, M V" sort="Reddy, M V" uniqKey="Reddy M" first="M V" last="Reddy">M V Reddy</name>
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<series><title level="j">Journal of clinical laboratory analysis</title>
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<term>Antibodies, Helminth (blood)</term>
<term>Antibodies, Monoclonal</term>
<term>Antigens, Helminth (immunology)</term>
<term>Blotting, Western</term>
<term>Elephantiasis, Filarial (diagnosis)</term>
<term>Elephantiasis, Filarial (immunology)</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Filariasis</term>
<term>Humans</term>
<term>Larva (immunology)</term>
<term>Wuchereria (immunology)</term>
<term>Wuchereria bancrofti (immunology)</term>
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<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Helminth</term>
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<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Helminth</term>
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<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term>
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<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Elephantiasis, Filarial</term>
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<term>Wuchereria</term>
<term>Wuchereria bancrofti</term>
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<term>Antibodies, Monoclonal</term>
<term>Blotting, Western</term>
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<front><div type="abstract" xml:lang="en">The Wb E34 monoclonal antibody raised against Wuchereria bancrofti microfilarial excretory secretory (mf ES) antigen was reported to be useful in detecting the filarial antigen in W. bancrofti and Brugia malayi infected sera. Further studies in this laboratory showed that this monoclonal antibody reacts with a stage-specific antigen of W. bancrofti filarial parasite. Wb E34 identified three antigenic components with molecular weights, 55, 57.5, and 63 kilodaltons (Kd) in western blot analysis. The target antigen of Wb E34 was found to be located in the cytoplasm of microfilariae by indirect immunofluorescence technique. The inhibitory antibodies to Wb E34 were detected in a higher percentage of microfilaraemic sera (81%) than in clinical filarial (33%) or tropical eosinophilia sera (35%). Thus the inhibition ELISA using W. bancrofti mf ES antigen and Wb E34-penicillinase may be useful in detecting the filarial antibody associated with the active stage of infection.</div>
</front>
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<Title>Journal of clinical laboratory analysis</Title>
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<ArticleTitle>Wb E34 monoclonal antibody: further characterization and diagnostic use in bancroftian filariasis.</ArticleTitle>
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<Abstract><AbstractText>The Wb E34 monoclonal antibody raised against Wuchereria bancrofti microfilarial excretory secretory (mf ES) antigen was reported to be useful in detecting the filarial antigen in W. bancrofti and Brugia malayi infected sera. Further studies in this laboratory showed that this monoclonal antibody reacts with a stage-specific antigen of W. bancrofti filarial parasite. Wb E34 identified three antigenic components with molecular weights, 55, 57.5, and 63 kilodaltons (Kd) in western blot analysis. The target antigen of Wb E34 was found to be located in the cytoplasm of microfilariae by indirect immunofluorescence technique. The inhibitory antibodies to Wb E34 were detected in a higher percentage of microfilaraemic sera (81%) than in clinical filarial (33%) or tropical eosinophilia sera (35%). Thus the inhibition ELISA using W. bancrofti mf ES antigen and Wb E34-penicillinase may be useful in detecting the filarial antibody associated with the active stage of infection.</AbstractText>
</Abstract>
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