Diagnostic use of polyclonal antibodies raised in mouse ascitic fluid in bancroftian filariasis.
Identifieur interne : 005E56 ( PubMed/Corpus ); précédent : 005E55; suivant : 005E57Diagnostic use of polyclonal antibodies raised in mouse ascitic fluid in bancroftian filariasis.
Auteurs : K. Cheirmaraj ; M V Reddy ; B C HarinathSource :
- Journal of immunoassay [ 0197-1522 ] ; 1990.
English descriptors
- KwdEn :
- Animals, Antibodies, Helminth (isolation & purification), Antigens, Helminth (blood), Brugia (immunology), Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (immunology), Enzyme-Linked Immunosorbent Assay, Humans, Immunologic Tests, Mice, Microfilariae (immunology), Wuchereria bancrofti (immunology).
- MESH :
- chemical , blood : Antigens, Helminth.
- chemical , isolation & purification : Antibodies, Helminth.
- diagnosis : Elephantiasis, Filarial.
- immunology : Brugia, Elephantiasis, Filarial, Microfilariae, Wuchereria bancrofti.
- Animals, Enzyme-Linked Immunosorbent Assay, Humans, Immunologic Tests, Mice.
Abstract
Polyclonal antibodies were produced against Brugia malayi adult antigens (BmA (PBS) SAg and BmA (SDS) SAg) in mouse ascitic fluid by immunising Balb/c mice intraperitoneally with high ratio of adjuvant to immunogen. The diagnostic use of these antibodies in detecting circulating filarial antigen in bancroftian filariasis was studied by sandwich enzyme-linked immunosorbent assay (sandwich ELISA) using stick assay system. Both antibodies raised against PBS and SDS soluble antigens were found to be equally sensitive and relatively specific in detection of circulating filarial antigen. When anti BmA (PBS) SAg antibody was used in sandwich ELISA, 90% of microfilaraemic sera, 30-40% of acute and sub acute filarial sera, 20% of chronic filarial sera, 7% of endemic normal sera and none of 15 non-endemic normal sera were positive for filarial antigen. Using anti BmA (SDS) Sag antibody, 93% of microfilarial sera, 40% of acute and sub acute filarial sera, 20% of chronic filarial sera and none of 15 endemic and non-endemic normal sera showed the presence of filarial antigen. The filarial antigen detection using anti BmA S Ag antibodies produced in mouse ascitic fluid in sandwich ELISA may be useful in detection of active stage (microfilaraemia) of infection.
DOI: 10.1080/01971529008055043
PubMed: 2283388
Links to Exploration step
pubmed:2283388Le document en format XML
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<author><name sortKey="Cheirmaraj, K" sort="Cheirmaraj, K" uniqKey="Cheirmaraj K" first="K" last="Cheirmaraj">K. Cheirmaraj</name>
<affiliation><nlm:affiliation>Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha, India.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Reddy, M V" sort="Reddy, M V" uniqKey="Reddy M" first="M V" last="Reddy">M V Reddy</name>
</author>
<author><name sortKey="Harinath, B C" sort="Harinath, B C" uniqKey="Harinath B" first="B C" last="Harinath">B C Harinath</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Diagnostic use of polyclonal antibodies raised in mouse ascitic fluid in bancroftian filariasis.</title>
<author><name sortKey="Cheirmaraj, K" sort="Cheirmaraj, K" uniqKey="Cheirmaraj K" first="K" last="Cheirmaraj">K. Cheirmaraj</name>
<affiliation><nlm:affiliation>Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha, India.</nlm:affiliation>
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<author><name sortKey="Reddy, M V" sort="Reddy, M V" uniqKey="Reddy M" first="M V" last="Reddy">M V Reddy</name>
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<author><name sortKey="Harinath, B C" sort="Harinath, B C" uniqKey="Harinath B" first="B C" last="Harinath">B C Harinath</name>
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<series><title level="j">Journal of immunoassay</title>
<idno type="ISSN">0197-1522</idno>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Antibodies, Helminth (isolation & purification)</term>
<term>Antigens, Helminth (blood)</term>
<term>Brugia (immunology)</term>
<term>Elephantiasis, Filarial (diagnosis)</term>
<term>Elephantiasis, Filarial (immunology)</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Humans</term>
<term>Immunologic Tests</term>
<term>Mice</term>
<term>Microfilariae (immunology)</term>
<term>Wuchereria bancrofti (immunology)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antigens, Helminth</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antibodies, Helminth</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Brugia</term>
<term>Elephantiasis, Filarial</term>
<term>Microfilariae</term>
<term>Wuchereria bancrofti</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
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<term>Immunologic Tests</term>
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<front><div type="abstract" xml:lang="en">Polyclonal antibodies were produced against Brugia malayi adult antigens (BmA (PBS) SAg and BmA (SDS) SAg) in mouse ascitic fluid by immunising Balb/c mice intraperitoneally with high ratio of adjuvant to immunogen. The diagnostic use of these antibodies in detecting circulating filarial antigen in bancroftian filariasis was studied by sandwich enzyme-linked immunosorbent assay (sandwich ELISA) using stick assay system. Both antibodies raised against PBS and SDS soluble antigens were found to be equally sensitive and relatively specific in detection of circulating filarial antigen. When anti BmA (PBS) SAg antibody was used in sandwich ELISA, 90% of microfilaraemic sera, 30-40% of acute and sub acute filarial sera, 20% of chronic filarial sera, 7% of endemic normal sera and none of 15 non-endemic normal sera were positive for filarial antigen. Using anti BmA (SDS) Sag antibody, 93% of microfilarial sera, 40% of acute and sub acute filarial sera, 20% of chronic filarial sera and none of 15 endemic and non-endemic normal sera showed the presence of filarial antigen. The filarial antigen detection using anti BmA S Ag antibodies produced in mouse ascitic fluid in sandwich ELISA may be useful in detection of active stage (microfilaraemia) of infection.</div>
</front>
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<Month>03</Month>
<Day>15</Day>
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<DateCompleted><Year>1991</Year>
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<DateRevised><Year>2016</Year>
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<Article PubModel="Print"><Journal><ISSN IssnType="Print">0197-1522</ISSN>
<JournalIssue CitedMedium="Print"><Volume>11</Volume>
<Issue>4</Issue>
<PubDate><Year>1990</Year>
</PubDate>
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<Title>Journal of immunoassay</Title>
<ISOAbbreviation>J Immunoassay</ISOAbbreviation>
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<ArticleTitle>Diagnostic use of polyclonal antibodies raised in mouse ascitic fluid in bancroftian filariasis.</ArticleTitle>
<Pagination><MedlinePgn>429-44</MedlinePgn>
</Pagination>
<Abstract><AbstractText>Polyclonal antibodies were produced against Brugia malayi adult antigens (BmA (PBS) SAg and BmA (SDS) SAg) in mouse ascitic fluid by immunising Balb/c mice intraperitoneally with high ratio of adjuvant to immunogen. The diagnostic use of these antibodies in detecting circulating filarial antigen in bancroftian filariasis was studied by sandwich enzyme-linked immunosorbent assay (sandwich ELISA) using stick assay system. Both antibodies raised against PBS and SDS soluble antigens were found to be equally sensitive and relatively specific in detection of circulating filarial antigen. When anti BmA (PBS) SAg antibody was used in sandwich ELISA, 90% of microfilaraemic sera, 30-40% of acute and sub acute filarial sera, 20% of chronic filarial sera, 7% of endemic normal sera and none of 15 non-endemic normal sera were positive for filarial antigen. Using anti BmA (SDS) Sag antibody, 93% of microfilarial sera, 40% of acute and sub acute filarial sera, 20% of chronic filarial sera and none of 15 endemic and non-endemic normal sera showed the presence of filarial antigen. The filarial antigen detection using anti BmA S Ag antibodies produced in mouse ascitic fluid in sandwich ELISA may be useful in detection of active stage (microfilaraemia) of infection.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cheirmaraj</LastName>
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<MedlineTA>J Immunoassay</MedlineTA>
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<CommentsCorrectionsList><CommentsCorrections RefType="ErratumIn"><RefSource>J Immunoassay 1991;12(4):611-2</RefSource>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
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<MeshHeading><DescriptorName UI="D000909" MajorTopicYN="Y">Antibodies, Helminth</DescriptorName>
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<MeshHeading><DescriptorName UI="D004605" MajorTopicYN="N">Elephantiasis, Filarial</DescriptorName>
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<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
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<MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName>
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<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
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<MeshHeading><DescriptorName UI="D007159" MajorTopicYN="N">Immunologic Tests</DescriptorName>
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<MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName>
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<MeshHeading><DescriptorName UI="D008842" MajorTopicYN="N">Microfilariae</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
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<MeshHeading><DescriptorName UI="D014958" MajorTopicYN="N">Wuchereria bancrofti</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
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